scholarly journals Virulence Analysis of Wheat Powdery Mildew Fungus Population by Mobile Nurseries.

1992 ◽  
Vol 58 (5) ◽  
pp. 752-756
Author(s):  
Takashi OKU ◽  
Tsuneo TSUCHIZAKI
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Fungus ◽  
Wheat Powdery Mildew ◽  
Virulence Analysis

scholarly journals An important role for secreted esterase in disease establishment of the wheat powdery mildew fungus Blumeria graminis f. sp.tritici

2011 ◽  
Vol 57 (3) ◽  
pp. 211-216 ◽  
Author(s):  
Jie Feng ◽  
Feng Wang ◽  
Geoff R. Hughes ◽  
Susan Kaminskyj ◽  
Yangdou Wei
Keyword(s):  
Powdery Mildew ◽  
Blumeria Graminis ◽  
Powdery Mildew Fungus ◽  
Diisopropyl Fluorophosphate ◽  
Wheat Powdery Mildew ◽  
Leaf Surfaces ◽  
Indigo Blue ◽  
Serine Esterases ◽  
Wheat Leaves

The activity of esterase secreted by conidia of wheat powdery mildew fungus, Blumeria graminis f. sp. tritici, was assayed using indoxyl acetate hydrolysis, which generates indigo blue crystals. Mature, ungerminated, and germinating conidia secrete esterase(s) on artificial media and on plant leaf surfaces. The activity of these esterases was inhibited by diisopropyl fluorophosphate, which is selective for serine esterases. When conidia were inoculated on wheat leaves pretreated with diisopropyl fluorophosphate, both appressorial germ tube differentiation and symptom development were significantly impaired, indicating an important role of secreted serine esterases in wheat powdery mildew disease establishment.

scholarly journals Virulence Analysis of Wheat Powdery Mildew (Blumeria graminis f. sp. tritici) and Effective Genes in Middle Delta, Egypt

Plant Disease ◽  
2016 ◽  
Vol 100 (9) ◽  
pp. 1927-1930 ◽  
Author(s):  
Moustafa M. El-Shamy ◽  
Hassan M. Emara ◽  
Mona E. Mohamed
Keyword(s):  
Powdery Mildew ◽  
Adult Stage ◽  
Resistance Breeding ◽  
Blumeria Graminis ◽  
Wheat Cultivars ◽  
Natural Field ◽  
Wheat Powdery Mildew ◽  
Virulence Analysis ◽  
High Virulence ◽  
First Time

Wheat powdery mildew (Blumeria graminis f. sp. tritici) has increased in Delta Egypt in recent years as a result of growing susceptible cultivars of bread wheat. Fifty-two isolates of B. graminis f. sp. tritici collected in 2013 and 2014 from commercial fields in five provinces were analyzed for virulence, using 21 genes conferring resistance to powdery mildew (Pm genes) as well as 12 local wheat cultivars. First true leaves of 10-day-old wheat differentials were inoculated with isolates derived from a single pustule of B. graminis f. sp. tritici. No virulence to the resistance genes Pm3d, Pm12, Pm16, Pm24, Pm35, Pm36, and Pm37 was detected, indicating that these genes could be used for resistance breeding. Virulence to Pm2, Pm4a, and Pm6 was low, while high virulence to genes Pm1a, Pm3a, Pm3c, Pm3f, Pm5a, Pm7, Pm8, Pm9, and Pm17 was found, indicating that breeders should be very cautious in using these genes. At the adult stage, genes Pm2, Pm3d, Pm3f, Pm4a, Pm6, Pm12, Pm16, Pm17, Pm24, Pm35, Pm36, and Pm37 were completely effective in both years. All the tested Egyptian wheat cultivars were susceptible under natural field conditions. The virulence of B. graminis f. sp. tritici population and resistance Pm genes have been assessed for the first time in Egypt.

Studies on the control effect of Bacillus subtilis on wheat powdery mildew

2021 ◽  
Author(s):  
Deshan Xie ◽  
Xuewei Cai ◽  
Chunping Yang ◽  
Linjun Xie ◽  
Guangwei Qin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Powdery Mildew ◽  
Control Effect ◽  
Wheat Powdery Mildew

scholarly journals Disruption of barley immunity to powdery mildew by an in-frame Lys-Leu deletion in the essential protein SGT1

Genetics ◽  
2020 ◽  
Vol 217 (2) ◽  
Author(s):  
Antony V E Chapman ◽  
Matthew Hunt ◽  
Priyanka Surana ◽  
Valeria Velásquez-Zapata ◽  
Weihui Xu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Fungal Pathogens ◽  
Novel Mutation ◽  
Exome Capture ◽  
Resistance Locus ◽  
Hordeum Vulgare L ◽  
Powdery Mildew Fungus ◽  
Pathogen Effectors ◽  
Cell Processes ◽  
Disease Resistances

Abstract Barley (Hordeum vulgare L.) Mla (Mildew resistance locus a) and its nucleotide-binding, leucine-rich-repeat receptor (NLR) orthologs protect many cereal crops from diseases caused by fungal pathogens. However, large segments of the Mla pathway and its mechanisms remain unknown. To further characterize the molecular interactions required for NLR-based immunity, we used fast-neutron mutagenesis to screen for plants compromised in MLA-mediated response to the powdery mildew fungus, Blumeria graminis f. sp. hordei. One variant, m11526, contained a novel mutation, designated rar3 (required for Mla6 resistance3), that abolishes race-specific resistance conditioned by the Mla6, Mla7, and Mla12 alleles, but does not compromise immunity mediated by Mla1, Mla9, Mla10, and Mla13. This is analogous to, but unique from, the differential requirement of Mla alleles for the co-chaperone Rar1 (required for Mla12 resistance1). We used bulked-segregant-exome capture and fine mapping to delineate the causal mutation to an in-frame Lys-Leu deletion within the SGS domain of SGT1 (Suppressor of G-two allele of Skp1, Sgt1ΔKL308–309), the structural region that interacts with MLA proteins. In nature, mutations to Sgt1 usually cause lethal phenotypes, but here we pinpoint a unique modification that delineates its requirement for some disease resistances, while unaffecting others as well as normal cell processes. Moreover, the data indicate that the requirement of SGT1 for resistance signaling by NLRs can be delimited to single sites on the protein. Further study could distinguish the regions by which pathogen effectors and host proteins interact with SGT1, facilitating precise editing of effector incompatible variants.

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