scholarly journals ATP-binding Cassette (ABC) Transporter Proteins in Highly Terbinafine-resistant Strains of Trichophyton indotineae (Former Species Name: Trichophyton interdigitale)

2021 ◽  
Vol 62 (1) ◽  
pp. 21-25
Author(s):  
Rui Kano
Keyword(s):  
Abc Transporter ◽  
Atp Binding ◽  
Transporter Proteins ◽  
Trichophyton Interdigitale ◽  
Resistant Strains ◽  
Atp Binding Cassette

scholarly journals Contributions of Aspergillus fumigatus ATP-Binding Cassette Transporter Proteins to Drug Resistance and Virulence

2013 ◽  
Vol 12 (12) ◽  
pp. 1619-1628 ◽  
Author(s):  
Sanjoy Paul ◽  
Daniel Diekema ◽  
W. Scott Moye-Rowley
Keyword(s):  
Drug Resistance ◽  
Aspergillus Fumigatus ◽  
Abc Transporter ◽  
Abc Transporters ◽  
Sequence Similarity ◽  
Atp Binding ◽  
Content Type ◽  
Transporter Proteins ◽  
Encoding Genes ◽  
Atp Binding Cassette

ABSTRACTIn yeast cells such as those ofSaccharomyces cerevisiae, expression of ATP-binding cassette (ABC) transporter proteins has been found to be increased and correlates with a concomitant elevation in azole drug resistance. In this study, we investigated the roles of twoAspergillus fumigatusproteins that share high sequence similarity withS. cerevisiaePdr5, an ABC transporter protein that is commonly overproduced in azole-resistant isolates in this yeast. The twoA. fumigatusgenes encoding the ABC transporters sharing the highest sequence similarity toS. cerevisiaePdr5 are calledabcAandabcBhere. We constructed deletion alleles of these two different ABC transporter-encoding genes in three different strains ofA. fumigatus. Loss ofabcBinvariably elicited increased azole susceptibility, whileabcAdisruption alleles had variable phenotypes. Specific antibodies were raised to both AbcA and AbcB proteins. These antisera allowed detection of AbcB in wild-type cells, while AbcA could be visualized only when overproduced from thehspApromoter inA. fumigatus. Overproduction of AbcA also yielded increased azole resistance. Green fluorescent protein fusions were used to provide evidence that both AbcA and AbcB are localized to the plasma membrane inA. fumigatus. Promoter fusions to firefly luciferase suggested that expression of both ABC transporter-encoding genes is inducible by azole challenge. Virulence assays implicated AbcB as a possible factor required for normal pathogenesis. This work provides important new insights into the physiological roles of ABC transporters in this major fungal pathogen.

scholarly journals The human ATP-binding cassette (ABC) transporter superfamily

2001 ◽  
Vol 42 (7) ◽  
pp. 1007-1017 ◽  
Author(s):  
Michael Dean ◽  
Yannick Hamon ◽  
Giovanna Chimini
Keyword(s):  
Abc Transporter ◽  
Atp Binding ◽  
Atp Binding Cassette

scholarly journals Nucleotide-dependent Allostery within the ABC Transporter ATP-binding Cassette

2007 ◽  
Vol 282 (31) ◽  
pp. 22793-22803 ◽  
Author(s):  
Peter M. Jones ◽  
Anthony M. George
Keyword(s):  
Abc Transporter ◽  
Atp Binding ◽  
Atp Binding Cassette

scholarly journals ATP Binding Cassette Transporter A1 is Involved in Extracellular Secretion of Acetylated APE1/Ref-1

2019 ◽  
Vol 20 (13) ◽  
pp. 3178 ◽  
Author(s):  
Yu Ran Lee ◽  
Hee Kyoung Joo ◽  
Eun Ok Lee ◽  
Hyun Sil Cho ◽  
Sunga Choi ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Abc Transporters ◽  
Secretory Pathway ◽  
Trichostatin A ◽  
Atp Binding ◽  
Terminal Protein ◽  
Secretion Signal ◽  
Extracellular Secretion ◽  
Atp Binding Cassette Transporter ◽  
Atp Binding Cassette

Acetylation of nuclear apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE1/Ref-1) is associated with its extracellular secretion, despite the lack of an N-terminal protein secretion signal. In this study, we investigated plasma membrane targeting and translocation of APE1/Ref-1 in HEK293T cells with enhanced acetylation. While APE1/Ref-1 targeting was not affected by inhibition of the endoplasmic reticulum/Golgi-dependent secretion, its secretion was reduced by inhibitors of ATP-binding cassette (ABC) transporters, and siRNA-mediated down-regulation of ABC transporter A1. The association between APE1/Ref-1 and ABCA1 transporter was confirmed by proximal ligation assay and immunoprecipitation experiments. An APE1/Ref-1 construct with mutated acetylation sites (K6/K7R) showed reduced co-localization with ABC transporter A1. Exposure of trichostatin A (TSA) induced the acetylation of APE1/Ref-1, which translocated into membrane fraction. Taken together, acetylation of APE1/Ref-1 is considered to be necessary for its extracellular targeting via non-classical secretory pathway using the ABCA1 transporter.

scholarly journals Cystathionine β-Synthase (CBS) Domains 1 and 2 Fulfill Different Roles in Ionic Strength Sensing of the ATP-binding Cassette (ABC) Transporter OpuA

2011 ◽  
Vol 286 (43) ◽  
pp. 37280-37291 ◽  
Author(s):  
Akira Karasawa ◽  
Guus B. Erkens ◽  
Ronnie P.-A. Berntsson ◽  
Renee Otten ◽  
Gea K. Schuurman-Wolters ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Abc Transporter ◽  
Atp Binding ◽  
Cbs Domains ◽  
Atp Binding Cassette

scholarly journals Mutations that alter the transmembrane signalling pathway in an ATP binding cassette (ABC) transporter.

1994 ◽  
Vol 13 (7) ◽  
pp. 1752-1759 ◽  
Author(s):  
K.M. Covitz ◽  
C.H. Panagiotidis ◽  
L.I. Hor ◽  
M. Reyes ◽  
N.A. Treptow ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Signalling Pathway ◽  
Atp Binding ◽  
Transmembrane Signalling ◽  
Atp Binding Cassette
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