scholarly journals Reservoirs of Listeria Species in Three Environmental Ecosystems

2014 ◽  
Vol 80 (18) ◽  
pp. 5583-5592 ◽  
Author(s):  
Kristina Linke ◽  
Irene Rückerl ◽  
Katharina Brugger ◽  
Renata Karpiskova ◽  
Julia Walland ◽  
...  

ABSTRACTSoil and water are suggested to represent pivotal niches for the transmission ofListeria monocytogenesto plant material, animals, and the food chain. In the present study, 467 soil and 68 water samples were collected in 12 distinct geological and ecological sites in Austria from 2007 to 2009.Listeriawas present in 30% and 26% of the investigated soil and water samples, respectively. Generally, the most dominant species in soil and water samples wereListeria seeligeri,L. innocua, andL. ivanovii. The human- and animal-pathogenicL. monocytogeneswas isolated exclusively from 6% soil samples in regions A (mountainous region) and B (meadow). Distinct ecological preferences were observed forL. seeligeriandL. ivanovii, which were more often isolated from wildlife reserve region C (Lake Neusiedl) and from sites in proximity to wild and domestic ruminants (region A). The higherL. monocytogenesdetection and antibiotic resistance rates in regions A and B could be explained by the proximity to agricultural land and urban environment.L. monocytogenesmultilocus sequence typing corroborated this evidence since sequence type 37 (ST37), ST91, ST101, and ST517 were repeatedly isolated from regions A and B over several months. A higherL. monocytogenesdetection and strain variability was observed during flooding of the river Schwarza (region A) and Danube (region B) in September 2007, indicating dispersion via watercourses.

2021 ◽  
Vol 16 (2) ◽  
pp. 477-491
Author(s):  
Subramanian Arivoli ◽  
Miriam Vassou ◽  
Samuel Tennyson ◽  
Athikesavan Ramanan ◽  
Selvaraj Divya ◽  
...  

Quality of soil and water are determined by measuring the concentration of their parameters and comparing them with standards. In the present study, soil samples (top, sub and inner) from three zones, zone 1 (industrial area), zone 2 (10km from industrial area) and zone 3 (agricultural land/fields) of Puliyanthangal village, and the bore well, well and pond water samples of Kathiyavadi village were analysed from January 2019 to December 2019. Soil texture was sandy loam in all the zones. The pooled values for pH, electrical conductivity (dS/m), organic carbon (%), available nitrogen, phosphorous, potassium, iron, manganese, zinc and copper (mg/Kg) tested in zone 1, 2 and 3 were 7.9, 6.8 and 6.8; 0.3, 0.3 and 0.1; 0.09, 0.5 and 0.5; 11.7, 96.0 and 137.8; 4.5, 4.5 and 4.2; 88.3, 111.3 and 206.7; 3.6, 3.4 and 3.7; 2.1, 1.7 and 2.3; 0.4, 0.3 and 0.2; and 0.7, 0.5 and 0.7, respectively. The bore well and well water samples were clear and colourless without odour, whereas the pond water was slightly yellowish in appearance and colour, and without odour in all the three zones. The physicochemical parameters viz., water temperature, electrical conductivity, turbidity, total dissolved solids, pH, total alkalinity and total hardness for bore well, well and pond water were 27.9, 22.8 and 33.0°C; 975, 1532 and 737μS/cm; 0, 1 and 11NTU; 683, 1072 and 516mg/L; 7.4, 7.8 and 7.3; 276, 344 and 248mg/L; and 190, 732 and 272mg/L, respectively. Calcium, magnesium, free ammonia, nitrate, nitrite, chloride, fluoride, sulphate and phosphate represented the nutrient parameters and their respective values (mg/L) were 41, 174 and 58; 21, 71 and 30; 0, 0 and 1.1; 24, 29 and 22; 0, 0 and 0.8; 100, 184 and 60; 0.4, 0.4 and 0.4; 77, 120 and 49; 0, 0 and 0.8. The values of metal parameters reported nil except for iron (0.2mg/L) in pond water. The study inferred that the waning nature of soil and water might be due to location of industrial units.


Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 698
Author(s):  
Sónia Gomes ◽  
Conceição Fernandes ◽  
Sandra Monteiro ◽  
Edna Cabecinha ◽  
Amílcar Teixeira ◽  
...  

The inappropriate use of antibiotics, one of the causes of the high incidence of antimicrobial-resistant bacteria isolated from aquatic ecosystems, represents a risk for aquatic organisms and the welfare of humans. This study aimed to determine the antimicrobial resistance rates among riverine Aeromonas spp., taken as representative of the autochthonous microbiota, to evaluate the level of antibacterial resistance in the Tua River (Douro basin). The prevalence and degree of antibiotic resistance was examined using motile aeromonads as a potential indicator of antimicrobial susceptibility for the aquatic environment. Water samples were collected from the middle sector of the river, which is most impacted area by several anthropogenic pressures. Water samples were plated on an Aeromonas-selective agar, with and without antibiotics. The activity of 19 antibiotics was studied against 30 isolates of Aeromonas spp. using the standard agar dilution susceptibility test. Antibiotic resistance rates were fosfomycin (FOS) 83.33%, nalidixic acid (NA) 60%, cefotaxime (CTX) 40%, gentamicin (CN) 26.67%, tobramycin (TOB) 26.67%, cotrimoxazole (SXT) 26.67%, chloramphenicol (C) 16.67%, and tetracycline (TE) 13.33%. Some of the nalidixic acid-resistant strains were susceptible to fluoroquinolones. Multiple resistance was also observed (83.33%). The environmental ubiquity, the natural susceptibility to antimicrobials and the zoonotic potential of Aeromonas spp. make them optimal candidates for studying antimicrobial resistance (AMR) in aquatic ecosystems. Aquatic environments may provide an ideal setting for the acquisition and dissemination of antibiotic resistance because anthropogenic activities frequently impact them. The potential risk of multi- and pan-resistant bacteria transmission between animals and humans should be considered in a “One Health—One World” concept.


2012 ◽  
Vol 78 (12) ◽  
pp. 4420-4433 ◽  
Author(s):  
Brian D. Sauders ◽  
Jon Overdevest ◽  
Esther Fortes ◽  
Katy Windham ◽  
Ynte Schukken ◽  
...  

ABSTRACTA total of 442Listeriaisolates, including 234Listeria seeligeri, 80L. monocytogenes, 74L. welshimeri, 50L. innocua, and 4L. marthiiisolates, were obtained from 1,805 soil, water, and other environmental samples collected over 2 years from four urban areas and four areas representing natural environments.Listeriaspp. showed similar prevalences in samples from natural (23.4%) and urban (22.3%) environments. WhileL. seeligeriandL. welshimeriwere significantly associated with natural environments (P≤ 0.0001),L. innocuaandL. monocytogeneswere significantly associated with urban environments (P≤ 0.0001). Sequencing ofsigBfor all isolates revealed 67 allelic types with a higher level of allelic diversity among isolates from urban environments. SomeListeriaspp. andsigBallelic types showed significant associations with specific urban and natural areas. Nearest-neighbor analyses also showed that certainListeriaspp. andsigBallelic types were spatially clustered within both natural and urban environments, and there was evidence that these species and allelic types persisted over time in specific areas. Our data show that members of the genusListerianot only are common in urban and natural environments but also show species- and subtype-specific associations with different environments and areas. This indicates thatListeriaspecies and subtypes within these species may show distinct ecological preferences, which suggests (i) that molecular source-tracking approaches can be developed forListeriaand (ii) that detection of someListeriaspecies may not be a good indicator forL. monocytogenes.


Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mulayam Singh Gaur ◽  
Rajni Yadav ◽  
Mamta Kushwah ◽  
Anna Nikolaevna Berlina

Purpose This information will be useful in the selection of materials and technology for the detection and removal of mercury ions at a low cost and with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. Design/methodology/approach Different nano- and bio-materials allowed for the development of a variety of biosensors – colorimetric, chemiluminescent, electrochemical, whole-cell and aptasensors – are described. The materials used for their development also make it possible to use them in removing heavy metals, which are toxic contaminants, from environmental water samples. Findings This review focuses on different technologies, tools and materials for mercury (heavy metals) detection and remediation to environmental samples. Originality/value This review gives up-to-date and systemic information on modern nanotechnology methods for heavy metal detection. Different recognition molecules and nanomaterials have been discussed for remediation to water samples. The present review may provide valuable information to researchers regarding novel mercury ions detection sensors and encourage them for further research/development.


