Nutraceutical Properties of Important Weeds in India

The group of plants, apart from the forest trees, agricultural, horticultural crop and medicinal plants, certain weed plants also play important role to formulate the qualitative and quantitative food and health supplements. These plants may be very specific in producing certain compound to heal the specific ailment chronically or serve as a good nutritive food supplement. There are numbers of crop species have been fortified with multi-nutrients or with the particular nutrient supplements e.g. Rice, Wheat, Maize, Sweet Potato. Instead, weeds remained underutilized and un-exploited and also treated as unwanted species. The nutritive compounds available in these plants such as polyphenols and vitamins can be the extraction from different plant portion to formulate the fortified food or curing medicine. The weed species prevailing in the nature are one of the major sources of dietary and health supplements for humans and animals since past i.e. Amaranthus lividus, Chenopodium album and many more weed species. There is still much to explore the Indian ecologic components present in wild form of flora. Hence, this chapter will majorly contribute to highlight nutritive nutaceurtical and edible weeds prevailing in Indian ecosystems.

Knowledge, Use and Practices of Licensed Dietitians Related to Dietary Supplements in Lebanon

Objective To evaluate Lebanese licensed dietitians’ (LDs) knowledge, prevalence of use and recommendation of dietary supplements (DS), and their associated factors. Design Cross-sectional survey. Setting Dietitians across Lebanon were contacted through the telephone and were asked to participate in the study. An online self-administered questionnaire was designed and sent to dietitians either by email or by WhatsApp, between March 4th and May 4th, 2020. Participants This study included 319 dietitians randomly selected from the dietitians’ list that was provided by Lebanese Ministry of Public Health. Results Around 75% of dietitians had a knowledge score above 50%. Overall, 73.7% of them have used DS and 46.1% have recommended them. Higher knowledge score was associated with less years of experience, using scientific articles as source of information on DS, and participating in research. Resorting to pharmacists was significantly associated with both nutrient supplements (NS) and herbal supplements’ (HS) use, whereas referring to health food stores was associated with HS use only. NS recommendation to patients was associated with personal NS use (OR=3.38, P<0.001), considering pharmacists as a source of information on DS (OR=2.29, P=0.01) and discussing DS with patients (OR=3.82, P=0.01). Having personally used HS (OR=12.23, P<0.001) and having discussed DS with patients (OR=8.51, P=0.01) increased the likelihood of recommending HS. Conclusions A proper DS education, the elaboration of national scientific guidelines and the implementation of concise laws regarding the regulations of DS would play a crucial role in supporting dietitians’ practices and improving the quality of patient care with respect to DS.

Physicochemical Indices of Phyto Remediated Hydrocarbon Polluted Soil and the Effect of Bio-nutrient Amendment

