scholarly journals Diversity of Bacteria Growing in Natural Mineral Water after Bottling

2005 ◽  
Vol 71 (7) ◽  
pp. 3624-3632 ◽  
Author(s):  
Alexander Loy ◽  
Wolfgang Beisker ◽  
Harald Meier
Keyword(s):  
Bacterial Community ◽  
Bacterial Growth ◽  
Mineral Water ◽  
Bacterial Community Composition ◽  
Bacterial Flora ◽  
Significant Proportion ◽  
Mineral Waters ◽  
Rrna Gene ◽  
Natural Mineral ◽  
Natural Mineral Water

ABSTRACT Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bottling. Seven 16S rRNA gene libraries, comprising 108 clones in total, were constructed from water samples taken at various days after bottling and from two different bottle sizes. Sequence analyses identified 11 operational taxonomic units (OTUs), all but one affiliated with the betaproteobacterial order Burkholderiales (6 OTUs) or the class Alphaproteobacteria (4 OTUs). Fluorescence in situ hybridization (FISH) was applied in combination with DAPI (4′,6′-diamidino-2-phenylindole) staining, viability staining, and microscopic counting to quantitatively monitor changes in bacterial community composition. A growth curve similar to that of a bacterium grown in a batch culture was recorded. In contrast to the current perception that Gammaproteobacteria are the most important bacterial components of natural mineral water in bottles, Betaproteobacteria dominated the growing bacterial community and accounted for 80 to 98% of all bacteria detected by FISH in the late-exponential and stationary-growth phases. Using previously published and newly designed genus-specific probes, members of the betaproteobacterial genera Hydrogenophaga, Aquabacterium, and Polaromonas were found to constitute a significant proportion of the bacterial flora (21 to 86% of all bacteria detected by FISH). For the first time, key genera responsible for bacterial growth in a natural mineral water were identified by applying molecular cultivation-independent techniques.

The bacterial flora of non-carbonated, natural mineral water from the springs to reservoir and glass and plastic bottles

1990 ◽  
Vol 11 (1) ◽  
pp. 51-71 ◽  
Author(s):  
Thomas Bischofberger ◽  
S.K. Cha ◽  
R. Schmitt ◽  
Bärbel König ◽  
W. Schmift-Lorenz
Keyword(s):  
Mineral Water ◽  
Bacterial Flora ◽  
Natural Mineral ◽  
Natural Mineral Water

scholarly journals The bacteriological quality of bottled natural mineral waters

1987 ◽  
Vol 99 (2) ◽  
pp. 439-443 ◽  
Author(s):  
P. R. Hunter ◽  
S. H. Burge
Keyword(s):  
Mineral Water ◽  
Mineral Waters ◽  
Gram Positive ◽  
Natural Mineral ◽  
Gram Negative ◽  
Point Of Sale ◽  
Natural Mineral Water ◽  
Bacteriological Quality ◽  
Gram Positive Cocci

SUMMARYFifty-eight bottles of natural mineral water, taken from the point of sale, were bacteriologically examined. No coliforms orAeromonas sp.were isolated from any sample. High total bacterial counts were found particularly in the still waters. Most of the organisms isolated in the total counts were Gram-negative rods, but Gram-positive organisms were also isolated. Gram-positive cocci were further identified, some of which were known human commensals suggesting contamination of the waters prior to bottling.

scholarly journals THE BACTERIAL FLORA IN BOTTLED NATURAL MINERAL WATER SOLD IN ITALY

1998 ◽  
Vol 21 (3) ◽  
pp. 175-185 ◽  
Author(s):  
S. MASSA ◽  
M. FANELLI ◽  
M.T. BRIENZA ◽  
M. SINIGAGLIA
Keyword(s):  
Mineral Water ◽  
Bacterial Flora ◽  
Natural Mineral ◽  
Natural Mineral Water

scholarly journals Physicochemical and microbiological evaluation of natural mineral water produced in Mato Grosso State, Brazil

2020 ◽  
Vol 3 (1) ◽  
pp. 32
Author(s):  
Ilza Conceição Tomaselli Ribeiro ◽  
Adriana Paiva de Oliveira ◽  
Andressa De Souza David ◽  
Kamila Cristina Oliveira ◽  
Marcia Helena Scabora ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Mineral Water ◽  
Mineral Waters ◽  
Total Alkalinity ◽  
Coliform Bacteria ◽  
Mato Grosso ◽  
Natural Mineral ◽  
Natural Mineral Water ◽  
Industrialization Process

