scholarly journals Comparison of Esterase Isozyme Variability in Some Selected Tissues of the Asian and African Catfishes (Siluriformes: Clariidae)

2012 ◽  
Vol 40 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Rowshan Ara Begum ◽  
Dilshad Tamanna Rahman ◽  
Md Abdul Rashid ◽  
Md Shamimul Alam ◽  
Reza Md Shahjahan
Keyword(s):  
Allelic Variation ◽  
Eye Lens ◽  
African Catfish ◽  
Esterase Isozyme ◽  
Ventral Region ◽  
Specific Localization ◽  
Isozyme Variability ◽  
Asian Catfish ◽  
Tail Muscle ◽  
Different Tissues

Variation in esterase isozymes expression of 19 different tissues of the Asian catfish (Clarias batrachus) and African catfish (C. gariepinus) was studied. These tissues were: liver, anterior muscle, mid muscle, tail muscle (ventral region), tail muscle (tip region), buccal muscle, stomach, foregut, midgut, hindgut, kidney, gill, heart, eye (lens), eye (black portion), pelvic muscle, fore-, mid- and hind-brain. Maximum five esterase bands, viz. Est-11.83, Est-21.50, Est-31.15, Est-41.00 and Est- 50.17 were observed in the Asian catfish and four bands, viz. Est-21.50, Est-31.15, Est-41.00 and Est-50.17 in the African catfishes. Tissue specific localization of the isozymes was observed in both Asian and African catfishes. Comparatively higher esterase activity was found in digestive tissues. Higher number of esterase bands was found in C. batrachus which seems to be an indication of its greater allelic variation in esterases than those in the C. gariepinus. DOI: http://dx.doi.org/10.3329/bjz.v40i1.12893 Bangladesh J. Zool. 40(1): 43-50, 2012

scholarly journals Tissue Specific Esterase Isozyme Banding Pattern in Nile Tilapia (Oreochromis niloticus)

1970 ◽  
Vol 27 ◽  
pp. 1-5 ◽  
Author(s):  
Reza Md Shahjahan ◽  
Afroja Karim ◽  
Rowshan Ara Begum ◽  
Mohammad Shamimul Alam ◽  
Anwara Begum
Keyword(s):  
Substrate Specificity ◽  
Banding Pattern ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Eye Lens ◽  
Relative Mobility ◽  
Esterase Isozyme ◽  
Hind Gut ◽  
Naphthyl Acetate ◽  
Different Tissues

The electrophoretic banding pattern of esterase isozymes were examined in eighteen different tissues of Nile tilapia after staining with α and β naphthyl acetate as substrate. The tissues were anterior-, mid-, tailmuscle (ventral and tip region), buccal muscle, stomach, fore-, mid-, hind-gut, liver, gill, heart, kidney, eye (lens), eye (iris), fore-, mid- and hind- brain. Altogether five bands named as Est-11.4, Est-21.1, Est-31, Est-40.62 and Est-50.25 were observed different relative mobility. Est-1 and Est-5 denote the fastest and slowest band. Est-3 was present in all the tissues. All five bands were expressed in liver and stomach. Some of the esterase bands showed tissue and substrate specificity, where Est-1 was in fore-, mid- and hind- brain, Est-2 in hind gut, Est-5 in stomach, gill and heart was stained only with α naphthyl acetate. None of the band was expressed with β naphthyl acetate only. Key words: esterase, isozyme, Oreochromis, tissue   Doi:10.3329/ujzru.v27i0.1942 Univ. j. zool. Rajshahi Univ. Vol. 27, 2008 pp. 01-05

scholarly journals First report on the successful hybridization ofPangasianodon hypophthalmus(Sauvage, 1878) andClarias gariepinus(Burchell, 1822)

Zygote ◽  
2017 ◽  
Vol 25 (4) ◽  
pp. 443-452 ◽  
Author(s):  
V.T. Okomoda ◽  
I.C.C. Koh ◽  
M.S. Shahreza
Keyword(s):  
Clarias Gariepinus ◽  
The Other ◽  
Larval Performance ◽  
African Catfish ◽  
Parent Species ◽  
Morphological Comparison ◽  
Reciprocal Crosses ◽  
Pangasianodon Hypophthalmus ◽  
Artificial Fertilization ◽  
Asian Catfish

