scholarly journals Virulence of Aerial Conidia of Beauveria bassiana Produced under LED Light to Ctenocephalides felis (Cat Flea)

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Sarayut Pittarate ◽  
Malee Thungrabeab ◽  
Supamit Mekchay ◽  
Patcharin Krutmuang
Keyword(s):  
Beauveria Bassiana ◽  
Adult Mortality ◽  
Ctenocephalides Felis ◽  
Fluorescent Light ◽  
Malt Extract ◽  
Led Light ◽  
Red Led ◽  
Aerial Conidia ◽  
Different Color

Ctenocephalides felis is an ectoparasitic flea species commonly found on dogs and cats. The current study verified the in vitro virulence of conidia of the entomopathogenic fungus Beauveria bassiana produced under different color LED light (red, blue, purple, green, yellow, and white) to adults of C. felis. The fungal isolates were cultivated on malt extract agar (MEA). Bioassay treatments used aerial conidia in test tubes. Adult fleas were obtained from a house cat in Chiang Mai province, Thailand. The experiments were composed of one control and eleven treatment groups. All of the treatments with B. bassiana conidia caused adult mortality after an exposure of 12 h. Among the conditions used in this study, B. bassiana cultured under red LED and fluorescent light were the most effective in causing mortality (100 %) in adult fleas after 36 h. The experimental results indicate that these aerial conidia of B. bassiana have promising potential for use in control of C. felis adult stages.

scholarly journals An RNA-Seq Analysis of Grape Plantlets Grown in vitro Reveals Different Responses to Blue, Green, Red LED Light, and White Fluorescent Light

2017 ◽  
Vol 8 ◽  
Author(s):  
Chun-Xia Li ◽  
Zhi-Gang Xu ◽  
Rui-Qi Dong ◽  
Sheng-Xin Chang ◽  
Lian-Zhen Wang ◽  
...  
Keyword(s):  
Fluorescent Light ◽  
Rna Seq ◽  
Led Light ◽  
Red Led

scholarly journals Effect of thidiazuron and monochromatic light on shoot development in vitro culture of Polygonum cuspidatum Sieb et Zucc

2020 ◽  
Vol 4 (2) ◽  
pp. First
Author(s):  
Doan Phan Phuong Thao ◽  
Tuan Anh Le ◽  
Phan Ngo Hoang
Keyword(s):  
Blue Light ◽  
Phenolic Content ◽  
Monochromatic Light ◽  
Total Phenolic ◽  
Fluorescent Light ◽  
Polygonum Cuspidatum ◽  
Blue Led ◽  
Red Led ◽  
Number Of Shoots

Polygonum cuspidatum Sieb. et Zucc, a medicinal plant, contains many phenolic compounds such as resveratrol. It has antioxidant, antibacterial, anti-cancer, HIV, and neuron protective properties. In this study, stem segments having an auxiliary bud were cultured in vitro on MS medium supplemented with TDZ at 0.1 mg/L for obtaining the highest number of shoots (8–9 shoots/cluster after eight weeks). The bud shoots were originated from the differentiation in the periphery of the lateral meristem. After eight weeks, the shoots cultured under the monochromatic light showed that the plant height, fresh and dry weight was higher than those grown under the fluorescent light at the same intensity of 50 µmol photon/m2/sec. Blue LED reduced the number of shoots/cluster, and respiration intensity of the inoculum compared to the red LED or fluorescent light conditions. However, after eight weeks, the total sugar and phenolic content in leaves and stems, as well as the resveratrol content of shoots under blue light, were always higher than under red LED or fluorescent light. Moreover, when samples were exposed under the one-week blue light condition at a double intensity (100 µmol photon/m2/sec), the shoot clusters growing under blue LED also had a remarkably high total phenolic content and significantly high resveratrol levels compared to the two other treatments. The roles of endogenous growth regulators in shoot clusters under different lighting conditions were also analyzed and discussed.

scholarly journals Evaluation of the effects of agricultural LED lighting on in vitro propagation of Cordyceps militaris (Link.) Fries

2020 ◽  
Vol 17 (3) ◽  
pp. 473-481
Author(s):  
Do Hong Gam ◽  
Duong Huong Huynh ◽  
Phan Thi Lan Anh ◽  
Nguyen Hoang Duong ◽  
Do Thi Kim Hoa
Keyword(s):  
Growth And Development ◽  
In Vitro Propagation ◽  
Positive Impact ◽  
The Other ◽  
Fluorescent Light ◽  
Led Lighting ◽  
Fresh Biomass ◽  
Red Led ◽  
Led Lights

