scholarly journals Spectroscopic Fourier Transform Infrared Study of Poly (N-Isopropylacrylamide)-Human Recombinant Platelet-Derived Growth Factor- BB Protein as a Strategic Biobetter Regenerative Material

2021 ◽  
Vol 11 (3) ◽  
pp. 3927-3933
Keyword(s):  
Fourier Transform ◽  
Growth Factor ◽  
Fourier Transform Infrared ◽  
Platelet Derived Growth Factor ◽  
Thermoresponsive Polymer ◽  
Infrared Study ◽  
Biophysical Approach ◽  
Aggregation Tendency ◽  
Better Than

Biobetter or Biosuperior are recombinant protein drugs with one or more characteristics that are better than the original formulation. Platelet-Derived Growth Factor (PDGF) is a potent mitogen that can induce pulp stem cell proliferation and promote angiogenesis in vitro. PDGF-BB is required for wound healing, and they are chemoattractants that can activate macrophages and fibroblast activation in vitro. Poly(n-isopropylacrylamide) (PNIPAM) polymer is a thermoresponsive polymer widely used for drug delivery. A lyophilized combination of PNIPAM and 5µg of Recombinant Human PDGF-BB(rhPDGF-BB)(sigma Aldrich-P3201) protein was prepared in the present study. This combination was subjected to Spectroscopic Fourier-Transform Infrared(FTIR) study. The results suggested that a biophysical approach like FTIR enables assessing protein stability and aggregation tendency of recombinant proteins, contributing as a useful method to identify samples with prospective high therapeutic values.

scholarly journals Variety Identification of Orchids Using Fourier Transform Infrared Spectroscopy Combined with Stacked Sparse Auto-Encoder

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2506 ◽  
Author(s):  
Yunfeng Chen ◽  
Yue Chen ◽  
Xuping Feng ◽  
Xufeng Yang ◽  
Jinnuo Zhang ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Principal Component ◽  
Fourier Transform Infrared ◽  
Spectroscopic Technique ◽  
Variety Identification ◽  
Support Vector ◽  
K Nearest Neighbors ◽  
Discriminant Models ◽  
The Fourier Transform ◽  
Better Than

The feasibility of using the fourier transform infrared (FTIR) spectroscopic technique with a stacked sparse auto-encoder (SSAE) to identify orchid varieties was studied. Spectral data of 13 orchids varieties covering the spectral range of 4000–550 cm−1 were acquired to establish discriminant models and to select optimal spectral variables. K nearest neighbors (KNN), support vector machine (SVM), and SSAE models were built using full spectra. The SSAE model performed better than the KNN and SVM models and obtained a classification accuracy 99.4% in the calibration set and 97.9% in the prediction set. Then, three algorithms, principal component analysis loading (PCA-loading), competitive adaptive reweighted sampling (CARS), and stacked sparse auto-encoder guided backward (SSAE-GB), were used to select 39, 300, and 38 optimal wavenumbers, respectively. The KNN and SVM models were built based on optimal wavenumbers. Most of the optimal wavenumbers-based models performed slightly better than the all wavenumbers-based models. The performance of the SSAE-GB was better than the other two from the perspective of the accuracy of the discriminant models and the number of optimal wavenumbers. The results of this study showed that the FTIR spectroscopic technique combined with the SSAE algorithm could be adopted in the identification of the orchid varieties.

Basis of hematopoietic defects in platelet-derived growth factor (PDGF)-B and PDGF β-receptor null mice

Blood ◽  
2001 ◽  
Vol 97 (7) ◽  
pp. 1990-1998 ◽  
Author(s):  
Wolfgang E. Kaminski ◽  
Per Lindahl ◽  
Nancy L. Lin ◽  
Virginia C. Broudy ◽  
Jeffrey R. Crosby ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fetal Liver ◽  
Liver Cells ◽  
Platelet Derived Growth Factor ◽  
Wild Type ◽  
Liver Growth ◽  
Hematologic Disorders ◽  
Fetal Liver Cells ◽  
Β Receptor

Abstract Platelet-derived growth factor (PDGF)-B and PDGF β-receptor (PDGFRβ) deficiency in mice is embryonic lethal and results in cardiovascular, renal, placental, and hematologic disorders. The hematologic disorders are described, and a correlation with hepatic hypocellularity is demonstrated. To explore possible causes, the colony-forming activity of fetal liver cells in vitro was assessed, and hematopoietic chimeras were demonstrated by the transplantation of mutant fetal liver cells into lethally irradiated recipients. It was found that mutant colony formation is equivalent to that of wild-type controls. Hematopoietic chimeras reconstituted with PDGF-B−/−, PDGFRβ−/−, or wild-type fetal liver cells show complete engraftment (greater than 98%) with donor granulocytes, monocytes, B cells, and T cells and display none of the cardiovascular or hematologic abnormalities seen in mutants. In mouse embryos, PDGF-B is expressed by vascular endothelial cells and megakaryocytes. After birth, expression is seen in macrophages and neurons. This study demonstrates that hematopoietic PDGF-B or PDGFRβ expression is not required for hematopoiesis or integrity of the cardiovascular system. It is argued that metabolic stress arising from mutant defects in the placenta, heart, or blood vessels may lead to impaired liver growth and decreased production of blood cells. The chimera models in this study will serve as valuable tools to test the role of PDGF in inflammatory and immune responses.

Fourier transform infrared study of high-purity maceral types

Fuel ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 536-542 ◽  
Author(s):  
Gary R. Dyrkacz ◽  
Carol A.A. Bloomquist ◽  
Peter R. Solomon
Keyword(s):  
Fourier Transform ◽  
High Purity ◽  
Fourier Transform Infrared ◽  
Infrared Study
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