scholarly journals Callus Induction and Elicitation of Total Phenolics in Callus Cell Suspension Culture of Celastrus paniculatus – willd, an Endangered Medicinal Plant in India

2016 ◽  
Vol 8 (5) ◽  
pp. 471-475 ◽  
Author(s):  
Anusha T S ◽  
Joseph M V ◽  
Elyas K K
Keyword(s):  
Medicinal Plant ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Total Phenolics ◽  
Callus Cell ◽  
Endangered Medicinal Plant ◽  
Celastrus Paniculatus

scholarly journals Factors Affecting the Selection of Callus Cell Lines and the Preparation of the Cell Suspension Culture of Artemisia annua L.

2014 ◽  
Vol 23 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Ch’ng Song Jin ◽  
Chan Lai Keng
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Lines ◽  
Cell Suspension Culture ◽  
Artemisia Annua ◽  
Leaf Explants ◽  
In Vitro Production ◽  
Callus Cell ◽  
Alternative Mode ◽  
Artemisia Annua L

Artemisinin, an important antimalarial drug against multidrug resistant strains of Plasmodium, can be produced in Artemisia annua L. Field production of artemisinin is affected by environmental condition and geographical location. In vitro production via cell suspension culture is an alternative mode and cell line selection is important to ensure sustainable production of biomass and artemisinin. Callus cell lines were derived from the leaf explants of five A. annua clones grown in two different locations in Vietnam. Thirty-four callus cell lines with consistent growth index (GI) were selected from these five clones and were categorized into fast (GI > 20), intermediate (GI 15 - 20) and slow (GI < 15) growing groups. The selected lines were found to have different morphology in term of colour and texture. The callus texture did affect the cell growth of A. annua in which the friable callus type showed faster, consistent and sustainable cell biomass production.D. O. I.http://dx.doi.org/10.3329/ptcb.v23i2.17507Plant Tissue Cult. & Biotech. 23(2): 157-163, 2013  (December)

scholarly journals Callus induction and cell culture of castor (Ricinus communis L. CV. Shabje)

2014 ◽  
Vol 20 ◽  
pp. 161-169
Author(s):  
MA Rahman ◽  
MA Bari
Keyword(s):  
Cell Culture ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Secondary Metabolite ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Ricinus Communis ◽  
Culture Technique ◽  
Culture Industry ◽  
Cell Culture Technique

Context: Tissue culture is an important tool in plant biotechnology that allows for an increase in biomass or metabolite production by utilizing several techniques in callus or cell cultures. Ricin is a toxic protein that can be extracted from the castor bean as secondary metabolite. The ricin has been used experimentally in medicine to kill cancer cell. We developed callus and cell culture technique for the possible extraction of ricin from the cell suspension culture of Ricinus communis. Objectives: The present investigation aimed to develop the cell culture technique of R. communis L. cv. Shabje and tried to establish a protocol for cell suspension culture of castor for possible extraction of ricin from cell extract. Materials and Methods: The hypocotyl explants of R. communis L. cv. Shabje were used as experimental materials. Cultured on Murashige and Skoog medium supplemented with different concentrations and combinations of BAP, NAA, 2,4-D and IAA for callus induction. For cell culture, the media were used without agar with different concentrations and combinations of these hormones. Results: For callus induction the combination of BAP 2.0 mg/l + 0.5 mg/l NAA showed the best performance but in case of cell culture the combination of BAP 2.0 mg/l and 0.2 mg/l NAA showed the best result. Conclusion: The present investigation clearly established and demonstrated the method of obtaining cell suspension culture and important secondary metabolite ricin could be obtained from cell suspension culture of R. communis L. holding promises to explore cell culture industry for ricin production. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17738 J. bio-sci.  20:  161-169, 2012

scholarly journals Sink ? Source System of In vitro Suspension Culture of Celastrus paniculatus under Regulation of Monochromatic Lights

2016 ◽  
Vol 26 (2) ◽  
pp. 175-185 ◽  
Author(s):  
Vandita Billore ◽  
Lalit Khatediya ◽  
Monica Jain
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Culture ◽  
Cell Mass ◽  
Scale Up ◽  
Light Conditions ◽  
Yellow Light ◽  
Celastrus Paniculatus

Plants are wonderful resource of bioproducts encompassing significant value to medicines and drug development. The plant cell suspension cultures bear immense potential for production of high?value secondary metabolites and are chosen as alternative sources of raw material for industrial use. In the present study, homogenous cell suspension culture of Celastrus paniculatus a medicinally important plant was established and multifold production of alkaloids and total phenols was obtained under the influence of monochromatic lights. One month old leaf derived friable callus of C. paniculatus was used to raise homogenous suspension culture and kept on rotary shakers in cabinets illuminated with different monochromatic LED lights (Blue, Yellow and Red). The monochromatic lights proved to be a strong abiotic elicitor in driving the production of secondary metabolites so much so that the metabolites were released extracellularly and the medium served as sink or spacious pool for leaked out metabolites from the cell mass. Maximum production and enhancement in alkaloids and phenols (98 and 44.7%, respectively) over control was obtained from cell mass grown under yellow light treatment, followed by blue (64 and 23.7%) and red light (50 and 26%) treatments. Further scale up of secondary metabolite production was hence performed under yellow light conditions, starting from 2.5 gm cell mass suspended in 250 ml of media extended up to 1000 ml culture media for one month. The continuous culture system exhibited remarkable potential of this plant cell system as multifold yield of total alkaloids (91.69 ?g/1750 ml) and phenols (70.59 ?g/1750 ml) was obtained during 30 days of culture under yellow light conditions. The production was remarkably enhanced by 100?folds (5.29 to 48.21 ?g; Fig. 2) for alkaloids and 70?folds (1.73 to 46.33 ?g; Fig. 3) for phenols, from zero days to 30?day?old culture phase. Hence, strategic implementation of monochromatic lights holds great promises for controlled commercial production of myriads of valuable secondary metabolites from plant cell cultures of important medicinal plants. Present work contributes to the first reports where continuous, enhanced, multifold yield of important secondary metabolites were obtained from cell suspension culture of Celasrus paniculatus an endangered medicinal plant.Plant Tissue Cult. & Biotech. 26(2): 175-185, 2016 (December)

