scholarly journals In vitro growth and development of Dendrobium hybrid orchid

1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Ms Medium ◽  
Fresh Weight ◽  
Maximum Weight ◽  
Shoot Height ◽  
Single Shoot ◽  
Number Of Leaves ◽  
Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

scholarly journals In-Vitro Seed Germination and Effect of Growth Regulators on Subsequent Development of Protocorms of Eulophia Nuda Lindl

2015 ◽  
Vol 3 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Varsha Dawande ◽  
Rajaram Gurav
Keyword(s):  
Seed Germination ◽  
Root Growth ◽  
Growth Regulators ◽  
Subsequent Development ◽  
Ms Medium ◽  
Asymbiotic Seed Germination ◽  
Endangered Orchid ◽  
Maximum Root ◽  
Regenerated Plantlets

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476   

scholarly journals 606 In Vitro Propagation of Some Strawberry Cultivars in Response to Kind and Concentration of Some Cytokinin Compounds

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 501D-501
Author(s):  
M.H. Aboul-Nasr ◽  
S.Z. Elagamy ◽  
A.M. Kassim
Keyword(s):  
In Vitro Propagation ◽  
Callus Formation ◽  
Shoot Length ◽  
Control Treatment ◽  
Fresh Weight ◽  
Shoot Height ◽  
Murashige And Skoog Medium ◽  
Number Of Leaves ◽  
Strawberry Cultivars

Three strawberry cultivars were used in this study. Runners from the three cultivars were grown on Murashige and Skoog medium supplemented with benzylaminpurine or kinetin at four concentrations (2, 1, 0.5, and 0.25 mg·L-1 in addition to the control treatment). The use of cytokinin, regardless of type and concentration, promoted the explant proliferation, shoot height, number of leaves, and plantlet fresh weight. Callus formation was enhanced by BA application, especially with `Pajaro'. However, `Chandler' did not form at all using cytokinin. Produced plantlets had lower nutrient constituents (N, P, K, Ca, and Na) when grown on media containing cytokinin compared to the control. Based on these results, cytokinin is recommended to get more shoots per explant. Furthermore, using the lowest concentration (0.25 mg·L-1) will produce height proliferation, greater shoot length, and more leaves.

scholarly journals In Vitro Regeneration Of Brinjal (Solanum Melongena L.)

1970 ◽  
Vol 36 (3) ◽  
pp. 397-406 ◽  
Author(s):  
BP Ray ◽  
L Hassan ◽  
KM Nasiruddin
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Callus Formation ◽  
Solanum Melongena ◽  
Ms Medium ◽  
Fresh Weight ◽  
Normal Environment ◽  
Regenerated Plantlets

The effect of different explants and concentrations of BAP and NAA on induction of callus and plant regeneration of brinjal cv. Jhumki were investigated. The treatment combinations were BAP (0. 2.0. 3.0, and 4.0 mg/l) and NAA (0. 0.1, 0.5, and 1.0 mg/l). The rate of callus formation varied in different treatments. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem, and 8.2 days required for callus induction. The highest fresh weight of callus was 1.12g from stem and 0.48g from root. The number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days. All regenerated plantlets survived in normal environment. Keywords: NAA; BAP; regeneration; brinjal. DOI: http://dx.doi.org/10.3329/bjar.v36i3.9268 BJAR 2011; 36(3): 397-406

scholarly journals Bulblet regeneration from ex vitro root explant in lily hybrids

2008 ◽  
Vol 35 (No. 3) ◽  
pp. 107-112 ◽  
Author(s):  
R. Kapoor ◽  
S. Kumar ◽  
K. Kanwar J
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Middle Zone ◽  
Ex Vitro ◽  
Fresh Weight ◽  
Root Segments ◽  
Root Explant

The influence of growth regulators on <i>in vitro</i> bulblet formation from <i>ex vitro</i> roots was studied in asiatic and oriental hybrids of <i>Lilium</i> The root segments (3–4 mm) isolated from the middle zone of 2–3 cm <i>ex vitro</i> root were cultured on Murashige and Skoog (MS) medium containing 1 or 1.5 mg/dm<sup>3</sup> naphthalene acetic acid (NAA) and/or benzyladenine (BA). Bulblets were not produced in the presence of NAA and BA alone. A significant increase in the per cent explants producing bulblets was observed with 1.5 mg/dm<sup>3</sup> NAA and 1 mg/dm<sup>3</sup> BA. Maximum number of bulblets and average fresh weight per bulblet was observed with 2 mg/dm<sup>3</sup> NAA and 1.5 mg/dm<sup>3</sup> BA after 90 days of culture. No differences were found among cultivars in bulblet regeneration of explant or bulblet number although more weighty bulblets occurred in cv. Apeldoorn. About 82% bulblet survival was recorded in coco peat after 30 days of transfer to pots.

scholarly journals In vitro clonal propagation and efficient acclimatization with use of hydrogel of intensively sweet medicinal plant Lippia dulcis Trev.