2015 ◽  
Vol 60 (3) ◽  
pp. 1500-1508 ◽  
Author(s):  
R. H. Jensen ◽  
H. K. Johansen ◽  
L. M. Søes ◽  
L. E. Lemming ◽  
F. S. Rosenvinge ◽  
...  

The prevalence of intrinsic and acquired resistance among colonizingCandidaisolates from patients after candidemia was investigated systematically in a 1-year nationwide study. Patients were treated at the discretion of the treating physician. Oral swabs were obtained after treatment. Species distributions and MIC data were investigated for blood and posttreatment oral isolates from patients exposed to either azoles or echinocandins for <7 or ≥7 days. Species identification was confirmed using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and internal transcribed spacer (ITS) sequencing, susceptibility was examined by EUCAST EDef 7.2 methodology, echinocandin resistance was examined byFKSsequencing, and genetic relatedness was examined by multilocus sequence typing (MLST). One hundred ninety-three episodes provided 205 blood and 220 oral isolates. MLST analysis demonstrated a genetic relationship for 90% of all paired blood and oral isolates. Patients exposed to azoles for ≥7 days (n= 93) had a significantly larger proportion of species intrinsically less susceptible to azoles (particularlyCandida glabrata) among oral isolates than among initial blood isolates (36.6% versus 12.9%;P< 0.001). A similar shift toward species less susceptible to echinocandins among 85 patients exposed to echinocandins for ≥7 days was not observed (4.8% of oral isolates versus 3.2% of blood isolates;P> 0.5). Acquired resistance inCandida albicanswas rare (<5%). However, acquired resistance to fluconazole (29.4%;P< 0.05) and anidulafungin (21.6%;P< 0.05) was common inC. glabrataisolates from patients exposed to either azoles or echinocandins. Our findings suggest that the colonizing mucosal microbiota may be an unrecognized reservoir of resistantCandidaspecies, especiallyC. glabrata, following treatment for candidemia. The resistance rates were high, raising concern in general for patients exposed to antifungal drugs.


1994 ◽  
Vol 149 (3) ◽  
pp. 217-222 ◽  
Author(s):  
Bernard Paul ◽  
Philippe Bouchet ◽  
Françoise Bouchet

Author(s):  
I. Sh. Normatov ◽  
V.V. Goncharuk ◽  
N.A. Amirgaliev ◽  
A.S. Madibekov ◽  
A.I. Normatov

The water quality of the transboundary Pyanj River in the formation zone and along the riverbed before merging with another tributary of the transboundary Amu Darya River-the Vakhsh River was studied. The water quality on the upstream river corresponds to the very soft class (> 1.5 mmol/dm3) and in the middle and the downstream to the soft class (1.5-3.0 mmol/dm3). At the upper, middle and lower reaches of the Pyanj river the concentration of alkaline earth exceeds alkali metals (Ca2+ + Mg2+> Na+ + K+) at HCO3- > SO42- + Cl- and according to the Handa classification they are characterized by temporary rigidity. To assess the criterion of applicability of the Pyanj river water for irrigation the coefficient of sodium adsorption (SAC) was calculated for water samples from the upstream (Khorog), middle (Darvaz) and the downstream (Lower Pyanj) of the Pyanj river that were equal to 0.88; 1.07; 1.71, respectively. The SAC values for all water samples (from the upper, middle and lower reaches) of the Pyanj river indicate their good qualities for irrigation of agricultural land. The concentration of heavy metals in the Pyanj river is significantly lower than the maximum permissible concentration (MPC).


