scholarly journals Antioxidant, Membrane Stabilizing and Cytotoxic Activities of Cissus adnata (Roxb.)

2016 ◽  
Vol 15 (1) ◽  
pp. 69-71 ◽  
Author(s):  
Ridwan Bin Rashid ◽  
Farhan Nur Towsif ◽  
Farhana Ahmed Bushra ◽  
Faiza Tahia
Keyword(s):  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cytotoxic Activities ◽  
Hypotonic Solution ◽  
Lc50 Value ◽  
Whole Plant ◽  
Vincristine Sulphate

The methanol extracts of whole plant of Cissus adnata (Roxb.) and their organic and aqueous soluble partitioning materials were evaluated for antioxidant, cytotoxic and membrane stabilizing properties. In the DPPH free radical scavenging assay, the aqueous extract of whole plant of C. adnata displayed the highest free radical scavenging activity with IC50 value of 4.17 ?g/ml, while the pet ether soluble fraction showed significant lethality to brine shrimps having LC50 value of 1.02 ?g/ml, as compared to the standard vincristine sulphate (LC50 value of 0.44 ?g/ml). In the evaluation for membrane stabilizing property, the pet ether soluble fraction of methanol extract of the whole plant inhibited 67.43 % and 56.60 % hypotonic solution- and heat-induced haemolysis of RBCs, respectively.Dhaka Univ. J. Pharm. Sci. 15(1): 69-71, 2016 (June)

scholarly journals Wild Cinchona (Neolamarckia cadamba) – Bioactivities of a Medicinal Plant of Bangladesh

2016 ◽  
Vol 19 (1) ◽  
pp. 32-36
Author(s):  
Faiza Tahia ◽  
Mohidur Rahman ◽  
Marzia Bilkiss ◽  
Md Al Amin Sikder ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Vincristine Sulphate

The methanol extract of bark of Neolamarckia cadamba (Roxb.) and its organic and aqueous soluble partitionates were subjected to assays for antioxidant, cytotoxic, thrombolytic, membrane stabilizing, antimicrobial and analgesic activities. In the DPPH free radical scavenging assay, the pet ether and carbon tetra chloride soluble partitionate of the methanolic extract demonstrated the highest free radical scavenging activity with IC50 values of 60.46 ?g/ml and 78.58 ?g/ml, respectively. On the other hand, the carbon tetrachloride and pet ether soluble fractions displayed the potential lethality to brine shrimps, having LC50 of 7.24 and 7.89 ?g/ml, as compared to standard vincristine sulphate (LC50 value of 0.45 ?g/ml). During assay for thrombolytic property, the carbon tetrachloride and aqueous soluble materials revealed 66.36 % and 64.25 % clot lysis of human blood, respectively. In the membrane stabilizing assay, the carbon tetrachloride soluble fraction inhibited 86.79 % haemolysis of human RBCs in hypotonic solution-induced condition, while the aqueous soluble partitionate inhibited 92.39 % haemolysis of RBCs in the heat-induced condition. The crude methanolic extract of bark of N. cadamba showed significant central and peripheral analgesic activity at both 200 and 400 mg/kg body weight, whereas its chloroform soluble fraction mildly inhibited the growth of test microorganisms. Therefore, our studies suggest that wild cinchona should be subjected to extensive phytopharmacological investigation.Bangladesh Pharmaceutical Journal 19(1): 32-36, 2016

scholarly journals Bioactivities of Malvaviscus arboreus var. drummondii and Phyllanthus reticulatus Poir.

2015 ◽  
Vol 13 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Zokhroof Yeasmin ◽  
Sharif Tanvir ◽  
Tasnuva Sharmin ◽  
Ridwan Bin Rashid ◽  
Md Al Amin Sikder ◽  
...  
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Activity Assay ◽  
Vincristine Sulphate ◽  
Crude Methanol Extract

