scholarly journals Effect of thidiazuron and monochromatic light on shoot development in vitro culture of Polygonum cuspidatum Sieb et Zucc

2020 ◽  
Vol 4 (2) ◽  
pp. First
Author(s):  
Doan Phan Phuong Thao ◽  
Tuan Anh Le ◽  
Phan Ngo Hoang
Keyword(s):  
Blue Light ◽  
Phenolic Content ◽  
Monochromatic Light ◽  
Total Phenolic ◽  
Fluorescent Light ◽  
Polygonum Cuspidatum ◽  
Blue Led ◽  
Red Led ◽  
Number Of Shoots

Polygonum cuspidatum Sieb. et Zucc, a medicinal plant, contains many phenolic compounds such as resveratrol. It has antioxidant, antibacterial, anti-cancer, HIV, and neuron protective properties. In this study, stem segments having an auxiliary bud were cultured in vitro on MS medium supplemented with TDZ at 0.1 mg/L for obtaining the highest number of shoots (8–9 shoots/cluster after eight weeks). The bud shoots were originated from the differentiation in the periphery of the lateral meristem. After eight weeks, the shoots cultured under the monochromatic light showed that the plant height, fresh and dry weight was higher than those grown under the fluorescent light at the same intensity of 50 µmol photon/m2/sec. Blue LED reduced the number of shoots/cluster, and respiration intensity of the inoculum compared to the red LED or fluorescent light conditions. However, after eight weeks, the total sugar and phenolic content in leaves and stems, as well as the resveratrol content of shoots under blue light, were always higher than under red LED or fluorescent light. Moreover, when samples were exposed under the one-week blue light condition at a double intensity (100 µmol photon/m2/sec), the shoot clusters growing under blue LED also had a remarkably high total phenolic content and significantly high resveratrol levels compared to the two other treatments. The roles of endogenous growth regulators in shoot clusters under different lighting conditions were also analyzed and discussed.

FLUORESCENT LIGHT (FL), RED LED AND BLUE LED SPECTRUMS EFFECTS ON IN VITRO SHOOTS MULTIPLICATION

2016 ◽  
Vol 78 (6-6) ◽  
Author(s):  
Nurul Syahirah Azmi ◽  
Robiah Ahmad ◽  
Rusli Ibrahim
Keyword(s):  
Large Scale ◽  
Ornamental Plants ◽  
Fruit Production ◽  
Fluorescent Light ◽  
Alternative Source ◽  
Light Emitting ◽  
Blue Led ◽  
In Vitro Shoots ◽  
Red Led

Tissue culture in ornamental plants is one of the relevant factors that beat production of vegetables and fruit production worldwide. It has been recognized as an effective tool to enhance large scale of plant multiplication. However, the conventional lighting system may contain unnecessary wavelength that are low quality to promote growth. In this study, experiment was conducted by using Light Emitting Diodes (LED) as an alternative source of lighting. Red and blue LEDs along with fluorescent light (FL) were applied to determine the best source of light in multiplication of rose. Under the same media regimes which are MS media basal and BAP shoot hormone, blue LED had shown more shoots and leaves. 

scholarly journals Delivery of Phenolic Compounds, Peptides and β-Glucan to the Gastrointestinal Tract by Incorporating Dietary Fibre-Rich Mushrooms into Sorghum Biscuits

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1812
Author(s):  
Juncai Tu ◽  
Margaret Anne Brennan ◽  
Gang Wu ◽  
Weidong Bai ◽  
Ping Cheng ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Dietary Fibre ◽  
Lentinula Edodes ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
In Vitro Digestion ◽  
Total Phenolic ◽  
Soluble Peptide ◽  
Insoluble Dietary Fibre

Sorghum biscuits were enriched with mushroom powders (Lentinula edodes, Auricularia auricula and Tremella fuciformis) at 5%, 10% and 15% substitution levels. An in vitro gastrointestinal digestion was used to evaluate the effect of this enrichment on the phenolic content and soluble peptide content as well as antioxidant activities of the gastric or intestinal supernatants (bio-accessible fractions), and the remaining portions of phenolic compounds, antioxidants and β-glucan in the undigested residue (non-digestible fraction). The phenolic content of the gastric and intestinal supernatants obtained from digested mushroom-enriched biscuits was found to be higher than that of control biscuit, and the phenolic content was positively correlated to the antioxidant activities in each fraction (p < 0.001). L. edodes and T. fuciformis enrichment increased the soluble protein content (small peptide) of sorghum biscuits after in vitro digestion. All mushroom enrichment increased the total phenolic content and β-glucan content of the undigested residue and they were positively correlated (p < 0.001). The insoluble dietary fibre of biscuits was positively correlated with β-glucan content (p < 0.001) of undigested residue. These findings suggested that enriching food with mushroom derived dietary fibre increases the bioavailability of the non-digestible β-glucan and phenolic compounds.

scholarly journals Virulence of Aerial Conidia of Beauveria bassiana Produced under LED Light to Ctenocephalides felis (Cat Flea)

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Sarayut Pittarate ◽  
Malee Thungrabeab ◽  
Supamit Mekchay ◽  
Patcharin Krutmuang
Keyword(s):  
Beauveria Bassiana ◽  
Adult Mortality ◽  
Ctenocephalides Felis ◽  
Fluorescent Light ◽  
Malt Extract ◽  
Led Light ◽  
Red Led ◽  
Aerial Conidia ◽  
Different Color

