scholarly journals Listeria seeligeri

2020 ◽  
Author(s):  
Keyword(s):  
Listeria Seeligeri

Exemplar Abstract for Listeria seeligeri Rocourt and Grimont 1983.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Listeria Seeligeri

scholarly journals Diversity of Listeria Species in Urban and Natural Environments

2012 ◽  
Vol 78 (12) ◽  
pp. 4420-4433 ◽  
Author(s):  
Brian D. Sauders ◽  
Jon Overdevest ◽  
Esther Fortes ◽  
Katy Windham ◽  
Ynte Schukken ◽  
...  
Keyword(s):  
Urban Areas ◽  
Environmental Samples ◽  
Nearest Neighbor ◽  
Allelic Diversity ◽  
Urban Environments ◽  
Source Tracking ◽  
Natural Environments ◽  
Content Type ◽  
Molecular Source ◽  
Listeria Seeligeri

ABSTRACTA total of 442Listeriaisolates, including 234Listeria seeligeri, 80L. monocytogenes, 74L. welshimeri, 50L. innocua, and 4L. marthiiisolates, were obtained from 1,805 soil, water, and other environmental samples collected over 2 years from four urban areas and four areas representing natural environments.Listeriaspp. showed similar prevalences in samples from natural (23.4%) and urban (22.3%) environments. WhileL. seeligeriandL. welshimeriwere significantly associated with natural environments (P≤ 0.0001),L. innocuaandL. monocytogeneswere significantly associated with urban environments (P≤ 0.0001). Sequencing ofsigBfor all isolates revealed 67 allelic types with a higher level of allelic diversity among isolates from urban environments. SomeListeriaspp. andsigBallelic types showed significant associations with specific urban and natural areas. Nearest-neighbor analyses also showed that certainListeriaspp. andsigBallelic types were spatially clustered within both natural and urban environments, and there was evidence that these species and allelic types persisted over time in specific areas. Our data show that members of the genusListerianot only are common in urban and natural environments but also show species- and subtype-specific associations with different environments and areas. This indicates thatListeriaspecies and subtypes within these species may show distinct ecological preferences, which suggests (i) that molecular source-tracking approaches can be developed forListeriaand (ii) that detection of someListeriaspecies may not be a good indicator forL. monocytogenes.

scholarly journals Complete Genome Sequence of Listeria seeligeri, a Nonpathogenic Member of the Genus Listeria

2010 ◽  
Vol 192 (5) ◽  
pp. 1473-1474 ◽  
Author(s):  
Christiane Steinweg ◽  
Carsten T. Kuenne ◽  
André Billion ◽  
Mobarak A. Mraheil ◽  
Eugen Domann ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Listeria Seeligeri

ABSTRACT We report the complete and annotated genome sequence of the nonpathogenic L isteria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria.

Quantitative Evaluation of Three Selective Enrichment Broths and Agars Used in Recovering Listeria Microorganisms

1990 ◽  
Vol 53 (2) ◽  
pp. 105-110 ◽  
Author(s):  
JOSE FERNANDEZ-GARAYZABAL ◽  
CONSTANTIN GEMGEORGIS
Keyword(s):  
Brain Heart Infusion ◽  
Superior Performance ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Naturally Occurring ◽  
Listeria Ivanovii ◽  
Soft Cheese ◽  
Meat Samples ◽  
Listeria Seeligeri ◽  
University Of Vermont

Three selective enrichment broths (FDA, University of Vermont and Dominguez Rodriguez) and lithium chloride-phenylethanol-moxolactam (LPM) agar, modified McBride (MMA) agar, Listeria selective agar (LSAM of Dominguez-Rodriguez), and Brain Heart Infusion (BHI) agar (as reference) were evaluated for their suitability to support the growth of six different species/strains of Listeria (Listeria monocytogenes Scott A, L. monocytogenes V7, L. monocytogenes VPH-1, Listeria innocua, Listeria seeligeri, and Listeria ivanovii). All Listeria strains grew faster and yielded a higher number of cells in FDA enrichment broth. Based on MPN studies, 1 to 2.1 cells of L. monocytogenes and L. innocua were needed for visible colony formation, on all three selective and BHI agars after 48 h incubation of 37°C. The LPM agar was more inhibitory for L. seeligeri and L. ivanovii requiring 9.6 and 917 cells, respectively, as compared to 1 to 2.7 cells for the other agars. The effectiveness of a particular combination among the selective enrichment broths and agars for recovering L. monocytogenes Scott A from inoculated cheese and meat samples was quantitated. Any enrichment broth combined with plating on LPM or LSAM agar gave 100% Listeria recovery as compared to 50 to 67% for plating on MMA agar. Both LPM and LSAM agars have also shown a superior performance to MMA agar in the recovery of naturally occurring Listeria from soft cheese and raw meat. The use of a secondary broth enrichment step improved the recovery of Listeria spp. from meat samples.

