scholarly journals Assessment of thyroid hormones in full-term neonates with late-onset sepsis

2021 ◽  
Vol 8 (6) ◽  
pp. 225-230
Author(s):  
Dhananjay Singh ◽  
Amit Agrawal ◽  
Jyotsna Shrivastava
Keyword(s):  
Thyroid Hormones ◽  
Late Onset ◽  
Full Term ◽  
Term Neonates ◽  
Late Onset Sepsis

Association between DNA Damage and Serum Levels of Copper, Zinc, and Selenium in Full-Term Neonates with Late-Onset Sepsis

2020 ◽  
Vol 15 (06) ◽  
pp. 299-306
Author(s):  
Aliaa Ahmed Wahby ◽  
Maha Elwassif ◽  
Mai Magdy ◽  
Tamer A. Abdel Hamid ◽  
Alshaymaa A. Ibrahim
Keyword(s):  
Risk Factors ◽  
Dna Damage ◽  
Trace Elements ◽  
Neonatal Sepsis ◽  
Late Onset ◽  
Serum Levels ◽  
Full Term ◽  
Assay Method ◽  
Term Neonates ◽  
Late Onset Sepsis

Abstract Objective The alteration in certain trace elements is usually associated with impaired immune function and higher oxidative stress. Therefore, these elements are suggested to play an important role in the pathogenesis of neonatal sepsis. We aimed to evaluate copper (Cu), zinc (Zn), and selenium (Se) serum levels in full-term neonates with late-onset sepsis (LOS) and correlate these levels with DNA damage and other risk factors of sepsis. Methods The study included a group of 100 neonates diagnosed with sepsis serving as the case group and another one of 60 neonates serving as the control group. DNA damage was assessed using the comet assay method and trace elements were measured using inductively coupled plasma mass spectrometry. Results Compared with controls, the percentage of DNA damage was significantly elevated in patients with sepsis, while serum levels of Cu, Zn, and Se were markedly decreased (p = 0.001). A strong negative correlation was revealed between Se and DNA damage (r = −0.6, p = 0.001). However, no correlations were found between Cu or Zn and DNA damage. Univariate logistic regression analysis revealed that DNA damage as well as Cu, Zn, and Se serum levels can be considered as relevant risk factors for neonatal sepsis (p = 0.008, 0.004, 0.004, and 0.003, respectively). Receiver-operating characteristic curve analysis showed that the strongest indicator for neonatal sepsis was Se (area under the curve [AUC] = 0.94, confidence interval [CI] = 0.9–0.98, p = 0.001), followed by Cu (AUC = 0.9, CI = 0.85–0.96, p = 0.001), and then Zn (AUC = 0.87, CI = 0.8–0.93, p = 0.001). Conclusion The percentage of DNA damage may help in the assessment of neonatal sepsis severity. Altered levels of Cu, Zn, and Se may play significant role in the pathogenesis of neonatal sepsis. Se serum level is strongly correlated with percentage of DNA damage. Therefore, Se can predict the severity of LOS.

scholarly journals Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Ahmed Omran ◽  
Hazem Sobh ◽  
Mohamed Osama Abdalla ◽  
Sonya El-Sharkawy ◽  
Ahmed R. Rezk ◽  
...  
Keyword(s):  
Neonatal Sepsis ◽  
Mean Platelet Volume ◽  
Late Onset ◽  
Interleukin 10 ◽  
Full Term ◽  
C Reactive Protein ◽  
Platelet Volume ◽  
Term Neonates ◽  
Reactive Protein ◽  
Late Onset Sepsis

