scholarly journals STUDY ON THE CAPABILITY OF BIO-BUTANOL SYNTHESIS FROM SUGARCANE BAGASSE

2011 ◽  
Vol 14 (3) ◽  
pp. 87-96
Author(s):  
Quyen Huynh ◽  
Tuan Dinh Phan
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Steam Explosion ◽  
Cellulose Hydrolysis ◽  
Clostridium Beijerinckii ◽  
Main Concern ◽  
Cellulose Content ◽  
Glucose Yield ◽  
Cellulose Accessibility ◽  
Acetone Butanol Ethanol

The main concern in converting sugarcane bagasse to bio-butanol fuel is the conversion of the polysaccharides by enzymatic breakdown into monosaccharides. This study focused on the use of steam explosion as a pretreatment method. Steam explosion treatment of biomass had been previously used to increase cellulose accessibility [1,3,5,6,8,9]. Following steam explosion pretreatment, sugarcane bagasse was subjected to enzymatic hydrolysis employing the Acremonium Cellulase as the reactant. The sugars released by enzymatic hydrolysis were further fermented by Clostridium Beijerinckii. Raw sugarcane bagasse was found to have the polysaccharides content of 56.24%. The fiber loss during the steam explosion treatment was high, up to 67.11%. Steam explosion treatment on sugarcane bagasse increased the enzymatic hydrolysis capability of cellulose. After steam explosion treatment at temperature of 224oC for 2 minutes, the cellulose hydrolysis conversion efficiency could reach 98.04% by applying Acremonium Cellulase for 72 hours. It has been stated that steam explosion was suitable to improve cellulose content and consequently improve fermentable glucose yield from enzymatic hydrolysis while drastically reducing hemicellulose content of the fibers. Butanol has been successfully produced from the sugarcane bagasse hydrolysate in acetone-butanol-ethanol (ABE) process applying C. Beijerinckii.

scholarly journals Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 507
Author(s):  
Eduardo Troncoso-Ortega ◽  
Rosario del P. Castillo ◽  
Pablo Reyes-Contreras ◽  
Patricia Castaño-Rivera ◽  
Regis Teixeira Mendonça ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Eucalyptus Globulus ◽  
Steam Explosion ◽  
Laser Scanning ◽  
Structural Changes ◽  
Micro Particles ◽  
Cellulose Accessibility ◽  
Ft Ir ◽  
Enzymatic Accessibility ◽  
Physical And Chemical

The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose.

scholarly journals Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4552 ◽  
Author(s):  
Merve Nazli Borand ◽  
Asli Isler Kaya ◽  
Filiz Karaosmanoglu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Liquid Fraction ◽  
Sugar Content ◽  
Release Time ◽  
Variable Parameters ◽  
Glucose Yield ◽  
Steam Explosion Pretreatment ◽  
Saccharification Yield ◽  
Hydrolysis Of

Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

Multifaceted characterization of sugarcane bagasse under different steam explosion severity conditions leading to distinct enzymatic hydrolysis yields

2019 ◽  
Vol 139 ◽  
pp. 111542 ◽  
Author(s):  
Melissa Cristina do Espírito Santo ◽  
Eliano Brito Cardoso ◽  
Francisco Eduardo Gontijo Guimaraes ◽  
Eduardo Ribeiro deAzevedo ◽  
Giovanni Paro da Cunha ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Steam Explosion

scholarly journals Sugars Production from Municipal Forestry and Greening Wastes Pretreated by an Integrated Steam Explosion-Based Process

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4432 ◽  
Author(s):  
Maria José Negro ◽  
Cristina Álvarez ◽  
Pablo Doménech ◽  
Raquel Iglesias ◽  
Ignacio Ballesteros
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Dilute Acid ◽  
Waste Streams ◽  
Enzyme Loading ◽  
Glucose Yield ◽  
Resource Sustainability ◽  
Solids Loading ◽  
Biological Conversion ◽  
Pretreatment Conditions