Rabbit Meat as a Source of Bacterial Foodborne Pathogens

2006 ◽  
Vol 69 (5) ◽  
pp. 1106-1112 ◽  
Author(s):  
JOSE M. RODRÍGUEZ-CALLEJA ◽  
ISABEL GARCÍA-LÓPEZ ◽  
MARÍA-LUISA GARCÍA-LÓPEZ ◽  
JESÚS A. SANTOS ◽  
ANDRÉS OTERO
Keyword(s):  
Foodborne Pathogens ◽  
Staphylococcal Enterotoxin ◽  
Contamination Rate ◽  
E Coli ◽  
Aeromonas Veronii ◽  
Listeria Ivanovii ◽  
Worldwide Production ◽  
Rabbit Meat ◽  
Listeria Seeligeri ◽  
And Staphylococcus Aureus

Even though worldwide production of rabbit meat is >1,000,000 tons, little information is available for rabbit meat microbiology. This study provides data on the prevalence of Salmonella, Escherichia coli O157:H7, Yersinia enterocolitica, Listeria spp., motile Aeromonas spp., and Staphylococcus aureus on rabbit meat. A total of 24 rabbit carcasses from two abattoirs and 27 rabbit meat packages from supermarket displays were examined. In addition to culturing methods, associated virulence genes were investigated by PCR in suspect isolates and samples. Neither Salmonella nor E. coli O157:H7 was detected. All samples were negative for virulence-associated invA, stx1, and stx2 genes. At one abattoir, two carcasses (3.9%) carried Y. enterocolitica yst−, and two were positive for the yst gene, although viable Y. enterocolitica cells were not recovered from these samples. Seven samples (13.7%) were contaminated with Listeria. Of them, three were positive for hly and iap genes (Listeria monocytogenes hly+/iap+), two carried Listeria seeligeri, one carried Listeria ivanovii, and one carried Listeria innocua. For detectable motile Aeromonas spp. (average count, 1.77 ± 0.62 log CFU/g), the contamination rate was 35.3%, although ca. 90% of the samples were positive for the aerA and/or hlyA genes. The majority of aeromonad isolates were Aeromonas hydrophila aerA+/hlyA+. Aeromonas caviae, Aeromonas popoffii, Aeromonas schubertii, and the two biovars of Aeromonas veronii were also isolated. The prevalence of S. aureus contamination (average count, 1.37 ± 0.79 log CFU/g) was 52.9%. Among 27 S. aureus isolates, two harbored genes for staphylococcal enterotoxin B (seb), and two harbored genes for staphylococcal enterotoxin C (sec). The remaining isolates were negative for sea, seb, sec, sed, and see.

scholarly journals Reservoirs of Listeria Species in Three Environmental Ecosystems

2014 ◽  
Vol 80 (18) ◽  
pp. 5583-5592 ◽  
Author(s):  
Kristina Linke ◽  
Irene Rückerl ◽  
Katharina Brugger ◽  
Renata Karpiskova ◽  
Julia Walland ◽  
...  
Keyword(s):  
Water Samples ◽  
Agricultural Land ◽  
Content Type ◽  
Ecological Sites ◽  
Domestic Ruminants ◽  
Danube Region ◽  
Resistance Rates ◽  
Wildlife Reserve ◽  
Listeria Seeligeri ◽  
Soil And Water