Aim: To assess the Physicochemical indices of Phytoremediated Crude Oil polluted amended soil using grass plant Cyperus esculentus (Cyp) and Phyllanthus amarus (Phy). Study Design: The study employs experimental design, statistical analysis of the data and interpretation. Place and Duration of Study: Rivers State University demonstration farmland in Nkpolu- Oroworukwo, Mile 3 Diobu area of Port Harcourt, was used for this study. The piece of land is situated at Longitude 4°48’18.50” N and Latitude 6ᵒ58’39.12” E measuring 5.4864 m x 5.1816 m with a total area of 28.4283 square meter. Phytoremediation process monitoring lasted for 240 days; analyses were carried out monthly at 30 days’ interval. Methodology: The study was carried out on Crude Oil Polluted soil (PS) amended with bio-nutrient supplements (Spent Mushroom Substrate (SMS) and selected fungi (Aspergillus niger(AN) andMucor racemosus (MR)) used to stimulate and augment the indigenous microbial population present in a crude oil polluted soil thereby enhancing hydrocarbon reduction in pari per sue with phytoremediation (uptake of Crude oil by test plants) over a period of 240 days. Ten (10) experimental plots (two Control (Unpolluted and polluted soil without amendment) and eight polluted amended/treated plots) employing Randomized Block Design (each having dimensions: 100 x 50 x 30 cm LxBxH); formed and mapped out on agricultural soil and left fallow for 6 days before contamination on the seventh day; after which it was allowed for 21 days for proper contamination and exposure to natural environmental factors (to mimic soil crude oil spill site); thereafter nutrients/organics (biostimulating agents) and bioaugmenting organisms were applied. Baseline studies were carried out on soil profile before and after contamination, major parameters monitored and assayed were Total Petroleum Hydrocarbon (TPH) uptake by plant roots and stem, Polycyclic Aromatic Hydrocarbons (PAHs) and TPH reduction in soil. Other physicochemical properties analyzed in the soil from different plots were pH, Electrical Conductivity, Moisture Content, Total Nitrogen, Available Phosphorus, Potassium, Total Organic Carbon, Plant Height, Iron, Lead and Zinc at regular intervals; days 1, 60, 90, 120, 150, 180, 210 & 240. The rate of phytoremediation was estimated from percentage (%) uptake of Total petroleum hydrocarbon (TPH) in plant roots and stem from day 1 -240; while percentage (%) reduction of TPH and PAHs in soil was estimated from day 1 to the residual at day 240. Results: The test plants decreased significant amount of crude oil as revealed in TPH uptake in their roots and Stem. Mean amount and percentage Total Petroleum Hydrocarbon (TPH) uptake by Cyperus esculentus roots and stem were; 152.33±50.34mg/kg, 12.57±4.16% and 201.13±8.80mg/kg, 13.27±0.58% respectively; while that of Phyllanthus amarusroots and stem were 141.50±35.62mg/kg, 11.68±2.94% and 174.44±19.98mg/kg, 11.51±1.32% respectively; revealing higher Uptake of TPH in plant stem than roots. From the initial TPH contamination value of 5503.00mg/kg, it was observed that plots planted with Cyperus esculentus (TPH 5492.75±76.36mg/kg) showed higher reduction of TPH from soil than those planted with Phyllanthus amarus(TPH 5449.72±18.27mg/kg); while PAHs degradation/reduction showed a reverse trend with plots planted with Phyllanthus amarus (PAHs 28.72±2.74mg/kg; 60.46±5.77%) higher than plots planted with Cyperus esculentus s (PAHs 25.77±2.12mg/kg, 54.24±4.47%). Conclusion: Plots planted with Cyperus esculentus showed higher reduction of TPH from soil than those planted with Phyllanthus amarus while PAHs degradation/reduction in plots planted with Phyllanthus amarus was higher than plots planted with Cyperus esculentus. TPH uptake was higher in plant stems than roots; more so, plots amended with nutrient supplements showed significant higher percentage reduction in hydrocarbon in the polluted soil than unamended polluted soil. It is therefore recommended that Cyperus esculentus is a suitable plant species for phytoremediation of crude oil contaminated soil with high TPH value while Phyllanthus amarusis the best option in phytoremediation of polluted soil with high PAHs value, both in combination with bio-nutrient supplement.

The Antiviral Activity of Bacterial, Fungal, and Algal Polysaccharides as Bioactive Ingredients: Potential Uses for Enhancing Immune Systems and Preventing Viruses

