scholarly journals Characterization Of A Single Pixel Beta Detector For Guidance In Breast-Conserving Surgery

2021 ◽  
Author(s):  
Amritpal Singh
Keyword(s):  
In Silico ◽  
Breast Conserving Surgery ◽  
Beta Particle ◽  
Resection Margins ◽  
Acquisition Time ◽  
Detector Response ◽  
In Silico Study ◽  
Detection Ratio ◽  
Single Pixel ◽  
Gamma Detection

Breast-conserving surgery (BCS) is a challenging surgical procedure due to the lack of intraoperative image guidance available to surgeons. One potential method of intra-operative guidance would be radio-guided surgery with adiopharmaceutical emitting beta particles. In this thesis, a single pixel beta sensitive detector was constructed and characterized for intra-operative guidance during BCS. The thickness of the scintillation element of the detector was optimized to obtain a superior beta to gamma detection ratio. A computer model of the detector response was derived from an empirically measured, two-dimensional (2D) detector response. An in silico study evaluated whether the novel single pixel beta detector could detect less than 1 mm² deposits of cancer at the cut edge of the surgically excised cancerous tissue, with a sensitivity and specificity of 95%. A thickness of 0.5 mm for a CaF₂(Eu) scintillator was found to be optimal for a beta to gamma detection ratio. Additionally, according to an in silico study it is expected that with an acquisition time of 30 seconds, a tumour-to-background ratio of 5 or higher, and a normal breast tissue activity of 1.69 kBq/ml, detection of cancerous deposits of less than 1 mm² is possible. The result of this thesis demonstrate that radio-guided BCS, with a CaF₂(Eu) scintillation beta particle detector, can intra-operatively assess the tumour margin involvement, which would help surgeons in determining resection margins.

scholarly journals Characterization Of A Single Pixel Beta Detector For Guidance In Breast-Conserving Surgery

2021 ◽  
Author(s):  
Amritpal Singh
Keyword(s):  
In Silico ◽  
Breast Conserving Surgery ◽  
Beta Particle ◽  
Resection Margins ◽  
Acquisition Time ◽  
Detector Response ◽  
In Silico Study ◽  
Detection Ratio ◽  
Single Pixel ◽  
Gamma Detection

Breast-conserving surgery (BCS) is a challenging surgical procedure due to the lack of intraoperative image guidance available to surgeons. One potential method of intra-operative guidance would be radio-guided surgery with adiopharmaceutical emitting beta particles. In this thesis, a single pixel beta sensitive detector was constructed and characterized for intra-operative guidance during BCS. The thickness of the scintillation element of the detector was optimized to obtain a superior beta to gamma detection ratio. A computer model of the detector response was derived from an empirically measured, two-dimensional (2D) detector response. An in silico study evaluated whether the novel single pixel beta detector could detect less than 1 mm² deposits of cancer at the cut edge of the surgically excised cancerous tissue, with a sensitivity and specificity of 95%. A thickness of 0.5 mm for a CaF₂(Eu) scintillator was found to be optimal for a beta to gamma detection ratio. Additionally, according to an in silico study it is expected that with an acquisition time of 30 seconds, a tumour-to-background ratio of 5 or higher, and a normal breast tissue activity of 1.69 kBq/ml, detection of cancerous deposits of less than 1 mm² is possible. The result of this thesis demonstrate that radio-guided BCS, with a CaF₂(Eu) scintillation beta particle detector, can intra-operatively assess the tumour margin involvement, which would help surgeons in determining resection margins.

In Silico Study of Structural and Geometrical Requirements of Natural Sesquiterpene Lactones with Trypanocidal Activity

2013 ◽  
Vol 13 (10) ◽  
pp. 1407-1414 ◽  
Author(s):  
L. Fabian ◽  
V. Sulsen ◽  
F. Frank ◽  
S. Cazorla ◽  
E. Malchiodi ◽  
...  
Keyword(s):  
In Silico ◽  
Sesquiterpene Lactones ◽  
Trypanocidal Activity ◽  
In Silico Study

In Silico Study of 1, 4 Alpha Glucan Branching Enzyme and Substrate Docking Studies

2020 ◽  
Vol 17 (1) ◽  
pp. 40-50
Author(s):  
Farzane Kargar ◽  
Amir Savardashtaki ◽  
Mojtaba Mortazavi ◽  
Masoud Torkzadeh Mahani ◽  
Ali Mohammad Amani ◽  
...  
Keyword(s):  
Phylogenetic Tree ◽  
In Silico ◽  
Alpha Helix ◽  
In Silico Analysis ◽  
Glycogen Storage ◽  
Docking Studies ◽  
Random Coil ◽  
Special Topic ◽  
Industrial Biotechnology ◽  
In Silico Study

