scholarly journals Electrophoretic Banding Pattern Of Esterase Isozyme In Different Tissues Of Puntius Sophore (Cyprinidae : Cypriniformes)

2016 ◽  
Vol 42 (2) ◽  
pp. 201-208
Author(s):  
Hawa Jahan ◽  
Partha Sarathi Gope ◽  
Mohammad Shamimul Alam ◽  
Reza Md Shahjahan
Keyword(s):  
Skeletal Muscles ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Reproductive Organ ◽  
Local Market ◽  
Esterase Isozyme ◽  
Single Allele ◽  
Hind Gut ◽  
Different Tissues ◽  
Puntius Sophore

Esterase isozyme of different tissues of Puntius sophore was analyzed using 7.5 % polyacrylamide gel electrophoresis (PAGE). Fish specimens were collected from local market. The tissues used were taken from different muscles, stomach, fore-, mid- and hind-gut, liver, eyes, gill, heart, kidney, fore-, mid- and hind- brain, eggs and ovary. Six different esterase bands were detected, named Est-1, Est-2, Est-3, Est-4, Est-5 and Est-6 and their relative mobility were 1.0, 0.84, 0.62, 0.33, 0.26 and 0.13 respectively, each of them representing a single allele. The highest esterase activity was found in liver, followed by gill, kidney, heart, brain, intestine, stomach, eye, reproductive organ and skeletal muscles as detected in the staining intensity. Staining intensity of Est-4 and Est-5 was higher and Est-6 was the least stained in all the tissues. Asiat. Soc. Bangladesh, Sci. 42(2): 201-208, December 2016

scholarly journals Tissue Specific Esterase Isozyme Banding Pattern in Nile Tilapia (Oreochromis niloticus)

1970 ◽  
Vol 27 ◽  
pp. 1-5 ◽  
Author(s):  
Reza Md Shahjahan ◽  
Afroja Karim ◽  
Rowshan Ara Begum ◽  
Mohammad Shamimul Alam ◽  
Anwara Begum
Keyword(s):  
Substrate Specificity ◽  
Banding Pattern ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Eye Lens ◽  
Relative Mobility ◽  
Esterase Isozyme ◽  
Hind Gut ◽  
Naphthyl Acetate ◽  
Different Tissues

The electrophoretic banding pattern of esterase isozymes were examined in eighteen different tissues of Nile tilapia after staining with α and β naphthyl acetate as substrate. The tissues were anterior-, mid-, tailmuscle (ventral and tip region), buccal muscle, stomach, fore-, mid-, hind-gut, liver, gill, heart, kidney, eye (lens), eye (iris), fore-, mid- and hind- brain. Altogether five bands named as Est-11.4, Est-21.1, Est-31, Est-40.62 and Est-50.25 were observed different relative mobility. Est-1 and Est-5 denote the fastest and slowest band. Est-3 was present in all the tissues. All five bands were expressed in liver and stomach. Some of the esterase bands showed tissue and substrate specificity, where Est-1 was in fore-, mid- and hind- brain, Est-2 in hind gut, Est-5 in stomach, gill and heart was stained only with α naphthyl acetate. None of the band was expressed with β naphthyl acetate only. Key words: esterase, isozyme, Oreochromis, tissue   Doi:10.3329/ujzru.v27i0.1942 Univ. j. zool. Rajshahi Univ. Vol. 27, 2008 pp. 01-05

Inheritance of aspartate aminotransferase (AAT) in Cucumis species as revealed by interspecific hybridization

2006 ◽  
Vol 84 (9) ◽  
pp. 1503-1507 ◽  
Author(s):  
Jin-Feng Chen ◽  
Gang Ren ◽  
Xiang-Dong Luo ◽  
Jack Staub ◽  
Molly M. Jahn
Keyword(s):  
Cucumis Sativus ◽  
Aspartate Aminotransferase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Recurrent Parent ◽  
Cucumis Sativus L ◽  
Dosage Effects ◽  
Cucumis Species ◽  
Single Allele ◽  
The Difference

