scholarly journals Quantitative Autoradiography

2020 ◽  
Author(s):  
Keyword(s):  
Quantitative Autoradiography

Muscarinic cholinergic receptors in human infant forebrain: [3H]Quinuclidinyl benzilate binding in homogenates and quantitative autoradiography in sections

1985 ◽  
Vol 19 (2) ◽  
pp. 195-203 ◽  
Author(s):  
Michael V. Johnston ◽  
Fayes S. Silverstein ◽  
Fredericko O. Reindel ◽  
John B. Penney ◽  
Anne B. Young
Keyword(s):  
Cholinergic Receptors ◽  
Quantitative Autoradiography ◽  
Human Infant ◽  
Muscarinic Cholinergic Receptors ◽  
Muscarinic Cholinergic ◽  
Quinuclidinyl Benzilate

Intracellular pathway of 125i somatostatin (∗ s14) in isolated guinea pig pancreatic acini : quantitative autoradiography in electron microscopy

1984 ◽  
Vol 20 (6) ◽  
pp. 1604 ◽  
Author(s):  
D. Balas ◽  
F. Senegas-Balas ◽  
C. Susini ◽  
J.P. Estève ◽  
N. Vaysse ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Guinea Pig ◽  
Quantitative Autoradiography ◽  
Pancreatic Acini ◽  
Intracellular Pathway

Ontogenetic development of histamine receptor subtypes in rat brain demonstrated by quantitative autoradiography

1995 ◽  
Vol 87 (2) ◽  
pp. 101-110 ◽  
Author(s):  
Jong Hoon Ryu ◽  
Kazuhiko Yanai ◽  
Eiko Sakurai ◽  
Choong-Yong Kim ◽  
Takehiko Watanabe
Keyword(s):  
Rat Brain ◽  
Histamine Receptor ◽  
Quantitative Autoradiography ◽  
Ontogenetic Development ◽  
Receptor Subtypes

Three-dimensional reconstruction of metabolic data from quantitative autoradiography of rat brain

1984 ◽  
Vol 247 (3) ◽  
pp. E412-E419 ◽  
Author(s):  
L. S. Hibbard ◽  
R. A. Hawkins
Keyword(s):  
High Speed ◽  
Three Dimensional ◽  
Image Data ◽  
Brain Structures ◽  
Three Dimensions ◽  
Quantitative Autoradiography ◽  
Alignment Algorithm ◽  
Computer Methods ◽  
Mathematical Properties ◽  
Dimensional Reconstruction

Quantitative autoradiography is a powerful method for studying brain function by the determination of blood flow, glucose utilization, or transport of essential nutrients. Autoradiographic images contain vast amounts of potentially useful information, but conventional analyses can practically sample the data at only a small number of points arbitrarily chosen by the experimenter to represent discrete brain structures. To use image data more fully, computer methods for its acquisition, storage, quantitative analysis, and display are required. We have developed a system of computer programs that performs these tasks and has the following features: 1) editing and analysis of single images using interactive graphics, 2) an automatic image alignment algorithm that places images in register with one another using only the mathematical properties of the images themselves, 3) the calculation of mean images from equivalent images in different experimental serial image sets, 4) the calculation of difference images (e.g., experiment-minus-control) with the option to display only differences estimated to be statistically significant, and 5) the display of serial image metabolic maps reconstructed in three dimensions using a high-speed computer graphics system.

Characterization of β-adrenergic receptors in sections from human blood lymphocyte pellets by quantitative autoradiography

1988 ◽  
Vol 23 (7) ◽  
pp. 749-754 ◽  
Author(s):  
Masaki Kurihara ◽  
Eero Castrén ◽  
Jorge S. Gutkind ◽  
Kazuto Saito ◽  
Juan M. Saavedra
Keyword(s):  
Human Blood ◽  
Blood Lymphocyte ◽  
Adrenergic Receptors ◽  
Quantitative Autoradiography ◽  
Human Blood Lymphocyte ◽  
Β Adrenergic Receptors

Age-related changes of amino acid receptors in the rat brain studied by quantitative autoradiography

1990 ◽  
Vol 11 ◽  
pp. S107
Author(s):  
Rie Miyoshi ◽  
Shozo Kito ◽  
Naomi Doudou
Keyword(s):  
Amino Acid ◽  
Rat Brain ◽  
Quantitative Autoradiography ◽  
Amino Acid Receptors ◽  
Age Related ◽  
Age Related Changes

scholarly journals [3H]-ouabain binding sites in rat brain: distribution and properties assessed by quantitative autoradiography.

1992 ◽  
Vol 40 (6) ◽  
pp. 771-779 ◽  
Author(s):  
A A Maki ◽  
D G Baskin ◽  
W L Stahl
Keyword(s):  
Nervous System ◽  
Rat Brain ◽  
Cardiac Glycoside ◽  
Binding Sites ◽  
Quantitative Autoradiography ◽  
Affinity Binding ◽  
Ouabain Binding ◽  
High Affinity Binding ◽  
High Affinity ◽  
Kd Values

The anatomic distribution of high- and low-affinity cardiac glycoside binding sites in the nervous system is largely unknown. In the present study the regional distribution and properties of these sites were determined in rat brain by quantitative autoradiography (QAR). Two populations of cardiac glycoside binding sites were demonstrated with [3H]-ouabain, a specific inhibitor of Na,K-ATPases: (a) high-affinity binding sites with Kd values of 22-69 nM, which were blocked by erythrosin B, and (b) low-affinity binding sites with Kd values of 727-1482 nM. Sites with very low affinity for ouabain were not found by QAR. High- and low-affinity [3H]-ouabain binding sites were both found in all brain regions studied, including somatosensory cortex, thalamic and hypothalamic areas, medial forebrain bundle, amygdaloid nucleus, and caudate-putamen, although the distributions of high- and low-affinity sites were not congruent. Low-affinity [3H]-ouabain binding sites (Bmax = 222-358 fmol/mm2) were approximately twofold greater in number than high-affinity binding sites (Bmax = 76-138 fmol/mm2) in these regions of brain. Binding of [3H]-ouabain to both high- and low-affinity sites was blocked by Na+; however, low-affinity binding sites were less sensitive to inhibition by K+ (IC50 = 6.4 mM) than the high-affinity [3H]-ouabain binding sites (IC50 = 1.4 mM). The QAR method, utilizing [3H]-ouabain under standard conditions, is a valid method for studying modulation of Na,K-ATPase molecules in well-defined anatomic regions of the nervous system.

Quantitative autoradiography of β-adrenergic receptors in rabbit eyes

1990 ◽  
Vol 51 (5) ◽  
pp. 503-507 ◽  
Author(s):  
G. Kahle ◽  
P. Kaulen ◽  
J. Wollensak
Keyword(s):  
Adrenergic Receptors ◽  
Quantitative Autoradiography ◽  
Β Adrenergic Receptors
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