scholarly journals In vitro micropropagation in tropical short day onion (Allium cepa L.)

2018 ◽  
Vol 47 (4) ◽  
pp. 961-967
Author(s):  
Rupali Passi ◽  
AS Dhatt ◽  
MK Sidhu
Keyword(s):  
Mercuric Chloride ◽  
Growth Hormones ◽  
Root Induction ◽  
Ms Medium ◽  
Short Day ◽  
In Vitro Micropropagation ◽  
Allium Cepa L ◽  
In Vitro Establishment ◽  
Tropical Varieties

For in vitro micropropagation, short day tropical varieties of onion ‘Agrifound Dark Red’, ‘Punjab Naroya’ and ‘PRO-6’ were exposed to different concentrations and combinations of growth hormones. Pre-sterilization of basal plate of onion in 0.5% solution of bavistin followed by treatment with 0.1% mercuric chloride for 10 min. produced the highest rate of survival of explants (47.9%). Survival was further enhanced to 53.17% with the addition of 750 ppm cefotaxime in MS medium. Varietal differences were observed for in vitro establishment, multiplication and root induction. Among various combinations of growth hormones, MS medium supplemented with 4.0 mg/l BAP + 0.5 mg/l NAA, 2 mg/l BAP and 0.5 mg/l NAA and half MS carrying 1.0 mg/l IBA + 0.5 mg/l NAA produced the highest in vitro establishment (53.07%), multiplication (64.46%) and rooting (66.37%) respectively, in ‘Agrifound Dark Red’.

scholarly journals In vitro micropropagation of pointed gourd (Trichosanthes dioica Roxb.) from shoot tip and nodal segment

2012 ◽  
Vol 47 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Amdadul Huq ◽  
Shahina Akter ◽  
Shahina Islam ◽  
Salim Khan
Keyword(s):  
Shoot Regeneration ◽  
Garden Soil ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Best Response ◽  
In Vitro Micropropagation ◽  
Trichosanthes Dioica ◽  
Pointed Gourd

An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in pointed gourd. Highest percent of shoot regeneration was 91.66%, when nodal explants were cultured on MS+1.0 mg/1 BAP. The maximum number of shoots (4.8) per explant was observed in MS + 1.0 mg/1 BAP + 0.2 mg/1 NAA from nodal segment. Among the two explants, nodal segment was found better for shoot regeneration and multiplication. The best response towards root induction was achieved on half MS medium supplemented with 0.4 mg/1 NAA. The regenerated healthy rooted plantlets were transferred to small plastic pot containing garden soil and compost in a ratio of 2:1. Immediately after transplantation the plantlets were covered with polythene bag to prevent desiccation. After acclimatization the plantlets were successfully transferred to the garden soil and the percentage of survivability in such condition was 90%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11457   Bangladesh J. Sci. Ind. Res. 47(2), 217-222, 2012  

scholarly journals Optimization of Modified Murashige and Skoog (MS) Medium Promoting Efficient Callus Induction in Zinnia elegans

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Utkalendu Suvendusekhar Samantaray ◽  
Tanima Singh
Keyword(s):  
Callus Induction ◽  
Large Scale ◽  
Growth Hormones ◽  
Zinnia Elegans ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
In Vitro Micropropagation ◽  
Rapid Growth Rate ◽  
2,4 Dichlorophenoxyacetic Acid

Zinnia (Zinnia elegans Jacq.) occupies a pristine place in the garden for its bright colours and long flowering period. Zinnias are propagated by seeds hence genetic variation and a tendency for stock plants to decline in vigor over time is high. Young internodal explants of this species were grown on modified Murashige and Skoog (MS) medium supplemented with various growth hormones to evaluate callus induction. 2,4 dichlorophenoxyacetic acid (2,4-D) at 1.0 mg/L was shown to be the best choice for a high percentage of callus induction (70-73%) and a rapid growth rate of viable and healthy callus. A large-scale and quick callus generation protocol was developed in the lab and might be used for in-vitro micropropagation.

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
J. D. BARSHILE
Keyword(s):  
Shoot Tip ◽  
Root Induction ◽  
Multiple Response ◽  
Ms Medium ◽  
Growth Responses ◽  
Rapid Multiplication ◽  
Micro Propagation ◽  
G 12 ◽  
Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

scholarly journals In vitro establishment of blackberry (Rubus sp.) cultivar 'Xavante'

2016 ◽  
Vol 46 (9) ◽  
pp. 1542-1545
Author(s):  
Tânia Regina Pelizza ◽  
Fabiane Nunes Silveira ◽  
Roberta Sabatino Ribeiro ◽  
Bruno Dalazen Machado ◽  
Leo Rufatto ◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
Ms Medium ◽  
Lower Survival ◽  
In Vitro Establishment