The inheritance of aspartate aminotransferase (AAT) isozymes was investigated in Cucumis sativus L. (CC; 2n = 2x = 14), Cucumis hystrix Chakr. (HH; 2n = 2x = 24), the synthetic amphidiploid species C. hytivus Chen & Kirkbride (HHCC; 2n = 4x = 38), and the allotriploid (HCC; 2n = 3x = 26) from backcrossing C. hytivus to C. sativus. Two polymorphic loci, Aat-1 and Aat-2, and one monomorphic locus, Aat-3, were detected among these parents and their progenies by using polyacrylamide gel electrophoresis. Cucumis sativus displayed the fast-migrating anodic band for Aat-1 (2), while C. hystrix contained a slow-migrating cathodic band (1). For Aat-2, the slow-migrating cathodic band was observed with C. sativus (1), whereas C. hystrix contained the fast-migrating anodic band (2). Cucumis hytivus, a synthetic species derived from doubling the chromosome number of a F1 from a C. sativus × C. hystrix mating, exhibited the typical hybrid dimeric banding pattern resulting from the combination of two parental homomeric products with equal staining intensity and a heteromeric product with intermediate mobility and greater staining intensity than either homomeric product. The difference in band intensity between C. hytivus and its backcross progenies, when C. sativus was the recurrent parent, were due to the dosage effects of alleles at Aat-1 and Aat-2. These banding morphotypes can be used for typing of C. hytivus and BC1 progenies that are similar in morphology. Aat-3 was monomorphic in this mating, encoding a single allele Aat-3 (1).

scholarly journals Esterase variability in different tissues of Naked Neck Fowl (Gallus gallus domesticus) of Bangladesh using polyacrylamide gel electrophoresis

1970 ◽  
Vol 8 (1) ◽  
pp. 51-55
Author(s):  
QS Akter ◽  
KA Muid ◽  
KMA Tareq ◽  
MAMY Khandoker
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Polyacrylamide Gel ◽  
Gallus Gallus Domesticus ◽  
Esterase Isozyme ◽  
Indigenous Chicken ◽  
Naked Neck ◽  
Different Tissues

Naked neck fowl (Gallus gallus domesticus) comprises one of the indigenous chicken populations in Bangladesh. The present investigation was conducted to study the polymorphic pattern of the estrase isozyme in different tissues of the fowl by polyacrylamide gel electrophoresis (PAGE) system. In this experiment, α-napthyle and β-napthyle acetate were used as substrate. Esterase variability of different samples of fore brain, mid brain, hind brain, heart brain, liver, testis, oesophagus, gizzard, bile ducts, proventiculus, small intestine, large intestine pectoral muscle, pelvic muscle, lung, eye, kidney, pancreas, body muscle and blood cells were examined. Altogether four esterase bands were observed and the bands were designated as Esterase Est-1 2.0, Est-21.0 Est-30.75 and Est-40.15. Among the four bands Est-1 was the fastest and placed near to anode (+) electrode. Esterase showed tissue specific variation. Est-2 was found in almost all tissues, whereas Est-1 was observed only in liver. Est-3 and Est-4 were expressed exclusively in the nervous system especially in brain. Such findings may provide basic information to analyze the esterase activity in different tissues. Keywords: Esterase; Isozyme; Naked neck fowl; Tissue DOI: 10.3329/jbau.v8i1.6398J. Bangladesh Agril. Univ. 8(1): 51-55, 2010

Detection of Protein Changes in Serum of Workers following Inhalation Exposure to Toluene Diisocyanate Vapors

1992 ◽  
Vol 8 (6) ◽  
pp. 407-413 ◽  
Author(s):  
Adam B. Czuppon ◽  
Boleslaw Marczynski ◽  
Xaver Baur
Keyword(s):  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Inhalation Exposure ◽  
Staining Intensity ◽  
Peak Height ◽  
Toluene Diisocyanate ◽  
Serum Samples ◽  
Chi Square ◽  
Challenge Tests

Serum samples of 10 workers undergoing occupational type inhalative challenge tests by toluene diisocyanate (TDI) were investigated by anion-exchange fast-protein-liquid-chromatography (FPLC) and polyacrylamide-gel electrophoresis (PAGE-SDS). Their serum chromatography profiles were compared to those of 20 unexposed individuals. The peak height of the first prealbumin peak in sera of workers after inhalative challenge tests was significantly different (p > 0, 01 Chi-square test) compared to that obtained before exposure and to that of unexposed subjects. In addition, qualitative changes of these peaks were also noted in sera of workers exposed to TDI. In the cases of exposed individuals, that peak was more diffuse with some shoulders and less symmetric in appearance. Similarly, PAGE-SDS of the serum proteins, followed by silver nitrate staining, revealed a different banding pattern after in vivo TDI exposure. One of the serum components at approximately 15 kD showed an increase of staining intensity after exposure (n = 10), compared to unexposed subjects or to patients before exposure. This serum fraction has not yet been identified. The results here demonstrate that it is possible to detect changes of serum proteins in TDI-exposed individuals within a relatively short analysis time. This could be useful for biological monitoring of exposure, since no method for such is yet available.

