scholarly journals Produksi Asam Laktat dari Fermentasi Limbah Cair Olahan Kelapa dengan Variasi Konsentrasi Inokulum Lactobacillus acidophilus

2020 ◽  
Vol 5 (4) ◽  
Author(s):  
Ratna Ratna ◽  
Ulfa Triovanta ◽  
Darwin Darwin
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Acidophilus ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Liquid Waste ◽  
Total Suspended Solid ◽  
Strong Relationship ◽  
Suspended Solid ◽  
Continuous Systems ◽  
Inoculum Concentration

The aim of this research is to study the fermentation technique of coconut processed liquid waste by varying the inoculum concentration of Lactobacillus acidophilus bacteria for lactic acid production. This research was carried out in several steps, namely the first step was the inoculum cultivation process of Lactobacillus acidophilus bacteria, the second step was the fermentation process of coconut water with variations in the concentration of Lactobacillus acidophilus bacteria inoculum, namely 5%, 10% and 20%. Analysis of lactic acid products, pH, glucose, Total Dissolved Solid (TDS), Total Suspended Solid (TSS) and Volatile Suspended Solid (VSS). The variation in the concentration of Lactobacillus acidophilus bacteria inoculum 20% is the most optimum to produce total lactic acid for batch and continuous systems. The results of the correlation analysis for inoculum variations of 5%, 10% and 20% had a strong relationship with lactic acid production, decreased pH and decreased glucose levels. The greater the inoculum concentration, the greater the decrease in the TDS, TSS and VSS content.

scholarly journals Correlation of Some Substrate Parameters in Growing Lactobacillus acidophilus on Vegetable and Fruit Cocktail Juices

2010 ◽  
Vol 67 (2) ◽  
Author(s):  
Carmen Leane NICOLESCU ◽  
Lavinia Claudia BURULEANU
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Acidophilus ◽  
Acid Production ◽  
Sugar Content ◽  
Lactic Acid Production ◽  
Biomass Accumulation ◽  
Reducing Sugar ◽  
Fruit Juices ◽  
Cell Multiplication ◽  
Microbiological Methods

Using Lactobacillus acidophilus stains is a challenge in producing lactic acid fermented vegetable and fruit juices. There were analysed the correlation between the most important physico-chemical parameters of the substrate and the bacterial biomass accumulation in two vegetable and fruit juices. The data were collected during a 48 hour lactic acid fermentation using usually chemical and microbiological methods. There was analysed the correlation between the reducing sugar content and the lactic acid production, the correlation between the pH and lactic acid production, the correlation between the reducing sugar of the substrate and the microbial biomass accumulation and also between the lactic acid production and biomass accumulation. These correlations were analysed using simple regression on scatter plots. They were best fitted by the polynomial equation where the highest R2 were calculated. The two last correlations had large differences between the two experimental batches so that regression is not satisfactory to describe them. If all parameters were considered using the multiple regression, the correlation had a medium value because the cell multiplication of the bacteria Lactobacillus acidophilus in vegetable and fruit juices is influenced by many other environmental parameters. These had a large influence because juices are not the natural medium for this kind of lactic acid bacteria.

scholarly journals Effects of nanobubble water on the growth of Lactobacillus acidophilus 1028 and its lactic acid production

RSC Advances ◽  
2019 ◽  
Vol 9 (53) ◽  
pp. 30760-30767 ◽  
Author(s):  
Zitao Guo ◽  
Xuezhi Wang ◽  
Hanxiao Wang ◽  
Bo Hu ◽  
Zhongfang Lei ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Growth ◽  
Lactobacillus Acidophilus ◽  
Acid Production ◽  
Culture Medium ◽  
Lactic Acid Production

Nanobubbles (NBs) in the culture medium may offer a new way of accelerating bacterial growth in the lag and logarithmic phases.

scholarly journals Anaerobic Acidification of Coconut Water Waste by Lactobacillus acidophilus Culture for Biotechnological Production of Lactic Acid

2019 ◽  
Vol 67 (6) ◽  
pp. 1433-1440
Author(s):  
Darwin ◽  
Ulfa Triovanta ◽  
Ridho Rinaldi ◽  
Atmadian Pratama
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Acidophilus ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Batch Process ◽  
Coconut Water ◽  
Biotechnological Production ◽  
Optimal Production ◽  
Anaerobic Acidification ◽  
Water Waste

The biotechnological production of lactic acid could be carried out via anaerobic acidification process. In order to achieve an optimal production of lactic acid, the role of inoculum would be essential. The current study aimed to investigate as well as evaluate the effect of inoculum concentration on the anaerobic acidification of coconut water waste for the production of lactic acid. Results showed that the addition of 20% inoculums to the reactor fermenting coconut water waste was sufficient for the optimal production of lactic acid. In the batch process anaerobic acidification of coconut water waste inoculated with 20% inoculums of Lactobacillus acidophilus culture had the yield of lactic acid production, which was about 1.62 mmol lactic acid/mmol glucose while under the continuous operation the yield of lactic acid production obtained, was about 1.15 mmol lactic acid/mmol glucose. During the acidification process in both batch and continuous modes pH dropped significantly from 5.1 to 3.7.

scholarly journals Evaluation of Growth, Viability, Lactic Acid Production and Anti-Infective Effects of Lacticaseibacillus rhamnosus ATCC 9595 in Bacuri Juice (Platonia insignis)

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 603
Author(s):  
Yasmim Costa Mendes ◽  
Gabrielle Pereira Mesquita ◽  
Gabrielle Damasceno Evangelista Costa ◽  
Ana Carolina Barbosa da Silva ◽  
Ester Gouveia ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Protective Effects ◽  
Organic Acid Production ◽  
Inoculum Concentration ◽  
E Coli ◽  
Probiotic Product ◽  
Pre Treatment ◽  
Tenebrio Molitor Larvae

Fruit juices have been emerging as excellent vehicles for development of probiotic products due to their nutritional properties and presence of bioactive compounds. This work evaluated the growth and viability of Limosilactobacillus fermentum ATCC 23271 and Lacticaseibacillus rhamnosus ATCC 9595 in bacuri juice (Platonia insignis Mart., Clusiaceae). Both strains were able to grow in bacuri juice, without any supplementation. Viability was kept after 28 days of storage; however, growth was significantly higher for L. rhamnosus ATCC 9595 (7.40 ± 0.04 Log CFU/mL). Following this, the effects of bacterial inoculum and pulp concentration on growth and lactic acid production by L. rhamnosus ATCC 9595 were investigated using a central composite rotational design. The inoculum concentration was the main factor for obtaining the most favorable relation between growth and organic acid production (G/pH ratio). Among the tested conditions, those used in assay 6 allowed the best G/pH ratio (2.13) and higher lactic acid production (4.14 g/L). In these conditions, L. rhamnosus ATCC 9595 grown in bacuri juice showed the same resistance towards acidification or addition of lysozyme than when cultivated in MRS. Finally, the anti-infective effects of fermented and non-fermented juices were analyzed using Tenebrio molitor larvae infected by enteroaggregative Escherichia coli 042. The pre-treatment with supernatants of both fermented and non-fermented juices significantly increased the survival of E. coli-infected larvae. However, only the L. rhamnosus-fermented juice had protective effects when inoculated 2 h after infection. Collectively, the results obtained in this research allowed the basis for the development of a non-dairy probiotic product from bacuri juice.

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