Intradermal Immune Responses to a Schistosomal Egg Antigen During Experimental Murine Schistosoma mansoni Infection

Abstract Schistosomiasis is an inflammatory disease that occurs when schistosome species eggs are deposited in the liver, resulting in fibrosis and portal hypertension. Schistosomes can interact with host inflammasomes to elicit host immune responses, leading to mitochondrial damage, generation of high levels of reactive oxygen species (ROS) and activation of apoptosis during inflammation. This study aims to examine whether ROS and NF-κB (p65) expression elicited other types of inflammasome activation in Schistosoma mansoni-infected mouse livers. We examine the relationship between inflammasome activation, mitochondrial damage and ROS production in mouse livers infected with S. mansoni. We demonstrate a significant release of ROS and superoxides and increased NF-κB (p65) in S. mansoni-infected mouse livers. Moreover, activation of the NLRP3 and AIM2 inflammasomes was triggered by S. mansoni infection. Stimulation of HuH-7 hepatocellular carcinoma cells with soluble egg antigen induced activation of the AIM2 inflammasome pathway. In this study, we demonstrate that S. mansoni infection promotes both NLRP3 and AIM2 inflammasome activation.

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Cellular immune responses of a Senegalese community recently exposed to Schistosoma mansoni: correlations of infection level with age and inflammatory cytokine production by soluble egg antigen-specific cells

Tropical Medicine & International Health ◽

10.1046/j.1365-3156.1999.00443.x ◽

1999 ◽

Vol 4 (8) ◽

pp. 530-543 ◽

Cited By ~ 18

Author(s):

M. Marguerite ◽

M.-C. Gallissot ◽

M. Diagne ◽

C. Moreau ◽

M.-M. Diakkhate ◽

...

Keyword(s):

Immune Responses ◽

Schistosoma Mansoni ◽

Inflammatory Cytokine ◽

Cytokine Production ◽

Infection Level ◽

Inflammatory Cytokine Production ◽

Cellular Immune Responses ◽

Cellular Immune ◽

Egg Antigen

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Immune Responses in Mice after Immunization with Antigens from Different Stages of the Parasite Schistosoma mansoni

Zeitschrift für Naturforschung C ◽

10.1515/znc-2010-3-419 ◽

2010 ◽

Vol 65 (3-4) ◽

pp. 289-302 ◽

Cited By ~ 4

Author(s):

Hanaa M. Gaber ◽

Amany S. Maghraby ◽

Mohamed Bastawy Ahmed ◽

Andreas Ruppel ◽

Mahmoud M. Bahgat

Keyword(s):

T Cells ◽

Immune Responses ◽

Lymph Nodes ◽

Schistosoma Mansoni ◽

B Lymphocytes ◽

T And B Lymphocytes ◽

Mesenteric Lymph Nodes ◽

Antigen Preparation ◽

Mesenteric Lymph ◽

Egg Antigen

Mice responses to immunization with Schistosoma mansoni antigens were investigated. Priming with cercarial antigen preparation (CAP) induced significant (P < 0.05) IgM, IgG, IgG2a, IgG2b, and IgA increases, while booster caused a significant IgG1 increase. A soluble worm antigen preparation (SWAP) caused significant IgG elevation. Priming with soluble egg antigen (SEA) caused significant IgM and IgG2a increases, while booster induced significant IgM, IgG and IgA increases. CAP-immunized mice sera (IMS) recognized CAP peptides ranging from 23 - 78 kDa. SWAP-IMS recognized SWAP peptides ranging from 40 - 75 kDa. SEA-IMS recognized SEA peptides ranging from 33 - 101 kDa. The cross-reactive peptides among the 3 antigens were identified. CAP caused significant increases in mesenteric lymph nodes (MLNs) CD4,8+, B lymphocytes, CD8+ thymocytes, CD4+ T and B splenocytes. SWAP priming caused significant increases in MLNs CD4,8+ thymocytes and B splenocytes. SWAP booster caused significant increases in MLNs CD8+ T and B lymphocytes, CD4,8+ thymocytes and CD4+ T and B splenocytes. SEA caused significant increase in CD4+ T cells.

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Immunohistochemical and ultrastructural localization of Schistosoma mansoni soluble egg antigens processed by the infected host

Parasitology ◽

10.1017/s0031182000066117 ◽

1996 ◽

Vol 112 (6) ◽

pp. 537-543 ◽

Cited By ~ 7

Author(s):

J. J. P. M. Bogers ◽

H. A. M. Nibbeling ◽

A. M. Deelder ◽

E. A. E. Van Marck

Keyword(s):

Schistosoma Mansoni ◽

Indirect Evidence ◽

Ultrastructural Localization ◽

Carbohydrate Epitopes ◽

Eosinophilic Granulocytes ◽

Antigenic Material ◽

Egg Shells ◽

Secondary Lysosomes ◽

Antigen Antibody ◽

Egg Antigen

SUMMARYThe detection of egg-derived antigens in the serum and urine of Schistosoma mansoni-infected individuals and experimental animals would provide an alternative method to assess the tissue egg burden. The detected levels are, however, not only a function of the amounts of antigen produced, but also of the processing or clearance by the host. In the present study the immunolocalization pattern of antigens using 2 recently described monoclonal antibodies to repetitive carbohydrate epitopes of S. mansoni soluble egg antigen (114–5B1–A and 114–4D12–A) in various organs of the host was investigated. In the liver strong immunoreactivity could be detected around the entrapped eggs and in egg-shells, as well as in Kupffer cells accumulating both antigen and schistosomal pigment. In the spleen, immunohistochemistry revealed antigen in the plasma as well as in secondary lysosomes of macrophages. Strong labelling was found in the vesicles of the eosinophilic granulocytes: indirect evidence perhaps for the presence of antigen–antibody complexes. In conclusion, the secreted egg antigens were sequestered in the reticulo-endothelial macrophages of the liver and the spleen as already partly described for worm-derived antigens. The presence of large quantities of antigenic material in the spleen could suggest an important role of this organ in the clearance of antigen and might even provide an additional explanation for the hepatosplenomegaly mainly present in S. mansoni-infected children.

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Do intestinal parasites interfere with the seroepidemiologic surveillance of Schistosoma mansoni infection?

Epidemiology and Infection ◽

10.1017/s095026880005264x ◽

1996 ◽

Vol 116 (3) ◽

pp. 323-329 ◽

Cited By ~ 14

Author(s):

B. Alarcón De Noya ◽

C. Colmenares ◽

S. Losada ◽

Z. Fermin ◽

G. Masroua ◽

...

Keyword(s):

Schistosoma Mansoni ◽

False Positive ◽

Total Population ◽

Intestinal Parasites ◽

Field Work ◽

Cross Reactivity ◽

Cross Reaction ◽

Protein Fractions ◽

Egg Antigen ◽

Immunoenzymatic Assay

SUMMARYIn view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15·3% of the total population as determined by SEA–ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.

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