A biochemical and toxicological study of the role of insensitive acetylcholinesterase in organophosphorus resistantBemisia tabaci(Homoptera: Aleyrodidae) from Israel

1994 ◽  
Vol 84 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Frank J. Byrne ◽  
Matthew Cahill ◽  
Ian Denholm ◽  
Alan L. Devonshire
Keyword(s):  
Bemisia Tabaci ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Female Offspring ◽  
Polyacrylamide Gel ◽  
Microplate Assay ◽  
Toxicological Study ◽  
Single Insect

AbstractTwo acetylcholinesterase (AChE) variants, differing in sensitivity to inhibition by the organophosphorus (OP) insecticide paraoxon were identified in a population ofBemisia tabaci(Gennadius) from cotton in Israel using a single insect kinetic microplate assay. Two strains were established, homogeneous for one or other of the two variants, by isolating mated females from the field population onto individual cotton leaves, and testing a proportion of their female offspring to identify their AChE genotype. Polyacrylamide gel electrophoresis of their I-naphthyl butyrate hydrolyzing esterases showed that all insects contained esterase E0 14, which is indicative of B-type whiteflies, although the staining intensity of this band differed. Resistance to the OPs monocrotophos, profenofos and chlorpyrifos in leaf dip bioassays was consistent with the presence of the insensitive AChE. The data also indicated that separate mechanisms conferred resistance to the two pyrethroids cypermethrin and bifenthrin. The former, when used in a mixture with profenofos, was no more toxic than when the OP was used alone, and resistance to the mixture was largely dependent on the presence of the insensitive AChE.

scholarly journals Preferential cross-linking of the small subunit of the electron-transfer flavoprotein to general acyl-CoA dehydrogenase

1987 ◽  
Vol 243 (2) ◽  
pp. 519-524 ◽  
Author(s):  
D J Steenkamp
Keyword(s):  
Electron Transfer ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Small Subunit ◽  
Cross Linking ◽  
Thiol Groups ◽  
Electron Transfer Flavoprotein ◽  
Acceptor Activity ◽  
Lysine Residues

The interaction between pig liver mitochondrial electron-transfer flavoprotein (ETF) and general acyl-CoA dehydrogenase (GAD) was investigated by means of the heterobifunctional reagent N-succinimidyl 3-(2-pyridyldithio)propionate. Neither ETF or GAD contained reactive thiol groups. The substitution of 9.4 lysine residues/FAD group in GAD with pyridyl disulphide structures did not affect the catalytic activity of the enzyme. Thiol groups were introduced into ETF by thiolation with methyl 4-mercaptobutyrimidate. ETF containing 10.5 reactive thiol groups/FAD group showed undiminished electron-acceptor activity with respect to GAD. The reaction of thiolated ETF and GAD containing pyridyl disulphide structures resulted in a decreased staining intensity of the small subunit of ETF on SDS/polyacrylamide-gel electrophoresis. Preferential cross-linking of the smaller subunit of ETF to GAD did not take place when ETF was first treated with SDS, but was unaffected by reduction of GAD by octanoyl-CoA.

scholarly journals Up-regulation of Per1 expression by estradiol and progesterone in the rat uterus

2007 ◽  
Vol 194 (3) ◽  
pp. 511-519 ◽  
Author(s):  
Pei-Jian He ◽  
Masami Hirata ◽  
Nobuhiko Yamauchi ◽  
Masa-aki Hattori
Keyword(s):  
Stromal Cells ◽  
Clock Genes ◽  
Staining Intensity ◽  
Reproductive Organ ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Rat Uterus ◽  
Ovarian Steroids ◽  
Direct Regulation

It has been established that estrogen can alter circadian rhythms in behavior and endocrine physiology in rodents. The uterus is a reproductive organ that is critically dependent on regulation by ovarian steroids. Here, we examined the expression of Per1 in different compartments of the uterus, and explored whether the ovarian steroids could regulate Per1 expression employing ovariectomized rat uterus. RT-PCR analysis showed that Per1 was cyclically expressed in the uterus. As revealed by in situ hybridization, the staining intensity of Per1 mRNA was stronger at ZT 8 than at ZT 0 in the uterine luminal epithelium (LE), stroma (S), and myometrium (M) compartments, but was not changed in the glandular epithelium (GE). Both in situ hybridization and immunofluorescence analyses revealed that estradiol (E2) administration induced high expression of Per1 in the LE, GE, and M, and less expression in the S compartment. Progesterone (P4) treatment resulted in an obvious enhancement of Per1 expression in the LE, GE, and S, but unchanged in the M compartment. Furthermore, the E2- and P4-activated Per1 expression was significantly repressed by their respective antagonists, ICI182 780 and RU486. These findings were further supported by RT-PCR analysis of Per1 expression in cultured uterine stromal cells. Collectively, the present data indicate that E2 and P4 might be involved in modification of circadian rhythm via direct regulation of the expression of clock genes.