Background: The 1,4-alpha-glucan branching protein (GlgB) plays an important role in the glycogen biosynthesis and the deficiency in this enzyme has resulted in Glycogen storage disease and accumulation of an amylopectin-like polysaccharide. Consequently, this enzyme was considered a special topic in clinical and biotechnological research. One of the newly introduced GlgB belongs to the Neisseria sp. HMSC071A01 (Ref.Seq. WP_049335546). For in silico analysis, the 3D molecular modeling of this enzyme was conducted in the I-TASSER web server. Methods: For a better evaluation, the important characteristics of this enzyme such as functional properties, metabolic pathway and activity were investigated in the TargetP software. Additionally, the phylogenetic tree and secondary structure of this enzyme were studied by Mafft and Prabi software, respectively. Finally, the binding site properties (the maltoheptaose as substrate) were studied using the AutoDock Vina. Results: By drawing the phylogenetic tree, the closest species were the taxonomic group of Betaproteobacteria. The results showed that the structure of this enzyme had 34.45% of the alpha helix and 45.45% of the random coil. Our analysis predicted that this enzyme has a potential signal peptide in the protein sequence. Conclusion: By these analyses, a new understanding was developed related to the sequence and structure of this enzyme. Our findings can further be used in some fields of clinical and industrial biotechnology.

In Silico Study of Ethylene Biosynthesis: Seeking New Effectors of ACC Synthase and ACC Oxidase

2016 ◽  
Vol 11 (3) ◽  
pp. 346-356
Author(s):  
Nada Ayadi ◽  
Sarra Aloui ◽  
Rabeb Shaiek ◽  
Oussama Rokbani ◽  
Faten Raboud ◽  
...  
Keyword(s):  
In Silico ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Ethylene Biosynthesis ◽  
In Silico Study

In Silico Identification of a Potent Arsenic Based Approved Drug Darinaparsin against SARS-CoV-2: Inhibitor of RNA Dependent RNA polymerase (RdRp) and Essential Proteases

2020 ◽  
Vol 20 ◽  
Author(s):  
Trinath Chowdhury ◽  
Gourisankar Roymahapatra ◽  
Santi M. Mandal
Keyword(s):  
Rna Polymerase ◽  
Viral Entry ◽  
In Silico ◽  
Rna Dependent Rna Polymerase ◽  
Replication Complex ◽  
In Silico Study ◽  
Life Threatening ◽  
In Vivo Analysis ◽  
3Cl Protease

Background: COVID-19 is a life threatening novel corona viral infection to our civilization and spreading rapidly. Terrific efforts are generous by the researchers to search for a drug to control SARS-CoV-2. Methods: Here, a series of arsenical derivatives were optimized and analyzed with in silico study to search the inhibitor of RNA dependent RNA polymerase (RdRp), the major replication factor of SARS-CoV-2. All the optimized derivatives were blindly docked with RdRp of SARS-CoV-2 using iGEMDOCK v2.1. Results: Based on the lower idock score in the catalytic pocket of RdRp, darinaparsin (-82.52 kcal/mol) revealed most effective among them. Darinaparsin strongly binds with both Nsp9 replicase protein (-8.77 kcal/mol) and Nsp15 endoribonuclease (-8.3 kcal/mol) of SARS-CoV-2 as confirmed from the AutoDock analysis. During infection, the ssRNA of SARS-CoV2 is translated into large polyproteins forming viral replication complex by specific proteases like 3CL protease and papain protease. This is also another target to control the virus infection where darinaparsin also perform the inhibitory role to proteases of 3CL protease (-7.69 kcal/mol) and papain protease (-8.43 kcal/mol). Conclusion: In host cell, the furin protease serves as a gateway to the viral entry and darinaparsin docked with furin protease which revealed a strong binding affinity. Thus, screening of potential arsenic drugs would help in providing the fast invitro to in-vivo analysis towards development of therapeutics against SARS-CoV-2.

One pot synthesis, in silico study and evaluation of some novel flavonoids as potent topoisomerase II inhibitors

2021 ◽  
Vol 40 ◽  
pp. 127916
Author(s):  
Aniket P. Sarkate ◽  
Vidya S. Dofe ◽  
Shailee V. Tiwari ◽  
Deepak K. Lokwani ◽  
Kshipra S. Karnik ◽  
...  
Keyword(s):  
In Silico ◽  
Topoisomerase Ii ◽  
One Pot ◽  
Topoisomerase Ii Inhibitors ◽  
One Pot Synthesis ◽  
In Silico Study
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