Study On The Cross Compatibility Of Some Lemon Genotypes (<i>Citrus Limon</i> L.)

An investigation was carried out at the Department of Horticulture, Bangladesh Agricultural University, Mymensingh to examine compatible relationship among the available lemon genotypes. Seven selected genotypes of lemon were used for hybridization. Crossings were performed following diallel fashion. The results revealed that the lower percentage of fruit setting as well as seed setting in some cross combinations noticed the existence of incompatibility among the selected genotypes. The percentages of fruit setting and seed setting were higher in the cross-pollination than in the self- pollination, which was an indication of self-incompatibility. To achieve seedless fruit setting, self-incompatibility may be used successfully. Keywords: Cross compatibility; lemon genotypes. DOI: http://dx.doi.org/10.3329/bjar.v36i2.9250 BJAR 2011; 36(2): 241-246

Download Full-text

Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽

10.3390/genes12030432 ◽

2021 ◽

Vol 12 (3) ◽

pp. 432

Author(s):

Yaling Chen ◽

Benchang Hu ◽

Fantao Zhang ◽

Xiangdong Luo ◽

Jiankun Xie

Keyword(s):

Pollen Tubes ◽

The Self ◽

Dendrobium Officinale ◽

Self Incompatibility ◽

Related Gene ◽

Cytological Observation ◽

Lectin Receptor ◽

Cross Pollination ◽

Self Pollination ◽

Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

Download Full-text

Reproductive Success after Self-pollination and Cross-pollination of Olive Cultivars in Croatia

HortScience ◽

10.21273/hortsci.46.2.186 ◽

2011 ◽

Vol 46 (2) ◽

pp. 186-191 ◽

Cited By ~ 22

Author(s):

Gabriela Vuletin Selak ◽

Slavko Perica ◽

Smiljana Goreta Ban ◽

Mira Radunic ◽

Milan Poljak

Keyword(s):

Reproductive Behavior ◽

Fruit Set ◽

Positive Response ◽

Flowering Period ◽

Self Incompatibility ◽

Cross Pollination ◽

Self Pollination ◽

Cross Compatibility ◽

Olive Cultivars ◽

Simultaneous Flowering

Olive orchard productivity largely depends on the choice of planted cultivars and their pollination needs. Orchard designs in Croatia are changing because a number of valuable foreign olive cultivars, mostly Italian, have been introduced in this region in the last 30 years. The compatibility relationships of introduced cultivars with autochthonous cultivars are unknown. With the objective of studying reproductive behavior of the most important Croatian cultivars (Drobnica, Lastovka, Levantinka, and Oblica) and their cross-pollination to recently introduced Italian cultivars Leccino and Pendolino, initial and final fruit set in self-pollination versus cross-pollination and free pollination were compared during three flowering seasons. Experiments were conducted in three different orchards (Kastela, Mravince, and Brac) to identify the effect of the environment on reproductive behavior of olive cultivars. The differences of fruit set in five olive cultivars after tested pollination treatments appeared at the time of initial fruit set. Increased final fruit set under cross-pollinations was observed when compared with self-pollination for all olive cultivars in all experimental orchards. In the Mravince orchard, a positive response to cross-pollination was consistent, and fruit set increased under cross-pollination in all cultivars and years with the exception of ‘Levantinka’ in which no significant differences were noticed between self-pollination treatment and cross-pollination treatments in 2005. Variable self-fertility behavior from season to season was found for tested cultivars. A self-incompatibility index (ISI) higher than 0.1 was recorded for ‘Levantinka’ in all experimental years and, therefore, classified it as a partially self-incompatible cultivar. Self-incompatibility response was observed for ‘Lastovka’. The positive response to cross-pollination over self-pollination only in some experimental years classified ‘Drobnica’, ‘Leccino’, and ‘Oblica’ as partially self-incompatible. Results obtained from this study indicated that pollination efficiency is strictly combination-specific. The Italian cultivar, Leccino, was a successful pollen acceptor and pollenizer of most Croatian cultivars. Reciprocal high success in cross-pollination was recorded for ‘Levantinka’ and ‘Oblica’. In the Mravince orchard, ‘Lastovka’, ‘Leccino’, and ‘Oblica’ were efficient pollenizers of ‘Levantinka’ where the simultaneous flowering period was in accordance with their cross-compatibility. ‘Levantinka’ was a good pollenizer for ‘Lastovka’ in the Mravince orchard, and both cultivars entered into the flowering period earlier than other studied cultivars, which was not the case in the other two orchards. The variations in flowering timing among orchards were a consequence of differences in environmental conditions. According to the high fruit sets recorded in ‘Oblica’ after pollination with ‘Leccino’ or ‘Levantinka’, an increase in tree productivity of the acceptor cultivar is expected in the presence of selected pollenizers in all olive-growing regions.

