Effect of organic additives on storage stability of camel liver catalase against environmental conditions

2021 ◽  
pp. 1-7
Author(s):  
Mustafa Zeyadi

The storage stability of catalase is very low under practical reaction conditions. Therefore, this study is aimed to evaluate the storage stability of catalase from camel liver by treated it with polyethylene glycol (PEG), glycerol, bovine serum albumin (BSA) and glucose against different storage conditions. The effect of some additives as stabilizers on the storage stability of camel liver catalase at 4°C and 30°C after 30 and 90 days was studied. The enzyme with additives at 5%concentration was more stable at 4 and 30°C for 90 days of incubation than native enzyme. The activity of catalase was more thermal stable in presence of 5%additive. The activity of enzyme with 5%BSA and glucose was retained 60%of its activity at 60°C. At pH 11 the catalase with glucose retained 30%of its activity. The native enzyme lost 80%of its activity in presence of 5 mM β-mercaptoethanol, where the enzyme with additive retained 30–46%of its activity. At 8 M urea, the native enzyme and enzyme with 5%of all additives retained 28%and 61–77%of its activity, respectively. The catalase with additives retained 50–90%of its activity in presence of different dyes. The results appeared that the catalase with additives may be used for elimination of excess of hydrogen peroxide after bleaching of textile.

1979 ◽  
Vol 25 (7) ◽  
pp. 1267-1273 ◽  
Author(s):  
P C Kao ◽  
N S Jiang ◽  
P C Carpenter

Abstract A corticotropin antiserum was obtained from rabbits immunized with synthetic 1--24 corticotropin conjugated with bovine serum albumin. The antiserum did not cross react with synthetic alpha-melanotropin or with synthetic beta-endorphin and had a cross reactivity of 0.23% with human beta-lipotropin. We developed a radioimmunoassay with the antiserum obtained, in which we used polyethylene glycol in conjunction with a second precipitating antibody for fast (15-min) separation of antibody-bound and free corticotropin. The assay had a sensitivity of 16 ng/L and was validated on patients with various pituitary and adrenal diseases. From 103 normal subjects, the median value for corticotropin in specimens collected during the morning was 34 ng/L of plasma; the upper 95% confidence limit of the normal range was 98 ng/L.


2021 ◽  
pp. 2150028
Author(s):  
Ting Li ◽  
Jialu Yu ◽  
Hao Sui ◽  
Tao Zhang ◽  
Ronghui Zhou

Nanohydroxyapatite (nHAp) has gained considerable concerns due to its vast potential in biomedical applications such as drug delivery, tissue engineering and bone repair. However, the preparation of HAp nanostructures in a controllable manner under environment-friendly reaction conditions remains a challenge. In recent years, the use of biological macromolecules or proteins as templates in the production of nanomaterials has gained more attention due to the relatively mild physical conditions needed for biomimetic synthesis. In this study, a novel nHAp was fabricated by employing bovine serum albumin (BSA) as template under mild condition. After that, the as-obtained nanostructured materials which have well-defined structures and morphologies were characterized by various methods. Furthermore, the rod-like shaped hydroxyapatite demonstrated improved stability properties, as well as cell viability and biocompatibility, compared to BSA free synthesized c-HAp. We expect that this pleasantly novel research will render new insights into the fabrication strategies of nanomaterials and be of practical importance for the expanding biological application.


2013 ◽  
Vol 704 ◽  
pp. 43-50
Author(s):  
Wen Zhi Zhang ◽  
Kuan Zhang ◽  
Jian Long Zheng ◽  
Hong Shu Chen ◽  
Wei Xing Chen ◽  
...  

In order to gain deeper insight into the interaction mechanism between bovine serum albumin (BSA) and polyethylene glycol (PEG), the present work applied elastic light scattering (ELS) spectroscopy to investigate the interaction between BSA and PEG, and explore the effects of concentration and molecular weight of PEG on the interaction at physiological pH. The results showed that the interaction force existed between linear PEG and spherical BSA molecules was mainly hydrogen bonding. In addition, the apparent binding constant of system was evaluated by model calculation.


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