scholarly journals Identifikasi Molekular Bakteri Glukanolitik Indigenous KE-B6 dari Saluran Pencernaan Bekicot (Achatina fulica)

2019 ◽  
Vol 11 (2) ◽  
pp. 64-71
Author(s):  
Wijanarka Wijanarka ◽  
Sri Pujiyanto ◽  
Budi Raharjo
Keyword(s):  
Serratia Marcescens ◽  
Digestive Tract ◽  
Bacterial Growth ◽  
Sequence Data ◽  
Bacterial Isolates ◽  
Achatina Fulica ◽  
Way Of Life ◽  
16S Rdna Sequence ◽  
Rdna Sequence Data ◽  
Key Words Bacteria

Glucanolitic bacteria are bacteria that have the ability to break down glucan into glucose monomer units. The ability of the bacteria is caused by the presence of glucanase enzymes. The choice of glucanase derived from bacteria is based on the ability and speed of bacterial growth in terms of producing glucanase enzymes. The presence of bacteria and protozoa in the digestive tract symbiotic with each other to digest cellulose or concomitant materials . Based on the ability of the way of life to digest forage and leaf litter, it is suspected that snails (Achatina fulica) have the ability to produce glucanase biocatalysts, especially in the digestive tract. To find out the characteristics and characteristics of indigenous bacteria snail canals, identification of KE-B6 bacteria is carried out molecularly so that accurate and accurate results are obtained. The Basic Alignment Search Tools BLAST results of KE-B6 bacterial isolates based on 16S rDNA sequence data with 27F (Forward) and 1492R (Reverse) primers showed that these bacterial isolates had homology of 99.64% to Serratia marcescens.  Key words: bacteria, glucanolitic, A. fulica, Serratia marcescens.

Anterastes davrazensis sp. n. (Orthoptera, Tettigoniidae): morphology, song and 16S rDNA phylogeny

Zootaxa ◽  
2012 ◽  
Vol 3401 (1) ◽  
pp. 49 ◽  
Author(s):  
SARP KAYA ◽  
DRAGAN CHOBANOV ◽  
BATTAL ÇIPLAK
Keyword(s):  
New Species ◽  
16S Rdna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
16S Rdna Sequence ◽  
Rdna Sequence Data ◽  
Male Calling ◽  
Relationship Of ◽  
The Relationship

The new species Anterastes davrazensis sp. n. (Orthoptera, Tettigoniidae) is described from south-eastern Turkey. Description, diagnosis and relationships of the new species were studied utilizing morphology, male calling songs and 16S rDNA sequence data from all species in the genus. Morphology and song syllable structure indicate A. davrazensis sp. n. is related to A. uludaghensis. Phylogenetic analyses based on representative haplotypes of 16S rDNA, using Sureyaella bella, Parapholidoptera distincta and Bolua turkiyae as outgroups, also suggested strong support to the relationship of these two species. A. davrazensis sp. n. differs from its closest relative A. uludaghensis by the higher number of stridulatory pegs and the song, consisting of irregular syllable groups.

scholarly journals Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity

2016 ◽  
Vol 8 (3) ◽  
pp. 386
Author(s):  
Wijanarka Wijanarka ◽  
Endang Kusdiyantini ◽  
Sarjana Parman
Keyword(s):  
Enzyme Activity ◽  
Digestive Tract ◽  
Generation Time ◽  
Cellulase Activity ◽  
Biology Education ◽  
Activity Measurement ◽  
Bacterial Isolates ◽  
Achatina Fulica ◽  
Cellulolytic Bacteria ◽  
Activity Measurements

<p>On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (<em>Achatina fulica</em>). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate (µ) 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).</p><p><strong>How to Cite</strong></p><p>Wijanarka, W., Kusdiyantini, E. &amp; Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (<em>Achatina fulica</em>) and Test the Ability of Cellulase Activity. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 386-392. </p>

