Application of cell biotechnologies in the treatment of gum recession

2019 ◽  
pp. 50-55 ◽  
Author(s):  
S.P. Rubnikovich ◽  
I.D. Volotovsky ◽  
Yu.L. Denisova ◽  
L.N. Dedova ◽  
V.A. Andreeva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Orthodontic Treatment ◽  
Good Condition ◽  
Gingival Recession ◽  
Long Term Results ◽  
Cell Transplant ◽  
Complex Treatment ◽  
Periodontal Tissues ◽  
Autologous Mesenchymal Stem Cells

The aim of the study is to develop and scientifically justify a new method for treating patients with gingival recession using autologous mesenchymal stem cells of adipose tissue. Objects and methods. A complex treatment was conducted in 93 patients with an anatomical recession of the gums in combination with dentoalveolar anomalies at the age of 20 to 34 years. Patients were divided into 3 groups (1st, 2nd and 3rd groups) depending on the treatment used. All patients underwent periodontological treatment. In the second group, patients included periodontological treatment including orthodontic equipment, in the third group, orthodontic treatment and the use of a cell transplant on the basis of a mixture of autologous mesenchymal stem cells and sterile bioplastic collagen material “Collost”. Results and discussion. A comprehensive assessment of the periodontal tissue of the three groups showed that among the patients who received the standard treatment complex, a good condition of the periodontal tissues 1 to 2 years after treatment was noted only in 16.7% of the patients of the 1st group. Inclusion of orthodontic treatment made it possible to obtain good results in 25% of patients in the 2nd group, which is 8% more than in the 1st group. At the same time, good results of treatment were revealed in 94% of patients in group 3, who included periodontological and orthodontic treatment using autologous mesenchymal stem cells, which confirms the need for measures aimed at normalization position of teeth, dentition and bite with the use of small orthodontic long-acting forces, improving microcirculation of periodontal tissues and restoring the contour of the marginal gum. Conclusions. Inclusion of autologous mesenchymal stem cells in the complex treatment of patients with gingival recession is accompanied by a 1.6-fold decrease in the prevalence and gum recession (1.15 mm), and tissue volume increased 2.6-fold, stimulation of periodontal microcirculation processes (in terms of capillary the periodontal pressure was 1.1 times and the intensity of periodontal microcirculation was 3.1 times), as well as the normalization of the position of teeth, dentition and occlusion, which allowed to obtain good long-term results in 94% of patients.

scholarly journals Clinical reasoning of application of stem cells in the treatment of gingival recession

2019 ◽  
Vol 63 (4) ◽  
pp. 476-484 ◽  
Author(s):  
Sergei P. Rubnikovich ◽  
Igor D. Volotovsky ◽  
Yulia L. Denisova ◽  
Vasilina A. Andreeva ◽  
Galina Yu. Panasenkova
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Orthodontic Treatment ◽  
Good Condition ◽  
Gingival Recession ◽  
Long Term Results ◽  
Cell Transplant ◽  
Complex Treatment ◽  
Periodontal Tissues ◽  
Autologous Mesenchymal Stem Cells