Viral infections may cause serious human diseases. For instance, the recent appearance of the novel virus, SARS-CoV-2, causing COVID-19, has spread globally and is a serious public health concern. The consumption of healthy, proper, functional, and nutrient-rich foods has an important role in enhancing an individual's immune system and preventing viral infections. Several polysaccharides from natural sources such as algae, bacteria, and fungi have been considered as generally recognized as safe (GRAS) by the US Food and Drug Administration. They are safe, low-toxicity, biodegradable, and have biological activities. In this review, the bioactive polysaccharides derived from various microorganisms, including bacteria, fungi, and algae were evaluated. Antiviral mechanisms of these polysaccharides were discussed. Finally, the potential use of microbial and algal polysaccharides as an antiviral and immune boosting strategy was addressed. The microbial polysaccharides exhibited several bioactivities, including antioxidant, anti-inflammatory, antimicrobial, antitumor, and immunomodulatory activities. Some microbes are able to produce sulfated polysaccharides, which are well-known to exert a board spectrum of biological activities, especially antiviral properties. Microbial polysaccharide can inhibit various viruses using different mechanisms. Furthermore, these microbial polysaccharides are also able to modulate immune responses to prevent and/or inhibit virus infections. There are many molecular factors influencing their bioactivities, e.g., functional groups, conformations, compositions, and molecular weight. At this stage of development, microbial polysaccharides will be used as adjuvants, nutrient supplements, and for drug delivery to prevent several virus infections, especially SARS-CoV-2 infection.

Augmenting Whole Recombinant Antibody Production in HEK293E Cells: Optimizing Transfection and Culture Parameters

Optimizing recombinant antibody production is important for cost-effective therapeutics and diagnostics. With downstream impact on commercialization, higher productivity is highly sought after beyond laboratory scales, where efficient production can also accelerate antibody characterizations and investigations. Using HEK293E cells as the base model for mammalian antibody production, various transfection and culture parameters were systematically analyzed using antibody light chain production before applying them onto whole antibody production. Transfection parameters investigated include seeding cell density, the concentration of the transfection reagent and DNA, complexation time, temperature, and volume, as well as culture parameters such as media replacement, serum deprivation, media volume, post-transfection harvest, incubation temperature, and common nutrient supplements. Incorporating the most optimized parameters, Pertuzumab κ-chain only and whole recombinant Pertuzumab antibody production were found to increase by 452 % and 252 % respectively, that can be used to guide future cost-effective transient antibody production with the potential for further scaling up.

Small-Quantity Lipid-Based Nutrient Supplements Increase Infants’ Plasma Essential Fatty Acid Levels in Ghana and Malawi: A Secondary Outcome Analysis of the iLiNS-DYAD Randomized Trials

Introduction Small-quantity lipid-based nutrient supplements (SQ-LNSs) may influence infants’ plasma fatty acid (FA) profiles, which could be associated with short- and long-term outcomes. Objectives We aimed to determine the impact of SQ-LNS consumption on infants’ plasma FA profiles in Ghana and Malawi. Methods Ghanaian (n = 1320) and Malawian (n = 1391) women ≤ 20 wk pregnant were assigned to consume daily: 60 mg iron and 400 µg folic acid until delivery (IFA group); or multiple micronutrients until 6 mo postpartum (MMN); or SQ-LNSs (∼7.8 Linoleic acid: α-Linolenic acid ratio) until 6 mo postpartum (LNS). LNS group infants received SQ-LNS from 6 to 18 mo of age. We compared infant plasma FAs by intervention group in sub-samples (n = 379, Ghana; n = 442, Malawi) at 6 and 18 mo using ANOVA and Poisson regression models. Main outcomes were mean % composition (%C, % FAs by weight) of α-linolenic (ALA), linoleic (LA), eicosapentaenoic (EPA), docosahexaenoic (DHA), and arachidonic (AA) acids. Results At 6 mo, LNS infants had greater mean ± SD ALA %C in Ghana (0.23 ± 0.08 vs IFA, 0.21 ± 0.06; MMN, 0.21 ± 0.07; P = 0.034) and Malawi (0.42 ± 0.16 vs IFA, 0.38 ± 0.15; MMN, 0.38 ± 0.14; P = 0.034) and greater AA (6.25 ± 1.24 vs IFA, 6.12 ± 1.13; MMN, 5.89 ± 1.24; P = 0.049) in Ghana. At 18 mo, LNS infants had a tendency towards greater ALA (0.32 ± 0.16 vs IFA, 0.24 ± 0.08; MMN, 0.24 ± 0.10; P = 0.06) and LA (27.8 ± 3.6 vs IFA, 26.9 ± 2.9; MMN, 27.0 ± 3.1; P = 0.06) in Ghana, and greater ALA (0.45 ± 0.18 vs IFA, 0.39 ± 0.18; MMN, 0.39 ± 0.18; P &lt; 0.001) and LA (29.7 ± 3.5 vs IFA, 28.7 ± 3.3; MMN, 28.6 ± 3.4; P = 0.011) in Malawi. The prevalence of ALA below the population-specific 10th percentile was lower in the LNS group compared to the MMN group, but not the IFA group. Groups did not differ significantly in plasma EPA or DHA levels. Conclusion SQ-LNS increases infants’ plasma essential FA levels in Ghana and Malawi, which may have implications for health and developmental outcomes. Clinicaltrials.gov identifiers: NCT00970866; NCT01239693. Clinical Trial Registry Number; website: NCT00970866, NCT01239693; ClinicalTrials.gov

