scholarly journals UJI ANTIOKSIDAN EKSTRAK HEKSANA, ETIL ASETAT, ETANOL, METANOL 80% DAN AIR DAUN KELOR (Moringa oleifera, Lamk)

2017 ◽  
Vol 1 (1) ◽  
pp. 40
Author(s):  
Agung Abadi Kiswandono ◽  
Mamay Maslahat

Antioxidant Test of  Hexane, Etil Asetat, Ethanol, Methanol 80% and   Water Extract in Moringa oleifera           Antioxidant is a substance that can protect a materials from its destruction. Sources of antioxidant could be from plants. Alkaloid compounds in the Moringa oleifera leaves have  potential  antioxidant source. This research aimed  to examine antioxidant potential from Moringa oleifera leaves extract of hexane, ethyl acetate, methanol 80% and water .   The extraction methodes was meseration, and antioxidant test used the DPPH free radical scavenging effect. The  result showed that extract ethanol had the best potential antioxidant with the value of 118.19 µg/mL and R2 was 99.9%, from hexanes was 692.39 µg/mL (R2=99.9%), in ethyl acetate was 247.5 µg/mL (R2=99.9%), in methanol 80% was  121.79 µg/mL (R2= 0.998) and in water extract 189.21 µg/mL (R2=99.8%). Antioxidant standart as kuersetin that gave value of 15.84 µg/mL (R2 = 99.9%).Keywords : Bioactives compound, moringa oleifera,  antioxidant ABSTRAK          Antioksidan merupakan substansi yang dapat mencegah atau memperlambat kerusakan suatu zat. Sumber antioksidan dapat diperoleh dari suatu tumbuhan. Senyawa alkaloid yang terdapat pada daun kelor berpotensi sebagai sumber antioksidan. Penelitian ini  bertujuan menguji antioksidan ekstrak heksana, etil asetat, etanol, metanol 80% dan air pada daun kelor (Moringa oleifera, Lamk). Penelitian dilakukan dengan menggunakan metode maserasi dan uji antioksidan menggunakan metode ”DPPH free radical scavenging effect”. Hasil uji antioksidan terbaik terdapat pada ekstrak etanol, yaitu sebesar 118.19 µg/mL dengan harga R2 sebesar 99.9%, sedangkan pelarut yang lain, yaitu pelarut heksana sebesar 692.39 µg/mL (R2=99.9%), etil asetat 247.5 µg/mL (R2=99.9%), metanol 80% 121.79 µg/mL (R2= 0.998) dan air 189.21 µg/mL (R2=99.8%), sebagai pembanding antioksidan digunakan kuersetin, yaitu 15.84 µg/mL (R2= 99.9%).Kata kunci : Senyawa bioaktif, moringa oleifera,  antioksidan

2015 ◽  
Vol 35 ◽  
pp. 6-12 ◽  
Author(s):  
Thiyagarajan Rajeshwari ◽  
Boobalan Raja

This study was planned to assess the antioxidant and free radical scavenging effect of D-carvone against L-NAME (Nω-nitro-L-arginine methyl ester hydrochloride) induced hypertension. Hypertension was encouraged in adult male albino rats of the Wistar strain, considering 180–230 g, by oral administration of the L-NAME (40 mg/kg/ body weight/day) in drinking water for 4 weeks. Rats were cured with D-carvone (5, 10 and 20 mg/kg body weight) for four weeks. A significant reduction in the levels of non-enzymatic antioxidants such as vitamin C, vitamin E and reduced glutathione (GSH), in plasma were perceived in L-NAME induced hypertensive rats. Moreover, in vitro free radical scavenging activity of ABTS+ and DPPH• radical scavenging possible of D-carvone was also quantified. Treatment with D-carvone (5, 10 and 20 mg/kg bw) carries back all the above parameters to near usual level, in which 20 mg/kg displayed the highest effect than that of other two doses. Further, D-carvone displays concentration dependent antioxidant potential. These results suggest that D-carvone acts as an antioxidant and free radical scavenging agent against L-NAME induced hypertension.


2011 ◽  
Vol 6 (3) ◽  
pp. 218-222 ◽  
Author(s):  
P. Thirunavuk ◽  
T. Ramanathan ◽  
R. Shanmugapr ◽  
G. Umamaheswa ◽  
G. Renugadevi

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