Salivary markers could serve as potential noninvasive markers in the diagnosis of neonatal infections. We aimed to investigate the diagnostic role of salivary and serum interleukin 10 (IL-10), C-reactive protein (CRP), mean platelet volume (MPV), and CRP/MPV ratio in the diagnosis of late-onset neonatal sepsis in full-term neonates. Seventy full-term neonates were enrolled in this prospective case-control study, 35 with late-onset neonatal sepsis, and 35 controls. Salivary IL-10, serum IL-10, and CRP concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Complete blood (CBC) count was measured by an automated blood cell counter. The salivary IL-10, serum IL-10, CRP, MPV, and CRP/MPV ratio levels were much higher in neonates with late-onset sepsis than in control ( 220 ± 150 vs. 18 ± 9   pg / ml , P < 0.001 ), ( 316 ± 198 vs. 23.7 ± 14   pg / ml , P < 0.001 ), ( 78.2 ± 34 vs. 3.3 ± 1.7   mg / L , P < 0.001 ), ( 11.2 ± 0.9 vs. 8.6 ± 0.4   fL ), and ( 7.08 ± 3.3 vs. 0.4 ± 0.2 , P < 0.001 ), respectively. At the cutoff point of >31 pg/ml, salivary IL-10 showed 97.1% sensitivity and 94.3% specificity. Serum IL-10 at a cutoff value of ≥33.6 pg/ml had a sensitivity of 97.1% and specificity of 80%. MPV showed a sensitivity of 100% and specificity of 94.4% at a cutoff   value ≥ 9.2   fL . CRP/MPV ratio showed a sensitivity of 100% and specificity of 97.1% at a cutoff   value > 0.9 . Salivary and serum IL-10 showed a positive correlation with CRP and CRP/MPV ratio in septic neonates. The current study shows for the first time that both salivary IL-10 and CRP/MPV showed statistically significant differences between neonates with late-onset sepsis and controls. Accordingly, salivary IL-10 could serve as a potential noninvasive biomarker for the diagnosis of late-onset sepsis in full-term neonates.

Diagnostic value of assessment of Serum Cortisol, Hepcidin and Thyroid Hormone Levels in Neonates with Late Onset Sepsis

2020 ◽  
Vol 20 ◽  
Author(s):  
Adel Hagag ◽  
Mohamed S Elfarargy ◽  
Reham Lyonis ◽  
Ghada M Al-Ashmawy
Keyword(s):  
Thyroid Hormones ◽  
Antibiotic Therapy ◽  
Neonatal Sepsis ◽  
Late Onset ◽  
Serum Cortisol ◽  
Control Group ◽  
Serum Free ◽  
Group I ◽  
Late Onset Sepsis ◽  
Serum Hepcidin

Background: Neonatal sepsis is a clinical syndrome characterized by symptoms and signs of infection in the first twenty eight days of life. Serum thyroid, cortisol and hepcidin are affected by neonatal sepsis. Aim of the work: The aim of this study was to assess the predictive value of serum thyroid hormones including free triiodothyronine (free TT3) and free tetraiodothyronine (free TT4), serum cortisol and hepcidin levels through comparison of their concentrations between normal neonates and neonates with high probable late onset sepsis. Patients and Methods: This case control study was carried out on 40 neonates with suspected high probable late onset neonatal sepsis based on clinical and laboratory finding who were admitted to NICU of Pediatric Department, Tanta University, Egypt in the period from April 2017 to May 2019 (group I) and 40 healthy neonates matched in age and sex as a control group (group II). For patients and controls; blood culture, highly sensitive C‑reactive protein (H-s CRP), serum hepcidin, serum cortisol and thyroid hormones levels including free TT3 and free TT4 were assessed. Results: There were no significant differences between studied groups as regard weight, gestational age, sex and mode of delivery. H-s CRP, serum cortisol and hepcidin were significantly higher in group I than group II while serum free TT3 and free TT4 were significantly lower in group I compared with controls. There was significantly lower H-s CRP, serum hepcidin and cortisol and significantly higher serum free TT3 and free TT4 in group I after antibiotic therapy compared to the same group before treatment while there were no significant differences between group I after antibiotic therapy and control group as regard the same parameters. There were significant positive correlation between H-s CRP and serum hepcidin and cortisol in group I while there was significant negative correlation between H-s CRP and free TT3 and free TT4. ROC curve of specificity and sensitivity of H-s CRP, serum hepcidin, cortisol, free TT3 and free TT4 in prediction of neonatal sepsis shows that serum hepcidin had the highest sensitivity and specificity with 95% and 90% respectively followed by serum cortisol, H-s CRP, free TT3 and lastly free TT4. Conclusion and recommendations: Neonates with high probable sepsis had significantly higher serum cortisol and hepcidin and significantly lower free TT3 and free TT4 compared with healthy neonates. These findings may arouse our attention about the use of these markers in diagnosis of in neonatal sepsis which can lead to early treatment and subsequently better prognosis.

