scholarly journals Development of Specific Nano-Antibody for Application in Selective and Rapid Environmental Diagnoses of Salmonella arizonae

2020 ◽  
Vol 10 (6) ◽  
pp. 7198-7208
Keyword(s):  
Sandwich Elisa ◽  
Economic Value ◽  
Bacterial Strains ◽  
Western Blot Assay ◽  
Phage Display Technique ◽  
Sds Page ◽  
Diagnostic Agents ◽  
Pcr Products ◽  
Dna Fragment ◽  
Display Technique

Treatment of human and animals for protection from pathogens infection has significant economic value, especially with the harmful Salmonella arizonae. Beneficial cameloid heavy-chain antibodies as single-domain antigen-binding fragments known as VHHs or nano-bodies may be the acceptable option for producing the treatment and/or diagnostic agents. In the current study, we developed a sandwich ELISA based nano-body towards S. arizonae as the first report for the treatment of S. arizonae. using the cDNA synthesized from immunized camels RNA to isolate 700 bp DNA fragment, which contains all VH domains of IgG2 and IgG3 isotypes followed by the second amplification VHH PCR with amplified fragments at 450 bp. The final PCR products were cloned into the phagemid vector pMECS then via phage display technique. Nano-antibodies protein was purified and separated under non-denaturing conditions by SDS-PAGE. The reactivity of each VHH of the selected clones was analyzed by Western blot assay. The isolated nano-antibodies showed binding not only to S. arizonae, but also to other bacterial strains, indicating that these nano-antibodies can be used in treatment but cannot use in diagnostic.

scholarly journals Expression, purification and characterization of anti-FGF7 domain antibody identified using phage display technique

2021 ◽  
Author(s):  
Aliakbar Alizadeh ◽  
Mona Roshani ◽  
Omid Jamshidi Kandjani ◽  
Milad Soltani-Saif ◽  
Siavoush Dastmalchi
Keyword(s):  
Phage Display ◽  
Md Simulations ◽  
Docking Study ◽  
Single Domain Antibody ◽  
Cancer Cell Growth ◽  
Phage Display Technique ◽  
Domain Antibody ◽  
Sds Page ◽  
Display Technique

Background: Fibroblast growth factors (FGFs) are involved in angiogenesis, wound healing and embryonic development. However, one of the causes of cancer cell growth in fibroblast-dependent cancers is FGF7 secreted by fibroblasts. Therefore, antibodies against FGF7 can be used for treatment of these types of cancers. Methods: In previous studies, a phage displaying single domain antibody, D53, against human FGF7 has been identified using the phage display technique. In the present study, D53 was produced and purified in its isolated form. ELISA experiment was performed to evaluate the binding of D53 to FGF7. The mode of interaction of D53-FGF7 was explored using docking study and molecular dynamics (MD) simulations. Results: The expression and purification processes were verified using western blotting and SDS-PAGE analyses. ELISA experiment showed that D53 is able to recognize and bind FGF7. Docking study and MD simulations indicated that compared to dummy VH, D53 has more affinity towards FGF7. Conclusion: The findings in the current study can be useful for generation and development of FGF7 inhibitors with potential use in fibroblast-dependent cancers.

Development of Human Antibody Inhibiting RNase H Activity of Polymerase of Hepatitis B Virus Using Phage Display Technique

2004 ◽  
Vol 4 (1) ◽  
pp. 16
Author(s):  
Seong-Rak Lee ◽  
Eun-Kyoung Song ◽  
Young-Joo Jeong ◽  
Young-Yi Lee ◽  
Ik-Jung Kim ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Phage Display ◽  
Rnase H ◽  
Human Antibody ◽  
Phage Display Technique ◽  
B Virus ◽  
Display Technique

scholarly journals Antibacterial and Immunomodulatory Potentials of Biosynthesized Ag, Au, Ag-Au Bimetallic Alloy Nanoparticles Using the Asparagus racemosus Root Extract

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2453
Author(s):  
Musarat Amina ◽  
Nawal M. Al Musayeib ◽  
Nawal A. Alarfaj ◽  
Maha F. El-Tohamy ◽  
Gadah A. Al-Hamoud
Keyword(s):  
Noble Metals ◽  
Solid Phase ◽  
Sandwich Elisa ◽  
Root Extract ◽  
Asparagus Racemosus ◽  
Alloy Nanoparticles ◽  
Bacterial Strains ◽  
Microwave Assisted ◽  
Bimetallic Alloy ◽  
Cytokine Levels