The aim of this work was to evaluate the physicochemical and microbiological properties of natural mineral waters bottled in 20 liter containers produced in Mato Grosso - Brazil. For this study, three lots of eight brands produced in the state and commercialized in Cuiabá were analyzed. The physicochemical parameters determined were pH, electrical conductivity, total dissolved solids, total alkalinity, metals, ammonium, anions and glyphosate, and the measurements were made according to the Adolfo Lutz Institute and Standard Methods for the Examination of Water and Wastewater. Chromogenic enzymatic substrates were used to determine Coliform bacteria and filtration with a filtering membrane was used for the Pseudomonas aeruginosa count. The results were compared with national and international legislation on bottled water and the labeled values. All physicochemical parameters, except for pH, showed concordant results with maximum values allowed by Brazilian and international law for the quality of bottled natural mineral waters. Six samples were investigated for the presence of coliforms and P. aeruginosa at 35 ºC. The results suggested possible contamination and failure during the industrialization process and the requirement for inspection during collection, manufacturing, labeling and commercialization of the natural mineral water bottled in 20 liter containers.

scholarly journals Lithium in Portuguese Bottled Natural Mineral Waters—Potential for Health Benefits?

2020 ◽  
Vol 17 (22) ◽  
pp. 8369
Author(s):  
Maria Orquídia Neves ◽  
José Marques ◽  
Hans G.M. Eggenkamp
Keyword(s):  
Drinking Water ◽  
Inductively Coupled Plasma ◽  
Mineral Water ◽  
Tap Water ◽  
Health Benefits ◽  
Epidemiological Studies ◽  
Mineral Waters ◽  
Natural Mineral ◽  
Natural Mineral Water ◽  
Inductively Coupled

There is increasing epidemiologic and experimental evidence that lithium (Li) exhibits significant health benefits, even at concentrations lower than the therapeutic oral doses prescribed as treatment for mental disorders. The aim of this study is to determine the content of Li in 18 brands of bottled natural mineral waters that are available on the Portuguese market and from which the sources are found within the Portuguese territory, to provide data for Li intake from drinking water. Analyses of Li were performed by inductively coupled plasma-mass spectrometry. The results indicate highly different Li concentrations in natural mineral waters: one group with low Li concentrations (up to 11 µg Li/L) and a second group with Li concentrations higher than 100 µg/L. The highest Li concentrations (>1500 µg Li/L) were observed in the highly mineralized Na-HCO3 type waters that are naturally carbonated (>250 mg/L free CO2). As a highly bioavailable source for Li dietary intake these natural mineral waters have potential for Li health benefits but should be consumed in a controlled manner due to its Na and F− contents. The consumption of as little as 0.25 L/day of Vidago natural mineral water (2220 µg Li/L), can contribute up to 50% of the proposed daily requirement of 1 mg Li/day for an adult (70 kg body weight). In future, Li epidemiological studies that concern the potential Li effect or health benefits from Li in drinking water should consider not only the Li intake from tap water but also intake from natural mineral water that is consumed in order to adjust the Li intake of the subjects.

scholarly journals Assessing natural mineral water microbiology quality in the absence of cultivable pathogen bacteria

2018 ◽  
Vol 16 (3) ◽  
pp. 425-434 ◽  
Author(s):  
Michele Totaro ◽  
Beatrice Casini ◽  
Paola Valentini ◽  
Mario Miccoli ◽  
Pier Luigi Lopalco ◽  
...  
Keyword(s):  
Mineral Water ◽  
Nontuberculous Mycobacteria ◽  
Mineral Waters ◽  
Acanthamoeba Polyphaga ◽  
Natural Mineral ◽  
Natural Mineral Water ◽  
Cultivable Bacteria ◽  
Water Microbiology ◽  
Microbiological Indicators

Abstract Italian Directives recommend the good quality of natural mineral waters but literature data assert a potential risk from microorganisms colonizing wellsprings and mineral water bottling plants. We evaluated the presence of microorganisms in spring waters (SW) and bottled mineral waters (BMW) samples. Routine microbiological indicators, additional microorganisms like Legionella spp., Nontuberculous mycobacteria (NTM) and amoebae (FLA) were assessed in 24 SW and 10 BMW samples performing cultural and molecular methods. In 33 out of 34 samples, no cultivable bacteria ≥10 CFU/L was found. Cultivable FLA were detected in 50% of water samples. qPCR showed the presence of Legionella qPCR units in 24% of samples (from 1.1 × 102 to 5.8 × 102 qPCR units/L) and NTM qPCR units in 18% of samples (from 1 × 102 to 1 × 105 qPCR units/L). Vermamoeba vermiformis and Acanthamoeba polyphaga were recovered respectively in 70% of BMW samples (counts from 1.3 × 103 to 1.2 × 105 qPCR units/L) and 42% of SW samples (from 1.1 × 103 to 1.3 × 104 qPCR units/L). Vahlkampfia spp. was detected in 42% of SW and 70% of BMW samples (from 1.2 × 103 to 1.2 × 105 qPCR units/L). Considering the presence of FLA, we underline the importance of a wider microbiological risk assessment in natural mineral waters despite the absence of cultivable bacteria.