SummaryBreeding and larval performance of novel hybrids from reciprocal crosses of Asian catfishPangasianodon hypophthalmus(Sauvage, 1878) and African catfishClarias gariepinus(Burchell, 1822) were investigated in this study. Spawning was by hormonal injection of brood fish, artificial fertilization, and incubation in triplicate aquarium tanks (0.5 × 0.5 × 0.5 m3) with continuous aeration. Reciprocal crosses (♀C. gariepinus× ♂P. hypophthalmusand ♀P. hypophthalmus× ♂C. gariepinus) had lower hatchability (≤50%) than their pure siblings (≥75%). Fish from all crosses survived until the juvenile stage but survival at 35 days post hatching (dph) was higher for pureC. gariepinussib. ♀C. gariepinus× ♂P. hypophthalmuswas observed to be less resistant to degradation of water quality than the other crosses, however it had higher body weight compared with the other crosses that showed similar performance. Morphological comparison of surviving juvenile at 35 dph, showed that all ♀P. hypophthalmus× ♂C. gariepinusand 13% of the ♀C. gariepinus× ♂P. hypophthalmusexhibited the very same morphology as that of their maternal parent species, while the other portion of the ♀C. gariepinus× ♂P. hypophthalmuscross exhibited morphological traits that were intermediate between those of both parent species. This study been the first successful attempt to hybridize both species and therefore, laid the groundwork for further studies on the aquaculture potentials of the novel hybrids.

scholarly journals Tissue distribution of esterase isozymes and their responses to cypermethrin in three macrobrachium species

2013 ◽  
Vol 38 (2) ◽  
pp. 227-235
Author(s):  
Md Abdur Rashid ◽  
Rowshan Ara Begum ◽  
Reza Md Shahzahan
Keyword(s):  
Esterase Activity ◽  
Nerve Cord ◽  
Staining Intensity ◽  
Esterase Isozyme ◽  
Eye Muscle ◽  
Esterase Isozymes ◽  
Agricultural Pesticides ◽  
Isozyme Variability ◽  
Species Specific ◽  
The Impact

In recent times prawn grounds are getting contaminated with an increased use of agricultural pesticides. Esterases play an intermediary role in conferring or in contributing to insecticide resistance. Hence, Esterase isozyme variability was studied in three Macrobrachium species (M. rosenbergii, M. malcolmsonii and M. lamarrei) on 7.5% PAGE with ? and ? naphthyl acetate as substrate in terms of number of bands, staining intensity and toxicity effects. Expression of esterase isozymes was studied in five different tissues (eye, muscle, nerve cord, stomach and hepatopancreas) where four esterase bands (Est-1, Est-2, Est-3 and Est-4) were observed in M. rosenbergii and M. lamarrei and two bands (Est-1 and Est-4) in M. malcolmsonii. Bands in the hepatopancreas of M. rosenbergii, in the eye of M. lamarrei and in the nerve cord of M. malcolmsonii were highly intense indicating higher esterase activity. It was also noticed that the slowest bands with higher molecular weight were more expressed than the others and spreading area of a particular band was not fixed in all the tissues. To observe the impact of insecticide on esterases all five tissues were exposed to 1000 ppm of cypermethrin for 24 hours and was subjected to PAGE, it was found that treated samples showed less esterase expression. Only the muscle tissues from three species were exposed to a range of doses (0.078-1000 ppm), species specific fluctuation of esterase activity was observed in an irregular fashion. Bioassay conducted with cypermethrin on M. lamarrei indicated that LC50 was 0.05ppm at 2 hours. Fluctuation of esterase activity was observed with time and dose concentrations in post mortal electrophoretic assay. DOI: http://dx.doi.org/10.3329/jasbs.v38i2.15614 J. Asiat. Soc. Bangladesh, Sci. 38(2): 227-235, December 2012

scholarly journals Morphological, anatomical and isozyme variability among taro (Colocasia esculenta) accessions from southeastern part of Central Java, Indonesia

2018 ◽  
Vol 19 (5) ◽  
pp. 1811-1819 ◽  
Author(s):  
ARI PITOYO ◽  
ANGELIA ARUM PRAMETA ◽  
MARSUSI MARSUSI ◽  
SURATMAN SURATMAN ◽  
SURANTO SURANTO
Keyword(s):  
Banding Pattern ◽  
Morphological Characters ◽  
Colocasia Esculenta ◽  
Esterase Isozyme ◽  
Southeastern Part ◽  
Central Java ◽  
Wide Range ◽  
Significant Difference ◽  
Isozyme Variability ◽  
Anatomical Characters

