scholarly journals Glutathione Peroxidase Activity is Altered in Vascular Cognitive Impairment-No Dementia and Is a Potential Marker for Verbal Memory Performance

2021 ◽  
pp. 1-12
Author(s):  
Mehnaz Ahmed ◽  
Nathan Herrmann ◽  
Jinghan Jenny Chen ◽  
Mahwesh Saleem ◽  
Paul I. Oh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cognitive Impairment ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Verbal Memory ◽  
Vascular Cognitive Impairment ◽  
Glutathione Peroxidase Activity ◽  
Compensatory Response ◽  
Potential Marker ◽  
Cognitive Impairment No Dementia

Background: Coronary artery disease (CAD) increases risk for vascular cognitive impairment-no dementia (VCIND), a precursor to dementia, potentially through persistent oxidative stress. Objective: This study assessed peripheral glutathione peroxidase activity (GPX), which is protective against oxidative stress, in VCIND versus cognitively normal CAD controls (CN). GPX activity was also evaluated as a biomarker of cognition, particularly verbal memory. Methods: 120 CAD patients with VCIND (1SD below norms on executive function or verbal memory (VM)) or without (CN) participated in exercise rehabilitation for 24 weeks. Neurocognitive and cardiopulmonary fitness (VO2 peak) assessments and plasma were collected at baseline and 24-weeks. Results: GPX was higher in VCIND compared to CN (F1,119 = 3.996, p = 0.048). Higher GPX was associated with poorer baseline VM (β= –0.182, p = 0.048), and longitudinally with VM decline controlling for sex, body mass index, VO2 peak, and education (b[SE] = –0.02[0.01], p = 0.004). Only CN participants showed improved VM performance with increased fitness (b[SE] = 1.30[0.15], p <  0.005). Conclusion: GPX was elevated in VCIND consistent with a compensatory response to persistent oxidative stress. Increased GPX predicted poorer cognitive outcomes (verbal memory) in VCIND patients despite improved fitness.

scholarly journals Selenium levels and glutathione peroxidase activity in patients with ataxia-telangiectasia: association with oxidative stress and lipid status biomarkers

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Itana Gomes Alves Andrade ◽  
Fabíola Isabel Suano-Souza ◽  
Fernando Luiz Affonso Fonseca ◽  
Carolina Sanchez Aranda Lago ◽  
Roseli Oselka Saccardo Sarni
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Oxidized Ldl ◽  
Reference Value ◽  
Glutathione Peroxidase Activity ◽  
Oxidative Stress Biomarkers ◽  
Apo B ◽  
Lipid Status ◽  
And Oxidative Stress

Abstract Introduction Ataxia-Telangiectasia (A-T) is a multi-system disorder that may be associated with endocrine changes, oxidative stress in addition to inflammation. Studies suggest that selenium is a trace element related to protection against damage caused by oxidative stress. Objective To describe the plasma levels of selenium and erythrocyte glutathione peroxidase activity in A-T patients and to relate them to oxidative stress and lipid status biomarkers. Methods This is a cross-sectional and controlled study evaluating 22 A-T patients (age median, 12.2 years old) matched by gender and age with 18 healthy controls. We evaluated: nutritional status, food intake, plasma selenium levels, erythrocyte glutathione peroxidase activity, lipid status, inflammation and oxidative stress biomarkers. Results Adequate levels of selenium were observed in 24/36 (66.7%) in this evaluated population. There was no statistically significant difference between the groups in selenium levels [47.6 μg/L (43.2–57.0) vs 54.6 (45.2–62.6) μg/dL, p = 0.242]. Nine of A-T patients (41%) had selenium levels below the reference value. The A-T group presented higher levels of LDL-c, non-HDL-c, oxidized LDL, Apo B, Apo-B/Apo-A-I1, LDL-c/HDL-c ratio, malondialdehyde [3.8 µg/L vs 2.8 µg/L, p = 0.029] and lower Apo-A-I1/HDL-c and glutathione peroxidase activity [7300 U/L vs 8686 U/L, p = 0.005]. Selenium levels were influenced, in both groups, independently, by the concentrations of oxidized LDL, malonaldehyde and non-HDL-c. The oxidized LDL (AUC = 0.849) and ALT (AUC = 0.854) were the variables that showed the greatest discriminatory power between groups. Conclusion In conclusion, we observed the presence of selenium below the reference value in nearly 40% and low GPx activity in A-T patients. There was a significant, inverse and independent association between selenium concentrations and oxidative stress biomarkers. Those data reinforce the importance of assessing the nutritional status of selenium in those patients.

scholarly journals OXIDATIVE STRESS MARKERS IN THE LUNGS UNDER EXPERIMENTAL HYPEROXIA WITH INHALED LIPOSOMES BASED ON EGG LECITHIN

