scholarly journals In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Puneet Singh ◽  
Yogesh Sharma ◽  
Ashutosh Sharma
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Local Market ◽  
Plant Materials ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory ◽  
Convolvulus Pluricaulis

Aim: In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy. Material & Methods- The whole plant parts of Convolvulus pluricaulis Choisy were purchased from the local market. Whole plant materials were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of whole plant material was extracted using 95 % methanol by soxhlet apparatus for 72 h. Qualitative chemical tests of methanolic extracts were subjected to various chemical tests to detect various phytoconstituents. Solvent systems ethyl acetate: methanol: water (77:13:10) were found to be most satisfactory solvent system. After development of plates, they were air-dried and number of spots, color and Rf values were recorded. The % heamolysis was calculated by assuming the heamolysis produced by the control group as 100 %. Results: The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds. At a concentration of 500 µg/ml, the extract produced 71.59% protection of RBC haemolysis as compared with 72.73% produced by prednisolone.  The methanolic extract of selected plant showed 39.70% inhibition. The Diclofenac sodium showed 55.88 % inhibition against denaturation of protein. Conclusion: In conclusion, it can be stated that the methanolic extract has beneficial effects in long lasting in membrane stabilizing method, inhibition of protein denaturation method and proteinase model. Keywords: In-Vitro, Anti-inflammatory Activity, Methanolic Extract, Convolvulus pluricaulis Choisy, Protein Denaturation Method

Phytochemical and pharmacological evaluation of Tragia cannabina for anti-inflammatory activity

2012 ◽  
Vol 1 (8) ◽  
pp. 213 ◽  
Author(s):  
Raju Venkategowda Hosahally ◽  
Ganapaty Seru ◽  
Prasanna Shankarrao Sutar ◽  
Vijaya Gopalachar Joshi ◽  
Kishori Prasanna Sutar ◽  
...  
Keyword(s):  
Body Weight ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Inflammatory Activity ◽  
Reference Standard ◽  
Paw Edema ◽  
Pharmacological Evaluation ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory

<p>The present study investigates the anti-inflammatory activity of methanolic and chloroform extracts of<strong><em> </em></strong><em>Tragia cannabina</em>. The whole plant of <em>Tragia cannabina</em> was extracted with methanol and chloroform by using soxhlet apparatus. The effect of both extracts of <em>Tragia cannabina</em> was studied on carrageenan induced paw edema. The methanolic extract decreased the edema induced in hind paw. The percentage inhibition of paw edema was maximum with methanolic and chloroform extracts of <em>Tragia cannabina</em> at 300mg/kg body weight and has showed significant anti-inflammatory activity. It has been concluded that both the methanolic and chloroform extracts of <em>Tragia cannabina</em> showed significant anti-inflammatory activity comparable to that of reference standard Ibuprofen.</p><p>DOI: <a href="http://dx.doi.org/10.3329/icpj.v1i8.10857">http://dx.doi.org/10.3329/icpj.v1i8.10857</a></p> <p>International Current Pharmaceutical Journal 2012, 1(8): 213-216</p><p> </p>

IN VITRO ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT AND DIFFERENT FRACTIONS OF CENTELLA ASIATICA LEAVES BY PROTEIN DENATURATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (06) ◽  
pp. 86-89
Author(s):  
S Sharma ◽  
◽  
R. Trivedi ◽  
N. K. Choudhary
Keyword(s):  
Petroleum Ether ◽  
Skin Diseases ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Centella Asiatica ◽  
Inflammatory Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Inflammation might be a complex organic reaction to a hazardous stimulant such as pathogens, or injured tissues and mainly causes itching, swelling, skin redness, warm and slight pain. Herbal drugs are widespread in India for their effectiveness, easy availability at low cost and provide low toxicity as compared to modern drugs. Centella asiatica is one of the oldest Ayurvedic medicinal plants, used in treatment of various skin diseases. The aim of our present research was to evaluate the in vitro anti-inflammatory activity of methanolic extract and different fractions of C. asiatica leaves. In protein denaturation method, the percentage inhibition for methanolic extract was observed to be 40.22%. The petroleum ether and n-butanol fraction of methanolic extract of C. asiatica were observed to exhibits 54.12 and 44.42% inhibition, respectively. Diclofenac sodium was used as a standard drug. In comparison with other fractions petroleum ether and n-butanol fractions showed best activity. The preliminary phytochemical studies of n-butanol fractions and n-butanol fractions showed the presence of terpenoids, flavonoids etc., which are used in the treatment of inflammation. Thus, we can call the latter as intense anti-inflammatory agent.

