Comparative evaluation of Plateletworks, Multiplate analyzer and Platelet function analyzer-200 in cardiology patients

2018 ◽  
Vol 70 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Jeeyong Kim ◽  
Chi Hyun Cho ◽  
Bo Kyeung Jung ◽  
Jeonghun Nam ◽  
Hong Seog Seo ◽  
...  
Keyword(s):  
Platelet Function ◽  
Comparative Evaluation ◽  
Function Analyzer ◽  
Platelet Function Analyzer ◽  
Multiplate Analyzer

scholarly journals Adequate Platelet Function Inhibition Confirmed by Two Inductive Agents Predicts Lower Recurrence of Ischemic Stroke/Transient Ischemic Attack

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Lulu Zhang ◽  
Xiaowei Hu ◽  
Juehua Zhu ◽  
Xiuying Cai ◽  
Yan Kong ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Antiplatelet Therapy ◽  
Platelet Function ◽  
Adenosine Diphosphate ◽  
Aggregation Rate ◽  
Function Analyzer ◽  
Ischemic Attack ◽  
Platelet Function Analyzer ◽  
Platelet Functions

Background. The correlation between platelet function and recurrent ischemic stroke or TIA remains uncertain. Objective. To investigate two inductive agents to detect platelet functions and assess associations with recurrent ischemic stroke/TIA. Method. The study included 738 ischemic stroke/TIA patients. On days 0, 3, and 9 after antiplatelet therapy, platelet function tests were determined by maximum aggregation rate (MAR) using a PL-11 platelet function analyzer and phase matching reagents. Two induction agents were used: arachidonic acid (AA) and adenosine diphosphate (ADP). At 3-month follow-up, recurrence of stroke/TIA was recorded. Result. Cut-off values of adequate platelet function inhibition were MARADP < 35% and MARAA < 35%. Data showed that antiplatelet therapy could reduce the maximum aggregation rate. More importantly, adequate platelet function inhibition of either MARADP or MARAA was not associated with the recurrence of stroke/TIA, but adequate platelet function inhibition of not only MARADP but also MARAA predicts lower recurrence (0/121 (0.00%) versus 18/459 (3.92%), P = 0.0188). Conclusion. The platelet function tested by PL-11 demonstrated that adequate inhibition of both MARADP and MARAA could predict lower risk of ischemic stroke/TIA recurrence.

Monitoring Acetylsalicylic Acid Effects with the Platelet Function Analyzer PFA-100

2005 ◽  
Vol 31 (04) ◽  
pp. 411-415 ◽  
Author(s):  
Martin Feuring ◽  
Armin Schultz ◽  
Ralf Losel ◽  
Martin Wehling
Keyword(s):  
Acetylsalicylic Acid ◽  
Platelet Function ◽  
Function Analyzer ◽  
Platelet Function Analyzer

scholarly journals Variables influencing Platelet Function Analyzer-100TM closure times in healthy individuals

2005 ◽  
Vol 130 (5) ◽  
pp. 759-767 ◽  
Author(s):  
Hannelore Haubelt ◽  
Christof Anders ◽  
Anette Vogt ◽  
Petra Hoerdt ◽  
Ulrich Theo Seyfert ◽  
...  
Keyword(s):  
Platelet Function ◽  
Healthy Individuals ◽  
Function Analyzer ◽  
Platelet Function Analyzer

scholarly journals The Role of Platelet Function Analyzer Testing in Cardiac Surgery Transfusion Management

2017 ◽  
Vol 44 (2) ◽  
pp. 106-113 ◽  
Author(s):  
Dejana Bogdanic ◽  
Nenad Karanovic ◽  
Jela Mratinovic-Mikulandra ◽  
Branka Paukovic-Sekulic ◽  
Dijana Brnic ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Platelet Function ◽  
Function Analyzer ◽  
Platelet Function Analyzer

The influence of the haematocrit on primary haemostasis in vitro

2005 ◽  
Vol 94 (12) ◽  
pp. 1213-1218 ◽  
Author(s):  
Marco Eugster ◽  
Walter H. Reinhart
Keyword(s):  
Platelet Function ◽  
Type I Collagen ◽  
Inverse Correlation ◽  
Type I ◽  
Membrane Pore ◽  
Closure Time ◽  
Function Analyzer ◽  
Platelet Function Analyzer

SummaryPrimary haemostasis consists of platelet adhesion to subendothelial collagen, their activation and aggregation and finally the formation of a platelet plug. Erythrocytes are involved in this process because they flow in the center of the vessel and push platelets towards the site of action on the vessel wall and enhance shear forces, which activate platelets. In the platelet function analyzer PFA-100® (Dade Behring, Düdingen, Switzerland), the in vivo situation is simulated in vitro with blood being aspirated at high shear rates (5000s-1) through a capillary into a membrane pore with a diameter of 150 μm coated with type I collagen and either epinephrine or adenosine diphosphate. Aggregating platelets plug the pore and stop the flow, which is measured as the closure time. We analysed the influence of erythrocytes on platelet function analyzer measurements by systematic variation of the haematocrit (20,30,40,and 50%) at constant platelet counts of 289±61 ×103/μl plasma, or 152±30 ×103/μl blood, 96±9 ×103/μl blood and 54±5 ×103/μl blood, respectively. An inverse correlation was found between haematocrit and closure time under all circumstances. A decrease of the platelet count by 50 ×103 /μl could be compensated for by a 10% increase in haematocrit. The haematocrit must, therefore, be taken into consideration for the correct interpretation of PFA-100® measurements. Our data also provide a pathophysiological rationale to reduce the risk of bleeding in patients with thrombocytopenia and anaemia by normalizing the haematocrit with erythrocyte transfusions.