Biomeditsina ◽  
2019 ◽  
pp. 49-58
Author(s):  
I. L. Kotovich ◽  
Zh. A. Rutkovskaya ◽  
A. D. Taganovich
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Alpha Tocopherol ◽  
Oxygen Species ◽  
Glutathione Peroxidase Activity ◽  
Diene Conjugates ◽  
Hyperoxia Exposure ◽  
Egg Lecithin

Oxidative stress is considered to be a factor leading to lung damage in premature infants. The aim of this study was to investigate the effect of inhaled antioxidants incorporated into egg lecithin liposomes on the indicators of oxidative stress in the lungs of newborn guinea pigs under experimental hyperoxia (3 days). Bronchoalveolar lavage fl uid (BALF) was used as a material for the study. Under hyperoxia exposure, inhalation of liposomes containing N-acetylcysteine and alpha-tocopherol contributed to the suppression of the reactive oxygen species production by cells, normalization of glutathione peroxidase activity and carbonyls content, while not affecting the level of diene conjugates in BALF. The introduction of retinoid-containing liposomes (retinol and retinoic acid) under hyperoxia was accompanied by normalization of glutathione peroxidase activity as well as the content of protein oxidation products in BALF, while the generation of reactive oxygen species remained enhanced, and the diene conjugates and thiobarbituric acid reactive products exceeded the levels in animals exposed to hyperoxia alone. Thus, the inhaled liposomes containing retinoids and egg lecithin exhibit not only anti-, but also a prooxidant effect in the lungs under hyperoxia exposure, unlike the liposomal forms of N-acetylcysteine and alpha-tocopherol.

scholarly journals Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI

2019 ◽  
Vol 42 (1) ◽  
pp. 1-8 ◽  
Author(s):  
José Araújo de Oliveira-Silva ◽  
Joyce Umbelino Pinto Yamamoto ◽  
Renata Bernardes de Oliveira ◽  
Vaneisse Cristina Lima Monteiro ◽  
Beatriz Jurkiewcz Frangipani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Selenium Supplementation ◽  
Glutathione Peroxidase Activity ◽  
Stress Assessment ◽  
Mucopolysaccharidosis I

Stimulation of Glutathione Peroxidase Activity Decreases HIV Type 1 Activation after Oxidative Stress

1994 ◽  
Vol 10 (11) ◽  
pp. 1451-1461 ◽  
Author(s):  
CHRISTINE SAPPEY ◽  
SYLVIE LEGRAND-POELS ◽  
MARTIN BEST-BELPOMME ◽  
ALAIN FAVIER ◽  
BERNARD RENTIER ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Glutathione Peroxidase Activity ◽  
Hiv Type 1 ◽  
Stimulation Of

Vitamin E protection of obesity-enhanced vascular calcification in uremic rats

2014 ◽  
Vol 306 (4) ◽  
pp. F422-F429 ◽  
Author(s):  
A. Peralta-Ramírez ◽  
A. Montes de Oca ◽  
A. I. Raya ◽  
C. Pineda ◽  
I. López ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Calcium Concentration ◽  
Soft Tissues ◽  
Zucker Rats ◽  
Glutathione Peroxidase Activity ◽  
Obese Rats

This study aimed to determine the extent of extraskeletal calcification in uremic Zucker rats, by comparing obese and lean phenotypes, and to evaluate the influence of vitamin E (VitE) on the development of calcifications in both uremic rats and human vascular smooth muscle cells (HVSMCs) cultured in vitro. Zucker rats of lean and obese phenotypes with normal renal function [control (C); C-lean and C-obese groups] and with uremia [5/6 nephrectomy (Nx); Nx-lean and Nx-obese groups] and uremic rats treated with VitE (Nx-lean + VitE and Nx-obese + VitE groups) were studied. Uremic groups were subjected to Nx, fed a 0.9% phosphorus diet, and treated with calcitriol (80 ng/kg ip). The aortic calcium concentration was significantly higher ( P < 0.05) in Nx-obese rats (10.0 ± 2.1 mg/g tissue) than in Nx-lean rats (3.6 ± 1.3 mg/g tissue). A decrease in plasma glutathione peroxidase activity was observed in Nx-obese rats compared with Nx-lean rats (217.2 ± 18.2 vs. 382.3 ± 15.5 nmol·min−1·ml−1, P < 0.05). Treatment with VitE restored glutathione peroxidase activity and reduced the aortic calcium concentration to 4.6 ± 1.3 mg/g tissue. The differences in mineral deposition between Nx-lean, Nx-obese, Nx-lean + VitE, and Nx-obese + VitE rats were also evidenced in other soft tissues. In HVSMCs incubated with high phosphate, VitE also prevented oxidative stress and reduced calcium content, bone alkaline phosphatase, and gene expression of core-binding factor-α1. In conclusion, uremic obese rats develop more severe calcifications than uremic lean rats and VitE reduces oxidative stress and vascular calcifications in both rats and cultures of HVSMCs.