scholarly journals The small dermatan sulphate proteoglycans synthesized by fibroblasts derived from skin, synovium and gingiva show tissue-related heterogeneity

1988 ◽  
Vol 256 (1) ◽  
pp. 35-40 ◽  
Author(s):  
H Larjava ◽  
J Heino ◽  
T Krusius ◽  
E Vuorio ◽  
M Tammi
Keyword(s):  
Extracellular Matrix ◽  
Molecular Mass ◽  
Gel Electrophoresis ◽  
Core Protein ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Gingival Fibroblasts ◽  
Dermatan Sulphate ◽  
Different Tissues

Dermatan sulphate proteoglycans (DSPGs) synthesized in the presence of 35SO4 were characterized in culture media of fibroblast lines obtained from skin, synovium, and gingiva. The molecular mass of DSPG varied from 95-130 kDa as estimated by SDS/polyacrylamide-gel electrophoresis. Gingival fibroblasts constantly produced larger DSPGs than skin fibroblasts. This was due to the larger dermatan sulphate (DS) chains, which also showed tissue-related heterogeneity in the distribution of 4- and 6-sulphated disaccharide units. The N-glycosylated cores (44 and 47 kDa) obtained following chondroitinase ABC treatment were of identical size in all tissues. The cores from the different tissues were also of the same size (38 kDa) when addition of the N-linked oligosaccharides was inhibited by tunicamycin or when they were removed by N-glycanase treatment. No evidence for low-molecular-mass sulphated oligosaccharides was found. All tissues contained two mRNA species (1.6 and 1.9 kb) for the DSPG core protein. These data suggest that the pattern of transferase activities involved in the construction of DS chains differs from one tissue to another. This variation may modulate the functions of DSPG in the extracellular matrix.

scholarly journals Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

2018 ◽  
Vol 19 (5) ◽  
pp. 1683-1689 ◽  
Author(s):  
NUR RAHMAH HIDAYATI ◽  
SURANTO SURANTO ◽  
SAJIDAN SAJIDAN
Keyword(s):  
Banding Pattern ◽  
Polyacrylamide Gel Electrophoresis ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Retardation Factor ◽  
Cucurbita Moschata ◽  
Esterase Isozyme ◽  
Banding Patterns ◽  
Different Altitudes

Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.

Effects of jinggangmycin on peroxidase and esterase isozymes and phytotoxin from Rhizoctonia solani AG-1 IA, the causal agent of rice sheath blight. Pirinç kabuğu yanığı nedeni Rhizoctonia solani AG-1 IA tarafından üretilen peroksidaz, esteraz izozimleri ve fitotoksinler üzerinde jinggangmycin etkisi

2016 ◽  
Vol 41 (4) ◽  
Author(s):  
Ying-Qing Yang ◽  
Ming-Hai Li ◽  
Mei Yang ◽  
Can-Wei Shu ◽  
Xiang-Min Li ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sheath Blight ◽  
Rice Sheath Blight ◽  
Esterase Isozyme ◽  
Esterase Isozymes ◽  
Peroxidase Isozyme ◽  
Rice Tissues

AbstractObjective: To explore the effects of jinggangmycin on the pathogenicity of rice sheath blight.Methods: The effects of jinggangmycin on peroxidase and esterase isozymes from Rhizoctonia solani Kuhn AG-1 IA were compared using non-denaturing polyacrylamide gel electrophoresis (PAGE). Rs-toxins were obtained from the improved Richard medium amended with jinggangmycin at different concentrations. Subsequently, bioassays of Rs-toxin, including virulence to rice tissues, seedling wilting and inhibition to the seed germination were conducted.Results: The results of PAGE showed that when the jinggangmycin concentration increased to 50 μg/mL, two of peroxidase isozyme bands became apparently weaker compared with the controls, indicating that jinggangmycin could considerably weaken the activity of the peroxidase isozyme. In addition, three extra bands of esterase isozyme were induced by jinggangmycin when the concentration increased to 50 μg/mL, indicating that jinggangmycin could affect the metabolism of esterase isozyme. The bioassays of Rs-toxin showed that jinggangmycin could weaken the virulence of Rs-toxin in rice tissues.Conclusion: It was concluded that the control role of jinggangmycin in rice sheath blight might be due to the effects of jinggangmycin on the peroxidase and esterase isozymes and Rs-toxin virulence.

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