scholarly journals Produção de biomassa micelial e enzimas lignocelulolíticas de Pleurotus spp. em meio de cultivo líquido

2021 ◽  
Vol 10 (1) ◽  
pp. e6810111406
Author(s):  
Nelma Lopes Araújo ◽  
Katielle Vieira Avelino ◽  
Marisangela Isabel Wietzikoski Halabura ◽  
Renan Alberto Marim ◽  
Adma Soraia Serea Kassem ◽  
...  

Pleurotus spp. são fungos capazes de degradar a lignocelulose graças à excreção de enzimas com diferentes aplicações industriais. O objetivo deste estudo foi avaliar a influência dos meios de cultivo na produção de biomassa micelial e na atividade de lacase, celulases e xilanase de Pleurotus spp. Pleurotus citrinopileatus U16-23, P. djamor U16-20, U16-25 e U16-28, P. eryngii U16-30, P. ostreatus U16-22 e P. pulmonarius U16-21 foram cultivados por 12 dias a 28 ºC em meio extrato de malte (EM-20 g L-1), meio definido (MD glicose 10 g L-1 e extrato de levedura 2 g L-1), ou em meio bagaço de cana-de-açúcar (BC) igual ao MD, mas substituindo glicose por bagaço (50 g L-1). O maior crescimento ocorreu no meio EM e P. djamor U16-25 a cepa que gerou mais biomassa (7,5 ± 0,1 g L-1). A maioria das cepas apresentou maior atividade de lacase no meio MD e as atividades maiores foram de P. djamor U16-25 (22243 ± 745 U L-1) e P. pulmonarius (20924 ± 46 U L-1). P. citrinopileatus e P. djamor U16-25 não produziram celulases, mas P. djamor U16-20 e P. eryngii produziram as maiores atividades de celulase nos meios EM e MD. Todas as cepas produziram xilanase em e as maiores atividades foram observadas no meio EM e P. djamor U16-28 (67154 ± 1597 U L-1) foi o maior produtor. Pleurotus djamor U16-25 se destacou como produtor de lacase e de xilanase livre de celulase revelando potencial para aplicação na indústria de papel e celulose.

2008 ◽  
Vol 54 (5) ◽  
pp. 366-370 ◽  
Author(s):  
Erbil Kalmış ◽  
Nuri Azbar ◽  
Fatih Kalyoncu

Biological decolorization of Benazol Black ZN, a reactive azo-type textile dyestuff, was comparatively studied using 3 different commercial-type white rot fungi strains ( Pleurotus ostreatus , Pleurotus cornucopiae var. citrinopileatus, Pleurotus djamor , and 2 wild types of P. ostreatus (MCC07 and MCC20) isolated from the nature. The initial dye concentrations in the medium were 500 and 1000 mg·L–1. All the organisms studied decolorized Benazol Black ZN to varying degrees. At low dye concentration, both commercial and wild type of P. ostreatus resulted in the best decolorization, conversely, wild-type P. ostreatus (MCC07) was found to be much more robust against increasing dye concentration and provided the best decolorization efficiency at high dye concentration.


2013 ◽  
Vol 42 (1) ◽  
pp. 125-128
Author(s):  
Mariusz Gapiński ◽  
Wanda Woźniak ◽  
Mirosława Ziombra ◽  
Joanna Murawska

The research presents the results of oyster mycelium growth on the liquid medium. The growth of 4-mycelium genius: <em>Pleurotus citrinopileatus</em> Singer, <em>Pleurotus djamor</em> Fries, Boedjin, <em>Pleurotus erynii</em> Fr. Kumm. and <em>Pleurotus precoce</em> Fr. Quel was tested. The quality and quantity of mycelium was assumed.


2019 ◽  
Vol 20 (15) ◽  
pp. 1309-1320 ◽  
Author(s):  
Ahmed M. Younis ◽  
Marwa M. Abdel-Aziz ◽  
Mohamed Yosri

Background:: Mushrooms are deemed as a special delicacy in many countries. They are considered an important cuisine due to their bioactive ingredients and possible health benefits. Methods: Herein, we measured selected biological properties of methanol extracts of Pleurotus citrinopileatus and Boletus edulis fruiting bodies including; in vitro antimicrobial activity, anti-α- glucosidase activity, antioxidant activity, anti-lipase activity and cytotoxic activity against different cancer cells and normal cells. Results: B. edulis methanol extracts showed high antimicrobial and anti-α-glucosidase activity. In contrast, P. citrinopileatus methanol extracts showed superior antioxidant activity indicated by (1,1- diphenyl-2-picrylhydrazyl) DPPH radical scavenging with half maximal inhibitory concentration of IC50 37.4 µg/ml, anti-lipase activities with IC50 65.2 µg/ml and high cytotoxicity activity against HepG2 and HeLa cell lines with IC50 22.8 and 36.7 µg/ml, respectively. Flow cytometric analysis of the cell cycle was used to show apoptotic effects of methanol extracts against HepG2 and HeLa cells. Conclusion: P. citrinopileatus and B. edulis methanolic extracts appear to contain biologically active compounds that might be used to treat some common human diseases.


2011 ◽  
Vol 7 (4) ◽  
pp. 478-484 ◽  
Author(s):  
Fabiana F.M. De Barba ◽  
Marcia L.L. Silveira ◽  
Barbara U. Piloni ◽  
Sandra A. Furlan ◽  
Mauro S.L. Pinho

2019 ◽  
Vol 222 ◽  
pp. 114990 ◽  
Author(s):  
Gajendra Nath Maity ◽  
Prasenjit Maity ◽  
Indranil Choudhuri ◽  
Nandan Bhattacharyya ◽  
Krishnendu Acharya ◽  
...  

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