Saprophytic fluorescerous bacteria of the genus Pseudomonas of from natural mineral waters of some regions of Ukraine

2019 ◽  
Vol 92 (2) ◽  
pp. 50-53
Author(s):  
Svetlana NIKOLENKO ◽  
◽  
Elena NIKIPELOVA ◽  
T VANZHULA ◽  
Kristina KOYEVA
Keyword(s):  
Mineral Waters ◽  
Natural Mineral

Effect of Some Natural Mineral Waters in Nutrient Uptake by Caco-2 Cells

2010 ◽  
Vol 80 (2) ◽  
pp. 131-143 ◽  
Author(s):  
Pedro Gonçalves ◽  
João R. Araújo ◽  
Fátima Martel
Keyword(s):  
Nutrient Uptake ◽  
Mineral Waters ◽  
Distilled Water ◽  
Natural Mineral ◽  
Minute Exposure

We studied the effect of some mineral waters and some of their constituents on the apical uptake of 14C-butyrate (14C-BT) and 3H-O-methyl-D-glucose (3H-OMG) by Caco-2 cells. Uptake of 14C-BT increased after a 20-minute exposure to 1 % (v/v) distilled water, and, compared to distilled water, it was decreased by Pedras Salgadas® 1 % (v/v) and Melgaço® 5 % (v/v), and increased by Vidago® 5 % (v/v). Moreover, it increased after a 48-hour exposure to Vidago® or Melgaço® waters (5 % (v/v)). Also, uptake of 14C-BT was reduced after a 20-minute exposure to MgCl2, MgSO4, or CaCl2. Uptake of 3H-OMG was reduced after a 20-minute exposure to Melgaço® water [1 % (v/v)], when compared to distilled water. Also, a 48-hour exposure to Pedras Salgadas® or Melgaço® water (5 % (v/v)) increased and decreased uptake, respectively. Finally, uptake of 3H-OMG decreased after a 20-minute exposure to MgSO4 or NaF. In conclusion, uptake of 14C-BT and 3H-OMG by Caco-2 cells is differently modulated by distinct mineral waters.

scholarly journals Modified Exfoliated Carbon Nanoplatelets as Sorbents for Ammonium from Natural Mineral Waters

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3541
Author(s):  
Ion Ion ◽  
Daniela Bogdan ◽  
Monica Maria Mincu ◽  
Alina Catrinel Ion
Keyword(s):  
Natural Waters ◽  
Mineral Waters ◽  
Aqueous Samples ◽  
Sorption Mechanism ◽  
Characteristic Parameters ◽  
Sorption Characteristic ◽  
Natural Mineral ◽  
Maximum Sorption Capacity ◽  
Ammonium Removal ◽  
Maximum Sorption

In this manuscript an improved sorbent based on modified exfoliated carbon nanoplatelets, applied in the removal of ammonium from aqueous samples, is presented. This sorbent showed better efficiency in comparison with the previous one obtained in our group for ammonium removal, the values of the maximum sorption capacity being improved from 10 to 12.04 mg/g. In terms of kinetics and sorption characteristic parameters, their values were also improved. Based on these results, a sorption mechanism was proposed, taking into account ion-exchange and chemisorption processes at the surface of the oxidized exfoliated carbon nanoplatelets. Future applications for simultaneous removal of other positive charged contaminants from natural waters might be possible.

scholarly journals Reverse Transcription-PCR Analysis of Bottled and Natural Mineral Waters for the Presence of Noroviruses

2003 ◽  
Vol 69 (11) ◽  
pp. 6541-6549 ◽  
Author(s):  
Gilbert Thierry Lamothe ◽  
Thierry Putallaz ◽  
Han Joosten ◽  
Joey D. Marugg
Keyword(s):  
Reverse Transcription ◽  
Membrane Filtration ◽  
Limit Of Detection ◽  
Mineral Waters ◽  
Pcr Analysis ◽  
Rna Sequences ◽  
Natural Mineral ◽  
Reverse Transcription Pcr ◽  
Viral Particles ◽  
Nonspecific Amplification

ABSTRACT A seminested reverse transcription-PCR method coupled to membrane filtration was optimized to investigate the presence of norovirus (NV) RNA sequences in bottled and natural mineral waters. The recovery of viral particles by filtration varied between 28 and 45%, while the limit of detection of the overall method ranged from 6 to 95 viral particles. The assay was broadly reactive, as shown by the successful detection of 27 different viral strains representing 12 common genotypes of NVs. A total of 718 bottled and natural mineral water samples were investigated, including 640 samples of finished, spring, and line products (mostly 1 to 1.5 liters), collected from 36 different water brands of various types and from diverse geographic origins over a 2-year period. In addition, 78 samples of larger volume (10 and 400 to 500 liters) and environmental swabs were investigated. From the 1,436 analyses that were performed for the detection of NVs belonging to genogroups I and II, 34 samples (2.44%) were presumptively positive by seminested RT-PCR. However, confirmation by DNA sequence analysis revealed that all presumptive positive results were either due to nonspecific amplification or to cross-contamination. In conclusion, these results do not provide any evidence for the presence of NV genome sequences in bottled waters.