Therapeutic Potential of Nutraceuticals and Dietary Supplements in the Prevention of Viral Diseases: A Review

Nowadays, despite enormous scientific advances, viral diseases remain the leading cause of morbidity worldwide, and their potential to spread is escalating, eventually turning into pandemics. Nutrition can play a major role in supporting the immune system of the body and for the optimal functioning of the cells of the immune system. A healthy diet encompassing vitamins, multi-nutrient supplements, functional foods, nutraceuticals, and probiotics can play a pivotal role in combating several viral invasions in addition to strengthening the immune system. This review provides comprehensive information on diet-based scientific recommendations, evidence, and worldwide case studies in light of the current pandemic and also with a particular focus on virus-induced respiratory tract infections. After reviewing the immune potential of nutraceuticals based on the lab studies and on human studies, it was concluded that bioactive compounds such as nutraceuticals, vitamins, and functional foods (honey, berries, etc.) with proven antiviral efficacy, in addition to pharmaceutical medication or alone as dietary supplements, can prove instrumental in treating a range of virus-induced infections in addition to strengthening the immune system. Milk proteins and peptides can also act as adjuvants for the design of more potent novel antiviral drugs.

SUPPORTIVE THERAPY: AN OPTION TO ENHANCE HOST IMMUNITY AGAINST COVID-19

The threat posed by COVID 19 outbreak, which is considered to be a global pandemic, is immeasurably affecting all the communities worldwide. COVID-19 is a zoonotic disease, which can affect birds, humans and, other animals. The emergence of this pandemic has been creating a tragic situation worldwide by affecting more people through human-human transmission. The burden (both healthwise and economic) placed by the disease is so huge that any measures to improve the current situation, to fasten up the recovery of already affected patients and, to reduce the risk of death and health deterioration should be considered. Vaccination, being the hope in the scenario, helps in preventing the condition to an extent, but in the absence of availability of a proper drug regimen to fight off COVID 19, the requirement of the need to find a system to control the severity of the infection is a necessity Nutritional supplementation helps in boosting up the immune system especially, vitamins like vitamin C, Vitamin D, Zinc, Omega 3 fatty acids, etc. They also exhibit established immunomodulatory, antiviral as well as anti-inflammatory effects. Pieces of evidence have also highlighted the importance of supportive therapy using nutrient supplements in covid patients as it helps in prominent decreasing of SARS CoV2 load of the virus and also significantly reduces the hospitalization period. Hence the nutritional levels of each of the infected person must be assessed before initiating the anti-viral therapy. The search criteria used were PubMed, Medscape, google scholar, etc. The keywords used to search were COVID 19 Supportive therapy, Vitamin D, Vitamin C, Nutrient supplementation, Host immunity, etc. The range of years is between 1978 and 2021.