DEVELOPMENT AND VALIDATION OF ABSORBANCE CORRECTION METHOD AND FIRST ORDER DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF GALLIC ACID, ELLAGIC ACID AND CURCUMIN IN POLYHERBAL ANTIDIABETIC  DOSAGE FORMS.

Two chemometric methods, Inverse Least Square (ILS) and Classical Least Square (CLS), were applied for the simultaneous estimation of gallic acid, ellagic acid and curcumin in polyherbal antidiabetic formulation. Twenty mixed solutions were prepared for the chemometric calibration as training set and 10 mixed solutions were prepared as validation set. The absorbance data matrix was obtained by measuring the absorbance at 20 different wavelengths, from 241 to 279 nm with the interval of 2 nm (Δλ= 2 nm). The developed calibrations were successfully tested for three antidiabetic polyherbal formulations for their gallic acid, ellagic acid and curcumin contents. Developed methods were validated and root mean square error of precision (RMSEP) was determined. Both chemometric methods in this study can be satisfactorily used for the quantitative analysis in polyherbal dosage forms. The chemometric calculations were performed by using the chemometrics toolbox with MATLAB R2015a software.

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Development and Validation of First-Order Derivative Spectrophotometry for Simultaneous Determination of Levocetirizine Dihydrochloride and Phenylephrine Hydrochloride in Pharmaceutical Dosage Form

International Journal of Spectroscopy ◽

10.1155/2013/502310 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Kaminee Parmar ◽

Sunil Baldania ◽

Dimal Shah ◽

Usmangani Chhalotiya ◽

Naimin Parmar

Keyword(s):

Spectrophotometric Method ◽

Dosage Form ◽

Correlation Coefficients ◽

Order Derivative ◽

Simultaneous Estimation ◽

Pharmaceutical Dosage Form ◽

Zero Crossing ◽

Phenylephrine Hydrochloride ◽

First Order ◽

Crossing Point

A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of levocetirizine dihydrochloride (LCT) and phenylephrine hydrochloride (PHE) by employing first-order derivative spectrophotometric method. The first-order derivative absorption at 240 nm (zero crossing point of PHE) was used for quantification of LCT and 283.2 nm (zero crossing point of LCT) for quantification of PHE. The linearity was established over the concentration range of 4–24 μg/mL and 8–48 μg/mL for LCT and PHE with correlation coefficients (r2) 0.9964 and 0.9972, respectively. The mean % recoveries were found to be in the range of 99.14%–100.43% for LCT and 98.73%–100.83% for PHE. The proposed method has been validated as per ICH guideline and successfully applied for the simultaneous estimation of LCT and PHE in combined tablet dosage form.

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Novel First Order Derivative UV Spectrophotometric Method for the Determination of Glimepiride in Solid Dosage Forms

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.8.3.2 ◽

2018 ◽

Vol 8 (3) ◽

Author(s):

Santosh Karajgi . ◽

Sunayana Mali ◽

Ramaling Kotnal

Keyword(s):

Dosage Forms ◽

Order Derivative ◽

Simultaneous Estimation ◽

Drug Form ◽

Solid Dosage Forms ◽

First Order ◽

Solid Dosage ◽

Tablet Dosage ◽

Guide Lines

Objective: An easy, perfect, specific and exact process has been studied for the simultaneous estimation of Glimepiride pure drug form as well as tablet dosage forms. Methods: A UV method for quantitative evaluation of Glimepiride by first order derivative peak detect method for determination in bulk as well as tablet dosage form is reported as there was a need to expand novel methods to analyze the drug. Results: Glimepiride has absorbance first derivative maxima at 225 nm in Methanol. Glimepiride follows Beer’s law in concentration range of 5-25µg/ml. The outcomes of the study were validated statistically and recovery studies were performed as per ICH guide lines. Conclusion: Thus the projected method can be applied competently for the estimation of Glimepiride in regular analysis in its dosage forms.

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