ABSTRACTSoil and water are suggested to represent pivotal niches for the transmission ofListeria monocytogenesto plant material, animals, and the food chain. In the present study, 467 soil and 68 water samples were collected in 12 distinct geological and ecological sites in Austria from 2007 to 2009.Listeriawas present in 30% and 26% of the investigated soil and water samples, respectively. Generally, the most dominant species in soil and water samples wereListeria seeligeri,L. innocua, andL. ivanovii. The human- and animal-pathogenicL. monocytogeneswas isolated exclusively from 6% soil samples in regions A (mountainous region) and B (meadow). Distinct ecological preferences were observed forL. seeligeriandL. ivanovii, which were more often isolated from wildlife reserve region C (Lake Neusiedl) and from sites in proximity to wild and domestic ruminants (region A). The higherL. monocytogenesdetection and antibiotic resistance rates in regions A and B could be explained by the proximity to agricultural land and urban environment.L. monocytogenesmultilocus sequence typing corroborated this evidence since sequence type 37 (ST37), ST91, ST101, and ST517 were repeatedly isolated from regions A and B over several months. A higherL. monocytogenesdetection and strain variability was observed during flooding of the river Schwarza (region A) and Danube (region B) in September 2007, indicating dispersion via watercourses.

Virulence of Listeria monocytogenes Serovars and Listeria spp. in Experimental Infection of Mice

1991 ◽  
Vol 54 (12) ◽  
pp. 917-921 ◽  
Author(s):  
ALAIN MENUDIER ◽  
CLAUDINE BOSIRAUD ◽  
JEAN-ALBERT NICOLAS
Keyword(s):  
Listeria Monocytogenes ◽  
Peritoneal Lavage ◽  
Lavage Fluid ◽  
Fixed Number ◽  
Peritoneal Lavage Fluid ◽  
Listeria Ivanovii ◽  
Wild Strains ◽  
Camp Test ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Wild strains of Listeria monocytogenes, Listeria ivanovii, Listeria seeligeri, Listeria innocua, and Listeria welshimeri were isolated from infected animals and foodstuffs. Their virulence was tested in Swiss mice after intraperitoneal injection of a fixed number of organisms. The presence of hemolysin was determined using the CAMP test. Bacteria were enumerated in peritoneal lavage fluid, liver, and spleen. Spleen weights were measured, and the presence of L. monocytogenes in the brain was also investigated. L. innocua, L. seeligeri, and L. welshimeri were not found to be pathogenic for mice. L. ivanovii was detected in liver, spleen, and peritoneal lavage fluid but at lower levels than L. monocytogenes (p<0.001). The pathogenic capabilities of four different serovars of L. monocytogenes (4b, 1/2a, 1/2b, 1/2c) were compared. Serovars l/2b and l/2c, which are frequently isolated from foodstuffs, were found to colonize the liver and spleen to a lesser extent than serovar 4b (p<0.01 and <0.001 respectively). The behavior of serovar l/2a, the most commonly isolated from foodstuffs, was strain dependent. Two out of the four strains tested were strongly hemolytic and were as virulent as strains of serovar 4b, while the other two were weakly hemolytic, and avirulent like L. innocua. These results could account for the relatively small number of human Listeria infections due to L. monocytogenes serogroup 1/2, despite the very frequent occurrence of this serovar in foodstuffs.

Typing of Epidemiologically Associated Environmental and Clinical Strains of Listeria monocytogenes by Random Amplification of Polymorphic DNA

1995 ◽  
Vol 58 (6) ◽  
pp. 609-613 ◽  
Author(s):  
KEVIN G. KERR ◽  
PETER KITE ◽  
JOHN HERITAGE ◽  
PETER M. HAWKEY
Keyword(s):  
Listeria Monocytogenes ◽  
Rapd Analysis ◽  
Listeria Innocua ◽  
Phage Typing ◽  
Randomly Amplified Polymorphic Dna ◽  
Inexpensive Method ◽  
Clinical Strains ◽  
Random Amplification ◽  
Listeria Seeligeri ◽  
Retail Food

One hundred and three strains of Listeria monocytogenes(Lm), Listeria seeligeri and Listeria innocua of clinical, food, and environmental origin were examined by generating randomly amplified polymorphic DNA (RAPD). Using one 10 bp and two 13 bp random primers, epidemiologically related strains, previously shown to be indistinguishable by phage typing, yielded identical RAPD profiles. Strains isolated from the hands of three workers in a retail food establishment showed the presence of a single predominant Lm isolate. RAPD analysis is a rapid, reproducible, and relatively inexpensive method for the differentiation of epidemiologically linked isolates of Listeria spp.

Nomenclature Abstract for Listeria seeligeri Rocourt and Grimont 1983.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Listeria Seeligeri
Sign in / Sign up

Export Citation Format

Share Document