scholarly journals Review on Plants Fingerprinting and Tlc Fingerprinting of Two Aqueous Herbal Products

2018 ◽  
Vol 3 (3) ◽  
Keyword(s):  
Systematic Review ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chemical Method ◽  
Herbal Medicines ◽  
Common Type ◽  
Herbal Products ◽  
Herbal Preparations ◽  
Layer Chromatography

As herbal medicines have become commercialized, the safety, quality and efficacy of medicinal plants and herbal products have become of great concern. In view of this a review on Plant fingerprinting was carried out using systematic review and TLC fingerprinting was also carried out on two herbal products using simple Thin Layer Chromatography. The results revealed Morphological, Chemical and genetic methods are the major types studied. The High Performance Thin Layer Chromatography (HPTLC) is the most common type to chemical method utilized and most of the studies on Plants fingerprinting were carried out in Asia. Comparing the TLC profile of the two herbal preparations reveals that the two products are basically the same

scholarly journals What’s in the box? Authentication ofEchinaceaherbal products using DNA metabarcoding and HPTLC

2017 ◽  
Author(s):  
Ancuta Cristina Raclariu ◽  
Carmen Elena Ţebrencu ◽  
Mihael Cristi Ichim ◽  
Oana Teodora Ciupercǎ ◽  
Anne Krag Brysting ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
The United States ◽  
Infrared Spectrometry ◽  
Herbal Products ◽  
Analytical Strategy ◽  
Regulatory Policies ◽  
Dna Metabarcoding ◽  
Layer Chromatography

AbstractBackgroundDifferences in regulatory policies between countries as well as a lack of appropriate standardized methods for authentication and quality control of herbal products lead to concerns over quality and safety.Echinaceaproducts are among the top selling herbal products in Europe and the United States with indications for broad range of ailments.PurposeThis study approached the need for a novel analytical strategy in authentication of herbal products.MethodsA combination of high performance thin layer chromatography (HPTLC) and DNA metabarcoding was employed. Fifty-threeEchinaceaherbal products marketed across Europe were tested to evaluate the accuracy of these methods in plant identification and their potential in detecting substitutes, adulterants and other unreported plant constituents.ResultsHPTLC provides a high resolution in detectingEchinaceaphytochemical target compounds, but does not offer information on the other species within the product. Alternatively, we showed that the limitation of HPTLC to detect non-targeted species can be overcome through the complementary use of DNA metabarcoding. Using DNA metabarcoding,Echinaceaspecies were detected in 34 out of the 38 retained products (89 %), but with a lack of discriminatory resolution at the species level due to the low level of molecular divergence within theEchinaceagenus. All herbal products showed considerable discrepancies between ingredients listed on the label and the ones detected using DNA metabarcoding registering an overall ingredient fidelity of 43 %.ConclusionThe results confirm that DNA metabarcoding can be used to test for the presence ofEchinaceaand simultaneously to detect other species present in even highly processed and multi-ingredient herbal products.AbbreviationsEMAEuropean Medicines AgencyBLASTbasic local alignment search toolFTIRFourier-transformed infrared spectroscopyIRinfrared spectrometryHPTLChigh performance thin layer chromatographymatKmaturase KMSmass spectrometryMOTUmolecular taxonomic unitnrITSnuclear ribosomal internal transcribed spacerPh.Eur.European PharmacopoeiarbcLribulose bisphosphate carboxylaseTLCthin-layer chromatographyUV-VISultraviolet-visible

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