The aim of the study is to improve the results of complex treatment of patients with gingival recession on the basis of validation of new, highly effective methods using autologous mesenchymal stem cells. A complex treatment was conducted in 93 patients with an anatomical recession of the gum in combination with dentoalveolar anomalies at the age of 20 to 29 years. Patients were divided into 3 groups depending on the treatment used. All patients underwent periodontological treatment. In the 2nd group, patients underwent periodontological treatment including orthodontic equipment, in the 3rd group, they underwent orthodontic treatment, and a cell transplant based on a mixture of autologous mesenchymal stem cells and sterile bioplastic collagen material “Collost” was used. A comprehensive assessment of the periodontal tissue of the three groups showed that among the patients who received the standard treatment complex, a good condition of the periodontal tissues in 1 to 2 years after treatment was noted only in 16.7 % of the patients of the 1st group. Inclusion of orthodontic treatment made it possible to obtain good results in 25 % of patients in the 2nd group, which is 8 % more than in the 1st group. At the same time, good treatment results were revealed in 94 % of the 3rd group patients, who underwent periodontological and orthodontic treatment using autologous mesenchymal stem cells, which confirms the need for measures aimed to normalize the position of teeth, dentition and bite with the use of small orthodontic long-acting forces, to improve the microcirculation of periodontal tissues, and to restore the contour of marginal dreams. Inclusion of autologous mesenchymal stem cells in the complex treatment of patients with gingival recession is accompanied by a 1.6-fold decrease in the prevalence and gum recession (1.15 mm), and a 2.6-fold increase in the tissue volume, by the stimulation of periodontal microcirculation processes (the capillary periodontal pressure is increased by a factor of 1.1 time and the intensity of periodontic microcirculation is increased by a factor of 3.1 times), as well as by the normalization of the position of teeth, dentition and occlusion, which allowed one to obtain good long-term results in 94 % of patients.

scholarly journals APPLICATION OF CELLULAR TECHNOLOGIES FOR REGENERATION OF PERIODONTAL TISSUES IN EXPERIMENT

2018 ◽  
Vol 62 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Sergei P. Rubnikovich ◽  
Igor D. Volotovsky ◽  
Yulia L. Denisova ◽  
Тatiana E. Vladimirskaya ◽  
Vasilina A. Andreeva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Morphological Changes ◽  
Fibrous Tissue ◽  
Laboratory Animals ◽  
Control Group ◽  
Cell Transplant ◽  
Periodontal Tissues ◽  
White Rats

A promising scope of modern scientific research is the use of autologous and allogeneic mesenchymal stem cells for regeneration of periodontal tissues. The aim of the study was to evaluate the nature of morphological changes in the pathologically altered periodontal tissues after injection of a biotransplant containing mesenchymal stem cells of the adipose tissue immobilized on a collagen carrier in an animal experiment. In the experiment, 60 randobbred females of white rats were used as a model, whose adipose tissue was taken to obtain allogenic mesenchymal stem cells. All animals were divided into 5 groups, depending on the planned method of treatment – 10 rats each. The control group consisted of 10 laboratory animals with healthy gingiva. The experimental gingival recession model was created by the V-shaped excision of periodontal tissues. The bioplastic collagen material “Collost” gel 7 % in isolated form determines the fibrosis intensification and serves as a “matrix” for the formation of a fibrous tissue, ensures the adhesion of stem cells and their transformation into pro- and fibroblasts. Injection of a cell transplant suspension into physiological saline activates the processes of cell proliferation and transformation of fibroblast differentiating cells. Suspension of the cell transplant on a sterile bioplastic collagen material “Collost” gel 7 % enhances the effects of gel and stem cells, promotes the leveling of atrophic and dystrophic changes in the gum, strengthening a mechanical component, reducing the recession of the gum and the depth of the gingival pocket.

scholarly journals USING MESENCHYMAL STEM CELLS FOR TREATING GINGIVAL RECESSION IN THE EXPERIMENT

2018 ◽  
Vol 25 (5) ◽  
pp. 83-92
Author(s):  
S. P. Rubnikovich ◽  
Yu. L. Denisova ◽  
V. A. Andreeva ◽  
G. Yu. Panasenkova ◽  
I. S. Khomich
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Experimental Studies ◽  
Gingival Recession ◽  
Laboratory Animals ◽  
Control Group ◽  
Periodontal Tissues ◽  
White Rats ◽  
Clinical Changes ◽  
Complete Regeneration

