Gene dunce localization in the polytene chromosome of Drosophila melanogaster long span batch of adjacent chromosomal structures

The molecular and chromosomal localization of the dunce gene was studied. This gene (167.3 kb) consists almost entirely of introns, in which a cluster of seven short tissue-specific genes is located. On the basis of the results of FISH analysis of the gene fragments, we established that the dunce gene is located within nine chromosomal structures (four bands and five interbands), which contradicts the common idea that genes are located in only one structure (band or interband) or at the boundary of these structures. Our results are quite unexpected and original and greatly expand the current understanding of the genetic organization of interphase chromosomes.

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Gene dunce Localization in the Polytene Chromosome of Drosophila melanogaster Long Span Batch of Adjacent Chromosomal Structures

Doklady Biochemistry and Biophysics ◽

10.1134/s1607672919010137 ◽

2019 ◽

Vol 484 (1) ◽

pp. 55-58 ◽

Cited By ~ 1

Author(s):

V. A. Khoroshko ◽

G. V. Pokholkova ◽

T. Yu. Zykova ◽

I. S. Osadchiy ◽

I. F. Zhimulev

Keyword(s):

Drosophila Melanogaster ◽

Polytene Chromosome ◽

Long Span

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CYTOGENETIC ANALYSIS OF CHROMOSOME 3 IN DROSOPHILA MELANOGASTER: THE HOMOEOTIC GENE COMPLEX IN POLYTENE CHROMOSOME INTERVAL 84A-B

Genetics ◽

10.1093/genetics/94.1.115 ◽

1980 ◽

Vol 94 (1) ◽

pp. 115-133 ◽

Cited By ~ 18

Author(s):

Thomas C Kaufman ◽

Ricki Lewis ◽

Barbara Wakimoto

Keyword(s):

Drosophila Melanogaster ◽

Polytene Chromosome ◽

Cytogenetic Analysis ◽

Proximal Portion ◽

Chromosome 3 ◽

Gene Complex ◽

Anterior Portion ◽

Cytogenetic Evidence ◽

The Right

ABSTRACT Cytogenetic evidence is presented demonstrating that the 84A-B interval in the proximal portion of the right arm of chromosome 3 is the residence of a homoeotic gene complex similar to the bithorax locus. This complex, originally defined by the Antennapedia (A n t p) mutation, controls segmentation in the anterior portion of the organism. Different lesions within this complex homoeotically transform portions OI the prothorax, proboscis, antenna and eye and present clear analogies to similar lesions within the bithorax locus.

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Contrasting Histories of Three Gene Regions Associated With In(3L)Payne of Drosophila melanogaster

Genetics ◽

10.1093/genetics/144.4.1565 ◽

1996 ◽

Vol 144 (4) ◽

pp. 1565-1575 ◽

Cited By ~ 3

Author(s):

Esteban Hasson ◽

Walter F Eanes

Keyword(s):

Drosophila Melanogaster ◽

Sequence Variation ◽

Present Report ◽

Genetic Exchange ◽

Nucleotide Variation ◽

Distal Breakpoint ◽

Esterase 6 ◽

The Common ◽

Neutrality Tests ◽

Hsp83 Gene

In the present report, we studied nucleotide variation in three gene regions of Drosophila melanogaster, spanning >5 kb and showing different degrees of association with the cosmopolitan inversion In(3-L)Payne. The analysis of sequence variation in the regions surrounding the breakpoints and the heat shock 83 (Hsp83) gene locus, located close to the distal breakpoint, revealed the absence of shared polymorphisms and the presence of a number of fixed differences between arrangements, indicating absence of genetic exchange. In contrast, for the esterase-6 gene region, located in the center of the inversion, we observed the presence of shared polymorphisms between arrangements suggesting genetic exchange. In the regions close to the breakpoints, the common St arrangement is 10 times more polymorphic than inverted chromosomes. We propose that the lack of recombination between arrangements in these regions coupled with genetic hitchhiking is the best explanation for the low heterozygosity observed in inverted lines. Using the data for the breakpoints, we estimate that this inversion polymorphism is around 0.36 million yr old. Although it is widely accepted that inversions are examples of balanced polymorphisms, none of the current neutrality tests including our Monte Carlo simulations showed significant departure from neutral expectations.

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Molecular cloning and chromosomal localization of a sarco/endoplasmic reticulum-type Ca2+-ATPase of drosophila melanogaster

Biochemical and Biophysical Research Communications ◽

10.1016/s0006-291x(05)80867-8 ◽

1990 ◽

Vol 173 (3) ◽

pp. 872-877 ◽

Cited By ~ 30

Author(s):

Attila Magyar ◽

András Váradi

Keyword(s):

Endoplasmic Reticulum ◽

Drosophila Melanogaster ◽

Molecular Cloning ◽

Chromosomal Localization

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Studies on Genetic Organization in Higher Organisms, II. Complementation and Fine Structure of the Maroon-like Locus of Drosophila melanogaster

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.65.4.939 ◽

1970 ◽

Vol 65 (4) ◽

pp. 939-946 ◽

Cited By ~ 26

Author(s):

V. G. Finnerty ◽

P. Duck ◽

A. Chovnick

Keyword(s):

Drosophila Melanogaster ◽

Fine Structure ◽

Genetic Organization

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Sex and tissue‐specific evolution of developmental plasticity in Drosophila melanogaster

Ecology and Evolution ◽

10.1002/ece3.7136 ◽

2020 ◽

Author(s):

Didem P. Sarikaya ◽

Katherine Rickelton ◽

Julie M. Cridland ◽

Ryan Hatmaker ◽

Hayley K. Sheehy ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Developmental Plasticity ◽

Tissue Specific

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Three-dimensional organization of Drosophila melanogaster interphase nuclei. I. Tissue-specific aspects of polytene nuclear architecture.