2020 ◽  
Vol 66 (4) ◽  
pp. 25-31
Author(s):  
Magdalena Tomaszewska-Sowa
Keyword(s):  
Leaf Area ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Shoot Length ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation ◽  
Number Of Leaves ◽  
In Vitro Clonal Propagation

Summary Introduction: The leaves of Lippia dulcis contain high amounts of hernandulcin. It is one thousand fold sweeter than sucrose, however, it hardly contains any calories. Objective: The aim of this research was to optimalisation of micropropagation and acclimatization of L dulcis Methods: The nodal explants were inoculated on phytohormone-free MS medium. After 6 weeks the explants were inoculated onto the MS medium with different plant growth regulators. Well-developed rooted plantlets were adapted to ex vitro conditions using hydrogel. Results: On the medium with BAP and NAA the highest number of shoots were produced. The higest average shoot length, number of the leaves and the leaf area were recorded on the medium with GA3. Adding IBA increased the number of roots. The addition of hydrogel enhanced the acclimatization efficiency. Conclusions: There was observed a positive, stimulating influence of growth regulators on mass propagation and increase in the number of leaves and the leaf area and influence of hydrogel on the development of plantlets during acclimatization.

scholarly journals In vitro regeneration potentiality of Brassica genotypes in differential growth regulators

1970 ◽  
Vol 35 (2) ◽  
pp. 189-199 ◽  
Author(s):  
MMA Khan ◽  
ABM Arif Hasan Khan Robin ◽  
MAN Nazim-Ud-Dowla ◽  
SK Talukder ◽  
L Hassan
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Root Formation ◽  
Ms Medium ◽  
Differential Growth ◽  
The Media ◽  
Regenerated Plantlets

Petiole of six genotypes of oilseed Brassica viz. Tori-7, Sampad, Kallyania, BARI Sarisha-7, BARI Sarisha-8, and MM 20-3 were cultured in MS medium with different concentrations of BAP, NAA, and AgNO3 for callus induction and subsequent plant regeneration. The highest percentage of callus induction (91.43%) was observed in Tori-7 in the media supplemented with 2 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO3. Calli were maintained in order to get sufficient number of regenerants. With the increased concentration of BAP, the highest percentage (57.14) of regenerants were found in Tori-7 followed by Sampad (33.13%) and BARI Sarisha-8 (31.42%) in MS media supplemented with 2.5 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO3. Root formation from the regenerants was found best in half MS medium supplemented with 0.5 mg/L NAA in genotype Tori-7. Regenerated plantlets of four genotypes (Tori-7, BARI Sarisha-8, Kallyania, BARI Sarisha-7) were successfully established in the field.Keywords: AgNO3; BAP; Brassica; NAA; regeneration.  DOI: 10.3329/bjar.v35i2.5881Bangladesh J. Agril. Res. 35(2) : 189-199, June 2010

scholarly journals Effects of Growth Regulators and Type-Variety of Oil Palm (Elaeis guineensis Jacq.) on Direct Organogenesis

2018 ◽  
Vol 10 (2) ◽  
pp. 251-258
Author(s):  
Chibuzo Nwabueze Chikezie NWAOGUALA ◽  
Hakeem Olalekan SHITTU
Keyword(s):  
Growth Regulators ◽  
Oil Palm ◽  
Root Length ◽  
Elaeis Guineensis ◽  
Root Production ◽  
Ms Medium ◽  
Number Of Leaves ◽  
Leaves And Roots ◽  
Elaeis Guineensis Jacq

The in vitro organogenesis of embryos of type-variety (‘Albescens’, ‘Nigrescens’, ‘Virescens’ and ‘Idolatrica’) of the oil palm (Elaeis guineensis Jacq.) with growth regulators was studied. Murashige and Skoog (MS) medium, supplemented with nine treatment concentrations of Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP), alone and in combinations, was used to initiate embryo cultures of the type-variety. Data were collected on time of root production, root length, plant height, number of leaves and number of roots.  The results obtained indicated that there were swelling and expansion of the matured zygotic explants in cultures within 7 and 14 days after culture initiation (DCI). Time of root production did not vary significantly among type-variety, but differed significantly (p < 0.001) due to growth regulators, whereas their interaction effect was non-significant. Root length and plantlet height significantly varied (p < 0.05) due to type-variety and growth regulators, as well as their interaction. There were no significant variations among type-variety on number of leaves and roots produced at 84 DCI, but there was significant (p < 0.05) variations on number of leaves and non-significant variance on number of roots due to growth regulators. The type-variety combined with growth regulators effects on number of leaves and roots were not significant.  In the present study, the responses of the various type-variety in the regeneration of plantlets in vitro varied with respect to media supplementation and that low concentration of NAA and BAP, alone and in different combinations, favoured root, leaf and plantlet production in MS medium.