Two noble metals, such as silver and gold alloy nanoparticles, were successfully synthesized by the microwave assisted method in the presence of the Asparagus racemosus root extract and were used as an antibacterial and immunomodulatory agent. The nanostuctures of the synthesized nanoparticles were confirmed by various spectroscopic and microscopic techniques. The UV-vis spectrum exhibits a distinct absorption peak at 483 nm for the bimetallic alloy nanoparticles. The microscopic analysis revealed the spherical shaped morphology of the biosynthesized nanoparticles with a particle size of 10–50 nm. The antibacterial potential of the green synthesized single metal (AgNPs and AuNPs) and bimetallic alloy nanoparticles was tested against five bacterial strains. The bimetallic alloy nanoparticles displayed the highest zone of inhibition against P. aeurgnosia and S.aureus strains when compared to single metal nanoparticles and plant extract. In addition, the inmmunomodulatory potential of the root extract of A. racemosus, AgNPs, AuNPs, and Ag-Au alloy NPs is achieved by measuring the cytokine levels in macrophages (IL-1β, IL-6, and TNF-α) and NK cells (IFN-γ) of NK92 and THP1 cells using the solid phase sandwich ELISA technique. The results showed that the root extract of A. racemosus, AgNPs, and AuNPs can reduce the pro-inflammatory cytokine levels in the macrophages cells, while Ag-Au alloy NPs can reduce cytokine responses in NK92 cells. Overall, this study shows that the microwave assisted biogenic synthesized bimetallic nanoalloy nanoparticles could be further explored for the development of antibacterial and anti-inflammatory therapies.

Antibacterial Activities of Antibacterial Proteins/Peptides Isolated from Organs and Mucus of Clarias Gariepinus Reared at High Stocking Density

2012 ◽  
Vol 455-456 ◽  
pp. 455-460 ◽  
Author(s):  
Xiao Mei Wang ◽  
Wei Dai ◽  
Ke Zhing Xing ◽  
Tian Jun Li ◽  
Xin Wang
Keyword(s):  
Molecular Weight ◽  
Antibacterial Activity ◽  
Crude Protein ◽  
Clarias Gariepinus ◽  
Gel Filtration ◽  
Stocking Density ◽  
Antibacterial Activities ◽  
Edwardsiella Tarda ◽  
Bacterial Strains ◽  
Sds Page

. Antibacterial proteins/peptides are important parts of the innate immune system in Clarias gariepinus. To examine potential antibacterial proteins/peptides in organs and mucus of C. gariepinus, crude protein/peptide extracts were isolated with ammonium sulfate precipitation from mucus, skin, gill, suprabranchial organ and intestine. Following further extraction using Sephadex G-50 gel filtration chromatography, the proteins/peptides associated with two absorption peaks (AP1 and AP2) were pooled, respectively, and assayed for their antibacterial activities against Escherichia coli, Aeromonas hydrophila and Edwardsiella tarda. The results showed that AP1 and AP2 from all the sampled tissues and mucus at concentration of 100 mg mL-1 exhibited antibacterial activity against the tested bacterial strains. Differences in antibacterial activity were observed among sample extracts. The protein profiles of AP1 obtained by Tricine-SDS-PAGE gel showed a broad range of peptides/proteins, and molecular weight of the mutual abundant peptide obtained was about 27 kDa. Antibacterial activity of AP2 extracted from intestine was due to peptide with molecular weight of 5.5 kDa.

Phage-based Nanomedicines as New Immune Therapeutic Agents for Breast Cancer

2018 ◽  
Vol 24 (11) ◽  
pp. 1195-1203 ◽  
Author(s):  
Atefeh Arab ◽  
Rezvan Yazdian Robati ◽  
Jessica Nicastro ◽  
Roderick Slavcev ◽  
Javad Behravan
Keyword(s):  
Breast Cancer ◽  
Phage Display ◽  
Biomedical Applications ◽  
Therapeutic Agents ◽  
Molecular Method ◽  
Specific Antibodies ◽  
Phage Display Technique ◽  
Specific Antigens ◽  
Protein Libraries ◽  
Display Technique

Despite years of investigation, breast cancer remains a major cause of death worldwide. Phage display is a powerful molecular method in which peptide and protein libraries can be displayed via genetic fusions on the surface of phages. This approach has tremendous potential for biomedical applications and has already facilitated the discovery of specific antibodies, specific antigens, and peptides with potential roles in the diagnosis and treatment of malignancies including breast cancer. In this review, we discuss the new and the latest advancements in the applications of the phage display technique in the provision of immune therapeutics for breast cancer.

scholarly journals Use of a Phage Display Technique to Identify Potential Osteoblast Binding Sites Within Osteoclast Lacunae

2002 ◽  
Vol 17 (5) ◽  
pp. 915-922 ◽  
Author(s):  
Tzong-Jen Sheu ◽  
Edward M. Schwarz ◽  
Regis J. O'keefe ◽  
Randy N. Rosier ◽  
J. Edward Puzas
Keyword(s):  
Phage Display ◽  
Binding Sites ◽  
Phage Display Technique ◽  
Display Technique
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