Isolation and characterization of secondary metabolites from endophytic fungi, Aspergillus tubingensis strain CS/7/2 from leaves of Andrographis paniculata (Burm. f.) Nees

2021 ◽  
Vol 13 (02) ◽  
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Andrographis Paniculata ◽  
Aspergillus Tubingensis ◽  
Isolation And Characterization

scholarly journals Chemical and Biological Investigations of Cerbera odollam Gaertn

2018 ◽  
Vol 16 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Monica Sharfin Rahman ◽  
Adnan Faisal ◽  
Choudhury Mahmood Hasan ◽  
Monira Ahsan ◽  
Mohammad Mehedi Masud
Keyword(s):  
Carbon Tetrachloride ◽  
Secondary Metabolites ◽  
Petroleum Ether ◽  
Chromatographic Separation ◽  
Methanolic Extract ◽  
Scientific Basis ◽  
Separation And Purification ◽  
Isolation And Characterization ◽  
Soluble Fractions

The present article describes the isolation and characterization of secondary metabolites from the leaves of Cerbera odollam as well as bioactivities of the crude methanolic extract and its petroleum ether (PE), carbon tetrachloride (CTC), chloroform (CF) and aqueous (AQ) soluble fractions. In this investigation, successive chromatographic separation and purification yielded β-amyrin (CO-01), lupeol (CO-02), β-sitostenone (CO-03) and triticusterol (CO-04). The study also indicated the antioxidant, cytotoxic, antimicrobial, membrane stabilizing activities and poor thrombolytic activities. Thus, the present study endeavors to provide scientific basis to establish Cerbera odollam as a potential medicinal plant.Dhaka Univ. J. Pharm. Sci. 16(2): 179-186, 2017 (December)

scholarly journals Isolation and characterization of new secondary metabolites from Asphodelus microcarpus

2014 ◽  
Vol 23 (7) ◽  
pp. 3510-3515 ◽  
Author(s):  
Mohammed M. Ghoneim ◽  
Khaled M. Elokely ◽  
Atef A. El-Hela ◽  
Abd Elsalam I. Mohammad ◽  
Melissa Jacob ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Isolation And Characterization ◽  
Asphodelus Microcarpus

Isolation and characterization of culmorin derivatives produced by Fusariumculmorum CMI 14764

1992 ◽  
Vol 70 (5) ◽  
pp. 1308-1316 ◽  
Author(s):  
Gertrude C. Kasitu ◽  
John W. ApSimon ◽  
Barbara A. Blackwell ◽  
David A. Fielder ◽  
Roy Greenhalgh ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Secondary Metabolites ◽  
Liquid Culture ◽  
Farnesyl Pyrophosphate ◽  
Isolation And Characterization ◽  
Derivatives Of

Liquid culture fermentations of Fusariumculmorum yield a wide variety of secondary metabolites. In addition to known isoprenoids, such as the trichothecenes and modified trichothecenes, four new derivatives of culmorin have been isolated and characterized by spectral analysis. These compounds are 15-hydroxyculmorone (7), and 5-hydroxy- (8), 12-hydroxy- (10), and 15-hydroxyculmorin (9). The structure of culmorone is confirmed. The biosynthesis of culmorin from farnesyl pyrophosphate is substantiated through the use of 13C-acetate and the oxidation of these derivatives is discussed.

Isolation and characterization of secondary metabolites fromPlumeria obtusa

2011 ◽  
Vol 13 (12) ◽  
pp. 1122-1127 ◽  
Author(s):  
Muhammad Saleem ◽  
Nasim Akhtar ◽  
Naheed Riaz ◽  
Muhammad Shaiq Ali ◽  
Abdul Jabbar
Keyword(s):  
Secondary Metabolites ◽  
Isolation And Characterization

scholarly journals Preliminary phytochemical profile of Dictyota dichotoma (Huds.) Lamouroux collected from Koothankuzhi Coast, Tirunelveli district, Tamil Nadu, India

