scholarly journals Protective Effect of Catechin Hydrate on Spinal Cord Ischemia-reperfusion Injury in Rats

2021 ◽  
Vol 7 (4) ◽  
pp. 193-201
Author(s):  
Naser Hajizadeh ◽  
◽  
Gholam Hossein Farjah ◽  
Mojtaba Karimipour ◽  
Bagher Pourheidar ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Plasma Level ◽  
Ischemia Reperfusion ◽  
Spinal Cord Ischemia ◽  
Male Rats ◽  
Motor Deficit ◽  
High Dose ◽  
Catechin Hydrate ◽  
Sham Surgery ◽  
The Mean

Background: Aortic artery stenosis leads to Ischemia-Reperfusion (I-R) injury, which can cause certain clinical expressions, such as paraplegia. Objectives: To appraise the effect of Catechin Hydrate (CH) against spinal cord I-R injury. Materials & Methods: A total of 35 male rats (250-300 g) were divided randomly into five groups: intact, sham surgery, dimethyl sulfoxide (I-R+DMSO), low-dose CH (I-R+10 mg/kg CH), and high-dose CH (I-R+20 mg/kg CH). Abdominal aorta clamping was done for 60 min. Seventy-two hours after I-R, animals were evaluated for neurologic function, biochemical analysis, and histology. The data analysis was conducted by SPSS v. 16 using ANOVA and Kruskal-Wallis and Mann-Whitney U tests. Results: The mean Motor Deficit Index (MDI) score and white matter damage in the CH (20 mg/kg) group were lower than in the DMSO group (P=0.032). The mean plasma levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the CH groups were lower than that of the DMSO group (P<0.05). The plasma level of Total Antioxidant Capacity (TAC) in the CH (20 mg/kg) group was higher than in the DMSO group (P=0.032). In addition, the plasma level of catalase in the CH (20 mg/kg) group was higher than in the DMSO and CH (10 mg/kg) groups (P<0.001). The average number of normal motor neurons in the experimental groups was lower than in the sham surgery group (P<0.001).

scholarly journals Antioxidative and anti-inflammatory effects of Cichorium intybus L. seed extract in ischemia/reperfusion injury model of rat spinal cord

2018 ◽  
Vol 4 (4) ◽  
Keyword(s):  
Spinal Cord ◽  
Treatment Group ◽  
Aqueous Extract ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Spinal Cord Ischemia ◽  
Cichorium Intybus ◽  
Inflammatory Factors ◽  
The Mean ◽  
Anti Inflammatory

Objectives: The antioxidant and anti-inflammatory effects of aqueous extract of chicory seed (CSE, Cichorium intybus L. seed) following spinal cord ischemia/reperfusion (SCI/R) injury in rat model were evaluated. Methods: In this study 36 male Wistar rats were randomly divided to six groups: control (Co), sham (Sh), CSE, SCI/R, CSE+SCI/R (7 days pretreatment with CSE group+inducing I/R injury), SCI/R +CSE (induced I/R injury group+3 days treatment with CSE). SCI/R injury was induced by creating a longitudinal incision on the midline of abdominal region and clamping the aorta just below renal artery for 30 minutes. After 3 days, SC was removed and used for evaluation of antioxidant enzymes (including Superoxide dismutase [SOD] and catalase [CAT]), oxidative stress markers (malondialdehyde [MDA]), inflammatory factors (IL1β, IL18 & TNFα) and histopathological changes. Before sacrificing the animals, the motional score were assessed. Results: Our results demonstrated that, in the SCI/R group, the mean levels of SOD, and CAT were significantly decreased (P<0.05), while the mean level of MDA was significantly increased (P<0.05) in comparison to Co and Sh groups. Also, the mean levels of SOD and CAT in the treatment group were higher than the SCI/R group (P<0.05), while, the mean MDA content in the treatment group was significantly less than the SCI/R group (P <0.05). In addition, comparison between SCI/R and treatment groups demonstrated a significant decrease in tissue damage in the treatment group. Conclusions: Our study demonstrated that, the neuroprotective effects of aqueous extract of Cichorium intybus L. seed on SCI/R injury in rat by anti-oxidative and anti-inflammatory activities. Additionally, comparing the treatment and pretreatment groups shows that the pretreatment usage of the extract is more effective than treatment group.

scholarly journals Dexmedetomidine Attenuates Blood-Spinal Cord Barrier Disruption Induced by Spinal Cord Ischemia Reperfusion Injury in Rats

