scholarly journals Screening of blue crab (Portunus pelagicus L.) eyestalk extracts for growth inhibition of Pectobacterium carotovorum subsp. carotovorum (Hauber et at. 1998) (Erwinia carotovora) Jones (Holland) and Raistonia solanacearum (Yabuuchi et al. 1995) (Pseudomonas solanacearum) E. F. Smith

2006 ◽  
pp. 92-110
Author(s):  
Julissah Evangelio ◽  
Bernardita Germano

Crustacean eyestalks are known to contain neurosecretory hormones that inhibit molting, growth and gonadal development. Along this framework, this study was conducted to: screen crude eyestalk extracts from the blue crab Portunus pelagicus for possible growth inhibition on two bacterial pathogens Ralstonia solanacearum and Pectobacterium carotovorum subsp. carotovorum. and determine effective concentrations of the crude extract against the pathogens. The screening was done through bioassay using the top agar inoculation technique with 70% ethanol as the extracting solvent and streptomycin as the positive control. Different concentrations of blue crab extract and streptomycin were obtained using serial dilution.


2008 ◽  
Vol 38 (3) ◽  
pp. 583-584
Author(s):  
Bernardo A. Halfeld-Vieira ◽  
Kátia de Lima Nechet

A ocorrência de Pectobacterium carotovorum subsp. carotovorum (=Erwinia carotovora subsp. carotovora) em cebolinha (Allium fistulosum) é relatada pela primeira vez na região norte do Brasil. Até então sua ocorrência estava registrada apenas no Distrito Federal.



Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2667-2667 ◽  
Author(s):  
N. Zlatković ◽  
A. Prokić ◽  
K. Gašić ◽  
N. Kuzmanović ◽  
M. Ivanović ◽  
...  


2017 ◽  
Vol 107 (11) ◽  
pp. 1322-1330 ◽  
Author(s):  
Huan Jiang ◽  
Mengyi Jiang ◽  
Liuke Yang ◽  
Peiyan Yao ◽  
Lin Ma ◽  
...  

Pectobacterium carotovorum subsp. carotovorum strain PccS1, a bacterial pathogen causing soft rot disease of Zantedeschia elliotiana (colored calla), was investigated for virulence genes induced by the host plant. Using a promoter-trap transposon (mariner), we obtained 500 transposon mutants showing kanamycin resistance dependent on extract of Z. elliotiana. One of these mutants, PM86, exhibited attenuated virulence on both Z. elliotiana and Brassica rapa subsp. pekinensis. The growth of PM86 was also reduced in minimal medium (MM), and the reduction was restored by adding plant extract to the MM. The gene containing the insertion site was identified as rplY. The deletion mutant ΔrplY, exhibited reduced virulence, motility and plant cell wall-degrading enzyme production but not biofilm formation. Analysis of gene expression and reporter fusions revealed that the rplY gene in PccS1 is up-regulated at both the transcriptional and the translational levels in the presence of plant extract. Our results suggest that rplY is induced by Z. elliotiana extract and is crucial for virulence in P. carotovorum subsp. carotovorum.



2009 ◽  
Vol 44 (3) ◽  
pp. 327-330 ◽  
Author(s):  
Victor Rafael Barra ◽  
Reginaldo da Silva Romeiro ◽  
Flávio Augusto de Oliveira Garcia ◽  
Andréa Bittencourt Moura ◽  
Harllen Sandro Alves Silva ◽  
...  

O objetivo deste trabalho foi avaliar procariotas quanto ao potencial de antagonismo direto para o biocontrole da podridão-mole-do-tomateiro (Pectobacterium carotovorum subsp. carotovorum). Avaliaram-se 45 isolados bacterianos pelo teste de antibiose contra o patógeno. Foram feitos dois ensaios em que sementes de tomate (Lycopersicon esculentum Mill.) cv. Santa Clara foram infectadas com isolados antagônicos. As mudas foram transplantadas para solos infestados com suspensões de propágulos P. carotovorum com OD540 de 0,45 e 0,65. Os antagonistas UFV-0005, UFV-043, UFV-BF112 e UFV-0006 foram eficientes em proteger plantas de tomateiro contra a podridão-mole.





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