2018 ◽  
Vol 8 (6-s) ◽  
pp. 53-56
Author(s):  
Paul J John Peter
Keyword(s):  
Secondary Metabolites ◽  
Tamil Nadu ◽  
Cardiac Glycosides ◽  
East Coast ◽  
Phytochemical Analysis ◽  
Dictyota Dichotoma ◽  
Seaweed Extracts ◽  
Isolation And Characterization ◽  
Phytochemical Profile

The present study was intended to discover the preliminary phytochemicals of Dictyota dichotoma (Huds.) Lamouroux from Koothankuzhi coast, Tirunelveli district, the south east coast of Tamil Nadu, India. The preliminary phytochemical analysis was conducted in seven extracts namely methanol, acetone, chloroform, ethyl acetate, petroleum ether, hexane and benzene by Harborne method. The preliminary phytochemical analysis showed the presence of alkaloids, anthocyanin, anthraquinones, cardiac glycosides, catechin, coumarins, diterpenes, emodins, flavonoids, glycosides, leucoanthocyanin, lignins, phenols, phlobatannins, quinones, saponins, steroids, tannins, terpenoids and triterpenoids. Among the various phytochemicals studied, tannin showed the maximum presence, being found in seven different extracts and anthraquinones was observed in only one extract. From the results, it was noted that the extracts of Dictyota dichotoma (Huds.) Lamouroux was found to be the presence of a number of active secondary metabolites. This report will lead to the isolation and characterization of these active secondary metabolites for bioefficacy and bioactivity. Keywords: Phytochemical, Bioactive compounds, Seaweed extracts, Dictyota, Tamil Nadu.

scholarly journals Evaluation of endophytic fungal fractions of Andrographis paniculata (Burm.f.) Wall. Nees leaves for in vitro free radical scavenging and hepatoprotective activity

2018 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Smita Kishor Puri ◽  
Prasanna Vasantrao Habbu ◽  
Preeti Venkatrao Kulkarni ◽  
Venkatrao Hanumanthrao Kulkarni
Keyword(s):  
Free Radical ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Andrographis Paniculata ◽  
Rdna Sequencing

Fungal endophytes are the microbes residing in internal tissues of the plant forming symbiotic, mutualistic, communalistic and trophobiotic relationship. Endophytes from medicinal plants are considered as essential source of secondary metabolites accompanied by interesting biological/pharmacological activities. In this study, an effort was made to isolate, characterize endophytic fungi from leaves of Andrographis paniculata and to screen the fungal fractions for in-vitro antioxidant and hepatoprotective activity. Two fungal endophytes (APLF-1 and APLF-2) from Andrographis paniculata were isolated and fermented to get chloroform (A1C, A2C), ethyl acetate (A1EA, A2EA) and n butanol (A1nB, A2nB) extracts. All the endophytic fractions of APLF-1 and APLF-2 were assayed for free radical scavenging properties against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and reducing power. Fractions of APLF-2 showed good scavenging activity compared to APLF-1 extracts. Further, A2EA and A2nB were evaluated for hepatoprotective activity against CCl4 induced hepatotoxicity. A2EA (50 mg/kg &100 mg/kg) and A2nB (50 mg/kg &100 mg/kg) reversed the elevated biochemical parameters with respect to CCl4 treated group (p<0.001). The LPO, SOD and CAT levels were also restored by A2EA and A2nB (100 mg/kg p.o). APLF-1 and APLF-2 were studied for rDNA sequencing by PCR technique. The endophytic fungi, APLF-1 and APLF-2 were identified as Diaporthe sp. A25 and Preussia sp. PPV3.6 respectively based on their morphology and molecular characterization. The presence of polyvalent secondary metabolites in A2EA and A2nB were confirmed by HPTLC analysis. Keyword: Endophytic fungi; Hepatoprotective; Antioxidant; Andrographis paniculata; Diaporthe sp. A25; Preussia sp. PPV3.6

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