Genetic polymorphisms of IL-6-174 and IL-10-1082 in full term neonates with late onset blood stream infections

Neonatal pneumonia is a serious respiratory infectious disease with a high rate of case fatality in developing countries. Salivary cytokines could serve as interesting noninvasive markers in the diagnosis of neonatal pneumonia. The aim was to assess the diagnostic role of salivary and serum interleukin-6 (IL-6), C-reactive protein/mean platelet volume (CRP/MPV) ratio, and the combination of these markers in the diagnosis of late-onset neonatal pneumonia in full-term neonates. Seventy full-term neonates, 35 with late-onset neonatal pneumonia and 35 controls, were enrolled in this prospective case-control study. Complete blood count (CBC), salivary and serum IL-6, and CRP concentrations were measured for all the study subjects. The sensitivity, specificity, positive predictive value, and negative predictive value of salivary IL-6, serum IL-6, and CRP/MPV ratio for the diagnosis of late-onset neonatal pneumonia were determined. At the cutoff point of >34 pg/ml, salivary IL-6 showed 82.86% sensitivity and 91.43% specificity. CRP/MPV ratio showed a sensitivity of 97.14% and specificity of 85.71% at a cutoff value > 0.88 . The combination of salivary IL-6 and CRP/MPV ratio improved the sensitivity and specificity to 100%. The current study shows for the first time that both salivary IL-6 and CRP/MPV ratio are suitable markers for the diagnosis of late-onset neonatal pneumonia in full-term neonates.

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Assessment of thyroid hormones in full-term neonates with late-onset sepsis

Indian Journal of Child Health ◽

10.32677/ijch.2021.v08.i06.006 ◽

2021 ◽

Vol 8 (6) ◽

pp. 225-230

Author(s):

Dhananjay Singh ◽

Amit Agrawal ◽

Jyotsna Shrivastava

Keyword(s):

Thyroid Hormones ◽

Late Onset ◽

Full Term ◽

Term Neonates ◽

Late Onset Sepsis

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Association between DNA Damage and Serum Levels of Copper, Zinc, and Selenium in Full-Term Neonates with Late-Onset Sepsis

Journal of Pediatric Infectious Diseases ◽

10.1055/s-0040-1717126 ◽

2020 ◽

Vol 15 (06) ◽

pp. 299-306

Author(s):

Aliaa Ahmed Wahby ◽

Maha Elwassif ◽

Mai Magdy ◽

Tamer A. Abdel Hamid ◽

Alshaymaa A. Ibrahim

Keyword(s):

Risk Factors ◽

Dna Damage ◽

Trace Elements ◽

Neonatal Sepsis ◽

Late Onset ◽

Serum Levels ◽

Full Term ◽

Assay Method ◽

Term Neonates ◽

Late Onset Sepsis

Abstract Objective The alteration in certain trace elements is usually associated with impaired immune function and higher oxidative stress. Therefore, these elements are suggested to play an important role in the pathogenesis of neonatal sepsis. We aimed to evaluate copper (Cu), zinc (Zn), and selenium (Se) serum levels in full-term neonates with late-onset sepsis (LOS) and correlate these levels with DNA damage and other risk factors of sepsis. Methods The study included a group of 100 neonates diagnosed with sepsis serving as the case group and another one of 60 neonates serving as the control group. DNA damage was assessed using the comet assay method and trace elements were measured using inductively coupled plasma mass spectrometry. Results Compared with controls, the percentage of DNA damage was significantly elevated in patients with sepsis, while serum levels of Cu, Zn, and Se were markedly decreased (p = 0.001). A strong negative correlation was revealed between Se and DNA damage (r = −0.6, p = 0.001). However, no correlations were found between Cu or Zn and DNA damage. Univariate logistic regression analysis revealed that DNA damage as well as Cu, Zn, and Se serum levels can be considered as relevant risk factors for neonatal sepsis (p = 0.008, 0.004, 0.004, and 0.003, respectively). Receiver-operating characteristic curve analysis showed that the strongest indicator for neonatal sepsis was Se (area under the curve [AUC] = 0.94, confidence interval [CI] = 0.9–0.98, p = 0.001), followed by Cu (AUC = 0.9, CI = 0.85–0.96, p = 0.001), and then Zn (AUC = 0.87, CI = 0.8–0.93, p = 0.001). Conclusion The percentage of DNA damage may help in the assessment of neonatal sepsis severity. Altered levels of Cu, Zn, and Se may play significant role in the pathogenesis of neonatal sepsis. Se serum level is strongly correlated with percentage of DNA damage. Therefore, Se can predict the severity of LOS.