scholarly journals Potential Embryogenic Callus Induction Protocol Through Cell Suspension Culture For High Frequency Plant Regeneration Of Maspine Pineapple (Ananas comosus L.)

1970 ◽  
Vol 3 (2) ◽  
pp. 40-45
Author(s):  
M.F. Mohamad Bukhori ◽  
Norzulaani Khalid ◽  
Ch'ng Lou Ven
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Embryogenic Callus Induction

To explore the potential for embryogenic callus induction protocol through cell suspension culture forhigh frequency plant regeneration of Maspine pineapple (Ananas comosus L.), eight different culturemedia formulation were evaluated for their effects on the induction of somatic embryos from suckerexplants. Explants were cultured on MS medium supplemented with various media concentration(NAA, Dicamba and BAP, Picloram, Kinetin and NAA, 2,4-D, TDZ, and TDZ and BAP).Embryogenic callus induction percentage, color and texture of the callus were assessed after fivemonths of culture. The optimum medium for the proliferation of in vitro shoots from sucker explantswas MS medium supplemented with 3 mg/L BAP. Meanwhile, the optimum medium for the inductionof fastest and high percentage of embryogenic callus growth from in vitro leaf-based was MS mediumsupplemented with Picloram. Results of mean comparison showed that 3 mg/L Picloram were moreeffective on explants than 10 mg/L. Results of the double staining method proved that somaticembryogenesis occurred in MS supplemented with 3 mg/L Picloram. Under microscopic observations,the globular-stage of the embryos were revealed in callus cells which is relatively suitable forsuspension cells inoculums, indicating that the tested PGR were significantly effective for somaticembryogenesis formation in this species. Most embryogenic callus from sucker explants wasyellowish-mucilaginous-wet-friable. The developed protocol potentially leads to the production ofembryogenic callus from sucker explants and plant regeneration through somatic embryogenesis.

scholarly journals DIFFERENTIAL EFFECT OF PURIFIED QUERCETIN AND ITS DERIVATIVES FROM IN VITRO CELL SUSPENSION CULTURES OF CAESALPINIA PULCHERRIMA SW. AGAINST SELECTED CANCER CELL LINES AND ITS MODE OF ACTION

2018 ◽  
Vol 8 (5-s) ◽  
pp. 196-208
Author(s):  
JM Aswathy ◽  
Greeshma Murukan ◽  
Bosco Lawarence ◽  
K Murugan
Keyword(s):  
Flow Cytometry ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Lines ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Mode Of Action ◽  
Cell Suspension Cultures ◽  
Mcf 7

Tribal people use the floral extract of Caesalpinia pulcherrima to cure liver, stomach and skin prone disorders in traditional Indian medicine. This study aimed to evaluate the effect of purified quercetin and its derivatives from in vitro cell suspension cultures of C. pulcherrima Sw. against SW 480, HeLa, MCF-7 and MCF 10A cell lines and its mode of action. Standard protocol was developed for callus induction using leaf explants. Cytotoxic effect was evaluated against SW 480, HeLa, MCF-7 and MCF 10A cells by MTT assay. Apoptosis was evaluated via Hoechst analysis,  flow cytometry, mitochondrial membrane potential and caspase 3 and 9 expression. 2, 4-D (2.5 mg/l), BAP (2.5 mg/l) + kin (1 mg/ml) was effective for remarkable callus induction. Further, cell suspension culture was established.  Effect of elicitors on cell suspension culture was also carried. Sucrose, ABA and salicylic acid (SA) at different concentrations influenced cell biomass and quercetin synthesis. Cells cultured on the medium fortified with 45 g/L sucrose without ABA/SA showed the highest quercetin content (16.5 mg/g). Quercetin was purified, fractionated by HPLC-DAD and was further analyzed by NMR revealed a major fraction of quercetin (3, 5, 7, 3’, 4’-pentahydroxyflavon). Insignificant cytotoxicity was noticed in SW 480, HeLa, MCF 10A when compared to MCF-7 cell lines exposed to different concentrations of purified quercetin for 24- 48 h. Similarly, the apoptosis by nuclei staining using Hoechst 33258 revealed a concentration dependent effect on MCF 7 cells only. This was further substantiated by caspase-9 and 3 induction and mitochondrial depolarization as revealed by flow cytometry. Overall, the results showed that quercetin and its derivatives induced effective apoptosis on MCF-7 cells. Quercetin isolated from the in vitro cell suspension culture of C. pulcherrima showed significant cytotoxicity and apoptotic activity towards MCF-7 cell lines as compared to other cell lines. Keywords:  Caesalpinia pulcherrima; quercetins; suspension culture; cytotoxicity; apoptotic.

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