Activated sludge foaming: the true extent of actinomycete diversity

1998 ◽  
Vol 37 (4-5) ◽  
pp. 511-519 ◽  
Author(s):  
Michael Goodfellow ◽  
Fiona M. Stainsby ◽  
Russell Davenport ◽  
Jongsik Chun ◽  
Tom Curtis
Keyword(s):  
Mass Spectrometry ◽  
Activated Sludge ◽  
Sequence Data ◽  
Novel Species ◽  
Chemical Properties ◽  
Pyrolysis Mass Spectrometry ◽  
16S Rdna Sequence ◽  
Rdna Sequence Data ◽  
Actinomycete Diversity ◽  
Curie Point Pyrolysis

Isolates from activated sludge foam were provisionally assigned to the genera Gordona and Tsukamurella on the basis of colony morphology and pigmentation. Representatives of the first group were compared with marker strains of validly described species of Gordona by using Curie-point pyrolysis mass spectrometry. Most of the isolates fell into a number of taxa which were equated with groups of marker strains which corresponded to the known Gordona species. In a corresponding experiment the activated sludge isolates, which were provisionally labelled “Tsukamurella spumae” formed a group which was well separated from the marker strains of the genus. Representatives of the isolates were found to have chemical properties consistent with their classification in the genus Tsukamurella. 16S rDNA sequence data, when taken with previous DNA:DNA relatedness results, suggest that Tsukamurella paurometabola is heterogeneous. Similarly, the sequence data provide some evidence that “Tsukamurella spumae” may merit recognition as a novel species.

Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti

2005 ◽  
Vol 107 (1-2) ◽  
pp. 63-69 ◽  
Author(s):  
Janet E. Hill ◽  
Marcelo Gottschalk ◽  
Roland Brousseau ◽  
Josée Harel ◽  
Sean M. Hemmingsen ◽  
...  
Keyword(s):  
16S Rdna ◽  
Biochemical Analysis ◽  
Sequence Data ◽  
Streptococcus Suis ◽  
Rdna Sequence ◽  
16S Rdna Sequence ◽  
Rdna Sequence Data

Development and Evaluation of 16S rDNA Microarray for Detecting Bacterial Pathogens in Cerebrospinal Fluid

2005 ◽  
Vol 230 (8) ◽  
pp. 587-591 ◽  
Author(s):  
Yi Liu ◽  
Jin-Xiang Han ◽  
Hai-Yan Huang ◽  
Bo Zhu
Keyword(s):  
Cerebrospinal Fluid ◽  
16S Rdna ◽  
Culture Media ◽  
Sequence Data ◽  
Rapid Identification ◽  
Universal Primers ◽  
16S Rdna Sequence ◽  
Rdna Sequence Data ◽  
Identification Of Bacteria ◽  
Culture Positive

The rapid identification of bacteria in cerebrospinal fluid (CSF) is very Important for patient management and antimicrobial therapies. We developed a 16S DNA microarray–based method that targets 16S rDNA and can directly detect bacteria from CSF without cultivation. Universal primers and specific probes were designed from the 16S rDNA sequence data retrieved directly from the GenBank database. The specificity of the assay is obtained through a combination of microarray hybridization and enzymatic labeling of the constructed specific probes. Cultivation-dependent assays were used as reference methods in the development and evaluation of the method. With the exception of Mycobacterium tuberculosis and Proteus mirabilis, forty-five positive blood culture media were successfully differentiated. When this procedure was applied directly to 100 CSF specimens, 29 specimens from 16 patients were positive by bacterial culture and 3 culture-positive CSF specimens produced no hybridized signals. The remaining 26 specimens were correctly identified, including one with mixed infection. The accuracy, sensitivity, and specificity of the assay can be increased further by designing more oligonucleotides for the microarray. This method is versatile and makes it possible to detect more bacteria in a single assay and discriminate different bacterial genera.

Sign in / Sign up

Export Citation Format

Share Document