2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Mamush Masha ◽  
Teshome Yirgu ◽  
Mulugeta Debele ◽  
Mengie Belayneh

Soil and water conservation (SWC) is being advocated as an integral part of agricultural land management as it not only controls/minimizes soil erosion but also restores/rehabilitates the degraded lands. The purpose of this study was to evaluate the impact of soil and water conservation practices in improving soil fertility in the agricultural landscapes of the Damota area, southern Ethiopia. Forty-eight soil samples (both disturbed and core samples) were collected from the conserved and adjacent nonconserved plots. The significance analysis test was performed using analysis of variance. The result of the study showed that higher mean values of soil physicochemical properties were observed in the conserved plot than its nonconserved counterpart. The mean differences of organic carbon, total nitrogen, cation exchange capacity, and exchangeable K+ and Ca2+ between conserved and nonconserved plots were statistically significant at the P < 0.01 level. Besides, available phosphorous and bulk density were significant at P < 0.05 , but the effect of SWC practices was not found significant on soil texture, soil pH, and exchangeable Na+ and Mg2+ content of the soil in the Damota area. Community-based soil and water conservation practices have improved the soil fertility in agricultural landscapes, although significant results have been observed in some fertility indicators. Therefore, strengthening the implementation of conservation measures by participating in all stakeholders is recommended. Supporting physical structures by agronomic and vegetative measures and continued maintenance can bring better results.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Kafferine Yamagishi ◽  
Cecil Gantalao ◽  
Lanndon Ocampo

Purpose This study aims to draw observations on the current status and potentials of the Philippines as a farm tourism destination and identify the underlying factors that inhibit farm tourism development. It intends to gauge the challenges that Filipino farmers face in diversifying farms and operating farm sites and uses these challenges in crafting strategies and policies for relevant stakeholders. It also provides Philippine farm tourism literature to address the limitations of references in the topic. Design/methodology/approach The study adopts an exploratory type of inquiry method and secondary data collection from various sources, such as published journal articles, news articles and reports, to gain insights and relevant information on farm tourism. The study also uses a threats, opportunities, weaknesses and strengths analysis approach to develop competitive farm tourism strategies. Findings The Philippines, with vast agricultural land, has the necessary base for farm tourism, and the enactment of the Farm Tourism Development Act of 2016 bridges this potential. With low agricultural outputs, the country draws relevance for farm tourism as a farm diversification strategy to supplement income in rural communities. While having these potentials, crucial initiatives in physical characteristics, product development, education and training, management and entrepreneurship, marketing and customer relations and government support must be implemented. Farmers' lack of skills, training and capital investment potential to convert their farms into farm tourism sites serves as the major drawback. Thus, developing entrepreneurial and hospitality skills is crucial. Originality/value This work presents a historical narrative of initiatives and measures of the Philippine farm tourism sector. It also provides a holistic discussion and in-depth analysis of the current state, potentials, strategies and forward insights for farm tourism development.


2013 ◽  
Vol 37 (6) ◽  
pp. 1431-1440 ◽  
Author(s):  
Tiago Santos Telles ◽  
Sonia Carmela Falci Dechen ◽  
Maria de Fátima Guimarães

The problem of soil erosion in Brazil has been a focus of agricultural scientific research since the 19th century. The aim of this study was to provide a historical overview of the institutional landmarks which gave rise to the first studies in soil erosion and established the foundations of agricultural research in Brazil. The 19th century and beginning of the 20th century saw the founding of a series of institutions in Brazil, such as Botanical Gardens, executive institutions, research institutes, experimental stations, educational institutions of agricultural sciences, as well as the creation and diversification of scientific journals. These entities, each in its own way, served to foster soil erosion research in Brazil. During the Imperial period (1808-1889), discussions focused on soil degradation and conserving the fertility of agricultural land. During the First Republic (1889-1930), with the founding of various educational institutions and consolidation of research on soil degradation conducted by the Agronomic Institute of Campinas in the State of São Paulo, studies focused on soil depletion, identification of the major factors causing soil erosion and the measures necessary to control it. During the New State period (1930-1945), many soil conservation practices were developed and disseminated to combat erosion and field trials were set up, mainly to measure soil and water losses induced by hydric erosion. During the Brazilian New Republic (1945-1964), experiments were conducted throughout Brazil, consolidating soil and water conservation as one of the main areas of Soil Science in Brazil. This was followed by scientific conferences on erosion and the institutionalization of post-graduate studies. During the Military Regime (1964-1985), many research and educational institutions were founded, experimental studies intensified, and coincidently, soil erosion reached alarming levels which led to the development of the no-tillage system.


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