2015 ◽  
Vol 36 (1) ◽  
pp. 373-383 ◽  
Author(s):  
Bo Fang ◽  
Xiao-Qian Li ◽  
Bo Bi ◽  
Wen-Fei Tan ◽  
Gang Liu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Motor Neurons ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Spinal Cord Ischemia ◽  
Beneficial Effects ◽  
Polymerase Chain ◽  
Underlying Mechanisms ◽  
Blood Spinal Cord Barrier ◽  
Metalloproteinase 9

Background/Aims: Dexmedetomidine has beneficial effects on ischemia reperfusion (I/R) injury to the spinal cord, but the underlying mechanisms are not fully understood. This study investigated the effects and possible mechanisms of dexmedetomidine on blood-spinal cord barrier (BSCB) disruption induced by spinal cord I/R injury. Methods: Rats were intrathecally pretreated with dexmedetomidine or PBS control 30 minutes before undergoing 14-minute occlusion of aortic arch. Hind-limb motor function was assessed using Tarlov criteria, and motor neurons in the ventral gray matter were counted by histological examination. The permeability of the BSCB was examined using Evans blue (EB) as a vascular tracer. The spinal cord edema was evaluated using the wet-dry method. The expression and localization of matrix metalloproteinase-9 (MMP-9), Angiopoietin-1 (Ang1) and Tie2 were assessed by western blot, real-time polymerase chain reaction, and immunofluorescence. Results: Intrathecal preconditioning with dexmedetomidine minimized the neuromotor dysfunction and histopathological deficits, and attenuated EB extravasation after spinal cord I/R injury. In addition, dexmedetomidine preconditioning suppressed I/R-induced increase in MMP-9. Finally, Dexmedetomidine preconditioning enhanced the Ang1-Tie2 system activity after spinal cord I/R injury. Conclusions: Dexmedetomidine preconditioning stabilized the BSCB integrity against spinal cord I/R injury by inhibition of MMP-9, and enhancing the Ang1-Tie2 system.

Exosome miR-23a-3p From Osteoblast Alleviates Spinal Cord Ischemia/Reperfusion Injury by Down-regulating KLF3-activated CCNL2 Transcription

2021 ◽  
Author(s):  
Cheng Wu ◽  
Qinghua Zhu ◽  
Yi Yao ◽  
Zhaoyang Shi ◽  
Chaojie Jin ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Reperfusion Injury ◽  
Regulatory Mechanism ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Spinal Cord Ischemia ◽  
Neurological Dysfunction ◽  
Aneurysm Surgery ◽  
Aortic Aneurysm Surgery ◽  
Geo Database

Background: Spinal cord ischemia/reperfusion injury (SCIRI) is usually caused by spinal surgery or aortic aneurysm surgery and can eventually lead to paralysis or paraplegia and neurological dysfunction. Exosomes are considered as one of the most promising therapeutic strategies for SCIRI as they can pass the blood-spinal barrier. Previous studies have proved that exosomes secreted by osteocytes have a certain slowing effect on SCIRI. Aim: We aimed to explore the effect of osteoblast secreted exosomes on SCIRI. Methods: Firstly, neurons and osteoblasts were co-cultured under different conditions. GEO database was utilized to detect the expression of miR-23a-3p in osteoblast exosomes. SCIRI cells were treated with exosomes, and the detection was taken to prove whether miR-23a-3p could slow the progression of SCIRI. Downstream gene and the potential regulatory mechanism were explored through database and functional experiments. Results: MiR-23a-3p was highly expressed in exosomes and it slowed down the process of SCIRI. Downstream mRNA KLF3 could bind to miR-23a-3p and was highly expressed in IRI. Moreover, CCNL2 was regulated by KLF3 and was highly expressed in IRI. Rescue experiments verified that miR-23a-3p suppressed the transcription of CCNL2 by targeting KLF3. Conclusion: Exosome miR-23a-3p from osteoblast alleviates SCIRI by down-regulating KLF3-activated CCNL2 transcription.

Cilostazol Attenuates Spinal Cord Ischemia-Reperfusion Injury in Rabbits

2015 ◽  
Vol 29 (2) ◽  
pp. 351-359 ◽  
Author(s):  
Yunus Nazli ◽  
Necmettin Colak ◽  
Mehmet Namuslu ◽  
Husamettin Erdamar ◽  
Hacer Haltas ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Spinal Cord Ischemia
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