scholarly journals Hexose uptake

2007 ◽  
Vol 20 ◽  
Author(s):  
P D'Eustachio
Keyword(s):  
Hexose Uptake

Kinetic parameters of hexose transport in hybrids between malignant and nonmalignant cells

1983 ◽  
Vol 62 (1) ◽  
pp. 49-80
Author(s):  
M.K. White ◽  
M.E. Bramwell ◽  
H. Harris
Keyword(s):  
Kinetic Parameters ◽  
Malignant Cell ◽  
Hexose Transport ◽  
Hybrid Cells ◽  
Michaelis Constant ◽  
Independent Measurement ◽  
The Difference ◽  
Hexose Uptake ◽  
Derivatives Of

Matched pairs of isogeneic hybrid cells, in which one member of the pair was malignant and the other not, were used to examine the linkage between malignancy and functional alterations in hexose transport. The kinetic parameters of uptake of 2-deoxy-D-glucose were measured in a range of such hybrids, both human and murine. Some other malignant cell lines were also examined and were compared with non-tumorigenic derivatives of tumour cells selected by exposure to the lectin, wheat-germ agglutinin. In every case, malignancy, as defined by the ability of cells to grow progressively in vivo, was found to be linked to a decrease in the Michaelis constant of hexose uptake. Independent measurement of the transport and phosphorylation reactions involved in hexose uptake revealed that this decrease was determined by the membrane transport system. The difference in Michaelis constant between malignant and non-malignant cells was observed with 3-O-methylglucose, a hexose that is transported into the cell but not further metabolized. The activity of hexokinase in cell homogenates was higher than the level that would be required to cope with transport and showed no correlation with tumorigenicity. Measurement of the uptake of D-glucose itself, by a rapid filtration centrifugation method, gave results similar to those obtained with 2-deoxy-D-glucose.

scholarly journals Editorial: Hexose Uptake and Metabolism in Immune Homeostasis and Inflammation

2022 ◽  
Vol 12 ◽  
Author(s):  
Dunfang Zhang ◽  
Chaohong Liu ◽  
Hiroko Nakatsukasa ◽  
WanJun Chen
Keyword(s):  
Immune Homeostasis ◽  
Hexose Uptake ◽  
Uptake And Metabolism

Amino acid and hexose transport by cultured crypt cells from rat small intestine

1980 ◽  
Vol 239 (5) ◽  
pp. C190-C196 ◽  
Author(s):  
K. Inui ◽  
A. Quaroni ◽  
L. G. Tillotson ◽  
K. J. Isselbacher
Keyword(s):  
Amino Acid ◽  
Sugar Transport ◽  
Epithelial Cell Line ◽  
Acid Uptake ◽  
Substrate Uptake ◽  
Hexose Transport ◽  
Intestinal Crypt ◽  
Morphological Criteria ◽  
Hexose Uptake ◽  
Crypt Cells

The characteristics of amino acid and sugar transport in intestinal crypt epithelial cells have been examined by measuring substrate uptake in an established epithelial cell line. These cells (IEC-6 cells) have been characterized as derived from rat small intestinal crypt cells on the basis of morphological criteria (J. Cell. Biol. 80: 248-265, 1979). Amino acid transport appeared to be mediated by both Na+-dependent and Na+-independent systems. Hexose uptake was stereospecific and Na+ independent, and was markedly inhibited by phloretin and cytochalasin B. Since glucocorticoids are known to have profound effects on maturation of the intestinal epithelium in vivo, their effects on transport properties of the cultured crypt cells were studied. Hydrocortisone, while completely inhibiting cell growth, increased the initial uptake rates of various hexoses, while having little or nor effect on the initial rate of amino acid uptake. The increased hexose uptake appeared to be due to a change in Vmax rather than Km. Appearance of the Na+-dependent hexose transport system, which is present in differentiated enterocytes, was not elicited by in vitro treatment with glucocortcoids.

Effects of adenosine antagonists on hexose uptake and preconditioning in perfused rat heart

1993 ◽  
Vol 265 (4) ◽  
pp. C1146-C1155 ◽  
Author(s):  
E. Murphy ◽  
T. A. Fralix ◽  
R. E. London ◽  
C. Steenbergen
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Beneficial Effect ◽  
Recovery Of Function ◽  
Anaerobic Glycolysis ◽  
Perfused Rat Heart ◽  
Adenosine Antagonists ◽  
Adenosine Antagonist ◽  
Ionic Changes ◽  
Hexose Uptake

Preconditioning with brief intermittent periods of ischemia has been shown to lessen the detrimental effects of a subsequent sustained (30-60 min) period of ischemia. Because adenosine has been suggested to be the mediator of preconditioning, we were interested in investigating whether adenosine antagonists would block the effect of preconditioning on ionic changes during ischemia. We found that 10 microM of the adenosine antagonist BW-A1433U did not reverse the effect of preconditioning on intracellular pH (pHi). Hearts preconditioned with BW-A1433U had virtually no decrease in pHi during the 30-min sustained period of ischemia; after 30 min of ischemia, the pH in untreated hearts was 5.97 +/- 0.16 compared with 6.52 +/- 0.10 in preconditioned hearts and 6.90 +/- 0.08 in hearts preconditioned plus BW-A1433U. Because anaerobic glycolysis is largely responsible for the fall in pHi during ischemia, we examined the effect of BW-A1433U [and other adenosine antagonists, such as PD-115,199 and 8-cyclopentyl-1,3-dipropylxanthine (CPDPX)] on glucose uptake and phosphorylation during aerobic perfusion using 31P-nuclear magnetic resonance to monitor uptake and phosphorylation of 2-deoxyglucose (2-DG) to 2-deoxyglucose 6-phosphate (2-DG-6-P) when one-half of the glucose in the perfusate was replaced with 2-DG. Uptake of 2-DG-6-P after 15 min was reduced by 66% in the presence of BW-A1433U and 82% in the presence of PD-115,199 as compared with untreated hearts, but was not reduced in the presence of CPDPX. Thus CPDPX was the only adenosine antagonist tested that did not block accumulation of 2-DG-6-P. We also found that CPDPX did not block the beneficial effect of preconditioning on ionic alterations during a sustained 30-min period of ischemia or the improved recovery of function on reflow.