The study was designed to evaluate the bioactivities of crude methanol extracts of leaves of Malvaviscus arboreus and Phyllanthus reticulatus along with their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates. The antioxidant potential was evaluated by DPPH and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. In the DPPH free radical scavenging assay, the crude methanol extract of M. arboreus revealed the highest free radical scavenging activity with IC50 values 6.47 ± 0.78 ?g/ml. In brine shrimp lethality bioassay, the chloroform soluble materials of M. arboreus displayed the highest cytotoxic potential having LC50 values 2.73 ± 0.36 ?g/ml, whereas the standard vincristine sulphate exhibited an LC50 value of 0.45 ?g/ml. In the thrombolytic activity assay, the pet-ether soluble fractions of M. arboreus and P. reticulatus revealed 55.60 ± 0.48 % and 18.99 ± 0.84% clot lysis, respectively. In the membrane stabilizing activity assay, the chloroform soluble fraction of M. arboreus inhibited heat- and hypotonic solution- induced haemolysis of RBCs by 54.60 ± 0.81 % and 66.96 ± 0.84 % as compared to 57.06 % and 72.79 % inhibition by acetyl salicylic acid (0.10 mg/ml), respectively. On the other hand, the pet-ether soluble materials of crude methanol extract of P. reticulatus demonstrated 57.11 ± 0.57 % and 70.62 ± 0.22 % inhibition of heat- and hypotonic solution- induced hemolysis, respectively. DOI: http://dx.doi.org/10.3329/dujps.v13i2.21892 Dhaka Univ. J. Pharm. Sci. 13(2): 143-147, 2014 (December)

scholarly journals In vitro Bioactivities of Three Reputed Medicinal Plants of Bangladesh

2015 ◽  
Vol 17 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Abu Bakar Siddique ◽  
Ridwan Islam ◽  
Md Al Amin Sikder ◽  
Ridwan Bin Rashid ◽  
AKM Nawshad Hossian ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Free Radical ◽  
Soluble Fraction ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Acetyl Salicylic Acid ◽  
Total Phenolic ◽  
Hypotonic Solution

The study was designed to evaluate the preliminary biological activities of crude methanol extracts of the leaf of Justicia gendarussa, Sansevieria trifasciata and Hydnocarpus kurzii and their organic soluble partitionates. The polyphenol content was determined spectrophotometrically and expressed as gallic acid equivalents. The total phenolic content was found to vary for different test samples ranging from 1.45 ± 0.25 mg to 40.73 ± 0.22 mg of GAE/ gm of dried extract. The antioxidant potential was evaluated by DPPH free radical scavenging assay using butylated hydroxytolune (BHT) and ascorbic acid as standards. In the assay, the CSF of H. kurzii and the PESF of J. gendarussa revealed the highest free radical scavenging activity with IC50 values 3.25 ± 0.05 ?g/ml and 24.68 ± 0.26 ?g/ml, respectively. The brine shrimp lethality bioassay was utilized to evaluate the cytotoxicity. The chloroform soluble fraction (CSF) and methanol extract (ME) of J. gendarussa exhibited strong cytotoxicity with LC50 values of 0.002 ?g/ml and 0.06 ?g/ml, respectively. The membrane stabilizing activity was assessed by evaluating hemolysis of RBC in hypotonic solution and was compared with acetyl salicylic acid. On the other hand, the chloroform soluble fraction of J. gendarussa and aqueous soluble materials of S. trifasciata produced 75.60% and 75.0% inhibition of hemolysis of RBC, respectively as compared to 77.9% inhibited by acetyl salicylic acid (0.10 mg/ml). DOI: http://dx.doi.org/10.3329/bpj.v17i2.22331 Bangladesh Pharmaceutical Journal 17(2): 147-150, 2014

scholarly journals Screenings of Four Medicinal Plants of Bangladesh for Bioactivities

2013 ◽  
Vol 12 (1) ◽  
pp. 59-62
Author(s):  
Md. Al Amin Sikder ◽  
Tasnuva Sharmin ◽  
AFM Mustafizur Rahman ◽  
Mohammad Rashedul Haque ◽  
Mohammad S Rahman ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Whole Plant

The methanol extracts of leaves of Nephelium lappaceum L., Pandanus foetidus Roxb., Ludwigia repens Rubin. and the whole plant of Adiantum philippense L. were subjected to assays of antioxidant, cytotoxic, thrombolytic and membrane stabilizing activities. The antioxidant activity was evaluated by DPPH free radical scavenging activity using butylated hydroxytolune and ascorbic acid as standards. The crude extract of N. lappaccam exhibited the highest free radical scavenging activity (IC50= 3.93±0.25?g/ml). On the other hand, the crude methanol extracts of A. philippense and P. foetidus demonstrated significant brine shrimp lethality with LC50 values 0.50±0.84 ?g/ml and 0.58±0.73 ?g/ml, respectively as compared to the standard anti-neoplastic drug, vincristine sulfate (LC50= 0.45 ?g/ml). Weak thrombolytic activity was observed for the test samples. The crude methanol extracts of P. foetidus and N. lappaceum showed 17.31±0.82 % and 17.29±0.91 % clot lysis while standard streptokinase and water, used as positive and negative controls, demonstrated 66.77 % and 3.79 % lysis of clot, respectively. The methanol extract of whole plant of A. philippense inhibited hypotonic solution and heat induced haemolysis of RBCs by 53.09±1.01 % and 35.36±1.60 % as compared to 71.77 % and 42.20 % by acetyl salicylic acid, respectively. Dhaka Univ. J. Pharm. Sci. 12(1): 59-62, 2013 (June) DOI: http://dx.doi.org/10.3329/dujps.v12i1.16301

scholarly journals Studies of Bioactivities of Adansonia digitata (L.)