Increasing awareness of resource sustainability and waste management has led to the search for solutions while promoting circular economy principles. Among all kinds of lignocellulosic biomass available, one with growing interest is municipal forestry and greening waste (MFGW). MFGW makes up an important part of waste streams of municipal solid waste and is a potential feedstock for biological conversion in a lignocellulosic biorefinery. This work studied the fermentable sugars production from MFGW after steam explosion (SE) pretreatment combined with other pretreatments such as dilute acid, organosolv, and metal salts. A range of pretreatment conditions was evaluated according to different parameters: sugars recovery, degradation product generation, and enzymatic hydrolysis yield. At selected pretreatment conditions (diluted acid plus SE, 195 °C, 10 min, and 60 mg H2SO4/g MFGW), 77% of potential sugars content in MFGW was obtained. The effect of solids loading and enzyme dose on glucose release and glucose yield on enzymatic hydrolysis were also determined. Up to 70% of the main sugars in the MFGW were recovered for the coupled pretreatment and enzymatic hydrolysis (45 FPU/g glucan enzyme loading and 20% dry matter solid consistency), resulting in 80 g/L glucose that could be further utilized for ethanol production.

scholarly journals Steam explosion of wood particles from fibreboard and particle board with indirect control by enzymatic hydrolysis

2019 ◽  
Vol 12 (2) ◽  
pp. 185-191
Author(s):  
Andrej Pažitný
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Lignocellulosic Materials ◽  
Particle Board ◽  
Severity Factor ◽  
Cellulose Accessibility ◽  
Indirect Control ◽  
Commercial Enzymes ◽  
Wood Particles ◽  
Pretreatment Conditions

Abstract Pretreatment of particles obtained from lignocellulosic materials by steam explosion with indirect control by enzymatic hydrolysis has been studied. The dendromass pretreatment model has been applied for recycled fibreboard and particle board based on softwood. Their structure and chemical composition partly predetermine these lignocellulosic materials consisting of a mixture of spruce and fir particles also for bioethanol production. Optimum steam explosion temperature of 205 °C was determined based on the concentration of total monosaccharides — glucose, xylose and arabinose, among all experimentally prepared hydrolysates. This corresponds to basic conditions for fine disintegration of biomass to lignocellulosic structure with good holocellulose accessibility. Particles obtained from fibreboard and particle board primarily consisting of softwood without steam explosion pretreatment provide relatively low cellulose accessibility for commercial enzymes activity while monosaccharides concentration is partly reduced because of torrefaction at high temperatures. The concentration of monosaccharides in hydrolysates was determined for original sample and each steam explosion temperature. Based on the steam explosion conditions, the effect of severity factors was investigated to find optimum pretreatment conditions to increase accessibility of softwood cellulose and hemicelluloses. The identified optimum severity factor RO = 4.09 matches the optimum steam explosion temperature of 205 °C and the residence time of 10 minutes.

Synergetic Physical Freezing and Chemical Pretreatment of Lignocellulosic Sugarcane Bagasse Followed by Enzymatic Hydrolysis

2021 ◽  
Author(s):  
Ahmed M. Farghaly ◽  
Kareman Ahmed ◽  
Ali Gad
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Statistical Modelling ◽  
Glucose Yield ◽  
Physical Fractionation ◽  
Chemical Hydrolysis ◽  
Low Glucose ◽  
Total Reducing Sugars ◽  
Predicted Values ◽  
Pretreatment Phase

Abstract This study focuses on employing an eco-friendly pretreatment approach for lignocellulosic Sugarcane Bagasse (SCB) as a major problematic solid waste in Egypt, complying with the standard legislation as well. The applied technique depended on SCB physical fractionation via freezing, followed by chemical hydrolysis using alkaline hydrogen peroxide (AHP) and enzymatic hydrolysis. The changes occurred in macrostructure and the entire lignocellulosic compounds during the pretreatment stages were evaluated. Freezing fractionation resulted in relatively low glucose yield and saccharification ratio at -20°C for 2 h of 307.52 mg/gm native SCB and 48.5%, respectively, where no total reducing sugars (TRS) was obtained. Further AHP pretreatment was performed for the frozen-fractionated SCB at -20°C and 2 h with assistance of Box–Behnken Design response surface methodology (RSM). The investigated key parameters were H2O2 concentration (3, 5.5 and 8 %v/v), temperature (25, 42.5 and 60°C) and pretreatment duration (1, 3 and 5 h). The results revealed that the statistical modelling was able to predict the response of glucose yield and TRS production with R2 = 0.8221 and 0.8814, respectively. Applying the optimization tool of RSM, the optimum predicted values of glucose yield and TRS production were (886.51 mg/gm native SCB and 1.44 mg/mL), respectively; confirmed by the experimental analysis (898.5 mg/gm native SCB and 1.32 mg/mL), respectively. The coincided saccharification ratio was 97.5%. These results were obtained at H2O2 of 3 % (v/v), 56.93°C and 1 h which were 4.32 and 2.01 times higher than that obtained during the freezing pretreatment phase for glucose yield and saccharification ratio, respectively.

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