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Red Cell Distribution Width and Neutrophil to Lymphocyte Ratio as Early Indicators of Late-Onset Sepsis in Full-Term Neonates : Relation to Mortality

Egyptian Journal of Pediatrics ◽

10.12816/0054715 ◽

2019 ◽

Vol 36 (3-4) ◽

pp. 457-468

Author(s):

Noha R. Mohamed ◽

Dina M. Shinkar

Keyword(s):

Late Onset ◽

Full Term ◽

Neutrophil To Lymphocyte Ratio ◽

Red Cell Distribution Width ◽

Red Cell ◽

Cell Distribution ◽

Distribution Width ◽

Term Neonates ◽

Lymphocyte Ratio ◽

Late Onset Sepsis

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Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates

Journal of Immunology Research ◽

10.1155/2021/4884537 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Ahmed Omran ◽

Hazem Sobh ◽

Mohamed Osama Abdalla ◽

Sonya El-Sharkawy ◽

Ahmed R. Rezk ◽

...

Keyword(s):

Neonatal Sepsis ◽

Mean Platelet Volume ◽

Late Onset ◽

Interleukin 10 ◽

Full Term ◽

C Reactive Protein ◽

Platelet Volume ◽

Term Neonates ◽

Reactive Protein ◽

Late Onset Sepsis

Salivary markers could serve as potential noninvasive markers in the diagnosis of neonatal infections. We aimed to investigate the diagnostic role of salivary and serum interleukin 10 (IL-10), C-reactive protein (CRP), mean platelet volume (MPV), and CRP/MPV ratio in the diagnosis of late-onset neonatal sepsis in full-term neonates. Seventy full-term neonates were enrolled in this prospective case-control study, 35 with late-onset neonatal sepsis, and 35 controls. Salivary IL-10, serum IL-10, and CRP concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Complete blood (CBC) count was measured by an automated blood cell counter. The salivary IL-10, serum IL-10, CRP, MPV, and CRP/MPV ratio levels were much higher in neonates with late-onset sepsis than in control ( 220 ± 150 vs. 18 ± 9 pg / ml , P < 0.001 ), ( 316 ± 198 vs. 23.7 ± 14 pg / ml , P < 0.001 ), ( 78.2 ± 34 vs. 3.3 ± 1.7 mg / L , P < 0.001 ), ( 11.2 ± 0.9 vs. 8.6 ± 0.4 fL ), and ( 7.08 ± 3.3 vs. 0.4 ± 0.2 , P < 0.001 ), respectively. At the cutoff point of >31 pg/ml, salivary IL-10 showed 97.1% sensitivity and 94.3% specificity. Serum IL-10 at a cutoff value of ≥33.6 pg/ml had a sensitivity of 97.1% and specificity of 80%. MPV showed a sensitivity of 100% and specificity of 94.4% at a cutoff value ≥ 9.2 fL . CRP/MPV ratio showed a sensitivity of 100% and specificity of 97.1% at a cutoff value > 0.9 . Salivary and serum IL-10 showed a positive correlation with CRP and CRP/MPV ratio in septic neonates. The current study shows for the first time that both salivary IL-10 and CRP/MPV showed statistically significant differences between neonates with late-onset sepsis and controls. Accordingly, salivary IL-10 could serve as a potential noninvasive biomarker for the diagnosis of late-onset sepsis in full-term neonates.

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