Direct evidence for protein kinase c involvement in insulin-stimulated hexose uptake

1992 ◽  
Vol 188 (1) ◽  
pp. 142-148 ◽  
Author(s):  
Denise R. Cooper ◽  
James E. Watson ◽  
Herman Hernandez ◽  
Bingzhi Yu ◽  
Mary L. Standaert ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Direct Evidence ◽  
Kinase C ◽  
Hexose Uptake

scholarly journals Effects of sodium butyrate on the membrane glycoconjugates of murine sarcoma virus-transformed rat cells.

1980 ◽  
Vol 84 (2) ◽  
pp. 225-234 ◽  
Author(s):  
D P Via ◽  
S Sramek ◽  
G Larriba ◽  
S Steiner
Keyword(s):  
Biochemical Markers ◽  
Early Stage ◽  
Ganglioside Gm3 ◽  
Substrate Adhesion ◽  
Cell Substrate ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Hexose Uptake ◽  
Cell Substrate Adhesion ◽  
Murine Sarcoma

The temporal relationship between butyrate-induced cellular flattening of murine sarcoma virus-transformed rat cells (MSV-NRK) and alterations in certain surface-associated biochemical markers of transformation, e.g., surface glycopeptides, glycolipids, fibronectin, hexose uptake, and cell-substrate adhesion was examined. The induction of elevated levels of the ganglioside GM3 and of a GDla-like ganglioside were observed to precede or to parallel cellular flattening. Likewise, enhanced incorporation of radioisotopically labeled fucose into a novel fucose-containing component, i.e., glucopyranosyl (1 leads to 3) fucopyranosyl-threonine, was also observed to occur at an early stage of cellular flattening. In contrast, a shift in the molecular weight distribution of trypsin-sensitive, surface fucopeptides was observed to occur at a late stage of cellular flattening. Moreover, surface fibronectin was not detectable in the butyrate-flattened MSV-NRK cells despite the fact that the cells manifested significantly enhanced cell-substrate adhesion. Thus, butyrate appears to be a useful tool for understanding the sequential changes associated with expression of the transformed phenotype of MSV-NRK cells.

Long-term regulation of hexose uptake by isoproterenol in cultured 3T3 adipocytes

1985 ◽  
Vol 248 (6) ◽  
pp. E706-E711 ◽  
Author(s):  
J. P. van Putten ◽  
H. M. Krans
Keyword(s):  
Protein Synthesis ◽  
Prolonged Exposure ◽  
Term Effect ◽  
Long Term Effects ◽  
Fat Cell ◽  
Short Term ◽  
Long Term Effect ◽  
Short Term Exposure ◽  
Hexose Uptake

Catecholamines are known to have short-term regulatory effects on fat cell hexose uptake. We examined the long-term effects of catecholamines on the insulin-sensitive 2-deoxyglucose (dGlc) uptake in cultured 3T3-L1 adipocytes. Prolonged exposure (48 h) to isoproterenol (beta-adrenergic agonist) stimulated the basal dGlc uptake up to 90%. The effect was specific, time, concentration, and protein synthesis dependent and reversible. The effect of insulin was unaltered and superimposed on the increase in basal dGlc uptake. The long-term effect of isoproterenol was mimicked by epinephrine, dibutyryl cAMP (DBcAMP), and 1-methyl-3-isobutylxanthine (IBMX). By contrast, short-term exposure to isoproterenol (and epinephrine) induced a protein synthesis-independent increase in basal dGlc uptake (30%) not accompanied by an increase in insulin responsiveness. Moreover, on short-term basis, DBcAMP and IBMX suppressed both the basal and insulin-stimulated uptake up to 50%. Determination of the intracellular nonphosphorylated dGlc during the uptake and of the hexokinase activity revealed that the long-term effect of isoproterenol was most likely due to alterations low in dGlc transport. In conclusion, long-term regulators of hexose uptake are in cultured 3T3-L1 adipocytes, isoproterenol, and other cAMP stimulators. The long-term effect is independent from the short-term regulatory effect of the agents and from the effect of insulin.

Stimulation of Na+/H+Exchange by Insulin and Phorbol Ester during Differentiation of 3T3-L1 Cells. Relation to Hexose Uptake*

Endocrinology ◽  
1988 ◽  
Vol 123 (1) ◽  
pp. 296-304 ◽  
Author(s):  
AMIRA KLIP ◽  
TOOLSIE RAMLAL ◽  
ULLA-MAIJA KOIVISTO
Keyword(s):  
Phorbol Ester ◽  
Hexose Uptake ◽  
Stimulation Of

Cyclic AMP does not modulate hexose uptake of serum-deprived BHK-21 fibroblasts

1982 ◽  
Vol 112 (3) ◽  
pp. 373-375
Author(s):  
Victor R. Lavis ◽  
Roxann Davenport ◽  
Paul Pontier ◽  
W. Joseph Thompson ◽  
Samuel J. Strada
Keyword(s):  
Cyclic Amp ◽  
Hexose Uptake
Sign in / Sign up

Export Citation Format

Share Document