Immunomodulating effect of Schistosoma mansoni soluble egg antigen on course of induced diabetes mellitus in experimental mice

SUMMARYThe detection of egg-derived antigens in the serum and urine of Schistosoma mansoni-infected individuals and experimental animals would provide an alternative method to assess the tissue egg burden. The detected levels are, however, not only a function of the amounts of antigen produced, but also of the processing or clearance by the host. In the present study the immunolocalization pattern of antigens using 2 recently described monoclonal antibodies to repetitive carbohydrate epitopes of S. mansoni soluble egg antigen (114–5B1–A and 114–4D12–A) in various organs of the host was investigated. In the liver strong immunoreactivity could be detected around the entrapped eggs and in egg-shells, as well as in Kupffer cells accumulating both antigen and schistosomal pigment. In the spleen, immunohistochemistry revealed antigen in the plasma as well as in secondary lysosomes of macrophages. Strong labelling was found in the vesicles of the eosinophilic granulocytes: indirect evidence perhaps for the presence of antigen–antibody complexes. In conclusion, the secreted egg antigens were sequestered in the reticulo-endothelial macrophages of the liver and the spleen as already partly described for worm-derived antigens. The presence of large quantities of antigenic material in the spleen could suggest an important role of this organ in the clearance of antigen and might even provide an additional explanation for the hepatosplenomegaly mainly present in S. mansoni-infected children.

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Do intestinal parasites interfere with the seroepidemiologic surveillance of Schistosoma mansoni infection?

Epidemiology and Infection ◽

10.1017/s095026880005264x ◽

1996 ◽

Vol 116 (3) ◽

pp. 323-329 ◽

Cited By ~ 14

Author(s):

B. Alarcón De Noya ◽

C. Colmenares ◽

S. Losada ◽

Z. Fermin ◽

G. Masroua ◽

...

Keyword(s):

Schistosoma Mansoni ◽

False Positive ◽

Total Population ◽

Intestinal Parasites ◽

Field Work ◽

Cross Reactivity ◽

Cross Reaction ◽

Protein Fractions ◽

Egg Antigen ◽

Immunoenzymatic Assay

SUMMARYIn view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15·3% of the total population as determined by SEA–ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.

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FoxP3+ T regulatory cells and immunomodulation after Schistosoma mansoni egg antigen immunization in experimental model of inflammatory bowel disease

Cellular Immunology ◽

10.1016/j.cellimm.2015.02.013 ◽

2015 ◽

Vol 295 (1) ◽

pp. 67-76 ◽

Cited By ~ 20

Author(s):

Eiman A. Hasby ◽

Marwa A. Hasby Saad ◽

Zeinab Shohieb ◽

Kholoud El Noby

Keyword(s):

Inflammatory Bowel Disease ◽

Schistosoma Mansoni ◽

Experimental Model ◽

Bowel Disease ◽

T Regulatory Cells ◽

Regulatory Cells ◽

Inflammatory Bowel ◽

Egg Antigen

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Antibody response to immunization with Schistosoma mansoni egg antigen-antibody complex

Experimental Parasitology ◽

10.1016/0014-4894(63)90072-9 ◽

1963 ◽

Vol 13 (2) ◽

pp. 204-210 ◽

Cited By ~ 1

Author(s):

Walter Stahl ◽

José Oliver-González ◽

Amina Rivera de Sala

Keyword(s):

Antibody Response ◽

Schistosoma Mansoni ◽

Antibody Complex ◽

Antigen Antibody ◽

Egg Antigen

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Activation of the NLRP3 and AIM2 inflammasomes in a mouse model of Schistosoma mansoni infection

Journal of Helminthology ◽

10.1017/s0022149x19000622 ◽

2019 ◽

Vol 94 ◽

Cited By ~ 4

Author(s):

T.T.W. Chen ◽

P.C. Cheng ◽

K.C. Chang ◽

J.P. Cao ◽

J.L. Feng ◽

...

Keyword(s):

Immune Responses ◽

Schistosoma Mansoni ◽

Infected Mouse ◽

Mitochondrial Damage ◽

Carcinoma Cells ◽

Inflammasome Activation ◽

Host Immune Responses ◽

Significant Release ◽

The Relationship ◽

Egg Antigen

Abstract Schistosomiasis is an inflammatory disease that occurs when schistosome species eggs are deposited in the liver, resulting in fibrosis and portal hypertension. Schistosomes can interact with host inflammasomes to elicit host immune responses, leading to mitochondrial damage, generation of high levels of reactive oxygen species (ROS) and activation of apoptosis during inflammation. This study aims to examine whether ROS and NF-κB (p65) expression elicited other types of inflammasome activation in Schistosoma mansoni-infected mouse livers. We examine the relationship between inflammasome activation, mitochondrial damage and ROS production in mouse livers infected with S. mansoni. We demonstrate a significant release of ROS and superoxides and increased NF-κB (p65) in S. mansoni-infected mouse livers. Moreover, activation of the NLRP3 and AIM2 inflammasomes was triggered by S. mansoni infection. Stimulation of HuH-7 hepatocellular carcinoma cells with soluble egg antigen induced activation of the AIM2 inflammasome pathway. In this study, we demonstrate that S. mansoni infection promotes both NLRP3 and AIM2 inflammasome activation.

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Evaluation of anti-Schistosoma mansoni igG antibodies in patients with chronic schistosomiasis mansoni before and after specific treatment

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652001000300007 ◽

2001 ◽

Vol 43 (3) ◽

pp. 153-159 ◽

Cited By ~ 8

Author(s):

Célia Maria V. VENDRAME ◽

Márcia Dias T. CARVALHO ◽

Célia Regina F. YAMAMOTO ◽

Maria Cristina NAKHLE ◽

Silvino Alves CARVALHO ◽

...

Keyword(s):

Schistosoma Mansoni ◽

Specific Treatment ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Igg Antibodies ◽

Schistosomiasis Mansoni ◽

Before And After ◽

Precipitin Test ◽

Egg Antigen ◽

Adult Worm Antigen

The circumoval precipitin test (COPT), enzyme-linked immunosorbent assay (ELISA) and the immunoblotting anti-adult worm antigen (AWA) and soluble egg antigen (SEA) tests were applied to 17 chronically schistosome-infected patients for the detection of anti-Schistosoma mansoni antibodies before and on four occasions after oxamniquine administration over a period of six months. Compared to a control group, schistosomiasis patients showed high levels of IgG antibodies in AWA and SEA-ELISA. A decrease in IgG levels was observed six months after treatment, although negative reactions were not obtained. Significant decreases in IgG1, IgG3 and, mainly, IgG4, but not anti-SEA IgG2 levels were observed six months after treatment, again without negativity. Analysis of anti-AWA IgG antibodies by immunoblotting before treatment showed a 31 kDa strand in 14 patients (82%) which disappeared in three cases up to six months after treatment; furthermore, anti-SEA IgG antibodies showed the same band in nine patients (53%) before treatment, which disappeared in only four cases up to six months after treatment.

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