MGB-based one-step multiplex real-time PCR method for rapid detection of HPV

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

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A One-Step, Real-Time PCR Assay for Rapid Detection of Rhinovirus

Journal of Molecular Diagnostics ◽

10.2353/jmoldx.2010.090071 ◽

2010 ◽

Vol 12 (1) ◽

pp. 102-108 ◽

Cited By ~ 16

Author(s):

Duc H. Do ◽

Stella Laus ◽

Amy Leber ◽

Mario J. Marcon ◽

Jeanne A. Jordan ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Pcr Assay ◽

One Step

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Rapid detection of mutations related to Mycobacterium leprae drug resistance by using Hp-rPCR (hairpin primer- real time PCR) method

Japanese journal of leprosy ◽

10.5025/hansen.83.6 ◽

2014 ◽

Vol 83 (1) ◽

pp. 6-13

Author(s):

Masanori Kai

Keyword(s):

Drug Resistance ◽

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Mycobacterium Leprae ◽

Pcr Method ◽

Detection Of Mutations

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Development of a sybr green real-time pcr method for rapid detection ofred sea bream iridovirus

Environmental Science and Biological Engineering ◽

10.2495/esbe140501 ◽

2014 ◽

Author(s):

B. Wu ◽

X. Wan ◽

M.Y. Sun ◽

F. Qu

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Sybr Green ◽

Sea Bream ◽

Pcr Method

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Multiplex one-step Real-time PCR by Taqman-MGB method for rapid detection of pan and H5 subtype avian influenza viruses

PLoS ONE ◽

10.1371/journal.pone.0178634 ◽

2017 ◽

Vol 12 (6) ◽

pp. e0178634 ◽

Cited By ~ 7

Author(s):

Zhujun Zhang ◽

Dong Liu ◽

Wenqiang Sun ◽

Jing Liu ◽

Lihong He ◽

...

Keyword(s):

Avian Influenza ◽

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Influenza Viruses ◽

Avian Influenza Viruses ◽

One Step ◽

H5 Subtype

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Rapid Detection of Salmonella in Foods Using Real-Time PCR

Journal of Food Protection ◽

10.4315/0362-028x-71.12.2436 ◽

2008 ◽

Vol 71 (12) ◽

pp. 2436-2441 ◽

Cited By ~ 77

Author(s):

CHORNG-MING CHENG ◽

WEN LIN ◽

KHANH THIEN VAN ◽

LIEUCHI PHAN ◽

NELLY N. TRAN ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Food Samples ◽

Taqman Probe ◽

Cell Culturing ◽

Chili Powder ◽

Pcr Method ◽

Food Matrices ◽

Salmonella Serovars

Conventional methods for detection of Salmonella serovars in foods are generally time-consuming and labor intensive. A real-time PCR method has been developed with custom designed primers and a TaqMan probe to detect the presence of a 262-bp fragment of the Salmonella-specific invA gene. The method has been tested with a total of 384 field-isolated Salmonella serovars and non-Salmonella stock strains, as well as 420 U.S. Food and Drug Administration food samples, comprising a variety of food matrices. The method was highly specific in detecting Salmonella in spiked chili powder and shrimp samples, with a sensitivity of 0.04 CFU/g. In addition, the method is faster, more accurate, and less costly than the traditional U.S. Food and Drug Administration's Bacteriological Analytical Manual cell-culturing and the AOAC International–approved VIDAS methods to detect Salmonella in foods.

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