The Journal of Cell Biology ◽

10.1083/jcb.104.6.1455 ◽

1987 ◽

Vol 104 (6) ◽

pp. 1455-1470 ◽

Cited By ~ 78

Author(s):

M Hochstrasser ◽

J W Sedat

Keyword(s):

Drosophila Melanogaster ◽

Three Dimensional ◽

Nuclear Architecture ◽

Close Packing ◽

Prothoracic Gland ◽

Cell Type ◽

Interphase Chromosome ◽

Tissue Specific ◽

Spatial Domains ◽

Four Levels

Interphase chromosome organization in four different Drosophila melanogaster tissues, covering three to four levels of polyteny, has been analyzed. The results are based primarily on three-dimensional reconstructions from unfixed tissues using a computer-based data collection and modeling system. A characteristic organization of chromosomes in each cell type is observed, independent of polyteny, with some packing motifs common to several or all tissues and others tissue-specific. All chromosomes display a right-handed coiling chirality, despite large differences in size and degree of coiling. Conversely, in each cell type, the heterochromatic centromeric regions have a unique structure, tendency to associate, and intranuclear location. The organization of condensed nucleolar chromatin is also tissue-specific. The tightly coiled prothoracic gland chromosomes are arrayed in a similar fashion to the much larger salivary gland chromosomes described previously, having polarized orientations, nonintertwined spatial domains, and close packing of the arms of each autosome, whereas hindgut and especially the unusually straight midgut chromosomes display striking departures from these regularities. Surprisingly, gut chromosomes often appear to be broken in the centric heterochromatin. Severe deformations of midgut nuclei observed during gut contractions in living larvae may account for their unusual properties. Finally, morphometric measurements of chromosome and nuclear dimensions provide insights into chromosome growth and substructure and also suggest an unexpected parallel with diploid chromatin organization.

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Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster

PLoS ONE ◽

10.1371/journal.pone.0018853 ◽

2011 ◽

Vol 6 (4) ◽

pp. e18853 ◽

Cited By ~ 17

Author(s):

Ying Chen ◽

Hongzheng Dai ◽

Sidi Chen ◽

Luoying Zhang ◽

Manyuan Long

Keyword(s):

Drosophila Melanogaster ◽

Male Courtship ◽

Specific Expression ◽

Tissue Specific ◽

Tissue Specific Expression

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CYTOGENETIC ANALYSIS OF THE CHROMOSOMAL REGION IMMEDIATELY ADJACENT TO THE ROSY LOCUS IN DROSOPHILA MELANOGASTER

Genetics ◽

10.1093/genetics/95.1.95 ◽

1980 ◽

Vol 95 (1) ◽

pp. 95-110 ◽

Cited By ~ 3

Author(s):

Arthur J Hilliker ◽

Stephen H Clark ◽

Arthur Chovnick ◽

William M Gelbart

Keyword(s):

Drosophila Melanogaster ◽

Polytene Chromosome ◽

Structural Information ◽

Genetic Regulation ◽

Chromosome Band ◽

Genetic Dissection ◽

Genetic Unit ◽

Complementation Groups ◽

The Relationship ◽

Chromosome Bands

ABSTRACT This report describes the genetic analysis of a region of the third chromosome of Drosophila melanogaster extending from 87D2-4 to 87E12-F1, an interval of 23 or 24 polytene chromosome bands. This region includes the rosy (ry, 3-52.0) locus, carrying the structural information for xanthine dehydrogenase (XDH). We have, in recent years, focused attention on the genetic regulation of the rosy locus and, therefore, wished to ascertain in detail the immediate genetic environmcnt of this locus. Specifically, we question if rosy is a solitary genetic unit or part of a larger complex genetic unit encompassing adjacent genes. Our data also provide opportunity to examine further the relationship between euchromatic gene distrihution and polytene chromosome structure.—The results of our genetic dissection of the rosy microregion substantiate the conclusion drawn earlier (SCHALET, KERNAGHAN and CHOVNICK 1964) that the rosy locus is the only gene in this region concerned with XDH activity and that all adjacent genetic units are functionally, as well as spatially, distinct Erom the rosy gene. Within the rosy micro-region, we observed a close correspondence between the number of complementation groups (21) and the number of polytene chromosome bands (23 or 24). Consideration of this latter observation in conjunction with those of similar studies of other chhromosomal regions supports the hypothesis that each polytene chromosome band corresponds to a single genetic unit.

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