scholarly journals Tissue Specific Esterase Isozyme Banding Pattern in Nile Tilapia (Oreochromis niloticus)

1970 ◽  
Vol 27 ◽  
pp. 1-5 ◽  
Author(s):  
Reza Md Shahjahan ◽  
Afroja Karim ◽  
Rowshan Ara Begum ◽  
Mohammad Shamimul Alam ◽  
Anwara Begum
Keyword(s):  
Substrate Specificity ◽  
Banding Pattern ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Eye Lens ◽  
Relative Mobility ◽  
Esterase Isozyme ◽  
Hind Gut ◽  
Naphthyl Acetate ◽  
Different Tissues

The electrophoretic banding pattern of esterase isozymes were examined in eighteen different tissues of Nile tilapia after staining with α and β naphthyl acetate as substrate. The tissues were anterior-, mid-, tailmuscle (ventral and tip region), buccal muscle, stomach, fore-, mid-, hind-gut, liver, gill, heart, kidney, eye (lens), eye (iris), fore-, mid- and hind- brain. Altogether five bands named as Est-11.4, Est-21.1, Est-31, Est-40.62 and Est-50.25 were observed different relative mobility. Est-1 and Est-5 denote the fastest and slowest band. Est-3 was present in all the tissues. All five bands were expressed in liver and stomach. Some of the esterase bands showed tissue and substrate specificity, where Est-1 was in fore-, mid- and hind- brain, Est-2 in hind gut, Est-5 in stomach, gill and heart was stained only with α naphthyl acetate. None of the band was expressed with β naphthyl acetate only. Key words: esterase, isozyme, Oreochromis, tissue   Doi:10.3329/ujzru.v27i0.1942 Univ. j. zool. Rajshahi Univ. Vol. 27, 2008 pp. 01-05

scholarly journals Comparison of Esterase Isozyme Variability in Some Selected Tissues of the Asian and African Catfishes (Siluriformes: Clariidae)

2012 ◽  
Vol 40 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Rowshan Ara Begum ◽  
Dilshad Tamanna Rahman ◽  
Md Abdul Rashid ◽  
Md Shamimul Alam ◽  
Reza Md Shahjahan
Keyword(s):  
Allelic Variation ◽  
Eye Lens ◽  
African Catfish ◽  
Esterase Isozyme ◽  
Ventral Region ◽  
Specific Localization ◽  
Isozyme Variability ◽  
Asian Catfish ◽  
Tail Muscle ◽  
Different Tissues

Variation in esterase isozymes expression of 19 different tissues of the Asian catfish (Clarias batrachus) and African catfish (C. gariepinus) was studied. These tissues were: liver, anterior muscle, mid muscle, tail muscle (ventral region), tail muscle (tip region), buccal muscle, stomach, foregut, midgut, hindgut, kidney, gill, heart, eye (lens), eye (black portion), pelvic muscle, fore-, mid- and hind-brain. Maximum five esterase bands, viz. Est-11.83, Est-21.50, Est-31.15, Est-41.00 and Est- 50.17 were observed in the Asian catfish and four bands, viz. Est-21.50, Est-31.15, Est-41.00 and Est-50.17 in the African catfishes. Tissue specific localization of the isozymes was observed in both Asian and African catfishes. Comparatively higher esterase activity was found in digestive tissues. Higher number of esterase bands was found in C. batrachus which seems to be an indication of its greater allelic variation in esterases than those in the C. gariepinus. DOI: http://dx.doi.org/10.3329/bjz.v40i1.12893 Bangladesh J. Zool. 40(1): 43-50, 2012

scholarly journals Bioaccumulation of heavy metals in different tissues of Nile tilapia (Oreochromis niloticus) in Bangladesh

2019 ◽  
Vol 25 (2) ◽  
pp. 237-246
Author(s):  
Afroza Parvin ◽  
◽  
Md Kamal Hossain ◽  
Shabiha Islam ◽  
Saborni Swarna Das ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Different Tissues

scholarly journals Electrophoretic Banding Pattern Of Esterase Isozyme In Different Tissues Of Puntius Sophore (Cyprinidae : Cypriniformes)