In this study, the effects of various agricultural LED lights (LED NN), including single red LED (R), single blue LED (B), and four combinations of blue, red, and warm white (W) LED (BR, BRW1, BRW2, BRW3) on the growth and development of C. militaris (Link.) Fries were evaluated in vitro. After 7 days, samples subjected to LED NN showed shorter sporocarp sprouting time and higher sprouting ratio than the control, which was subjected to T5 fluorescent light. After 2 months, LED lights with high red ratio, such as single red LED and LED BR, had suppressing effect on the growth and development of C. militaris (Link.) Fries. On the other hand, combinations of red, blue, and warm white such as LED BRW1, LED BRW2, and LED BRW3 had the positive impact on the growth and development of this fungus. Notably, samples subjected to LED BRW2 reached 5.79 cm in height, fresh biomass of 3.67 g/20 samples. Cordycepin and Adenosine levels were 64.2 and 6.37 mg/100 g fresh mass, respectively. All of studied  indicators were the higher compared to those of the control and other LED lighting schemes. Therefore, it can be conlcuded that LED lighting combination with BRW2 ratio of 1:5:1 and luminous intensity of 45±2 µmol.m-2.s-1 (511,59 Lux) was suitable for the growth and development of C. militaris (Link.) Friesand a potential replacement of fluorescent light for C. militaris (Link.) Friesin vitro propagation.

Compatibility of Zineb, Dimethoate and Beauveria bassiana (Balsamo) Vuillemin Against Tarnished Plant Bug (Hemiptera: Miridae)

2003 ◽  
Vol 38 (3) ◽  
pp. 359-367 ◽  
Author(s):  
Mathias Kouassi ◽  
Daniel Coderre ◽  
Silvia I. Todorova
Keyword(s):  
Beauveria Bassiana ◽  
Adult Mortality ◽  
Lygus Lineolaris ◽  
Phenological Stage ◽  
Highly Pathogenic ◽  
Tarnished Plant Bug ◽  
Lactuca Sativa L ◽  
Dimethyl Dithiocarbamate ◽  
Laboratory Bioassays

Experiments were conducted under laboratory and field conditions to assess the effect of the fungicide Zineb (zinc dimethyl-dithiocarbamate) and the insecticide Cygon (dimethoate) on the pathogenicity of the isolate MK 2001 of Beauveria bassiana (Balsamo) Vuillemin, a potential microbial agent for biological control of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois). The isolate was highly pathogenic to adults yielding an LC50 of 7.2 × 103 conidia/mL at 6 d post treatment and an LT50 of 3.07 d at a concentration of 1 × 108 conidia/mL. Zineb inhibited the in vitro growth of the fungus in Sabouraud's dextrose broth and on Sabouraud's dextrose agar. It had no insecticidal activity against adult L. lineolaris in laboratory bioassays. A field test with caged L. lineolaris adults on lettuce Lactuca sativa L. var. longifolia, at a phenological stage of 50 leaves further demonstrated that Zineb and Dimethoate had no effect on B. bassiana against L. lineolaris. Adult mortality 10 d after application did not differ significantly in treatments with B. bassiana alone from treatments with B. bassiana + Zineb, dimethoate alone, B. bassiana + dimethoate, B. bassiana + Zineb, and B. bassiana + Zineb + dimethoate. No larva was observed in cages treated with B. bassiana alone or in combination with Zineb or dimethoate. On the contrary, 11 larvae/plant were recorded in the untreated cages and 5 larvae/plant in the dimethoate-treated cages.

In vitro metabolism study of normal and tumor cells when exposed to red LED light

2016 ◽  
Author(s):  
Olga V. Stolbovskaya ◽  
Radik M. Khairullin ◽  
Yuri V. Saenko ◽  
Ekaterina S. Krasnikova ◽  
Aleksandr V. Krasnikov ◽  
...  
Keyword(s):  
Tumor Cells ◽  
In Vitro Metabolism ◽  
Led Light ◽  
Red Led ◽  
Metabolism Study

scholarly journals In vitro seed germination and seedling growth of Calanthe discolor Lindl

2015 ◽  
Vol 71 (1) ◽  
pp. 109-119 ◽  
Author(s):  
Kee Hwa Bae ◽  
Kyoung Hee Oh ◽  
Soo-Young Kim
Keyword(s):  
Seedling Growth ◽  
Culture Media ◽  
Suitable Condition ◽  
Fluorescent Light ◽  
Immature Seeds ◽  
Red Led ◽  
Led Lights ◽  
Protocorm Formation ◽  
Germination And Growth

Abstract We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LED lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LED light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.