Natural Mineral Waters as a Medium for the Stock Daphnia magna Cultures for Bioassays

2021 ◽  
Vol 57 (2) ◽  
pp. 87-95
Author(s):  
I. M. Konovets ◽  
M. T. Goncharova ◽  
L. S. Kipnis ◽  
M. G. Mardarevich
Keyword(s):  
Daphnia Magna ◽  
Mineral Waters ◽  
Natural Mineral

Comparison of different concentration methods for the detection of hepatitis A virus and calicivirus from bottled natural mineral waters

2010 ◽  
Vol 165 (1) ◽  
pp. 57-63 ◽  
Author(s):  
S. Di Pasquale ◽  
M. Paniconi ◽  
B. Auricchio ◽  
L. Orefice ◽  
A.C. Schultz ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Mineral Waters ◽  
Natural Mineral ◽  
Concentration Methods

Assessment of the microbiological quality of natural mineral waters according to the manufacturing time of 20 L returnable packs in Brazil

2020 ◽  
Vol 367 (15) ◽  
Author(s):  
Isabelle da Silva Luz ◽  
Luiza Vasconcellos ◽  
Valéria de Mello Medeiros ◽  
Catia Aparecida Chaia Miranda ◽  
Carla de Oliveira Rosas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Microbiological Quality ◽  
Human Adenovirus ◽  
Mineral Waters ◽  
Microbiological Contamination ◽  
Natural Mineral ◽  
Norovirus Gii ◽  
Manufacturing Time ◽  
Sequence Types

ABSTRACT This study aimed to assess the microbiological quality of natural mineral waters commercialized in 20 L returnable packs in Brazil by investigating the presence of bacteria and viruses in packs with different manufacturing times (Tm). With this purpose, 99 water samples from 33 lots (n = 3/batch) of 15 brands, obtained from packs with three intervals of Tm, were analyzed. Total coliforms (16.2%), Pseudomonas aeruginosa (9.9%), sulphite-reducing Clostridium (5.0%) and Escherichia coli (2.0%) were detected but enterococci and norovirus GII not. Regarding brands, 11 (73.3%) presented unsatisfactory results for at least one of the lots analyzed. Pseudomonas aeruginosa analysis revealed six sequence types and strains were susceptible to all antibiotics tested and were able to produce biofilms. Human adenovirus (4) and norovirus GI (9) were also identified in nine samples randomly selected. Natural mineral waters commercialized in 20 L packs with Tm ≥ 2 years presented more microbiological contamination (P ≤ 0.012) than ones with a Tm of 0–1 year or a Tm of 1–2 years. These results suggest that the validity period of reusable 20 L packs should be reduced or that they can no longer be reused.

scholarly journals Natural mineral waters of the Chechen Republic: current usage and prospects for development

2020 ◽  
Vol 579 ◽  
pp. 012025
Author(s):  
Arun A Daukaev ◽  
H Sh Zaburaeva ◽  
L S Gatsaeva ◽  
Aslan A Daukaev ◽  
I V Sarkisyan ◽  
...  
Keyword(s):  
Mineral Waters ◽  
Natural Mineral ◽  
Current Usage

scholarly journals Diversity of Bacteria Growing in Natural Mineral Water after Bottling

2005 ◽  
Vol 71 (7) ◽  
pp. 3624-3632 ◽  
Author(s):  
Alexander Loy ◽  
Wolfgang Beisker ◽  
Harald Meier
Keyword(s):  
Bacterial Community ◽  
Bacterial Growth ◽  
Mineral Water ◽  
Bacterial Community Composition ◽  
Bacterial Flora ◽  
Significant Proportion ◽  
Mineral Waters ◽  
Rrna Gene ◽  
Natural Mineral ◽  
Natural Mineral Water

ABSTRACT Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bottling. Seven 16S rRNA gene libraries, comprising 108 clones in total, were constructed from water samples taken at various days after bottling and from two different bottle sizes. Sequence analyses identified 11 operational taxonomic units (OTUs), all but one affiliated with the betaproteobacterial order Burkholderiales (6 OTUs) or the class Alphaproteobacteria (4 OTUs). Fluorescence in situ hybridization (FISH) was applied in combination with DAPI (4′,6′-diamidino-2-phenylindole) staining, viability staining, and microscopic counting to quantitatively monitor changes in bacterial community composition. A growth curve similar to that of a bacterium grown in a batch culture was recorded. In contrast to the current perception that Gammaproteobacteria are the most important bacterial components of natural mineral water in bottles, Betaproteobacteria dominated the growing bacterial community and accounted for 80 to 98% of all bacteria detected by FISH in the late-exponential and stationary-growth phases. Using previously published and newly designed genus-specific probes, members of the betaproteobacterial genera Hydrogenophaga, Aquabacterium, and Polaromonas were found to constitute a significant proportion of the bacterial flora (21 to 86% of all bacteria detected by FISH). For the first time, key genera responsible for bacterial growth in a natural mineral water were identified by applying molecular cultivation-independent techniques.

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