ABSTRACT: The objective of this study was to evaluate the in vitro establishment of 'Xavante' blackberry under different conditions of plant luminosity and concentrations of salts of MS medium. The maintenance of 'Xavante' blackberry plants in the absence of light, with the use of MS medium with salt concentrations of 75% and 100% reduce bacterial contamination and the percentage of explants oxidized and provide the highest percentage of surviving and established explants. The 50% salt dilution in MS medium is not recommended when plants are subjected to the absence of light due to the high percentage of oxidized explants and consequently lower survival and establishment.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Research on in vitro micropropagation of Lilium brownii F.E. Brown

2016 ◽  
Vol 14 (1) ◽  
pp. 121-129
Author(s):  
Vũ Hoài Sâm ◽  
Bùi Đức Quỳnh ◽  
Nguyễn Thị Hương ◽  
Nguyễn Văn Khiêm
Keyword(s):  
Subtropical Climate ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Medicinal Species ◽  
Surface Sterilization ◽  
The North ◽  
In Vitro Micropropagation ◽  
Half Strength ◽  
Over Exploitation

Lilium brownii Brown belonging Lilium genus and Liliaceae family is well-known as a popular medicinal species, as well as food source and beautiful ornamental flowers. The specie has unique and ornamental floral characteristics such as light and elegant fragrance and perianth color rapidly changing from yellowish cream to white during anthesis. In traditional medicine, it is used for treatment cough, sedation diuretic, bronchitis... In nature, it can be found in subtropical climate moutainous areas in the North such as Sa Pa, Bat Xat, Mu Cang Chai; Sin Ho and Phong Tho, Quang Ba and Dong Van. In recent years, this species has been listed in the Red List for medicinal plants in Vietnam due to over-exploitation. The only effective strategy for sustaible conservation this species is in vitro micropropagation. In this study, in vitro plant regeneration and micropropagation of L. brownii was established from bubles and stem nodes. After surface sterilization with 0.1% HgCl­2 in 10 minutes, healthy young shoots were obtained from initial bubles and stem nodes on MS medium supplemented with 0.5 mg/l BAP or 0.5 mg/l NAA, respectively.  Bulblets also were formed from young shoot on MS supplemented with 0.5 mg/l NAA. The highest number of 4.5 bulblets per an explant was recorded from longitude-divided bubbles on MS medium containing 0.5 mg/l NAA and 0.2 mg/l BAP after 60 days in culture. The regererated plants produced quality roots on half strength MS supplemented with the combination of 1.0 mg/ l NAA and 0.2 mg / l BAP. More than 90% of rooted plants in vitro were survival on artificial soil TN1 in the nursery.

scholarly journals Micropropagation’s Complete Protocol of Red Araçá (Psidium cattleianum, Myrtaceae) from Germinated Seeds in vitro

2018 ◽  
Vol 10 (2) ◽  
pp. 234
Author(s):  
Cassio G. Freire ◽  
João P. P. Gardin ◽  
Cesar M. Baratto ◽  
Renato L. Vieira ◽  
Simone S. Werner
Keyword(s):  
Food Production ◽  
High Rate ◽  
Nodal Segments ◽  
Ms Medium ◽  
Brazilian Atlantic Forest ◽  
Psidium Cattleianum ◽  
Different Types ◽  
In Vitro Establishment ◽  
Germinated Seeds

Red Araçá’s (Psidium cattleianum) micropropagation processes have shown enormous potential both in terms of research and as a sustainable native resource to be used in the areas of food production, ecology, and pharmacology. Currently, however, despite that potential, research efforts involving this myrtaceae, native to the Brazilian Atlantic Forest, have been scarce. With that in mind, this study set out to establish micropropagation techniques that would allow the development of a feasible protocol to be used with Red Araçá, achieving its mircropropagation from in vitro germinated seeds. Different types of explants were tested for in vitro establishment. For the multiplication of nodal segments, different concentrations of BAP and IAA combinations were tested in an MS medium. Using the same medium, different concentrations of ampicillin were applied in order to determine its influence on the decontamination of the apical segments. The BAP and IAA combinations were also used to test their effects on the in vitro explants’ development and rooting. During pre-acclimatization, survival of in vitro rooted plants was tested in a nebulizer chamber, using a commercial substrate and that same substrate mixed with washed sand (1:1). In essence, it was indeed possible to develop a complete protocol for the micropropagation of the Red Araçá from seedlings obtained by in vitro germination. The in vitro introduction of the Red Araçá was rather efficient, independently of the type of explants used. As the BAP and IAA concentrations increased, so did the in vitro seedlings’ development (7 leaves explant-1) and rooting (67%). Additionally, the in vitro rooted plants exhibited a high rate of survival (80%) in the pre-acclimatization phase, independently of the substrate used.

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

scholarly journals In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

1970 ◽  
Vol 17 ◽  
pp. 139-144 ◽  
Author(s):  
MS Rahman ◽  
MF Hasan ◽  
R Das ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

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