scholarly journals Assessment of In vitro Anti-inflammatory Activity of Ginger and Diclofenac sodium combination

2020 ◽  
pp. 442-447
Author(s):  
Mousmi D. Thakur ◽  
Navin R. Sheth ◽  
Mihir K. Raval
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Research Work ◽  
Pharmacological Action ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Ginger Extract ◽  
Anti Inflammatory

The present research work aimed at evaluating the anti-inflammatory activity of Zingiber officinalis with Diclofenac sodium by HRBC membrane stabilization & Protein denaturation. The precluding of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization at different concentrations was performed for methanolic, hydro-methanolic ginger extract and diclofenac sodium. At a dose of 50µg/ml the maximum membrane stabilization 86.34% was found for Ginger extract(test) and at a dose of 500 mcg/ml membrane stabilization was found 91.16% for diclofenac sodium(standard) and the membrane stabilization for combination (ginger with diclofenac sodium) at a dose of 50µg/ml was recorded 86.43%, as the concentration increase(1000 mcg/ml) for combination(ginger with diclofenac sodium) the percentage protection was decreased. In vitro protein denaturation was performed by using egg albumin method. Maximum inhibition was observed in case of methanolic extract of ginger at concentration 1000mcg/ml and it was 78.83±5.17 and in hydro methanolic extract for Diclofenac sodium at concentration 1000mcg/ml and it was 63.37±2.78.Minimum inhibition observed in combination of methanolic extract of ginger and diclofenac sodium at concentration 1000mcg/ml and it was 25.27±1.76 and in combination of hydro-methanolic extract of ginger and Diclofenac sodium at concentration 1000mcg/ml and it was 28.23±3.14. The results of this study divulge that low dose combination of ginger and diclofenac sodium has higher anti-inflammatory activity than diclofenac sodium and ginger alone. With this initial study, research work could be extended further; therefore, the particular pharmacological action for the combination of ginger with diclofenac sodium could be discovered.

scholarly journals EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS

2017 ◽  
pp. 198-200
Author(s):  
Biresh Kumar Sarkar ◽  
Ravi Kumar ◽  
Reeta . ◽  
S. C. Verma ◽  
Shashi Pal ◽  
...  
Keyword(s):  
Chromatographic Analysis ◽  
Protein Denaturation ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Alcoholic Extract ◽  
Plant Materials ◽  
Anti Inflammatory ◽  
Phyllanthus Fraternus ◽  
Analysis And Study

Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids. 

scholarly journals Phytochemical and pharmacological evaluation of Tragia cannabina for anti-inflammatory activity

2012 ◽  
Vol 1 (8) ◽  
pp. 213-216 ◽  
Author(s):  
Raju Venkategowda Hosahally ◽  
Ganapaty Seru ◽  
Prasanna Shankarrao Sutar ◽  
Vijaya Gopalachar Joshi ◽  
Kishori Prasanna Sutar ◽  
...  
Keyword(s):  
Body Weight ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Inflammatory Activity ◽  
Reference Standard ◽  
Paw Edema ◽  
Pharmacological Evaluation ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory

The present study investigates the anti-inflammatory activity of methanolic and chloroform extracts of Tragia cannabina. The whole plant of Tragia cannabina was extracted with methanol and chloroform by using soxhlet apparatus. The effect of both extracts of Tragia cannabina was studied on carrageenan induced paw edema. The methanolic extract decreased the edema induced in hind paw. The percentage inhibition of paw edema was maximum with methanolic and chloroform extracts of Tragia cannabina at 300mg/kg body weight and has showed significant anti-inflammatory activity. It has been concluded that both the methanolic and chloroform extracts of Tragia cannabina showed significant anti-inflammatory activity comparable to that of reference standard Ibuprofen.DOI: http://dx.doi.org/10.3329/icpj.v1i8.11253 International Current Pharmaceutical Journal 2012, 1(8): 213-216 

scholarly journals Preliminary phytochemical screenings and Pharmacological activities of Blumea lacera (Burn.f.) DC.