Resistance of Cultured Human Skin Fibroblasts from Old and Young Donors to Oxidative Stress and Their Glutathione Peroxidase Activity

Gerontology ◽  
2004 ◽  
Vol 50 (4) ◽  
pp. 193-199 ◽  
Author(s):  
Mitsuyoshi Matsuo ◽  
Hidefumi Ikeda ◽  
Takehiko Sugihara ◽  
Satomi Horiike ◽  
Yuri Okano ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Human Skin ◽  
Peroxidase Activity ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Glutathione Peroxidase Activity

scholarly journals Changes in Oxidative Stress, Inflammation, and Muscle Damage Markers Following Diet and Beetroot Juice Supplementation in Elite Fencers

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 571
Author(s):  
Lucyna Kozłowska ◽  
Olga Mizera ◽  
Jolanta Gromadzińska ◽  
Beata Janasik ◽  
Karolina Mikołajewska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Serum Concentration ◽  
Muscle Damage ◽  
Peroxidase Activity ◽  
Activity Level ◽  
Beetroot Juice ◽  
Glutathione Peroxidase Activity ◽  
Ldh Activity ◽  
Β Carotene

The aim of this study was to assess the impact of diet and active substances in beetroot juice on the parameters of oxidative stress, inflammation, and muscle damage as well as on the maximum rate of oxygen uptake (VO2max) in elite fencers (10 women, 10 men). Athletes during four weeks realized dietary recommendations (ID) and, after that, diet with freeze-dried beetroot juice supplementation (ID&BEET). At baseline and after each stage, fasting antioxidants, biomarkers of oxidative stress, inflammation, and skeletal muscle damage were measured, and a VO2max test was performed. Only after ID&BEET was a significant increase of VO2max observed, and changes of this parameter were negatively related with changes of serum lactate dehydrogenase (∆LDH) activity, as well as with serum ∆β-carotene and malondialdehyde concentration (∆MDA). Additionally, positive relationships were observed between ∆β-carotene versus changes of the serum concentration of advanced oxidation protein products (∆AOPP), changes of serum glutathione peroxidase activity (∆GPx3) versus both changes of physical activity level and ∆LDH, as well as erythrocyte glutathione peroxidase activity (∆GPx1) versus ∆LDH. To summarize, we showed that long-term beetroot juice supplementation increases lipid peroxidation, and improvement of VO2max after ID&BEET seems to be dependent on LDH activity, as well as on the serum concentration of MDA and β-carotene.

Salicylic acid modulates oxidative stress and glutathione peroxidase activity in the rat colon

2005 ◽  
Vol 70 (6) ◽  
pp. 888-893 ◽  
Author(s):  
Janice E. Drew ◽  
John R. Arthur ◽  
Andrew J. Farquharson ◽  
Wendy R. Russell ◽  
Philip C. Morrice ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Salicylic Acid ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Rat Colon ◽  
Glutathione Peroxidase Activity

ACE inhibitor reduces growth factor receptor expression and signaling but also albuminuria through B2-kinin glomerular receptor activation in diabetic rats

2007 ◽  
Vol 293 (4) ◽  
pp. F1083-F1092 ◽  
Author(s):  
Julien Allard ◽  
Marie Buléon ◽  
Eric Cellier ◽  
Isabelle Renaud ◽  
Christiane Pecher ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Growth Factor ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Early Phase ◽  
Receptor Activation ◽  
Glutathione Peroxidase Activity ◽  
Kinin Receptor ◽  
Hoe 140 ◽  
Growth Factor Pathways

Diabetic nephropathy (DN) is associated with increased oxidative stress, overexpression and activation of growth factor receptors, including those for transforming growth factor-β1 (TGF-β-RII), platelet-derived growth factor (PDGF-R), and insulin-like growth factor (IGF1-R). These pathways are believed to represent pathophysiological determinants of DN. Beyond perfect glycemic control, angiotensin-converting enzyme inhibitors (ACEI) are the most efficient treatment to delay glomerulosclerosis. Since their mechanisms of action remain uncertain, we investigated the effect of ACEI on the glomerular expression of these growth factor pathways in a model of streptozotocin-induced diabetes in rats. The early phase of diabetes was found to be associated with an increase in glomerular expression of IGF1-R, PDGF-R, and TGF-β-RII and activation of IRS1, Erk 1/2, and Smad 2/3. These changes were significantly reduced by ACEI treatment. Furthermore, ACEI stimulated glutathione peroxidase activity, suggesting a protective role against oxidative stress. ACEI decreased ANG II production but also increased bradykinin bioavailability by reducing its degradation. Thus the involvement of the bradykinin pathway was investigated using coadministration of HOE-140, a highly specific nonpeptidic B2-kinin receptor antagonist. Almost all the previously described effects of ACEI were abolished by HOE-140, as was the increase in glutathione peroxidase activity. Moreover, the well-established ability of ACEI to reduce albuminuria was also prevented by HOE-140. Taken together, these data demonstrate that, in the early phase of diabetes, ACEI reverse glomerular overexpression and activation of some critical growth factor pathways and increase protection against oxidative stress and that these effects involve B2-kinin receptor activation.

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