scholarly journals RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO

2016 ◽  
Vol 29 (1) ◽  
pp. 18-24 ◽  
Author(s):  
RONAN CARLOS COLOMBO ◽  
VANESSA FAVETTA ◽  
RICARDO TADEU DE FARIA ◽  
FELIPE ARANHA DE ANDRADE ◽  
VANDERLI MARINO MELEM
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Average Length ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Shoot Height ◽  
Leaf Tissues ◽  
Number Of Leaves ◽  
Scanning Electron

ABSTRACT: Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii.

scholarly journals Impact of Different Explants and Growth Regulators on In vitro Regeneration of Chrysanthemum

2020 ◽  
pp. 10-18
Author(s):  
Md. Nazmul Hasan Mehedi ◽  
Nurunnahar Mitu ◽  
Mahbub Robbani ◽  
Kazi Foyjun Nesa Sukhi ◽  
Md. Hasan Sofiur Rahman ◽  
...  
Keyword(s):  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Shoot Tip ◽  
Ms Medium ◽  
Apical Shoot ◽  
Standardized Protocol ◽  
Best Response ◽  
The Family ◽  
Number Of Leaves

Chrysanthemum is the world’s second most economically important flower crop and commonly known as ‘Autumn Queen’. It belongs to the family Compositeae (Asteraceae). It is native to Asia and northeastern Europe and has been cultivated for more than 2000 years. The present study within vitro regeneration of chrysanthemum was carried out to develop the standardized protocol for organogenesis. In this study, three types of explants viz. apical shoot tip, internodal segment and young leaf along with different concentrations and combinations of growth regulators were used for in vitro regeneration. BAP and KIN were used for in vitro microshoot regeneration and IBA along with 2, 4-D were used for in vitro microroot regeneration. Minimum days (7.00) for microshoot initiation, maximum microshoot initiation percentage (97.00), highest number of microshoot per plantlet (12.00), highest number of leaves per microshoot (14.60) and maximum microshoot length (4.60) at 28 DAC were recorded as best performances by apical shoot tip inoculated into MS medium supplemented with BAP 2.5 mg/L + KIN 0.5 mg/L. On the other hand, minimum days (5.00) for microroot initiation, maximum microroot initiation percentage (97.60), the highest number of microroots per plantlet (11.80) and maximum microroot length (6.20) were obtained from apical shoot tip inoculated into ½ strength MS medium supplemented with IBA 0.2 mg/L + 2, 4-D 0.1 mg/L. In case of microshoot regeneration, the best response was showed by apical shoot tip when inoculated into MS medium supplemented with BAP 2.5 mg/L + KIN 0.5 mg/L and the microrooting of plantlets were best from apical shoot tip inoculated into ½ MS medium supplemented with IBA 0.2 mg/L along with 2, 4-D 0.1 mg/L.

scholarly journals In Vitro Regeneration of Dendrobium sp. of Orchid Using Leaf Tip as Explant

2016 ◽  
Vol 8 (2) ◽  
pp. 75-78
Author(s):  
K Goswami ◽  
S Yasmin ◽  
KM Nasiruddin ◽  
F Khatun ◽  
J Akte
Keyword(s):  
In Vitro Regeneration ◽  
Plantlet Regeneration ◽  
Shoot Length ◽  
Ms Medium ◽  
Fresh Weight ◽  
Half Strength ◽  
Maximum Root Length ◽  
Maximum Root ◽  
Regenerated Plantlets

Plant growth regulators (PGRs) namely, 2,4-D, NAA and BAP were added into Murashige and Skoog (MS) medium to observe the effect of PGRs on the growth and development of Dendrobium sp. orchid. Leaf tips of Dendrobium sp. were used as explants and inoculated on MS medium supplemented with 2, 4 D (0, 0.5, 2.5, 5, 10 mgL?1) for development of PLBs. The maximum PLBs formation (90%) and the maximum number of PLBs (16.00) were observed in 10 mgL?1 2, 4-D into MS medium after 60 days of culture. Subcultured PLBs were inoculated on MS medium supplemented with different combinations of NAA (0, 0.5, 2.5, 5 mgL?1) and BAP (0, 0.5, 2.5, 5 mgL?1) for shoot regeneration. The maximum number of shoot (11.00), the highest fresh weight (0.6233g) and the highest shoot length (3.613 cm) were observed in 0.5 mgL?1 NAA + 0.5 mgL?1 BAP after 60 days of culture. Even, the maximum number of root (4.00), the maximum root length (1.627cm) and the maximum plantlet regeneration percentage (93.33%) were observed with the combined effect of 0.5 mg NAA and 0.5 mg BAP after 60 days of culture. Finally, regenerated plantlets were transferred into half strength MS medium to obtain plants.J. Environ. Sci. & Natural Resources, 8(2): 75-78 2015

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