Description of an In Vitro Platelet Function Analyzer-PFA-100™

1995 ◽  
Vol 21 (S 02) ◽  
pp. 106-112 ◽  
Author(s):  
Sourav Kundu ◽  
Eric Heilmann ◽  
Reynaldo Sio ◽  
Carmen Garcia ◽  
Rosa Davidson ◽  
...  
Keyword(s):  
Platelet Function ◽  
Quantitative Measure ◽  
Biologically Active ◽  
High Shear Rates ◽  
Function Analyzer ◽  
Potential Applications ◽  
Time Required ◽  
Platelet Function Analyzer ◽  
Von Willebrand

A new in vitro system for the detection of platelet dysfunction, PFA-100™* has been developed. It provides a quantitative measure of platelet function in anticoagulated whole blood. The system comprises a microprocessor-controlled instrument and a disposable test cartridge containing a biologically active membrane. The instrument aspirates a blood sample under constant vacuum from the sample reservoir through a capillary and a microscopic aperture cut into the membrane. The membrane is coated with collagen and epinephrine or adenosine 5’-diphosphate. The presence of these biochemical stimuli, and the high shear rates generated under the standardized flow conditions, result in platelet attachment, activation, and aggregation, slowly building a stable platelet plug at the aperture. The time required to obtain full occlusion of the aperture is reported as the m“closure time.” We have found that impairment of von Willebrand factor, or inhibition of platelet receptors glycoprotein Ib or IIblIIIa with monoclonal antibodies or peptides, resulted in abnormal closure times. An antifibrinogen antibody, in contrast, failed to show any effect. The test appears to be sensitive to platelet adherence and aggregation abnormalities. The PFA-100™ system has potential applications in routine evaluation of platelet function in the clinical setting because of its accuracy, ease of operation, and rapid turnaround of results. * Under evaluation

Laboratory characterization of obligate carriers of type 3 von Willebrand disease with a potential role for Platelet Function Analyzer (PFA‐200)

2022 ◽  
Author(s):  
Tulasi Geevar ◽  
Rutvi Gautam Dave ◽  
Nitty Skariah Mathews ◽  
Snehil Kumar ◽  
Joy John Mammen ◽  
...  
Keyword(s):  
Platelet Function ◽  
Potential Role ◽  
Von Willebrand Disease ◽  
Laboratory Characterization ◽  
Function Analyzer ◽  
Platelet Function Analyzer ◽  
Type 3 ◽  
Von Willebrand

Lack of association between aspirin responsiveness and seven candidate gene haplotypes in patients with symptomatic vascular disease

2009 ◽  
Vol 101 (01) ◽  
pp. 123-133 ◽  
Author(s):  
Shirley Williams ◽  
Diane Nugent ◽  
Paul Harrison ◽  
Helen Segal ◽  
Anila Syed ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Platelet Function ◽  
Purinergic Receptor ◽  
Blood Platelet ◽  
Function Analyzer ◽  
Prostaglandin H ◽  
Ischemic Attack ◽  
Platelet Function Analyzer ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryWe studied the effect of prophylactic aspirin (ASA) ingestion on platelet function in 463 patients with stroke, transient ischemic attack (TIA) or acute coronary disease (ACD), using the Platelet Function Analyzer-100 (PFA-100). We correlated ASA responsiveness with haplotypes of seven candidate genes, selected for their documented role in platelet function, namely, the genes for integrins α2β1and αIIbβ3 (ITGA2, ITGA2B, and ITGB3), platelet glycoproteins Ibα and VI (GPIBA and GP6), the purinergic receptor P2Y1 (P2RY1), and prostaglandin H synthase 1 (PTGS1 = COX1). Non-responsiveness to ASA was defined as the failure of prior ASA ingestion to prolong the PFA-100 closure time (CT) when blood was perfused through cartridges coated with collagen plus epinephrine (CEPI-CT). ASA non-responsiveness was observed in 114 of 463 patients (24.6 %), but was not associated with haplotypes of any of the seven candidate genes. There was also no association between any haplotypes and the CT when blood was perfused through cartridges coated with collagen plus ADP (CADP-CT). The ASA non-responsive cohort had significantly increased whole blood platelet counts (p = 0.03) and plasma von Willebrand Factor antigen levels (p<0.001), which likely contributes to resistance to the inhibitory effects of ASA in the PFA-100.

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