2016 ◽  
Vol 14 (2) ◽  
pp. 157-162
Author(s):  
Faiza Tahia ◽  
Priyanka Majumder ◽  
Md Al Amin Sikder ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Adansonia Digitata ◽  
Lc50 Value ◽  
Vincristine Sulphate

The methanol extracts of leaves and barks of Adansonia digitata (L.) and their organic and aqueous soluble partitioning materials were evaluated for antioxidant, cytotoxic, thrombolytic, membrane stabilizing, antimicrobial, analgesic, and antidiarrhoel activities. In the DPPH free radical scavenging assay, the aqueous soluble materials of bark and leaves of A. digitata displayed the highest free radical scavenging activity with IC50 values of 2.21 ?g/ml and 7.70 ?g/ml, respectively while the crude extract of leaves and its pet ether soluble fraction showed significant lethality to brine shrimps having LC50 value of 6.99 and 0.284 ?g/ml, respectively as compared to standard vincristine sulphate (LC50 value of 0.44 ?g/ml). During assay for thrombolytic activity of human blood clot, the carbon tetrachloride soluble partitionate of leaves of A. digitata revealed 48.11% clot lysis. In the membrane stabilizing assay, the carbon tetrachloride- and aqueous-soluble fractions of methanol extract of leaves inhibited 61.52 % and 16.03 % hypotonic solution- and heat-induced haemolysis of RBCs, respectively. The methanolic extracts of leaves and barks of A. digitata revealed significant central and peripheral analgesic activity at 200 and 400 mg/kg body weight. The crude extract of leaves of A. digitata also displayed significant antidiarrhoel but mild antibacterial activities.Dhaka Univ. J. Pharm. Sci. 14(2): 157-162, 2015 (December)

scholarly journals Preliminary Free Radical Scavenging, Brine Shrimp Lethality, Antimicrobial and Thrombolytic Activities of Aganosma dichotoma (Roth) K. Schum.

2015 ◽  
Vol 17 (2) ◽  
pp. 177-181
Author(s):  
Sanjoy Chandra Dey ◽  
Mohammd Firoz Khan ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Pharmaceutical Journal ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Brine Shrimp Lethality

Bangladesh is a good repository of natural products. Numerous plants are available to facilitate the traditional treatments. Proper scientific evaluations are essential to explore the plant derived drugs. With this view, the crude methanol extract of leaves of Aganosma dichotoma (Roth) K. Schum. and its Kupchan fractions were screened for free radical scavenging, brine shrimp lethality, antimicrobial and thrombolytic activities. Among all extractives, the chloroform soluble fraction demonstrated the highest free radical scavenging activity with IC50 value 18.21 ?g/ml. Moreover, the chloroform soluble fraction showed significant brine shrimp lethality having LC50 value of 3.98 ?g/ml. On the other hand, the petroleum ether, carbon tetrachloride and chloroform soluble materials revealed mild to moderate antimicrobial activity with the zone of inhibition ranging from 7 to 14 mm. In the thrombolytic assay, the carbon tetrachloride soluble partitionate showed the highest clot lysis (30.48%). This is the first report of the comprehensive investigations of different extractives of A. dichotoma for the abovementioned bioassays. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22337 Bangladesh Pharmaceutical Journal 17(2): 177-181, 2014

scholarly journals Antimicrobial, Cytotoxic, Thrombolytic and Antioxidant Activities of Syzygium fruticosum (Roxb.) DC.

2015 ◽  
Vol 17 (1) ◽  
pp. 51-54
Author(s):  
Somaia Haque Chadni ◽  
Abdullah Al Hasan ◽  
ATM Zafrul Azam
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Zone Of Inhibition ◽  
Aqueous Fraction

