scholarly journals Callus induction and cell culture of castor (Ricinus communis L. CV. Shabje)

2014 ◽  
Vol 20 ◽  
pp. 161-169
Author(s):  
MA Rahman ◽  
MA Bari
Keyword(s):  
Cell Culture ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Secondary Metabolite ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Ricinus Communis ◽  
Culture Technique ◽  
Culture Industry ◽  
Cell Culture Technique

Context: Tissue culture is an important tool in plant biotechnology that allows for an increase in biomass or metabolite production by utilizing several techniques in callus or cell cultures. Ricin is a toxic protein that can be extracted from the castor bean as secondary metabolite. The ricin has been used experimentally in medicine to kill cancer cell. We developed callus and cell culture technique for the possible extraction of ricin from the cell suspension culture of Ricinus communis. Objectives: The present investigation aimed to develop the cell culture technique of R. communis L. cv. Shabje and tried to establish a protocol for cell suspension culture of castor for possible extraction of ricin from cell extract. Materials and Methods: The hypocotyl explants of R. communis L. cv. Shabje were used as experimental materials. Cultured on Murashige and Skoog medium supplemented with different concentrations and combinations of BAP, NAA, 2,4-D and IAA for callus induction. For cell culture, the media were used without agar with different concentrations and combinations of these hormones. Results: For callus induction the combination of BAP 2.0 mg/l + 0.5 mg/l NAA showed the best performance but in case of cell culture the combination of BAP 2.0 mg/l and 0.2 mg/l NAA showed the best result. Conclusion: The present investigation clearly established and demonstrated the method of obtaining cell suspension culture and important secondary metabolite ricin could be obtained from cell suspension culture of R. communis L. holding promises to explore cell culture industry for ricin production. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17738 J. bio-sci.  20:  161-169, 2012

Purification of human monocyte-macrophages by a cell suspension culture technique

1980 ◽  
Vol 53 (1) ◽  
pp. 94-103 ◽  
Author(s):  
Henry C. Stevenson ◽  
Paul Katz ◽  
Anthony S. Fauci
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Human Monocyte ◽  
Culture Technique ◽  
A Cell

scholarly journals Purification and partial characterization of white radish (Raphanus sativus L. var. Long white) peroxidase from cell suspension culture extract

2010 ◽  
pp. 1-16
Author(s):  
Sri Pudjiraharti ◽  
Andi Tenri Adjeng Karossi
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Raphanus Sativus ◽  
Deae Cellulose ◽  
Culture Technique ◽  
Enzyme Linked Immunosorbent Assay ◽  
Main Band ◽  
Raphanus Sativus L ◽  
White Radish

Peroxidase mainly Horseradish peroxidase (HRP) has been widely used as a component of clinical diagnostic reagent for Enzyme Linked Immunosorbent Assay (ELISA) technique. White radish (Raphanus sativus L.) was found as another source of peroxidase . In this study, white radish was used for the production of peroxidase by cell suspension culture technique. Isolation of the enzyme was conducted by ammonium sulfate precipitation followed by purification using DEAE-Cellulose column chromatography eluted with 0.01 M phosphate buffer, pH 7.5 and 0-0.5 M NaCl gradient. A major peak of protein having the highest activity and purity 25 folds compared to the crude enzyme was observed. This protein was partially characterized. SDS-Polyacrilamide gel electrophoresis showed one main band with molecular weight of 47.000 Da. This white radish peroxidase (WRP) is a very efficient enzyme with demonstrated maximum activity at temperature 55°C and pH 7.5 as well as a Km 76.6 μg/mL and Vmax 275 μg/mL/ minute toward hydrogen peroxide as substrate and pyrogallol as hydrogen donor.

scholarly journals Potential Embryogenic Callus Induction Protocol Through Cell Suspension Culture For High Frequency Plant Regeneration Of Maspine Pineapple (Ananas comosus L.)

1970 ◽  
Vol 3 (2) ◽  
pp. 40-45
Author(s):  
M.F. Mohamad Bukhori ◽  
Norzulaani Khalid ◽  
Ch'ng Lou Ven
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Embryogenic Callus Induction

To explore the potential for embryogenic callus induction protocol through cell suspension culture forhigh frequency plant regeneration of Maspine pineapple (Ananas comosus L.), eight different culturemedia formulation were evaluated for their effects on the induction of somatic embryos from suckerexplants. Explants were cultured on MS medium supplemented with various media concentration(NAA, Dicamba and BAP, Picloram, Kinetin and NAA, 2,4-D, TDZ, and TDZ and BAP).Embryogenic callus induction percentage, color and texture of the callus were assessed after fivemonths of culture. The optimum medium for the proliferation of in vitro shoots from sucker explantswas MS medium supplemented with 3 mg/L BAP. Meanwhile, the optimum medium for the inductionof fastest and high percentage of embryogenic callus growth from in vitro leaf-based was MS mediumsupplemented with Picloram. Results of mean comparison showed that 3 mg/L Picloram were moreeffective on explants than 10 mg/L. Results of the double staining method proved that somaticembryogenesis occurred in MS supplemented with 3 mg/L Picloram. Under microscopic observations,the globular-stage of the embryos were revealed in callus cells which is relatively suitable forsuspension cells inoculums, indicating that the tested PGR were significantly effective for somaticembryogenesis formation in this species. Most embryogenic callus from sucker explants wasyellowish-mucilaginous-wet-friable. The developed protocol potentially leads to the production ofembryogenic callus from sucker explants and plant regeneration through somatic embryogenesis.

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