Download Full-text

Cross Compatibility Analysis to Identify Suitable Parents of Tagetes erecta and T. patula for Heterotic Hybrid Breeding

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47311388 ◽

2019 ◽

Vol 47 (3) ◽

Author(s):

Huali ZHANG ◽

Haibo XIN ◽

Richen CONG ◽

Zijing LI ◽

Lina SONG ◽

...

Keyword(s):

Plant Height ◽

Male Parent ◽

Hybrid Breeding ◽

Interspecific Crosses ◽

Tagetes Erecta ◽

Seed Setting ◽

Cross Compatibility ◽

Ray Florets ◽

The Cross ◽

Seed Setting Rate

Seven interspecific crosses of Tagetes erecta × T. patula were conducted in this study. The cross compatibility index, seed setting rate, germination rate, phenotype, ploidy level, heterosis over male parent and field performance were tested. The results showed that different cross compatibility indices of Tagetes vary greatly, from 10.44~114.41, which is far less than that of the intraspecific hybridization S-121 × I-506 index. The seed setting rate of each cross ranged from 15.67% to 49.0%, and the cross S-121 × TP-512 scored the highest seed setting rate, which was higher than that of the intraspecific cross S-121 × I-506 (35.85%). Compared with the male parent, F1 has higher plant height, wider crown width, larger flower diameter and more ray florets and earlier bloom with two exceptions. The S-121 of T. erecta was diploid. All of the male parents of T. patula were tetraploids, and the combinations were triploids. The traits of plant height, plant width, flower size and number of ray florets showed positive heterosis over the male parents, and the days leading to flowering showed both positive and negative ones. Finally, S-121 × TP-379 and S-121 × TP-512 were selected as the two best combinations. This study confirmed that the two species of marigold could be crossed for better F1 varieties with improved performance. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

Download Full-text

Late-acting self-incompatibility in tea plant (Camellia sinensis)

Biologia ◽

10.2478/s11756-012-0018-9 ◽

2012 ◽

Vol 67 (2) ◽

Cited By ~ 11

Author(s):

Xuan Chen ◽

Shan Hao ◽

Li Wang ◽

Wanping Fang ◽

Yuhua Wang ◽

...

Keyword(s):

Pollen Tube ◽

Camellia Sinensis ◽

Pollen Tubes ◽

Tea Plant ◽

The Self ◽

Self Incompatibility ◽

Cross Pollination ◽

Self Pollination ◽

Hand Pollination ◽

Pollen Tube Elongation

AbstractThe self-incompatibility of tea plant (Camellia sinensis (L.) O. Kuntze) was studied with the methods of aniline blue fluorescence assay and paraffin sections. The characteristics of pollen tube elongation after hand pollination was analyzed in 4 tea cultivars, including ‘Keemenzhong’, ‘Longjing-changye’, ‘Fuding-dabaicha’ and ‘Yabukita’, under self-pollination and cross-pollination, respectively. Although there were some difference among cultivars, pollen tubes elongated through the style and reach the ovary successfully at 48 h after pollination for both cross- and self-pollen tubes in all the four cultivars of tea. Pollen tubes entered into the ovule micropyles, however, only for cross-pollination, but not for self-pollination. Pollen tubes of selfing plants, failed in fertilizing, seemed have some difficulties to enter the ovule. All of which indicated that the self-incompatibility of tea plant is a late-acting self-incompatibility system (LSI) or an ovarian sterility (OS), in which the self incompatibility was due to none self pollen tube penetrating into the ovule and no fertilization.

Download Full-text

Self-incompatibility Expression in Self-compatible Almond Genotypes May Be Due to Inbreeding

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.130.6.865 ◽

2005 ◽

Vol 130 (6) ◽

pp. 865-869 ◽

Cited By ~ 13

Author(s):

José Manuel Alonso ◽

Rafael Socias i Company

Keyword(s):

Pollen Tube ◽

Differential Expression ◽

Pollen Tube Growth ◽

Pollen Tubes ◽

Lower Number ◽

The Self ◽

Lower Percentage ◽

Self Incompatibility ◽

Prunus Amygdalus ◽

Self Compatibility

Pollen tube growth after selfing was studied in four almond (Prunus amygdalus Batsch) families derived from crosses between self-compatible `Tuono' and self-incompatible `Ferragnès' and `Ferralise' in both directions, in order to ascertain the phenotypic expressions of the different genotypes. A differential expression of self-compatibility was observed in the seedlings of the different families. The genetic self-compatible offspring of `Ferralise' showed a lower percentage of pistils with pollen tubes at the style base and a lower number of pollen tubes at the pistil base after self-pollination than those observed in the self-compatible offspring of `Ferragnès'. This low level of self-compatibility expression observed in some `Ferralise' seedlings may be due to the inbreeding present in `Ferralise'. As a consequence, caution must be taken in almond breeding to avoid the increase of inbreeding by the utilization of related parents and to diversify the sources of self-compatibility, at present mostly limited to `Tuono.'

Download Full-text