The crude methanol extracts of the bark and leaves of Syzygium fruticosum (Roxb.) DC. were partitioned with petroleum ether, carbon tetrachloride, chloroform and water for biological investigation. In the brine shrimp lethality bioassay, carbon tetrachloride soluble fraction of leaf extract (LCTF) showed significant lethality having the LC50 value 0.65 ?g/ml. In free radical scavenging activity screening (DPPH assay), chloroform fraction of methanolic extract of bark of S. fruticosum showed the highest free radical scavenging activity with IC50 value 20.01 ?g/ml. In the microbiological investigation, only chloroform soluble fraction of bark (BCF) and aqueous fraction of bark (BAF) showed mild antimicrobial activity with zone of inhibition ranging from 7 to 14 mm as compared to standard ciprofloxacin (zone of inhibition of 50 mm). In the study for thrombolytic property, different extracts of S. fruticosum revealed varying degrees of thrombolytic activity ranging from 33.46% to 62.51%. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22315 Bangladesh Pharmaceutical Journal 17(1): 51-54, 2014

scholarly journals Antioxidant, Antimicrobial, Anti-diarrheal and Analgesic Activities of Diospyros malabarica (Desr.) Kostel

2019 ◽  
Vol 22 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Md Moniruzzaman ◽  
Md Ruhul Kuddus ◽  
AM Sarwaruddin Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Tail Flick ◽  
Dependent Manner ◽  
Analgesic Activities

The present study was designed to evaluate the antioxidant, antimicrobial, antidiarrheal and analgesic activities of methanol extract of Diospyros malabarica (Desr.) Kostel buds growing in Bangladesh. The total phenolic content of methanol extract of D. malabarica and its Kupchan fractions was determined and expressed in gallic acid equivalent (GAE). In the DPPH free radical scavenging assay, the aqueous soluble fraction of the methanol extract of D. malabarica revealed maximum free radical scavenging activity having IC50 value of 6.17 μg/ml. In antimicrobial assay, all the test samples displayed prominent antimicrobial activity against the test organisms under in vitro conditions. Among these, the carbon tetrachloride soluble fraction was found to exhibit the highest activity against Bacillus cereus, Salmonella Typhi, Salmonella Paratyphi, and Candida albicans with the zone of inhibition as 32, 30, 28 and 30 mm, respectively. In evaluation of antidiarrheal activity, the D. malabarica extracts showed significant anti-diarrheal potential in a dose dependent manner. During the evaluation of analgesic activity by radiant heat tail-flick method, the plant extract at 400 mg/kg b.w. exhibited highest elongation (373.04%) as compared to morphine (472.48%). On the other hand, in acetic acid-induced writhing test, the extract at 200- and 400-mg/kg b.w. showed 61.11 and 66.67% inhibition of writhing in mice model, respectively as compared to 68.06% inhibition produced by the standard diclofenac-Na. The findings of this study justify some of the traditional uses of D. malabarica and reveal the bioactivity of the plants. Further studies are required to isolate and identify the bioactive compounds. Bangladesh Pharmaceutical Journal 22(1): 27-33, 2019

In Vitro Preliminary Phytochemical Screening and Free Radical Scavenging Ability of Drosera indica L.

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
K. R. Asha ◽  
S. Hemmalakshmi ◽  
S. Priyanga ◽  
K. Devaki
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Whole Plant

Aim: The present study is carried out to explore the preliminary phytochemical screening and free radical scavenging activity of the whole plant Drosera indica L. Methods: a) Phytochemical screening – The qualitative analysis of secondary metabolites is carried out by the standard qualitative methods. b) In vitro free radical scavenging activity of the ethanolic and aqueous extract of the whole plant Drosera indica L is used for the analysis .Various concentrations (100 – 500mcg/ml) of the ethanol and aqueous extracts of Drosera indica L. are used in the various antioxidant assay methods such as reducing power, ferric reducing antioxidant power assay (FRAP), nitric oxide (NO) radical,2,2’ azinobis-3 ethylbenzothiozoline-6 sulfonic acid (ABTS+) radical, hydroxyl radical (OH.), 1,1-diphenyl-2-picryl hydroxyl (DPPH) radical , super oxide radical and hydrogen peroxide (H2O2) is carried out with the standard protocols. In all the assays ascorbic acid is used as the standard antioxidant. Results: Phytochemical screening of the plants reveal the presence of numerous chemicals including flavanoids, tannins, polyphenols, cardiac glycosides and saponins. The ethanolic extract of Drosera indica L. shows better ability to scavenge ,1,1-diphenyl-2-picryl hydroxyl( DPPH)radical, hydroxyl radical, hydrogen peroxide, nitric oxide radical and superoxide radical. FRAP and the reducing power abilities of the ethanolic extract is increased with the increase in concentration of the plant extract. Conclusion: The ethanolic extract of Drosera indica L. shows better ability to scavenge the free radicals than the aqueous extract. From this study, a conclusion is drawn that Drosera indica L. can have more beneficial effects with respect to the presence of many active secondary metabolites which may likely to combat with the oxidative stress diseases like diabetes, cancer, cardio-vascular diseases and in general boost the immune system.

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