scholarly journals Flow Cytometry and Sperm Sexing in Animals

2018 ◽  
Vol 3 (3) ◽  
pp. 893-902
Author(s):  
Paulo Salinas
Keyword(s):  
Flow Cytometry ◽  
Light Beam ◽  
Fluorescence Intensity ◽  
Dna Content ◽  
Artificial Insemination ◽  
Stream Flow ◽  
Relative Fluorescence Intensity ◽  
Flow Through ◽  
Accuracy Speed ◽  
Current Procedure

Flow cytometry is a useful technology in the sexed sperm, which measures and analyzes simultaneously, multiple physical characteristics of the cell, as they flow in a stream flow, through a light beam. The measured properties are the size of a particle, relative internal granularity, relative complexity and relative fluorescence intensity. Currently, hundreds of calves have been gestated through artificial insemination with sexed sperm in animal production. Since 1992, flow cytometry has been used, a technique that allows spermatozoa X and Y differentiation by DNA content. There is no other practical technique for sperm sexing to keep sperm functionality. The objectives of this review are to explain: (1) why the sperm containing the X or Y chromosome are phenotypically similar, but differ among themselves, (2) the principles and procedures used for sexing sperm by flow cytometry and sorting ( 3) accuracy, speed and efficiency of current procedure sperm sexing, (4) sperm damage occurred during sperm sexing and consequently the effects on fertility.

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

scholarly journals Brief communication. First evaluation of nuclear DNA content in Setaria genus by flow cytometry

1998 ◽  
Vol 89 (6) ◽  
pp. 556-559 ◽  
Author(s):  
M Le Thierry d'Ennequin ◽  
O Panaud ◽  
S Brown ◽  
S Siljak-Yakovlev ◽  
A Sarr
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

scholarly journals Touch Imprint Intraoperative Flow Cytometry as a Complementary Tool for Detailed Assessment of Resection Margins and Tumor Biology in Liver Surgery for Primary and Metastatic Liver Neoplasms

2021 ◽  
Vol 4 (3) ◽  
pp. 66
Author(s):  
Georgios S. Markopoulos ◽  
Georgios K. Glantzounis ◽  
Anna C. Goussia ◽  
Georgios D. Lianos ◽  
Anastasia Karampa ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Liver Tumors ◽  
Tumor Biology ◽  
Surgical Margins ◽  
Distribution Analysis ◽  
Cell Analysis ◽  
Term Survival ◽  
Detailed Assessment ◽  
Metastatic Liver

Liver resection is the main treatment for primary and metastatic liver tumors in order to achieve long-term survival with good quality of life. The ultimate goal of surgical oncology is to achieve complete tumor removal with adequate clear surgical margins. Flow cytometry is a powerful analytical technique with applications such as phenotypic analysis and quantification of DNA content. Intraoperative flow cytometry (iFC) is the application of flow cytometry for DNA content/ploidy and cell cycle distribution analysis during surgery for tumor cell analysis and margin evaluation. It has been used for cell analysis of intracranial tumors and recently of head and neck carcinomas and breast carcinomas, as well as for tumor margin evaluation. Herein, we present a novel touch imprint iFC protocol for the detailed assessment of tumor margins during excision of malignant hepatic lesions. The protocol aims to offer information on surgical margins after removal of malignant liver tumors based on DNA content of cancer cells and to corroborate the results of iFC with that of histopathological analysis. Based on the established role of iFC in other types of malignancies, our specialized protocol has the potential, through characterization of cells in liver transection surface post hepatectomy, to offer significant information on the type of resection and tumor biology. This information can be used to effectively guide intra- and postoperative patient management.

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