Ctenocephalides felis is an ectoparasitic flea species commonly found on dogs and cats. The current study verified the in vitro virulence of conidia of the entomopathogenic fungus Beauveria bassiana produced under different color LED light (red, blue, purple, green, yellow, and white) to adults of C. felis. The fungal isolates were cultivated on malt extract agar (MEA). Bioassay treatments used aerial conidia in test tubes. Adult fleas were obtained from a house cat in Chiang Mai province, Thailand. The experiments were composed of one control and eleven treatment groups. All of the treatments with B. bassiana conidia caused adult mortality after an exposure of 12 h. Among the conditions used in this study, B. bassiana cultured under red LED and fluorescent light were the most effective in causing mortality (100 %) in adult fleas after 36 h. The experimental results indicate that these aerial conidia of B. bassiana have promising potential for use in control of C. felis adult stages.

scholarly journals An RNA-Seq Analysis of Grape Plantlets Grown in vitro Reveals Different Responses to Blue, Green, Red LED Light, and White Fluorescent Light

2017 ◽  
Vol 8 ◽  
Author(s):  
Chun-Xia Li ◽  
Zhi-Gang Xu ◽  
Rui-Qi Dong ◽  
Sheng-Xin Chang ◽  
Lian-Zhen Wang ◽  
...  
Keyword(s):  
Fluorescent Light ◽  
Rna Seq ◽  
Led Light ◽  
Red Led

Effect of 1-MCP on Bioavailability of Lingwu Long Jujube In Vitro Digestion

2012 ◽  
Vol 554-556 ◽  
pp. 1357-1360
Author(s):  
Zhong Li Jiang ◽  
Ai Li Wang ◽  
Xi Hong Li ◽  
Min Peng Zhu ◽  
Jun Wei Wang
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Control Samples

The present study investigated the effect of 1-MCP on bioavailability of Lingwu long jujube stored at 0 °C for 60 days. At the end of the storage, compared with the control samples, Lingwu long jujube treated with 1-MCP exhibited higher Vc bioaccessibility, total phenolic content and free radical scavenging capacity, which demonstrated that 1-MCP treatment could provide a better effect on maintaining the bioavailability of Lingwu long jujube.

scholarly journals Total phenolic content and antioxidant capacity of hydromethanolic extracts of in vitro and ex vitro plantlets of Drosera spp

2020 ◽  
Vol 5 (3) ◽  
pp. 17-32
Author(s):  
Ana Karina Carrera-Felipe ◽  
◽  
Sugey Vásquez-Hernández ◽  
María Teresa González-Arnao ◽  
Enrique Bonilla-Zavaleta ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Ex Vitro

scholarly journals EVALUATION OF DEHYDRATED MUKIA MADERASPATANA LEAVES AS A POTENT SOURCE OF DIETARY ANTIOXIDANTS

2019 ◽  
Vol 7 (6) ◽  
pp. 27-36
Author(s):  
D. Amirtham ◽  
J. Aswini Nivedida ◽  
K. Dhivya ◽  
S. Ganapathy ◽  
C. Indurani
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Green Leafy Vegetables ◽  
Total Antioxidant ◽  
Indigenous Plant

Green leafy vegetables are the most underexploited class of vegetables despite high nutritional value. The current study has been focused on the evaluation of anti-oxidant status of fresh and dehydrated under- utilized green leafy vegetable namely Mukia maderaspatana (L.) (Family: Cucurbitaceae), an indigenous plant; traditionally it is used as an ingredient of various cocktail preparations for the management of severe inflammatory disorders in Indian system of medicine. The total phenolic content (TPC), total flavonoid content (TFC) and total antioxidant activities were evaluated for the ethanolic extract of leaves to assess the in vitro antioxidant activities. The results showed that there exist a linear correlation between polyphenol content and antioxidant property. The ethanolic extract of dehydrated Mukia leaves showed the highest phenolic content (269.34 ± 0.78mg GAE/g), and total antioxidant activity (543±46 µmol Trolox/100 g). HPTLC analysis has revealed the presence of significant quantity of Quercitin (26.52%), an important flavonoid of tremendous antioxidant, anticancer and ant inflammatory properties in both the fresh and dehydrated leaves which might be the chief bioactive principle in Mukia.

scholarly journals Effect of Light-Emitting Diodes on the Mycelial Biomass Production and Antioxidant Activity of Ganoderma lucidum (W. Curt.: Fr.) P. Karst.

2021 ◽  
Vol 5 (1) ◽  
pp. 1-10
Author(s):  
Abegail Alcazar ◽  
◽  
Rich Milton Dulay ◽  
Sofronio Kalaw ◽  
Renato Reyes ◽  
...  
Keyword(s):  
Biomass Production ◽  
Ganoderma Lucidum ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Mycelial Biomass ◽  
Light Emitting ◽  
Colony Diameter ◽  
Red Led

Light is an important factor for the growth of many forms of life, including mushrooms. This paper highlights the effects of the different wavelengths (red, 650 nm; blue, 450 nm; green, 525 nm) of light emitting diodes (LEDs) on the growth, biomass production and antioxidant properties of Ganoderma lucidum. G. lucidum is a white-rot, wood-degrading fungus in the Basiodiomycota that typically grows on logs. Mycelia were grown on coconut water agar (CWA) solid media for analysis of mycelial colony diameter as well as coconut water (CW) submerged culture for analysis of mycelial biomass weight. Both set-ups were incubated in variously-colored LED chambers. The DPPH radical scavenging activity and total phenolic content of the harvested mycelia were also determined. After three days of incubation, G. lucidum mycelia under red LED recorded the highest mean mycelial colony diameter of 72.50 mm. In terms of mycelia biomass production, G. lucidum mycelia exposed in red LED and dark produced the heaviest weight. On the other hand, mycelia grown under green LED had the highest radical scavenging activity of 66.49% while those harvested from red LED showed the highest total phenolic content of 81.29 mg GAE / g of sample. Our results demonstrate that LED color influences the mycelial growth, biomass production and antioxidant activities of G. lucidum.

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