scholarly journals Molecular Typing of Aeromonas Isolates in Natural Mineral Waters

2003 ◽  
Vol 69 (1) ◽  
pp. 697-701 ◽  
Author(s):  
P. Villari ◽  
M. Crispino ◽  
P. Montuori ◽  
S. Boccia
Keyword(s):  
Mineral Water ◽  
Mineral Waters ◽  
Cytotoxic Activities ◽  
Molecular Heterogeneity ◽  
Molecular Fingerprinting ◽  
Natural Mineral ◽  
Natural Mineral Water ◽  
Aeromonas Caviae ◽  
Water Wells ◽  
Clonal Identity

ABSTRACT A total of 103 isolates of Aeromonas spp. were obtained over a 3-year period from a natural mineral water and from surface streams located within the boundaries of the watershed of the natural mineral water wells and were typed by macrorestriction analysis of genomic DNA with XbaI and by pulsed-field gel electrophoresis. All Aeromonas caviae isolates from the natural mineral water belonged to the same clone, and an analogous clonal identity was found among Aeromonas hydrophila isolates. These two clones expressed no hemolytic or cytotoxic activities. Aeromonas isolates from surface waters showed high molecular heterogeneity and were not related to the clones found in the natural mineral water. The presence of aeromonads chronically found in the natural mineral water was a likely consequence of a localized development of a biofilm, with no exogenous contamination of the aquifer. Molecular fingerprinting of drinking water isolates is a useful tool in explaining possible reasons for bacterial occurrences.

scholarly journals THE EFFECT OF SELENIUM MODIFIED CARBON DIOXIDE MINERAL WATERS OF PYATIGORSK FIELD ON SOME SELENIUM DEPENDED SYSTEMS

2018 ◽  
Vol 17 (1) ◽  
pp. 32-36
Author(s):  
Anna Viktorovna Abramtsova ◽  
N. V Efimenko ◽  
A. S Kaysinova ◽  
L. V Polyakova
Keyword(s):  
Carbon Dioxide ◽  
Thyroid Gland ◽  
Linear Regression ◽  
Mineral Water ◽  
Mineral Waters ◽  
Selenium Nanoparticles ◽  
Histological Structure ◽  
Natural Mineral ◽  
Natural Mineral Water ◽  
Healthy Control

The influence of course intake of the natural mineral water of the Pyatigorsk source, modified by selenium nanoparticles at a dosage of 40 μg/kg, on the level of thyroid hormones, insulin, cortisol and dehydroepiandrosterone, including depending on the method of drinking animals (free drinking and intragastric) was studied in rats. The histological structure of the thyroid gland during drinking with mineral water corresponded to that of healthy control animals, but in the group that received water intragastrically through the probe, the function of thyrocytes and levels of triiodothyronine and dehydroepiandrosterone in the blood were reduced. The courses of the mineral water modified by selenium nanoparticles, according to the data of multiple linear regression, contributed to the activation of the thyroid system.

scholarly journals Effects of Simulated Nitrogen Deposition on the Bacterial Community of Urban Green Spaces

2021 ◽  
Vol 11 (3) ◽  
pp. 918
Author(s):  
Lingzi Mo ◽  
Augusto Zanella ◽  
Xiaohua Chen ◽  
Bin Peng ◽  
Jiahui Lin ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Diversity ◽  
Negative Impact ◽  
Bacterial Community Composition ◽  
N Deposition ◽  
Soil Bacterial Community ◽  
Rrna Gene ◽  
Soil Dna ◽  
Urban Green ◽  
Soil Bacterial

Continuing nitrogen (N) deposition has a wide-ranging impact on terrestrial ecosystems. To test the hypothesis that, under N deposition, bacterial communities could suffer a negative impact, and in a relatively short timeframe, an experiment was carried out for a year in an urban area featuring a cover of Bermuda grass (Cynodon dactylon) and simulating environmental N deposition. NH4NO3 was added as external N source, with four dosages (N0 = 0 kg N ha−2 y−1, N1 = 50 kg N ha−2 y−1, N2 = 100 kg N ha−2 y−1, N3 = 150 kg N ha−2 y−1). We analyzed the bacterial community composition after soil DNA extraction through the pyrosequencing of the 16S rRNA gene amplicons. N deposition resulted in soil bacterial community changes at a clear dosage-dependent rate. Soil bacterial diversity and evenness showed a clear trend of time-dependent decline under repeated N application. Ammonium nitrogen enrichment, either directly or in relation to pH decrease, resulted in the main environmental factor related to the shift of taxa proportions within the urban green space soil bacterial community and qualified as a putative important driver of bacterial diversity abatement. Such an impact on soil life induced by N deposition may pose a serious threat to urban soil ecosystem stability and surrounding areas.

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