Genetic polymorphisms of IL-6-174 and IL-10-1082 in full term neonates with late onset blood stream infections

2015 ◽  
Vol 04 (04) ◽  
pp. 357-365 ◽  
Author(s):  
Mohamed El-Naggar ◽  
Ghada El-Nady ◽  
Rawia Badr ◽  
Medhat El-Daker ◽  
Hesham Abdel-Hady
Keyword(s):  
Genetic Polymorphisms ◽  
Late Onset ◽  
Blood Stream ◽  
Full Term ◽  
Blood Stream Infections ◽  
Term Neonates

Lactoferrin and prevention of late-onset sepsis in the pre-term neonates

2010 ◽  
Vol 86 (1) ◽  
pp. 59-61 ◽  
Author(s):  
P. Manzoni ◽  
L. Decembrino ◽  
I. Stolfi ◽  
L. Pugni ◽  
M. Rinaldi ◽  
...  
Keyword(s):  
Late Onset ◽  
Term Neonates ◽  
Late Onset Sepsis

Serum Cortisol, Hepcidin and Thyroid Hormone Levels in Neonates with Late Onset ‎Sepsis; Methodological Issues on Diagnostic and Prognostic Studies

2020 ◽  
Vol 20 ◽  
Author(s):  
Siamak Sabour
Keyword(s):  
Thyroid Hormones ◽  
Likelihood Ratio ◽  
Roc Curve ◽  
Neonatal Sepsis ◽  
Predictive Value ◽  
Late Onset ◽  
Serum Cortisol ◽  
Methodological Issues ◽  
Late Onset Sepsis ◽  
Serum Hepcidin

: I was interested in reading the paper by Hagag A and colleagues that was published in the March 2020 edition of the Infect Disord Drug Targets [1]. Neonatal sepsis is a clinical syndrome characterized by symptoms and signs of infection in the first twenty-eight days of life. Serum thyroid, cortisol and hepcidin are affected by neonatal sepsis. The purpose of the authors was to assess the predictive value of serum thyroid hormones including free triiodothyronine (free TT3) and free tetraiodothyronine (free TT4), serum cortisol and hepcidin levels through comparison of their concentrations between normal neonates and neonates with high probable late onset sepsis. They carried out a case control study on 40 neonates with suspected high probable late onset neonatal sepsis and 40 healthy neonates matched in age and sex as a control group (group II). For patients and controls; blood culture, highly sensitive C-reactive protein (H-s CRP), serum hepcidin, serum cortisol and thyroid hormones levels including free TT3 and free TT4 were assessed. They reported a significant positive correlation between H-s CRP and serum hepcidin and cortisol in group I while there was significant negative correlation between H-s CRP and free TT3 and free TT4. ROC curve of specificity and sensitivity of H-s CRP, serum hepcidin, cortisol, free TT3 and free TT4 in prediction of neonatal sepsis shows that serum hepcidin had the highest sensitivity and specificity with 95% and 90% respectively followed by serum cortisol, H-s CRP, free TT3 and lastly free TT4. First, reproducibility and validity (accuracy) are two different methodological issues of diagnostic studies [2]. Second, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) as well as likelihood ratios [likelihood ratio positive (ranging from 1 to infinity; the higher the LR+, the more accurate the test) and likelihood ratio negative (ranging from 0 to 1; the lower the LR-, the more accurate the test)] are among the estimates to assess validity (accuracy) of a diagnostic test [2-6]. Third, receiver operative characteristic (ROC) curve is usually used to assess diagnostic accuracy (discrimination) of a diagnostic model and has nothing to do with prediction of neonatal sepsis. Finally, logistic regression analyses have nothing to do with prediction studies. For prediction, we need at least two different cohort datasets or at least one cohort dataset, splitting them for development and validation of our model [2,7,8]. They concluded that neonates with high probable sepsis had significantly higher serum cortisol and hepcidin and significantly lower free TT3 and free TT4 compared with healthy neonates. They claimed that higher serum cortisol and hepcidin and lower free TT3 and free TT4 can lead to early diagnosis and subsequently better prognosis. Such conclusion should be supported by the above-mentioned methodological issues. Otherwise, misdiagnosis and mismanagement of the patient may occur.

scholarly journals miRNA-23b as a biomarker of culture-positive neonatal sepsis

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Ahlam Fatmi ◽  
Sid Ahmed Rebiahi ◽  
Nafissa Chabni ◽  
Hanane Zerrouki ◽  
Hafsa Azzaoui ◽  
...  
Keyword(s):  
Blood Culture ◽  
Correlation Coefficient ◽  
Neonatal Sepsis ◽  
Late Onset ◽  
Protective Role ◽  
Full Term ◽  
Late Onset Sepsis ◽  
Early Onset Sepsis ◽  
Early Sepsis ◽  
Negative Controls