2015 ◽  
Vol 13 (1) ◽  
pp. 69-73
Author(s):  
Md Abul Khair ◽  
Mohammed Ibrahim ◽  
Qamrul Ahsan ◽  
Md Ruhul Kuddus ◽  
Ridwan Bin Rashid ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Crude Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Acetyl Salicylic Acid ◽  
Antipyretic Activity ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Blumea Lacera

The methanol extract of the whole plant of Blumea lacera (Burn.f.) DC. (BLME) has been subjected to preliminary screenings for phytoconstituents and antipyretic, analgesic and anti-inflammatory activities. Antipyretic activity was assessed by the yeast-induced hyperthermia in mice. The analgesic property was evaluated by formalin-induced writhing test. Acetyl salicylic acid (ASA) was used as standard for in-vitro anti-inflammatory activity test. In yeast-induced pyrexia, the crude extract demonstrated a significant (p=0.05) reduction in body temperature of mice after elevation by the administration of yeast. These effects were pronounced at the 2nd and 3rd h of post-treatment with the extract. BLME exhibited a dose-dependent analgesic activity with 39.13% and 56.52% protection at 200-and 400-mg/kg, b.w., respectively as compared to 76.09% revealed by the standard diclofenac sodium. In the anti-inflammatory test, the crude extract at 400 ?g/ml displayed 62.40% inhibition of protein denaturation whereas standard acetyl salicylic acid exhibited 76.74% inhibition. Results of the preliminary phytochemical screenings demonstrated the presence of alkaloids, flavonoids and triterpenoids in the extract. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21863 Dhaka Univ. J. Pharm. Sci. 13(1): 69-73, 2014 (June)

scholarly journals Synthesis, Characterization and Evaluation of Anti-inflammatory Activity of Novel Indoline Derivatives

2016 ◽  
Vol 12 (12) ◽  
pp. 4557-4563
Author(s):  
Dhinesh kumar Manoharan
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Potent Inhibition ◽  
Ft Ir ◽  
Anti Inflammatory

Series of indoline derivatives were synthesized using N-(4-aminophenyl) indoline-1-carbothiamide as a precursor. The structures of synthesized compounds were confirmed by   FT-IR, 1H-NMR, 13C-NMR and LC-MS. The in vitro anti-inflammatory activity of synthesized indoline derivatives were examined by standard anti-denaturation assay. The compounds 4a (IC50 = 62.2 µg/ml) and 4b (IC50 = 60.7 µg/ml) showed potent inhibition on protein denaturation. The compounds 5a (IC50 = 97.8 µg/ml) exhibits moderate inhibition on protein denaturation

scholarly journals Anti-inflammatory activity of Punica granatum extract on oral microbes - In vitro

2019 ◽  
Vol 10 (2) ◽  
pp. 1019-1022
Author(s):  
Westeros Dominic Pereira ◽  
Geetha RV ◽  
Lakshmi Thangavelu
Keyword(s):  
Protein Denaturation ◽  
Periodontal Diseases ◽  
Punica Granatum ◽  
Inflammatory Activity ◽  
Oral Diseases ◽  
Major Health Problem ◽  
Maximum Inhibition ◽  
Anti Inflammatory ◽  
Oral Microbes

To study the anti-inflammatory effect of Punica granatum extract against the oral microbes. Oral diseases continue to be a major health problem worldwide. Dental caries and periodontal diseases are among the most important global oral health problems, although conditions such as oral and pharyngeal cancers and oral tissue lesions are also significant health concerns. Pomegranate extracts have been used for centuries in traditional medicine to confer health benefits in a number of inflammatory diseases, microbial infections and cancer. The anti-inflammatory activity of pomegranate extract was evaluated by protein denaturation assay, and the results were read spectrophotometrically. Denaturation of proteins is a great‐ documented cause of inflammation. As a part of the investigation on the mechanism of the anti-inflammatory activity, the ability to extract to inhibit protein denaturation was studied. It was effective in inhibiting heat induced albumin denaturation at different concentrations as shown in Table 1. Maximum inhibition, 70.12±1.12% was observed at500µg/ml. IC50 value was found to be 105.35±1.99µg/ml. Aspirin, a standard anti-inflammatory drug showed the maximum inhibition, 77.12±1.42% at the concentration of 200µg/ml. Hence it can be concluded that pomegranate extract has anti-inflammatory property and also can be used in products such as toothpaste and mouth wash etc.