2016 ◽  
Vol 42 (2) ◽  
pp. 201-208
Author(s):  
Hawa Jahan ◽  
Partha Sarathi Gope ◽  
Mohammad Shamimul Alam ◽  
Reza Md Shahjahan
Keyword(s):  
Skeletal Muscles ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Reproductive Organ ◽  
Local Market ◽  
Esterase Isozyme ◽  
Single Allele ◽  
Hind Gut ◽  
Different Tissues ◽  
Puntius Sophore

Esterase isozyme of different tissues of Puntius sophore was analyzed using 7.5 % polyacrylamide gel electrophoresis (PAGE). Fish specimens were collected from local market. The tissues used were taken from different muscles, stomach, fore-, mid- and hind-gut, liver, eyes, gill, heart, kidney, fore-, mid- and hind- brain, eggs and ovary. Six different esterase bands were detected, named Est-1, Est-2, Est-3, Est-4, Est-5 and Est-6 and their relative mobility were 1.0, 0.84, 0.62, 0.33, 0.26 and 0.13 respectively, each of them representing a single allele. The highest esterase activity was found in liver, followed by gill, kidney, heart, brain, intestine, stomach, eye, reproductive organ and skeletal muscles as detected in the staining intensity. Staining intensity of Est-4 and Est-5 was higher and Est-6 was the least stained in all the tissues. Asiat. Soc. Bangladesh, Sci. 42(2): 201-208, December 2016

The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

2014 ◽  
pp. 27
Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Bacterial Disease ◽  
Immersion Method ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Different Ages ◽  
Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

Incidence and molecular characterization of fungi and yeast isolated from cultured catfish and Nile tilapia

2020 ◽  
Vol 21 (3) ◽  
pp. 61-66
Author(s):  
Ola Hashem ◽  
Viola Zaki ◽  
Rawia Adawy
Keyword(s):  
Molecular Characterization ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Clarias Gariepinus ◽  
Fungal Species ◽  
High Rate ◽  
Fish Farms ◽  
Isolation And Identification ◽  
Penicillium Spp

Objective: To study the incidence and seasonal dynamics of different fungi affected freshwater fishes in Lake Manzala with molecular identification of the isolated fungi. Animals: 300 Nile tilapia (Oreochromis niloticus) and 300 catfish (Clarias gariepinus). Design: Descriptive study. Procedures: Random samples of Oreochromis niloticus (O. niloticus) and Clarias gariepinus (C. gariepinus) were collected from Manzala fish farms. Clinical and postmortem examination of fish was applied. Isolation and identification of different fungi were performed by conventional methods. Furthermore, the molecular characterization of isolated fungi was carried out. Results: C. gariepinus had a higher rate of infection with different fungal species than O. niloticus. Aspergillus spp. (Aspergillus niger and Aspergillus flavus) were the most fungal isolated from the examined fishes, followed by Penicillium spp. and Candida albicans. Aspergillus spp were detected in all seasons with a higher rate in summer and spring. A. flavus, A. niger, Penicillium spp. and C.albicans isolates were amplified from both C. gariepinus and O. niloticus at the specified molecular weight using PCR. Conclusion and clinical relevance: Fungal infection affected the fish showing different external and internal lesions, all species of Aspergillus were found in all seasons with a high rate in, hot seasons, summer and spring. The Prevalence of Penicillium and C. albicans were also reported. All fungal isolates were identified on the phenotypic and molecular bases.

Effect of low temperature on genomic DNA methylation in Nile tilapia (Oreochromis niloticus)

2013 ◽  
Vol 37 (10) ◽  
pp. 1460 ◽  
Author(s):  
Huaping ZHU ◽  
Maixin LU ◽  
Zhanghan HUANG ◽  
Fengying GAO ◽  
Xiaoli KE ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Low Temperature ◽  
Oreochromis Niloticus ◽  
Genomic Dna ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus
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