Aim. This research was conducted to study the type of clinical changes in the diseased periodontal tissues by means of using mesenchymal stem cells for treating gingival recession in the experiment.Materials and methods. Adipose tissue in a volume of 1-2 ml was sampled from one specimen in sterile conditions in order to obtain allogeneic mesenchymal stem cells before the beginning of the experimental studies. Random-bred female white rats (60 specimens) were used as a model in the experiment. The surgical intervention was performed in the area of incisors on the upper and lower jaws of rats. All animals were divided into 5 groups (10 rats in each group) depending on the planned method of the treatment. The control group, intact rats, consisted of 10 laboratory animals with a healthy gingiva. The creation of a model of the experimental gingival recession was carried out by the mechanical V-shaped excision of periodontal tissues.Results. The experimental studies showed the possibility of complete regeneration of the dentogingival papillae as well as periodontal tissues regeneration in the area of the experimental gingival recession during the first 28 days. Meanwhile, there is a complete absence of inflammatory signs in the 5th group (hyperemia, edema, gingival bleeding), along with their significant decrease in the 3rd and the 4th groups that confirms the pronounced therapeutic effect of mesenchymal stem cells and sterile bioplastic collagen material «Collost» (7% gel).The conclusion. While studying the type of clinical changes in diseased periodontal tissues using mesenchymal stem cells in the treatment of gingival recession, we established the positive dynamics in the experiment. The decrease in the intensity and prevalence of gingival inflammation starts from the 14th day. On the 28th day there was noted a complete absence of inflammatory signs in the animals after injecting the transplant consisting of the cell suspension on the sterile bioplastic material Collost (7% gel). Furthermore, the significant increase in the regeneration of the gingival margin was observed on the 24th day from the moment of creating the gingival recession model. The complete regeneration of the dentogingival papillae and the absence of the gingival recession were noted on the 38th day of general observation. 

Changes of Periodontal Tissues Because of Orthodontic Treatment: A Retrospective Study

2021 ◽  
pp. 232020682110034
Author(s):  
Hamad Alzoman ◽  
Khalid Alamoud ◽  
Waad K. Alomran ◽  
Abdullazez Almudhi ◽  
Naif A. Bindayel
Keyword(s):  
Retrospective Study ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Positive Impact ◽  
Demographic Data ◽  
Gingival Recession ◽  
Confounding Factors ◽  
Crown Height ◽  
Periodontal Tissues ◽  
Before And After

Aim: To evaluate the periodontal status before and after orthodontic treatment and to analyze the confounding factors associated with it. Materials and Methods: The present retrospective study analyzed pre- and posttreatment records of a total of 60 patients. Intraoral digital photographs before and after the orthodontic treatment were used to measure the following three periodontal variables: (a) width of the keratinized gingiva, (b) gingival recession, and (c) the status of interdental papilla. The ImageJ 46 software imaging program was used to perform the required measurements. The clinical data were then analyzed in an association with the participants’ demographic data, the type of orthodontic tooth movement, and other confounding factors. The data obtained were manually entered into the statistical package and analyzed using a significance level set at P < .05. Descriptive statistics, paired t-tests, and Pearson’s correlation analysis were used to identify differences among the studied variables. Results: Among the 60 patients, the majority (66.7%) were females. Most cases of the treated malocclusion were Class II, and most patients underwent intrusion and retrusion movements. The width of keratinized gingiva increased for most sites of upper teeth. In contrast, the lower teeth showed a mix of improvement and compromised width of keratinized gingiva. As a measure of gingival recession, the difference of clinical crown height revealed the tendency toward a reduced clinical crown height for most sites. Upper left lateral incisors and canines showed significant values with regard to keratinized gingival width measurements and tooth movements such as extrusion and intrusion movements. Likewise, with regard to the clinical crown height, upper right central incisors showed significant differences when correlated with the interincisal angle, lower incisors to the mandibular plane, and upper incisors to the NA line. The upper lateral incisors also showed significant correlations to certain cephalometric measurements. Conclusions: The orthodontic treatment was found to exert a significantly positive impact on the surrounding periodontium, particularly in the upper canine areas. Likewise, various types of tooth movement were found to positively affect the periodontium.

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