Latent Viral Infection of Cells in Tissue Culture. IX. Abortive Infection with Psittacosis Virus.

By maintaining L cells in a balanced salt solution of inorganic salts and glucose (BSS) for 2 days or more, they are rendered incapable of supporting the growth of psittacosis virus (6BC), though it infects such cells and is present intracellularly for as long as 3 days in a non-infectious phase. The addition of an enriched medium to such a culture of cells at any time up to 4 days after infection results in the appearance of infectious virus within these cells, which multiplies and is released from the cells, providing the entire period of exposure of such cells to the BSS does not exceed 6 days, following which the cells die. A latent infection with psittacosis virus in a non-infectious phase has been established in a pure line of cells which possess properties of malignancy.

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Gammaherpesvirus infection licenses age-associated B cells for pathogenicity in MS and EAE

10.1101/2021.07.22.453263 ◽

2021 ◽

Author(s):

Isobel C. Mouat ◽

Jessica R. Allanach ◽

Vina Fan ◽

Anna M. Girard ◽

Iryna Shanina ◽

...

Keyword(s):

B Cells ◽

Viral Infection ◽

Viral Infections ◽

Autoimmune Encephalomyelitis ◽

Barr Virus ◽

Ebv Infection ◽

Latent Viral Infection ◽

Epstein Barr ◽

Gammaherpesvirus 68 ◽

Experimental Autoimmune

While age-associated B cells (ABCs) are known to expand and persist following viral infection and during autoimmunity, their interactions are yet to be studied together in these contexts. Epstein-Barr virus (EBV) infection has long been implicated in multiple sclerosis (MS), and it is not known whether ABCs could play a role in mediating viral contribution to autoimmunity. Here, we show that the circulating ABC population is expanded in people with MS and that EBV infection and MS status differentially impact the circulating ABC phenotype. We then directly compared ABCs during viral infection and autoimmunity using mouse models of EBV, gammaherpesvirus 68 (γHV68), and MS, experimental autoimmune encephalomyelitis (EAE). We observed that splenic ABCs are expanded in a sex-biased manner during both latent virus infection and EAE, and each event drives the ABC population to opposing phenotypes. We have previously shown that latent γHV68 infection exacerbates EAE and here we show that mice lacking ABCs fail to display γHV68-enhanced disease. Collectively, these findings indicate that latent viral infection and central nervous system autoimmunity differentially impact the ABC population and suggests that viral infections such as EBV prime ABCs to contribute pathogenically in MS.

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T Cell Repertoire Maturation Induced by Persistent and Latent Viral Infection Is Insufficient to Induce Costimulation Blockade Resistant Organ Allograft Rejection in Mice

Frontiers in Immunology ◽

10.3389/fimmu.2018.01371 ◽

2018 ◽

Vol 9 ◽

Author(s):

Jaclyn R. Espinosa ◽

Danny Mou ◽

Bartley W. Adams ◽

Louis R. DiBernardo ◽

Andrea L. MacDonald ◽

...

Keyword(s):

T Cell ◽

Viral Infection ◽

Allograft Rejection ◽

T Cell Repertoire ◽

Costimulation Blockade ◽

Cell Repertoire ◽

Latent Viral Infection

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Influence of Growing Medium on the Parameters and Yield Capacity of the Mini Tuber Potato of the Charoite Variety

KnE Life Sciences ◽

10.18502/kls.v0i0.8994 ◽

2021 ◽

Author(s):

Tamara A. Shelabina ◽

Dmitriy A. Danilov ◽

Aleksandr I. Rodionenkov ◽

Aleksey A. Kuznetsov

Keyword(s):

Viral Infection ◽

Fungal Diseases ◽

Quantitative Yield ◽

Original Seed ◽

Latent Viral Infection ◽

Growing Medium ◽

Growth Development ◽

Yield Capacity ◽

The Creation

The creation of original seed grains of the Charoite variety, healed of pathogenic infections of various etiologies, was carried out through reproduction of the certified meristematic material of the microplants class. Mini tubers microplants were grown in 5.5 liter vessels on a “Agrobalt S” soil (control) mixed with peat and supplemented with 10% and 20% (of the volume) of the “ORVI” substrate. The use of the substrate “ORVI” mixed with peat and “Agrobalt S” soil positively influenced the growth, development and formation of tubers. The appliance of 10% of the “ORVI” substrate significantly increased the quantitative yield of mini tubers by 17% and the weight of tubers by 12.3%. The increase in the total number of mini tubers was due to the increase in tuber fraction from 10 to 30 mm. The appliance of a 20% “ORVI” substrate also led to an increase in the total number and mass of mini tubers. According to the EIA results, the mini tubers grown from microplants did not contain any latent viral infection. Tests on the seeds did not reveal symptoms of fungal diseases. Keywords: breed, potatoes, mini tubers, mass of tubers, peat fertilizer, substrate

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