scholarly journals Rooting of the Endemic Brazilian Species Campomanesia pubescens using Biotechnological Techniques

2022 ◽  
Vol 31 (2) ◽  
pp. 173-177
Author(s):  
NA Campos ◽  
GJ Da Silva ◽  
R Paiva

Abstract not available Plant Tissue Cult. & Biotech. 31(2): 173-177, 2021 (December)

Author(s):  
Russell L. Steere ◽  
Eric F. Erbe

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.


Author(s):  
R. D. Sjolund ◽  
C. Y. Shih

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.


Planta Medica ◽  
2013 ◽  
Vol 79 (05) ◽  
Author(s):  
M Mujeeb ◽  
M Amir ◽  
AS Nadeem ◽  
M Aqil ◽  
AK Najmi ◽  
...  

Biomics ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 387-399 ◽  
Author(s):  
S.V. Veselova ◽  
G.F. Burkhanova ◽  
T.V. Nuzhnaya ◽  
S.D. Rumyantsev ◽  
I.V. Maksimov

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  

2016 ◽  
Vol 47 (5) ◽  
pp. 630-638 ◽  
Author(s):  
Elena Mikhailova ◽  
Donald Hagan ◽  
Julia Sharp ◽  
Tristan Allerton ◽  
Kylie Burdette ◽  
...  

1931 ◽  
Vol 91 (1) ◽  
pp. 127-134
Author(s):  
John W. Crist ◽  
Marie Dye
Keyword(s):  

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