Characterization of shear stress preventing red blood cells aggregation at the individual cell level: The temperature dependence

Red blood cells (RBCs) are subjected to recurrent changes in shear stress and oxygen tension during blood circulation. The cyclic shear stress has been identified as an important factor that...

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In vitro characterization of engineered red blood cells as viral traps against HIV-1 and SARS-CoV-2

Molecular Therapy — Methods & Clinical Development ◽

10.1016/j.omtm.2021.03.003 ◽

2021 ◽

Vol 21 ◽

pp. 161-170 ◽

Cited By ~ 1

Author(s):

Magnus A.G. Hoffmann ◽

Collin Kieffer ◽

Pamela J. Bjorkman

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

In Vitro Characterization ◽

Hiv 1

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A Micromethod for Determination of Cerebrosides, and of the Individual Phosphatides (Ether-insoluble Phosphatide, Lecithin and Kephalin) in Plasma, Red Blood Cells and Tissues1

Acta Medica Scandinavica ◽

10.1111/j.0954-6820.1938.tb19290.x ◽

2009 ◽

Vol 95 (S89) ◽

pp. 178-181

Author(s):

Esben Kirk

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

The Individual

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Characterization of Microparticles and Exosomes from Red Blood Cells During Storage: Implications for Transfusion Toxicity

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2016.10.527 ◽

2016 ◽

Vol 100 ◽

pp. S193

Author(s):

Joo-Yeun Oh ◽

Xin Xu ◽

Kristopher Genschmer ◽

Ming Zhong ◽

Jindong Li ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells

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Anti-Trypanosoma cruzi activity, cytotoxicity and, chemical characterization of extracts from seeds of Lonchocarpus cultratus

The Journal of Infection in Developing Countries ◽

10.3855/jidc.12669 ◽

2021 ◽

Vol 15 (02) ◽

pp. 270-279

Author(s):

Aline Griebler ◽

Fernanda Weyand Banhuk ◽

Izabela Virginia Staffen ◽

Aline Antunes Maciel Bortoluzzi ◽

Thaís Soprani Ayala ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Red Blood Cells ◽

Blood Cells ◽

Methanolic Extract ◽

Chemical Characterization ◽

Peritoneal Macrophages ◽

Chemical Profile ◽

Murine Macrophages ◽

Human Red Blood Cells

Introduction: Trypanosoma cruzi is the agent of Chagas’ disease and affects approximately 6-8 million people worldwide. The search for new anti-T. cruzi drugs are relevant because only two drugs exist actually. The objective of this study was to investigate the effect of the extracts from the seeds of Lonchocarpus cultratus on T. cruzi, its cytotoxicity as well as to elucidate its chemical profile. Methodology: The characterization of the extracts was done using 1H-RMN. T. cruzi forms were treated with increasing concentrations of the extracts and after, the percentage of inhibition and IC50 or LC50 were calculated. Murine peritoneal macrophages were treated with different concentrations of the extracts to evaluate the cellular viability. The hemotoxicity was accessed by verifying the levels of hemolysis caused by the extracts on human red blood cells. Results: Chalcones isocordoin and lonchocarpin were detected in the dichloromethane extract, and chalcone lonchocarpin was detected in the hexane extract. The dichloromethane extract showed higher activity against all the forms of T. cruzi compared to the other two extracts, but the hexane showed the best selectivity index. The cytotoxicity observed in murine macrophages was confirmed in human erythrocytes, with dichloromethane extract having the highest toxicity. The methanolic extract showed the lowest anti-T. cruzi activity but was nontoxic to peritoneal murine macrophages and red blood cells. Conclusions: L. cultratus extracts have the potential to be explored for the development of new anti-trypanosomal drugs. This study was the first to demonstrate the action of extracts from the genus Lonchocarpus on infecting forms of T. cruzi.

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Age-Dependent Acoustic and Microelastic Properties of Red Blood Cells Determined by Vector Contrast Acoustic Microscopy

Microscopy and Microanalysis ◽

10.1017/s143192761200030x ◽

2012 ◽

Vol 18 (3) ◽

pp. 436-444 ◽

Cited By ~ 7

Author(s):

Esam T. Ahmed Mohamed ◽

Albert E. Kamanyi ◽

Mieczysław Pluta ◽

Wolfgang Grill

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Mass Density ◽

Healthy Person ◽

Ultrasonic Waves ◽

Acoustic Microscopy ◽

Cell Parameters ◽

Different Ages ◽

The Individual

AbstractVariations of the mechanical properties of red blood cells that occur during their life span have long been an intriguing task for investigations. The research presented is based on noninvasive monitoring of red blood cells of different ages performed by scanning acoustic microscopy with magnitude and phase contrast. The characteristic signature of fixed cells from groups of three different ages fractionated according to mass density is obtained from the acoustic microscope images, with the data represented in polar graphs. The analysis of these data enables the determination of averaged values for the velocities of ultrasound propagating in the cells from the different groups ranging from (1,681 ± 16) m s−1in the youngest to (1,986 ± 20) m s−1in the oldest group. The determined bulk modulus varies with age from (3.04 ± 0.05) GPa to (4.34 ± 0.08) GPa. An approach to determine for an age-mixed population of red blood cells, collected from a healthy person, the age of the individual cells and the age dependence of the cell parameters including density, velocity, and attenuation of longitudinal polarized ultrasonic waves traveling in the cells is demonstrated.

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