Pitoyo A, Prameta AA, Marsusi, Suratman, Suranto. 2018. Morphological, anatomical and isozyme variability among taro (Colocasia esculenta) accessions from southeastern part of Central Java, Indonesia. Biodiversitas 19: 1811-1819. The objective of this study was to evaluate morphological, anatomical and isozyme variability among taro accessions from southeastern part of Central Java (Indonesia). A total of 20 taro accessions were collected from a wide range of sites during field surveys. Morphological characters measurements were taken on vegetative structures such as roots, stems, leaves, and corms. Anatomical characters were observed from both paradermal and transverse sections of leaf. Identification of biochemical markers was done by using peroxidase and esterase isozyme system. A UPGMA dendrogram among accessions was constructed based on the genetic similarity matrix by applying a cluster analysis using a computer programme, NTSYS Version 2.00. The analysis of variance for morphological and anatomical characters revealed that there was significant difference for majority of the tested traits indicating that there was a variability among the taro accessions. Polymorphism was observed using isozymes of esterase (12 banding pattern) and peroxidase (8 banding pattern). Based on the dendrogram at a level of 62 % similarity, taro accessions were segregated into two major clusters. In Cluster I, the closest relationship was shown between SKH and SKA accessions that had 96 % coefficient of similarity. The ten accessions from Klaten, Sragen, and Karanganyar were then clustered separately as Cluster II with coefficient of similarity 73.52 %.

scholarly journals Esterase variability in different tissues of Naked Neck Fowl (Gallus gallus domesticus) of Bangladesh using polyacrylamide gel electrophoresis

1970 ◽  
Vol 8 (1) ◽  
pp. 51-55
Author(s):  
QS Akter ◽  
KA Muid ◽  
KMA Tareq ◽  
MAMY Khandoker
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Polyacrylamide Gel ◽  
Gallus Gallus Domesticus ◽  
Esterase Isozyme ◽  
Indigenous Chicken ◽  
Naked Neck ◽  
Different Tissues

Naked neck fowl (Gallus gallus domesticus) comprises one of the indigenous chicken populations in Bangladesh. The present investigation was conducted to study the polymorphic pattern of the estrase isozyme in different tissues of the fowl by polyacrylamide gel electrophoresis (PAGE) system. In this experiment, α-napthyle and β-napthyle acetate were used as substrate. Esterase variability of different samples of fore brain, mid brain, hind brain, heart brain, liver, testis, oesophagus, gizzard, bile ducts, proventiculus, small intestine, large intestine pectoral muscle, pelvic muscle, lung, eye, kidney, pancreas, body muscle and blood cells were examined. Altogether four esterase bands were observed and the bands were designated as Esterase Est-1 2.0, Est-21.0 Est-30.75 and Est-40.15. Among the four bands Est-1 was the fastest and placed near to anode (+) electrode. Esterase showed tissue specific variation. Est-2 was found in almost all tissues, whereas Est-1 was observed only in liver. Est-3 and Est-4 were expressed exclusively in the nervous system especially in brain. Such findings may provide basic information to analyze the esterase activity in different tissues. Keywords: Esterase; Isozyme; Naked neck fowl; Tissue DOI: 10.3329/jbau.v8i1.6398J. Bangladesh Agril. Univ. 8(1): 51-55, 2010

scholarly journals Electrophoretic Banding Pattern Of Esterase Isozyme In Different Tissues Of Puntius Sophore (Cyprinidae : Cypriniformes)

2016 ◽  
Vol 42 (2) ◽  
pp. 201-208
Author(s):  
Hawa Jahan ◽  
Partha Sarathi Gope ◽  
Mohammad Shamimul Alam ◽  
Reza Md Shahjahan
Keyword(s):  
Skeletal Muscles ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Reproductive Organ ◽  
Local Market ◽  
Esterase Isozyme ◽  
Single Allele ◽  
Hind Gut ◽  
Different Tissues ◽  
Puntius Sophore

Esterase isozyme of different tissues of Puntius sophore was analyzed using 7.5 % polyacrylamide gel electrophoresis (PAGE). Fish specimens were collected from local market. The tissues used were taken from different muscles, stomach, fore-, mid- and hind-gut, liver, eyes, gill, heart, kidney, fore-, mid- and hind- brain, eggs and ovary. Six different esterase bands were detected, named Est-1, Est-2, Est-3, Est-4, Est-5 and Est-6 and their relative mobility were 1.0, 0.84, 0.62, 0.33, 0.26 and 0.13 respectively, each of them representing a single allele. The highest esterase activity was found in liver, followed by gill, kidney, heart, brain, intestine, stomach, eye, reproductive organ and skeletal muscles as detected in the staining intensity. Staining intensity of Est-4 and Est-5 was higher and Est-6 was the least stained in all the tissues. Asiat. Soc. Bangladesh, Sci. 42(2): 201-208, December 2016