Abstract Background Neonatal sepsis remains an important cause of morbidity and mortality. The ability to quickly and accurately diagnose neonatal sepsis based on clinical assessments and laboratory blood tests remains difficult, where haemoculture is the gold standard for detecting bacterial sepsis in blood culture. It is also very difficult to study because neonatal samples are lacking. Methods Forty-eight newborns suspected of sepsis admitted to the Neonatology Department of the Mother-Child Specialized Hospital of Tlemcen. From each newborn, a minimum of 1–2 ml of blood was drawn by standard sterile procedures for blood culture. The miRNA-23b level in haemoculture was evaluated by RT-qPCR. Results miR-23b levels increased in premature and full-term newborns in early onset sepsis (p < 0.001 and p < 0.005 respectively), but lowered in late onset sepsis in full-term neonates (p < 0.05) compared to the respective negative controls. miR-23b levels also increased in late sepsis in the negative versus early sepsis negative controls (p < 0.05). miR-23b levels significantly lowered in the newborns who died from both sepsis types (p < 0.0001 and p < 0.05 respectively). In early sepsis, miR-23b and death strongly and negatively correlated (correlation coefficient = − 0.96, p = 0.0019). In late sepsis, miRNA-23b and number of survivors (correlation coefficient = 0.70, p = 0.506) positively correlated. Conclusions Lowering miR-23b levels is an important factor that favours sepsis development, which would confirm their vital protective role, and strongly suggest that they act as a good marker in molecular diagnosis and patient monitoring.

scholarly journals Expression of Serum Cytokines Profile in Neonatal Sepsis

2020 ◽  
Author(s):  
Mengjiao Kuang ◽  
Suipeng Chen ◽  
Shirui Huang ◽  
Binbin Gong ◽  
Suzhen Lin ◽  
...  
Keyword(s):  
Neonatal Sepsis ◽  
Late Onset ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Cytokine ◽  
Term Neonates ◽  
Late Onset Sepsis ◽  
Early Onset Sepsis ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Abstract BackgroundSepsis remains a major cause of neonatal death, but its underlying pathological mechanisms are poorly understood. Methods To characterize the serum cytokine/chemokine profile in neonates with sepsis, we enrolled 40 full-term neonates with sepsis and 19 neonates without infection as controls. Forty cytokines/chemokines in serum were analyzed using the Luminex Bead Immunoassay System. Serum IL-17 was measured using an enzyme-linked immunosorbent assay. ResultsOur results showed that serum IL-6, IL-8, TNF-α, IL-1β, MIF, CXCL13, CXCL1, CXCL2, CXCL5, CXCL6, CXCL16, CCL27, CCL2, CCL8, CCL3, CCL20, CCL23, CCL27, and CX3CL1 levels were significantly increased in neonates with sepsis compared to those in the control group (all p<0.05). The levels of serum CXCL6, CXCL1, IL-6, CXCL10, CXCL11, CCL20, and IL-17 were higher in late-onset sepsis (LOS) than in early-onset sepsis (EOS) (all p<0.05). Conversely, serum IL-16, CXCL16, and CCL22 were lower in LOS than in EOS (all p<0.05). The levels of CX3CL1, CXCL2, CCL8, and TNF-α were all positively correlated with SOFA scores. ConclusionOur findings revealed that excessive pro-inflammatory cytokines might be involved in neonatal sepsis. In addition, chemokines significantly increased the recruitment of immune cells after infection to participate in the anti-infection defense of neonates, but this could lead to damage.

scholarly journals Hafnia alvei causing late onset sepsis in neonates: report of two cases and review of literature

2018 ◽  
Vol 5 (2) ◽  
pp. 673
Author(s):  
Laxman Basani ◽  
Roja Aepala
Keyword(s):  
Antibiotic Therapy ◽  
Pediatric Patients ◽  
Neonatal Period ◽  
Late Onset ◽  
Review Of Literature ◽  
Term Neonates ◽  
Gram Negative ◽  
Hafnia Alvei ◽  
Late Onset Sepsis ◽  
Compromised Patients

Hafnia alvei, a Gram negative motile bacillus that belongs to Enterobacteriaceae family is rarely associated with infection in pediatric patients and is exceptionally rare in the neonatal period. H. alvei is ubiquitous in the environment, causing infections in debilitated and immuno-compromised patients with few cases being reported in neonates. We report two cases of late onset sepsis in term neonates caused by H. alvei that were successfully treated in our unit. To the best of our knowledge, infection due to H. alvei has not been reported in neonates from India. Hafnia alvei causes infection rarely in neonates. Because it can cause nosocomial outbreaks, awareness regarding this uncommon pathogen and initiation of appropriate antibiotic therapy improves the outcome and prevents mortality.

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