The expression of BCL-G in leukemia and gastrointestinal tissues

Background & Objective:- BCL-G is a novel protein of Bax (BCL-G associated protein X) that induces caspase-mediated apoptosis. These proteins play important roles in regulating apoptosis in both normal or neoplastic cells, however their cellular and tissue distribution remains to be determined. Thus, the current study objective was to elucidate the distribution and expression pattern of BCL-G in normal and malignant gastrointestinal human tissues.Methods:- The distribution and expression of BCL-G was measured by immunohistochemistry using a rabbit monoclonal antibody against BCL-G in formalin-fixed, paraffin embedded, benign or malignant human tissue.Results:- A variable pattern of positive expression of BCL-G was observed within all the tissues studied. BCL-G expression was found to be localized to the cytoplasmic paranuclear granules of the epithelialium in the majority of organs examined. Intensity of BCL-G staining was associated withthe maturation state of benign tissue.Conclusion:- Here we demonstrate that BCL-G exhibits a specific tissue distribution pattern that appears to correlate with cellular differentiation. While such distribution patterns are complex they provide an intriguing insight into overall function that would require further investigation to fully elucidate their physiological/pathological significance.Bangladesh Journal of Medical Science Vol.16(3) 2017 p.433-438

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Anti-CD8 (SP57) rabbit monoclonal antibody is a useful tool for detecting Toxoplasma gondii on formalin-fixed, paraffin-embedded tissue sections

Human Pathology ◽

10.1016/j.humpath.2015.09.047 ◽

2016 ◽

Vol 52 ◽

pp. 197-198

Author(s):

Laure Manfredi ◽

Emmanuelle Uro-Coste ◽

Pierre Brousset

Keyword(s):

Monoclonal Antibody ◽

Toxoplasma Gondii ◽

Tissue Sections ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Rabbit Monoclonal Antibody ◽

Formalin Fixed

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Quality of DNA extracted from formalin-fixed, paraffin-embedded canine tissues

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638720929637 ◽

2020 ◽

Vol 32 (4) ◽

pp. 556-559

Author(s):

Jonathan D. Dear ◽

Jane E. Sykes ◽

Danika L. Bannasch

Keyword(s):

Whole Blood ◽

Tissue Banks ◽

Call Rate ◽

Blood Samples ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Veterinary Pathology ◽

Whole Blood Samples ◽

Specific Tissue ◽

Formalin Fixed

Veterinary pathology tissue banks are valuable resources for genetic studies. However, limited data exist as to whether quality DNA can be extracted from these tissues for use in canine genotyping studies. We extracted DNA from 44 formalin-fixed, paraffin-embedded (FFPE) tissue blocks from dogs; 9 of these dogs had DNA available from whole blood samples that had been banked. We genotyped DNA from 30 of 44 tissue blocks and 9 whole blood samples on the Illumina CanineHD BeadChip; DNA quality was insufficient in 14 of 44 samples from tissue blocks. There was significant correlation between the 260/280 ratio and single-nucleotide variation (SNV) call rate ( p = 0.0276; r2 = 0.162); 23 of 30 samples from FFPE were genotyped with > 65% call rates. Median pairwise identical-by-state (IBS) analysis was 0.99 in 8 pairs of dogs with call rates > 65%. Neither age of tissue block nor specific tissue types were associated with significant differences in DNA concentration, 260/280 ratio, or SNV call rate. DNA extracted from tissue blocks can have variable quality, although comparable levels of homozygosity suggest that extracts from FFPE with call rates > 65% might provide similar results to samples from whole blood when analyzed on the Illumina CanineHD BeadChip.

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