In-house validation of a real-time PCR method for rapid detection of Salmonella ssp. in food products

2004 ◽  
Vol 219 (2) ◽  
Author(s):  
Dietrich M�de ◽  
Roger Petersen ◽  
Klaus Tr�mper ◽  
Regine Stark ◽  
Lutz Grohmann
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Products ◽  
Pcr Method

scholarly journals Detection of Peanut Allergen Traces with a Real Time PCR Assay - The Challenge to Protect Food-Allergic Consumers

2017 ◽  
Vol 7 (1) ◽  
pp. 32 ◽  
Author(s):  
Dimitra Houhoula ◽  
Stamatios Koussissis ◽  
Vladimiros Lougovois ◽  
John Tsaknis ◽  
Dimitra Kassavita ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Products ◽  
Molecular Techniques ◽  
Food Allergens ◽  
Pcr Assay ◽  
Peanut Allergen ◽  
Pcr Method ◽  
Detection And Quantification

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

MGB-based one-step multiplex real-time PCR method for rapid detection of HPV

2013 ◽  
Vol 12 (3) ◽  
pp. 107-113 ◽  
Author(s):  
Jie Huang ◽  
Chun Gao ◽  
Xilai Ding ◽  
Shoufang Qu ◽  
Licheng Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
One Step ◽  
Pcr Method

scholarly journals A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 286 ◽  
Author(s):  
Amaya Velasco ◽  
Graciela Ramilo-Fernández ◽  
Carmen G. Sotelo
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Food Products ◽  
Polymerase Chain Reaction Method ◽  
Cross Reactivity ◽  
Sepia Officinalis ◽  
Base Pairs ◽  
Mitochondrial Coi ◽  
Fresh Frozen ◽  
Pcr Method

Cephalopods are very relevant food resources. The common cuttlefish (Sepia officinalis) is highly appreciated by consumers and there is a lack of rapid methods for its authentication in food products. We introduce a new minor groove binding (MGB) TaqMan real-time PCR (Polymerase Chain Reaction) method for the authentication of S. officinalis in food products to amplify a 122 base pairs (bp) fragment of the mitochondrial COI (Cytochrome Oxidase I) region. Reference and commercial samples of S. officinalis showed a threshold cycle (Ct) mean of 14.40, while the rest of the species examined did not amplify, or showed a significantly different Ct (p < 0.001). The calculated efficiency of the system was 101%, and the minimum DNA quantity detected was 10−4 ng. No cross-reactivity was detected with any other species, thus, the designed method differentiates S. officinalis from other species of the genus Sepia and other cephalopod species and works for fresh, frozen, grilled, cooked and canned samples of Sepia spp. The method has proved to be reliable and rapid, and it may prove to be a useful tool for the control of fraud in cuttlefish products.

Detection of Sesame Seed DNA in Foods Using Real-Time PCR

2007 ◽  
Vol 70 (4) ◽  
pp. 1033-1036 ◽  
Author(s):  
JENNIFER L. BRZEZINSKI
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sesame Seed ◽  
Food Products ◽  
Taqman Probe ◽  
2S Albumin ◽  
Sesame Seeds ◽  
Baked Goods ◽  
Pcr Method ◽  
Seed Dna

The detection of potentially allergenic foods, such as sesame seeds, in food products is a major concern for the food-processing industry. A real-time PCR method was designed to determine if sesame seed DNA is present in food products. The PCR reaction amplifies a 66-bp fragment of the sesame seed 2S albumin gene, which is detected with a sesame-specific, dual-labeled TaqMan probe. This reaction will not amplify DNA derived from other seeds present in baked goods, such as pumpkin, poppy, and sunflower seeds. Additionally, this assay will not cross-react with DNA from several tree nut species, such as almond, Brazil nut, cashew, hazelnut, and walnut, as well as four varieties of peanut. This assay is sensitive enough to detect 5 pg of purified sesame seed DNA, as well as sesame seed DNA in a spiked wheat cracker sample.

scholarly journals Evaluation of a Real Time PCR Assay Method for the Detection of Genetically Modified Organisms in Food Products

2020 ◽  
Vol 9 (2) ◽  
pp. 1
Author(s):  
Eleni Spanea ◽  
Theofania Tsironi ◽  
Efstathia Tsakali ◽  
Anthimia Batrinou ◽  
Valentina Stefanou ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Food Products ◽  
Assay Method ◽  
Genetically Modified Maize ◽  
Nos Terminator ◽  
Repeatability And Reproducibility ◽  
Pcr Method

The objective of the study was to determine qualitatively by validated Real Time PCR method the occurrence of genetically modified maize and soybean in commercial food products from the Greek market. 70 independent samples were collected, including products from different categories (i.e. cereal based, biscuits and snacks) which declared either corn or soybean on the labelling. The result of the study indicated that 37.1% of maize and soy products (n=70) displayed in the Greek market have detectable levels of genetically modified maize or soy. These products were identified by specific primers and included common GM&Omicron; detection primers for 35S and NOS terminator. Adequate repeatability and reproducibility was demonstrated for the applied Real Time PCR method, as evaluated by intra- and inter-laboratory tests.

Chestnut allergen detection in complex food products: Development and validation of a real-time PCR method

LWT ◽  
2020 ◽  
Vol 123 ◽  
pp. 109067 ◽  
Author(s):  
África Sanchiz ◽  
Isabel Ballesteros ◽  
Adrián López-García ◽  
Ana Ramírez ◽  
Julia Rueda ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Food Products ◽  
Allergen Detection ◽  
Pcr Method ◽  
Development And Validation

Rapid Detection of Salmonella in Foods Using Real-Time PCR

2008 ◽  
Vol 71 (12) ◽  
pp. 2436-2441 ◽  
Author(s):  
CHORNG-MING CHENG ◽  
WEN LIN ◽  
KHANH THIEN VAN ◽  
LIEUCHI PHAN ◽  
NELLY N. TRAN ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Samples ◽  
Taqman Probe ◽  
Cell Culturing ◽  
Chili Powder ◽  
Pcr Method ◽  
Food Matrices ◽  
Salmonella Serovars

Conventional methods for detection of Salmonella serovars in foods are generally time-consuming and labor intensive. A real-time PCR method has been developed with custom designed primers and a TaqMan probe to detect the presence of a 262-bp fragment of the Salmonella-specific invA gene. The method has been tested with a total of 384 field-isolated Salmonella serovars and non-Salmonella stock strains, as well as 420 U.S. Food and Drug Administration food samples, comprising a variety of food matrices. The method was highly specific in detecting Salmonella in spiked chili powder and shrimp samples, with a sensitivity of 0.04 CFU/g. In addition, the method is faster, more accurate, and less costly than the traditional U.S. Food and Drug Administration's Bacteriological Analytical Manual cell-culturing and the AOAC International–approved VIDAS methods to detect Salmonella in foods.

scholarly journals Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus

2012 ◽  
Vol 9 (1) ◽  
pp. 291 ◽  
Author(s):  
Hong Tian ◽  
Jingyan Wu ◽  
Yan Chen ◽  
Keshan Zhang ◽  
Youjun Shang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Sybr Green ◽  
Pcr Method
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