scholarly journals In vitro Evaluation of Anti-Inflammatory Activity of Symplocos racemosa Using Protein Denaturation Assay

2021 ◽  
pp. 715-720
Author(s):  
K. Janani ◽  
R. V. Geetha ◽  
S. Rajeshkumar
Keyword(s):  
Plant Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Whatman Filter Paper ◽  
Traditional Drug ◽  
Herbal Plants ◽  
Anti Inflammatory ◽  
Herbal Formulations ◽  
Symplocos Racemosa

Introduction: Recently there is considerable awareness and interest in the field of herbal medicine due to its natural origin and lesser side effects compared to Allopathy. Selected herbal plants like Symplocos racemosa, commonly known as lodhra, are found mainly in plains and lower hills of Bengal. The word ‘Lodhra’ means ‘Propitious’. Symplocos racemosa is an important Indian traditional drug used in many Ayurvedic and herbal formulations for treatment of liver as well as uterine disorders and leucorrhea. Ethnobotanical Literature indicates use of Symplocos racemosa in treatment of eye disease, skin disease, ear disorders, liver and bowel complaints, tumours,uterine disorders, spongy and bleeding gums, asthma, fever, snakebite, gonorrhoea and arthritis. Aim: To analyse the anti-inflammatory activity of Symplocos racemosa using protein denaturation assay. Materials and Methods: 2 g of Lodhra bark powder is mixed with 100 ml distilled water & boiled for 20 min at 50°C. The extract is filtered using whatman filter paper & concentrated to 10 ml.1 ml each of Bovine serum albumin is added to various fixations of plant extract (10μL - 50 μL) and the anti - inflammatory activity was evaluated by analysing the percentage inhibition. Results: From this study, it is evident that Lodhra has significant anti-inflammatory activity. At 50μℓ concentration, the plant extract shower higher anti- inflammatory activity of 76%. Conclusion: Symplocos racemosa extract has proved to exhibit effective anti- inflammatory activity. Further studies have to be carried to analyse the other properties of this herb, which can be incorporated successfully in the pharmaceutical industry.

scholarly journals Demonstration of Phytochemicals and In-vitro Antioxidant and Anti-inflammatory activity by methanolic extract of Elettaria cardamomum

2020 ◽  
Vol 3 (3) ◽  
pp. 01-04
Author(s):  
Md. Shahidul Islam
Keyword(s):  
Methanolic Extract ◽  
Research Work ◽  
Inflammatory Activity ◽  
Pharmacological Activities ◽  
Elettaria Cardamomum ◽  
Ic50 Values ◽  
Phytochemical Profile ◽  
Anti Inflammatory ◽  
Dpph Scavenging

The existing research study attempts to untie novel avenues for development of the medicinal exercises of Elettaria cardamomum, fashionable known as the “Queen of Spices” and locally recognized as “elaichi”. Its seeds are utilized as abortifacient, acrid, alexiteric, aromatic, sweet, cardiac tonic, cooling, carminative, digestive, diuretic, expectorant, stimulant and also tonic beneficial in the asthma, haemorrhoids, bronchitis, strangury, renal in addition to vesical calculi, anorexia, halitosis, gastropathy dyspepsia as well as burning sensation. The prime goal of this research work is to evaluate antioxidant as well as anti-inflammatory properties of the traditional Bangladeshi medicinal extracts in addition to examine these activities. The aim in present work was to screen the phytochemical profile as well as pharmacological activities of the methanolic extract of this plant’s leaves. To explore pharmacological actions DPPH scavenging test and the HRBC membrane stabilization techniques were done for the antioxidant and also anti-inflammatory test respectively. The pharmacological works revealed that plant extracts might have noteworthy antioxidant effect which is possibly mediated by the inhibition of DPPH free radical which is accountable for oxidation. The IC50 values by the DPPH scavenging test observed for the standard and the leaves were 106.38µg/ml & 594.47µg/ml respectively. There is also moderate anti-inflammatory activity. The IC50 values for anti-inflammatory activity by standard & leaves were 35.04µg/ml and 944.0 µg/ml respectively.

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