FLUORESCENT LIGHT (FL), RED LED AND BLUE LED SPECTRUMS EFFECTS ON IN VITRO SHOOTS MULTIPLICATION

2016 ◽  
Vol 78 (6-6) ◽  
Author(s):  
Nurul Syahirah Azmi ◽  
Robiah Ahmad ◽  
Rusli Ibrahim
Keyword(s):  
Large Scale ◽  
Ornamental Plants ◽  
Fruit Production ◽  
Fluorescent Light ◽  
Alternative Source ◽  
Light Emitting ◽  
Blue Led ◽  
In Vitro Shoots ◽  
Red Led

Tissue culture in ornamental plants is one of the relevant factors that beat production of vegetables and fruit production worldwide. It has been recognized as an effective tool to enhance large scale of plant multiplication. However, the conventional lighting system may contain unnecessary wavelength that are low quality to promote growth. In this study, experiment was conducted by using Light Emitting Diodes (LED) as an alternative source of lighting. Red and blue LEDs along with fluorescent light (FL) were applied to determine the best source of light in multiplication of rose. Under the same media regimes which are MS media basal and BAP shoot hormone, blue LED had shown more shoots and leaves. 

scholarly journals The influence of different wavelengths of LED light on the production of glucosinolates and phenolic compounds and the antioxidant potential in in vitro cultures of Nasturtium officinale (watercress)

2021 ◽  
Author(s):  
Marta Klimek-Szczykutowicz ◽  
Barbara Prokopiuk ◽  
Kinga Dziurka ◽  
Bożena Pawłowska ◽  
Halina Ekiert ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Blue Light ◽  
Photosynthetic Pigments ◽  
Soluble Sugars ◽  
Antioxidant Potential ◽  
Fluorescent Light ◽  
Biomass Growth ◽  
Led Light ◽  
Nasturtium Officinale

AbstractCultures of Nasturtium officinale were cultivated in vitro under illumination with different wavelengths of light-emitting diode (LED) light (white LED light—WLED, blue light—B, red light—R, 70% red and 30% blue light—RB, 50% green, 35% red and 15% blue light—RBG, 50% yellow, 35% red and 15% blue light—RBY, 50% far red, 35% red and 15% blue light—RBfR, 50% UV, 35% red and 15% blue light—RBUV, darkness—D), and under white fluorescent light (WF)—control conditions. The study investigated the influence of the applied lighting conditions on biomass growth and the production of glucosinolates, phenolic compounds, as well as photosynthetic pigments, and soluble sugars. The study showed a significant beneficial effect of the RBG light on biomass growth (Gi = 11.81 after 20 days) and the production of glucosinolates. The total glucosinolate content under these conditions increased 5.8 and 1.4 times in comparison with the WF light and D condition, respectively, reaching 237.92 mg 100 g−1 DW. The production of phenolic compounds, sugars, and photosynthetic pigments was comparable to the production under the control conditions. The antioxidant potential of extracts from the cultivated biomass was assessed by the CUPRAC, DPPH, and FRAP assays. Extracts obtained from the biomass of cultures grown under the RBG light had an antioxidant potential similar to that of the control cultures. This is the first report providing evidence of the stimulating effect of light quality on the biomass yield and production of glucosinolates by N. officinale microshoot cultures in vitro.

scholarly journals Uptake of the fluorescent probe FM4-64 by hyphae and haemolymph-derived in vivo hyphal bodies of the entomopathogenic fungus Beauveria bassiana

Microbiology ◽  
2009 ◽  
Vol 155 (9) ◽  
pp. 3110-3120 ◽  
Author(s):  
Michael W. Lewis ◽  
Ines V. Robalino ◽  
Nemat O. Keyhani
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Developmental Stages ◽  
Biological Control Agent ◽  
Fungal Mycelium ◽  
Dependent Manner ◽  
Aerial Conidia ◽  
Hyphal Bodies

The entomopathogenic fungus Beauveria bassiana is under intensive study as a pest biological control agent. B. bassiana produces several distinct single-cell types that include aerial conidia, in vitro blastospores and submerged conidia. Under appropriate nutrient conditions these cells can elaborate germ tubes that form hyphae, which in turn lead to the formation of a fungal mycelium. In addition, B. bassiana displays a dimorphic transition, producing in vivo specific yeast-like hyphal bodies during growth in the arthropod haemolymph. The amphiphilic styryl dye FM4-64 was used to investigate internalization and morphological features of in vitro and in vivo insect haemolymph-derived B. bassiana cells. In vitro blastospores and submerged conidia displayed a punctate pattern of internal labelling, whereas aerial conidia failed to internalize the dye under the conditions tested. FM4-64 was also taken up into both apical and subapical compartments of living hyphae in a time-dependent manner, with clearly observable vesicle labelling. Internalization, where occurring, was reversibly disrupted by lowering the temperature of the assay or by treatment with azide/fluoride and latrunculin A. Treatment with cytochalasin D and monensin also caused abnormal vesicle trafficking, although some staining of vesicles was noted. Fungal cells derived from infected Heliothis virescens haemolymph (in vivo cells) actively internalized FM4-64. The in vivo blastospores or hyphal bodies displayed bright membrane and internal vesicle staining, although diffuse staining of internal structures was also visible. These results suggest active uptake by different developmental stages of B. bassiana, including haemolymph-derived cells that can evade the insect immune system.

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