Calmodulin-mediated gating of lens gap junction channels in vesicles

1984 ◽  
Vol 42 ◽  
pp. 134-137
Author(s):  
Camillo Peracchia ◽  
Stephen J. Girsch
Keyword(s):  
Gap Junctions ◽  
Freeze Fracture ◽  
Orthogonal Arrays ◽  
Eye Lens ◽  
Lens Fiber ◽  
Cell Coupling ◽  
Particle Spacing ◽  
Fiber Cells ◽  
Gap Junction Channels ◽  
Communicating Junctions

The fiber cells of eye lens communicate directly with each other by exchanging ions, dyes and metabolites. In most tissues this type of communication (cell coupling) is mediated by gap junctions. In the lens, the fiber cells are extensively interconnected by junctions. However, lens junctions, although morphologically similar to gap junctions, differ from them in a number of structural, biochemical and immunological features. Like gap junctions, lens junctions are regions of close cell-to-cell apposition. Unlike gap junctions, however, the extracellular gap is apparently absent in lens junctions, such that their thickness is approximately 2 nm smaller than that of typical gap junctions (Fig. 1,c). In freeze-fracture replicas, the particles of control lens junctions are more loosely packed than those of typical gap junctions (Fig. 1,a) and crystallize, when exposed to uncoupling agents such as Ca++, or H+, into pseudo-hexagonal, rhombic (Fig. 1,b) and orthogonal arrays with a particle-to-particle spacing of 6.5 nm. Because of these differences, questions have been raised about the interpretation of the lens junctions as communicating junctions, in spite of the fact that they are the only junctions interlinking lens fiber cells.

On the assembly and structural modulation of lens fiber junctions

1984 ◽  
Vol 42 ◽  
pp. 110-113
Author(s):  
E.L. Benedetti ◽  
I. Dunia ◽  
Do Ngoc Lien ◽  
O. Vallon ◽  
D. Louvard ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Natural History ◽  
Intercellular Junctions ◽  
Eye Lens ◽  
Membrane Domains ◽  
Functional Polymorphism ◽  
Lens Fiber ◽  
The Past ◽  
Structural Modulation ◽  
Biochemical Difference

In the eye lens emerging molecular and structural patterns apparently cohabit with the remnants of the past. The lens in a rather puzzling fashion sums up its own natural history and even transient steps of the differentiation are memorized. A prototype of this situation is well outlined by the study of the lenticular intercellular junctions. These membrane domains exhibit structural, biochemical and perhaps functional polymorphism reflecting throughout life the multiple steps of the differentiation of the epithelium into fibers and of the ageing process of the lenticular cells.The most striking biochemical difference between the membrane derived from the epithelium and from the fibers respectively, concerns the presence of the 26,000 molecular weight polypeptide (MP26) in the latter membranes.

A Physiologic Regulator of Collagen-Induced Platelet Aggregation: Inhibition by Clq

1981 ◽  
Vol 45 (02) ◽  
pp. 110-115 ◽  
Author(s):  
György Csákó ◽  
Eva A Suba
Keyword(s):  
Platelet Aggregation ◽  
Human Platelet ◽  
Platelet Reactivity ◽  
High Specificity ◽  
Turbidimetric Method ◽  
Specific Manner ◽  
Aggregation Inhibition ◽  
Different Tissues

SummaryPlatelet aggregations were studied by a turbidimetric method in citrated human platelet-rich plasmas (PRP) in vitro. Human Clq inhibited the aggregations caused by collagens derived from different tissues and species. Clq was needed by weight in comparable quantities to collagen for neutralizing the aggregating effect. The dependence of the inhibitory reaction on the preincubation of platelets with Clq and the differences in the occurrence of aggregating substances in supernatants of PRP triggered with collagen in the presence or absence of Clq, confirmed that Clq exerts its effect by preventing fixation of collagen to platelets. In addition, the high specificity of the inhibitory action of Clq for collagen-induced platelet aggregation was demonstrated by results obtained for testing a variety of aggregating agents in combination with Clq and/or collagen.Since normal concentrations of Clq in the blood are in the range of inhibitory doses of Clq for collagen-induced platelet aggregations in vitro and upon activation of complement Clq is known to dissociate from Cl, it is proposed that Clq may participate in a highly specific manner in regulating platelet reactivity to collagen in vivo.

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